{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T09:10:15Z","timestamp":1765357815507,"version":"build-2065373602"},"reference-count":64,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T00:00:00Z","timestamp":1743120000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The Gottfried and Julia Bangerter-Rhyner-Foundation","award":["0003\/2021-0097\/2022","407240_177354","834402"],"award-info":[{"award-number":["0003\/2021-0097\/2022","407240_177354","834402"]}]},{"DOI":"10.13039\/501100001711","name":"Swiss National Science Foundation","doi-asserted-by":"publisher","award":["0003\/2021-0097\/2022","407240_177354","834402"],"award-info":[{"award-number":["0003\/2021-0097\/2022","407240_177354","834402"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Swiss Nano-science Institute (SNI)","award":["0003\/2021-0097\/2022","407240_177354","834402"],"award-info":[{"award-number":["0003\/2021-0097\/2022","407240_177354","834402"]}]},{"name":"Cleven Foundation","award":["0003\/2021-0097\/2022","407240_177354","834402"],"award-info":[{"award-number":["0003\/2021-0097\/2022","407240_177354","834402"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>Recent advancements in nanomechanical microcantilever biosensors open new possibilities for clinical applications, permitting precise analysis of molecular interactions. The technology enables tracking gene expression, molecular conformational changes, antibody binding and antibiotic resistance. In particular, hybridization of DNA or RNA extracted from biopsies and whole blood from patients has led to significant advancements in diagnostics of critical medical conditions, e.g., cancer, bacteraemia and sepsis, utilizing rapid, sensitive, and label-free detection. Direct diagnosis from patient samples is a decisive advantage over competitive methods circumventing elaborate and time-consuming purification, amplification and cultivation procedures prior to analysis. Here, recent developments are presented from simple DNA hybridization of synthesized oligonucleotides to RNA material obtained from patients\u2019 blood samples, highlighting technological advancements in diagnostic applications, such as detection of pathogens and disease biomarkers. We envisage our method to be a significant input to rapid, early and sensitive diagnosis directly from patients\u2019 blood without requirements for amplification or cultivation. This would represent a paradigm shift in diagnostics, as no competing method currently exists.<\/jats:p>","DOI":"10.3390\/bios15040217","type":"journal-article","created":{"date-parts":[[2025,3,31]],"date-time":"2025-03-31T02:50:16Z","timestamp":1743389416000},"page":"217","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Ultra-Sensitive Biosensors for Medical Applications Based on Nanomechanics: From Detection of Synthetic Biomolecules to Analysis of Sepsis in Pediatric Patients"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4826-1245","authenticated-orcid":false,"given":"Fran\u00e7ois","family":"Huber","sequence":"first","affiliation":[{"name":"Swiss Nanoscience Institute (SNI), Department of Physics, University of Basel, CH-4056 Basel, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0012-2564","authenticated-orcid":false,"given":"Hans Peter","family":"Lang","sequence":"additional","affiliation":[{"name":"Swiss Nanoscience Institute (SNI), Department of Physics, University of Basel, CH-4056 Basel, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-3853-1276","authenticated-orcid":false,"given":"Andrea","family":"Marten","sequence":"additional","affiliation":[{"name":"University Children\u2019s Hospital Basel (UKBB), Department of Medicine, University of Basel, CH-4056 Basel, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia Anna","family":"Bielicki","sequence":"additional","affiliation":[{"name":"University Children\u2019s Hospital Basel (UKBB), Department of Medicine, University of Basel, CH-4056 Basel, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6385-3412","authenticated-orcid":false,"given":"Ernst","family":"Meyer","sequence":"additional","affiliation":[{"name":"Swiss Nanoscience Institute (SNI), Department of Physics, University of Basel, CH-4056 Basel, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christoph","family":"Gerber","sequence":"additional","affiliation":[{"name":"Swiss Nanoscience Institute (SNI), Department of Physics, University of Basel, CH-4056 Basel, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1088\/0957-4484\/15\/8\/002","article-title":"Rapid functionalization of cantilever array sensors by inkjet printing","volume":"15","author":"Bietsch","year":"2004","journal-title":"Nanotechnology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/S1369-7021(05)00792-3","article-title":"Cantilever array sensors","volume":"8","author":"Lang","year":"2005","journal-title":"Mater Today"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1016\/S0006-3495(00)76351-X","article-title":"Immobilization of Nucleic Acids at Solid Surfaces: Effect of Oligonucleotide Length on Layer Assembly","volume":"79","author":"Steel","year":"2000","journal-title":"Biophys. J."