{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T07:06:35Z","timestamp":1792047995512,"version":"4.4.0"},"reference-count":40,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2025,2,17]],"date-time":"2025-02-17T00:00:00Z","timestamp":1739750400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82173798"],"award-info":[{"award-number":["82173798"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81803499"],"award-info":[{"award-number":["81803499"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2022zxy001"],"award-info":[{"award-number":["2022zxy001"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Construction Project of Integrated TCM and Western Medicine Research Platform","award":["82173798"],"award-info":[{"award-number":["82173798"]}]},{"name":"Construction Project of Integrated TCM and Western Medicine Research Platform","award":["81803499"],"award-info":[{"award-number":["81803499"]}]},{"name":"Construction Project of Integrated TCM and Western Medicine Research Platform","award":["2022zxy001"],"award-info":[{"award-number":["2022zxy001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>Acetylcholinesterase inhibitors (AChEIs), particularly donepezil, are commonly used to treat mild-to-moderate Alzheimer\u2019s disease (AD). However, drug accumulation during long-term use could change AChE activity and content, leading to peripheral side effects and prompting medication discontinuation. However, there are a lack of methods to simultaneously determine the content and catalytic activity of AChE. By using phosphatidylinositol-specific phospholipase C to strip AChE from erythrocyte surfaces, we developed a novel method combining surface plasmon resonance and fluorescence detection for the simultaneous detection of AChE content and activity, producing stable, reliable, and accurate results. The established determination range spans from 263.37 ng\/mL to 3000 ng\/mL (4.05 nM to 46.15 nM) for concentration, and from 39.02 mU\/mL to 1000 mU\/mL for activity. Compared to traditional methods, this approach simplifies operations, reduces detection time, expands the dynamic range, and lowers detection limits, potentially advancing AChE-related research and supporting clinical diagnostics and drug development.<\/jats:p>","DOI":"10.3390\/bios15020118","type":"journal-article","created":{"date-parts":[[2025,2,17]],"date-time":"2025-02-17T07:48:22Z","timestamp":1739778502000},"page":"118","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Dual Monitoring of Blood Acetylcholinesterase Content and Catalytic Activity Utilizing Fluorometry-Integrated Surface Plasmon Resonance"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-9006-4985","authenticated-orcid":false,"given":"Yuanyuan","family":"Xie","sequence":"first","affiliation":[{"name":"School of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifei","family":"Hou","sequence":"additional","affiliation":[{"name":"Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengwei","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Chemical Biology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China"},{"name":"Clinical Trial Center of Zhongnan Hospital, Wuhan University, Wuhan 430071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongzhuan","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China"},{"name":"Shuguang Lab of Future Health, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China"},{"name":"Shanghai Frontiers Science Center of TCM Chemical Biology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Chemical Biology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lanxue","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Chemical Biology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianrong","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China"},{"name":"Shuguang Lab of Future Health, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China"},{"name":"Shanghai Frontiers Science Center of TCM Chemical Biology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"110726","DOI":"10.1016\/j.isci.2024.110726","article-title":"Protective role of acetylcholine and the cholinergic system in the injured heart","volume":"27","author":"Wang","year":"2024","journal-title":"iScience"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1246","DOI":"10.1111\/bjd.15808","article-title":"Neural control of sweat secretion: A review","volume":"178","author":"Hu","year":"2018","journal-title":"Br. 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