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For field testing, the most important requirements are portability, sensitivity, non-invasiveness, and quick results. Motivated by this problem, a point of care (POC) test based on lateral flow assay (LFA) was developed for the detection of cocaine (COC) and methamphetamine (MET) in saliva which has been selected as the matrix for this study due to its rapid and non-invasive collection process. In the design strategy of an LFA test, the use of gold nanoparticles (AuNPs) with strong optical properties has been combined with the advantages of selecting aptamers under in vitro conditions, making it a highly specific and stable recognition probe for the detection of small molecules in saliva. The developed aptamer-based LFA in a competitive format, was able to detect COC and MET in synthetic saliva at concentrations as low as 5.0 ng\/mL. After analytical performance studies, the test system also detected COC and MET in real patient samples, which was verified by chromatographic methods.<\/jats:p>","DOI":"10.3390\/bios15010031","type":"journal-article","created":{"date-parts":[[2025,1,9]],"date-time":"2025-01-09T05:17:08Z","timestamp":1736399828000},"page":"31","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Non-Invasive Point-of-Care Detection of Methamphetamine and Cocaine via Aptamer-Based Lateral Flow Test"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1567-9899","authenticated-orcid":false,"given":"Bilge","family":"Erkocyigit","sequence":"first","affiliation":[{"name":"Department of Biotechnology, Institute of Natural Sciences, Ege University, 35040 Izmir, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4550-3889","authenticated-orcid":false,"given":"Ezgi","family":"Man","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Science, Ege University, 35040 Izmir, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-4001-2622","authenticated-orcid":false,"given":"Ece","family":"Efecan","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Institute of Natural Sciences, Ege University, 35040 Izmir, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-0722-6955","authenticated-orcid":false,"given":"Ozge","family":"Ozufuklar","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Institute of Natural Sciences, Ege University, 35040 Izmir, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deniz","family":"Devecioglu","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, Ataturk Educational and Research Hospital, Katip Celebi University, 35360 Izmir, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Basak","family":"Bagci","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, Ataturk Educational and Research Hospital, Katip Celebi University, 35360 Izmir, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3808-1956","authenticated-orcid":false,"given":"Ebru","family":"Aldemir","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, Faculty of Medicine, Izmir Tinaztepe University, 35400 Izmir, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hakan","family":"Coskunol","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, Faculty of Medicine, Ege University, 35040 Izmir, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6676-4888","authenticated-orcid":false,"given":"Serap","family":"Evran","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Science, Ege University, 35040 Izmir, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2381-9775","authenticated-orcid":false,"given":"Emine","family":"Guler Celik","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, Faculty of Engineering, Ege University, 35040 Izmir, T\u00fcrkiye"},{"name":"Ege Science Pro Scientific Research Inc., 35040 Izmir, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Levine, B.S., and Kerrigan, S. 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