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"7435","DOI":"10.3390\/s140407435","article-title":"First-Principles Surface Stress Calculations and Multiscale Deformation Analysis of a Self-Assembled Monolayer Adsorbed on a Micro-Cantilever","volume":"14","author":"Shih","year":"2014","journal-title":"Sensors"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1266","DOI":"10.1088\/1742-6596\/61\/1\/250","article-title":"Molecular mechanics modelling and simulation of the adsorption-induced surface stress in micro-nano-cantilever sensors","volume":"61","author":"Wu","year":"2007","journal-title":"J. Phys. Conf. Ser."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"3133","DOI":"10.1021\/acssensors.0c01216","article-title":"Cantilever Sensors for Rapid Optical Antimicrobial Sensitivity Testing","volume":"5","author":"Bennett","year":"2020","journal-title":"ACS Sens."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2998","DOI":"10.1021\/acs.jcim.0c00146","article-title":"Multiscale Modeling Reveals the Cause of Surface Stress Change on Microcantilevers Due to Alkanethiol SAM Adsorption","volume":"60","author":"Zhao","year":"2020","journal-title":"J. Chem. Inf. Model."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1126\/science.288.5464.316","article-title":"Translating Biomolecular Recognition into Nanomechanics","volume":"288","author":"Fritz","year":"2000","journal-title":"Science"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"6494","DOI":"10.1021\/la205066h","article-title":"Optimization of DNA Hybridization Efficiency by pH-Driven Nanomechanical Bending","volume":"28","author":"Zhang","year":"2012","journal-title":"Langmuir"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Huber, F., Lang, H.P., Heller, S., Bielicki, J.A., Gerber, C., Meyer, E., and Egli, A. (2022). Rapid Bacteria Detection from Patients\u2019 Blood Bypassing Classical Bacterial Culturing. Biosensors, 12.","DOI":"10.3390\/bios12110994"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1002\/(SICI)1099-1352(199901\/02)12:1<3::AID-JMR441>3.0.CO;2-6","article-title":"Isothermal titration calorimetry and differential scanning calorimetry as complementary tools to investigate the energetics of biomolecular recognition","volume":"12","author":"Jelesarov","year":"1999","journal-title":"J. Mol. Recognit."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"7104","DOI":"10.1021\/ac052171y","article-title":"Nanomechanical Detection of DNA Melting on Microcantilever Surfaces","volume":"78","author":"Raorane","year":"2006","journal-title":"Anal. Chem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"9783","DOI":"10.1073\/pnas.152330199","article-title":"Multiple label-free biodetection and quantitative DNA-binding assays on a nanomechanical cantilever array","volume":"99","author":"McKendry","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1599","DOI":"10.1016\/j.bios.2005.07.018","article-title":"Label free analysis of transcription factors using microcantilever arrays","volume":"8","author":"Huber","year":"2006","journal-title":"Biosens. Bioelectron."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"8602","DOI":"10.1073\/pnas.95.15.8602","article-title":"Kinetics of conformational fluctuations in DNA hairpin-loops","volume":"95","author":"Bonnet","year":"1998","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1088\/0957-4484\/14\/1\/319","article-title":"Label-free protein assay based on a nanomechanical cantilever array","volume":"14","author":"Arntz","year":"2003","journal-title":"Nanotechnology"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/S0956-5663(01)00276-7","article-title":"Characterisation of an antibody coated microcantilever as a potential immuno-based biosensor","volume":"17","author":"Grogan","year":"2002","journal-title":"Biosens. Bioelectron."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.snb.2014.03.023","article-title":"Microcantilever array biosensors for detection and recognition of Gram-negative bacterial endotoxins","volume":"198","author":"Katarzyna","year":"2014","journal-title":"Sens. Actuators B Chem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"14587","DOI":"10.1073\/pnas.0504917102","article-title":"A label-free immunosensor array using single-chain antibody fragments","volume":"102","author":"Backmann","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2970","DOI":"10.1529\/biophysj.105.072934","article-title":"Conformational Change of Bacteriorhodopsin Quantitatively Monitored by Microcantilever Sensors","volume":"90","author":"Braun","year":"2006","journal-title":"Biophys. J."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1134\/S1990793112060127","article-title":"A Study of the Photoinduced Conformational Mobility of Spin-Labeled Regenerated Rhodopsin by ESR Spectroscopy","volume":"6","author":"Shelyakin","year":"2012","journal-title":"Russ. J. Phys. Chem. B"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Etayash, H., Jiang, K., Azmi, S., Thundat, T., and Kaur, K. (2015). Real-time Detection of Breast Cancer Cells Using Peptidefunctionalized Microcantilever Arrays. Sci. Rep., 5.","DOI":"10.1038\/srep13967"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3034","DOI":"10.1021\/acssensors.9b01604","article-title":"Microcantilever Array Biosensor for Simultaneous Detection of Carcinoembryonic Antgens and \u03b1-Fetoprotein Based on Real-Time Monitoring of the Profile of Cantilever","volume":"4","author":"Li","year":"2019","journal-title":"ACS Sens."