{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T10:04:54Z","timestamp":1786701894839,"version":"build-2736575974"},"reference-count":42,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2025,1,24]],"date-time":"2025-01-24T00:00:00Z","timestamp":1737676800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Philippine Council for Health Research and Development of the Department of Science and Technology (DOST-PCHRD)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>Annually, the Philippines is burdened by a high number of infections and deaths due to Dengue. This disease is caused by the Dengue virus (DENV) and is transmitted from one human host to another by the female Aedes aegypti mosquito. Being a developing country, most of the high-risk areas in the Philippines are resource-limited and cannot afford equipment for detection and monitoring. Moreover, traditional clinical diagnoses of DENV infection are costly and time-consuming and require expertise. Hence, it is important to establish effective vector control and surveillance measures. In this study, we developed a DNA-based nanobiosensor for the colorimetric detection of Dengue virus serotype 2 (DENV-2) synthetic target DNA (stDNA S2) using gold nanoparticles (AuNPs). We successfully functionalized dextrin-capped gold nanoparticles with the designed DENV-2 oligonucleotide probes. The detection of the complementary stDNA S2, indicated by the pink-colored solution, was successfully performed within 15 min using 0.40 M NaCl solution. We were able to detect up to 36.14 ng\/\u03bcL of stDNA S2 with some cross-reactivity observed with one non-complementary target. We believe that our study offers a basis for developing nanobiosensors for other DENV serotypes.<\/jats:p>","DOI":"10.3390\/bios15020071","type":"journal-article","created":{"date-parts":[[2025,1,24]],"date-time":"2025-01-24T03:26:31Z","timestamp":1737689191000},"page":"71","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["DNA-Based Nanobiosensor for the Colorimetric Detection of Dengue Virus Serotype 2 Synthetic Target Oligonucleotide"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-7885-6772","authenticated-orcid":false,"given":"Michael Sandino C.","family":"Flores","sequence":"first","affiliation":[{"name":"Institute of Chemistry, College of Arts and Sciences, University of the Philippines Los Ba\u00f1os, College, Los Ba\u00f1os 4031, Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evangelyn C.","family":"Alocilja","sequence":"additional","affiliation":[{"name":"Nano-Biosensors Lab, Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA"},{"name":"Global Alliance for Rapid Diagnostics, Michigan State University, East Lansing, MI 48824, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Divina M.","family":"Amalin","sequence":"additional","affiliation":[{"name":"Department of Biology, College of Science, De La Salle University Manila, Taft Avenue, Malate, Manila 1004, Philippines"},{"name":"Institute of Biological Control, De La Salle University-Laguna Campus, Bi\u00f1an City 4024, Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4813-3967","authenticated-orcid":false,"given":"Mae Joanne B.","family":"Aguila","sequence":"additional","affiliation":[{"name":"Institute of Chemistry, College of Arts and Sciences, University of the Philippines Los Ba\u00f1os, College, Los Ba\u00f1os 4031, Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4026-2051","authenticated-orcid":false,"given":"Marynold V.","family":"Purificacion","sequence":"additional","affiliation":[{"name":"Institute of Chemistry, College of Arts and Sciences, University of the Philippines Los Ba\u00f1os, College, Los Ba\u00f1os 4031, Philippines"},{"name":"Institute of Plant Breeding, College of Agriculture and Food Science, University of the Philippines Los Ba\u00f1os, College, Los Ba\u00f1os 4031, Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florinia E.","family":"Merca","sequence":"additional","affiliation":[{"name":"Institute of Chemistry, College of Arts and Sciences, University of the Philippines Los Ba\u00f1os, College, Los Ba\u00f1os 4031, Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2508-4071","authenticated-orcid":false,"given":"Ma. Carmina C.","family":"Manuel","sequence":"additional","affiliation":[{"name":"Genetics and Molecular Biology Division, Institute of Biological Sciences, College of Arts and Sciences, University of the Philippines Los Ba\u00f1os, College, Los Ba\u00f1os 4031, Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6783-2440","authenticated-orcid":false,"given":"Mark Pierre S.","family":"Dimamay","sequence":"additional","affiliation":[{"name":"Research and Biotechnology Division, St. Luke\u2019s Medical Center, Quezon City 1112, Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8645-4243","authenticated-orcid":false,"given":"Ma. Anita M.","family":"Bautista","sequence":"additional","affiliation":[{"name":"Functional Genomics Laboratory, National Institute of Molecular Biology and Biotechnology, University of the Philippines Diliman, Quezon City 1101, Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lilia M.","family":"Fernando","sequence":"additional","affiliation":[{"name":"Institute of Chemistry, College of Arts and Sciences, University of the Philippines Los Ba\u00f1os, College, Los Ba\u00f1os 4031, Philippines"},{"name":"Institute of Crop Science, College of Agriculture and Food Science, University of the Philippines Los Ba\u00f1os, College, Los Ba\u00f1os 4031, Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Sim, S., and Hibberd, M.L. (2016). 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