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Wang, J., Wang, L., Zhu, Y., Zhang, J., Liao, J., Wang, S., Yang, J., and Yang, F. (2016). A high accuracy cantilever array sensor for early liver cancer diagnosis. Biomed. Microdevices, 18.","DOI":"10.1007\/s10544-016-0132-5"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"109558","DOI":"10.1016\/j.enzmictec.2020.109558","article-title":"Micro\/Nano fabricated cantilever-based biosensor platform: A review and recent progress","volume":"139","author":"Basua","year":"2020","journal-title":"Enzyme Microb. Technol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1038\/nrclinonc.2011.69","article-title":"BRAF targeted therapy changes the treatment paradigm in melanoma","volume":"8","author":"Ribas","year":"2011","journal-title":"Nat. Rev. Clin. Oncol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1038\/nnano.2012.263","article-title":"Direct detection of a BRAF mutation in total RNA from melanoma cells using cantilever arrays","volume":"8","author":"Huber","year":"2013","journal-title":"Nat. Nanotechnol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"5373","DOI":"10.1021\/acs.nanolett.6b01513","article-title":"Fast Diagnostics of BRAF Mutations in Biopsies from Malignant Melanoma","volume":"16","author":"Huber","year":"2016","journal-title":"Nano Lett."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1016\/S0140-6736(19)32989-7","article-title":"Global, regional, and national sepsis incidence and mortality, 1990\u20132017: Analysis for the Global Burden of Disease Study","volume":"395","author":"Rudd","year":"2020","journal-title":"Lancet"},{"key":"ref_30","unstructured":"Gorina, Y., and Kramarow, E.A. (2024, December 01). QuickStats: Sepsis-Related Death Rates Among Persons Aged \u226565 Years, by Age Group and Sex\u2014National Vital Statistics System, United States, Available online: https:\/\/www.cdc.gov\/mmwr\/volumes\/72\/wr\/mm7238a5.htm?s_cid=mm7238a5_w."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1177\/2211068214567345","article-title":"Sepsis Pathogen Identification","volume":"20","author":"Chun","year":"2015","journal-title":"J. Lab. Autom."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"547","DOI":"10.7326\/M19-1696","article-title":"Blood Culture Results Before and After Antimicrobial Administration in Patients with Severe Manifestations of Sepsis A Diagnostic Study","volume":"171","author":"Cheng","year":"2019","journal-title":"Ann. Intern. Med."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Behera, B., Vishnu, G.K.A., Chatterjee, S., Sitaramgupta, V.S.N.V., Sreekumar, N., Nagabhushan, A., Rajendran, N., Prathik, B.H., and Pandya, H.J. (2019). Emerging technologies for antibiotic susceptibility testing. Biosens. Bioelectron., 142.","DOI":"10.1016\/j.bios.2019.111552"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Ferran, P.V., Villa, R., and Alvarez, M. (2020). Nanomechanical Sensors as a Tool for Bacteria Detection and Antibiotic Susceptibility Testing. Front. Mech., 6.","DOI":"10.3389\/fmech.2020.00044"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2037","DOI":"10.1038\/s41467-024-46213-y","article-title":"Accurate and rapid antibiotic susceptibility testing using a machine learning-assisted nanomotion technology platform","volume":"15","author":"Sturm","year":"2024","journal-title":"Nat. Commun."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1862","DOI":"10.1364\/BOE.484015","article-title":"Fiber-integrated cantilever-based nanomechanical biosensors as a tool for rapid antibiotic susceptibility testing","volume":"14","author":"Zhou","year":"2023","journal-title":"Biomed. Opt. Express"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"023105","DOI":"10.1088\/1674-4926\/44\/2\/023105","article-title":"Microcantilever sensors for biochemical detection","volume":"44","author":"Wang","year":"2023","journal-title":"J. Semicond."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1567","DOI":"10.1021\/ac0012748","article-title":"Cantilever-Based Optical Deflection Assay for Discrimination of DNA Single-Nucleotide Mismatches","volume":"73","author":"Hansen","year":"2001","journal-title":"Anal. Chem."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2000066","DOI":"10.1002\/gch2.202000066","article-title":"Rapid and Ultrasensitive Detection of Mutations and Genes Relevant to Antimicrobial Resistance in Bacteria","volume":"5","author":"Huber","year":"2021","journal-title":"Glob. Chall."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1038\/nnano.2008.275","article-title":"Nanomechanical detection of antibiotic\u2013mucopeptide binding in a model for superbug drug resistance","volume":"3","author":"Watari","year":"2008","journal-title":"Nat. Nanotechnol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"e00192-17","DOI":"10.1128\/mSphere.00192-17","article-title":"Role of AmpR in the High Expression of the Plasmid-Encoded AmpC \u03b2-Lactamase CFE-1","volume":"2","author":"Nakano","year":"2017","journal-title":"mSphere"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1021\/la0521645","article-title":"Chemomechanics of Surface Stresses Induced by DNA Hybridization","volume":"22","author":"Stachowiak","year":"2006","journal-title":"Langmuir"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1111\/1469-0691.12180","article-title":"Update on blood cultures: How to obtain, process, report, and interpret","volume":"19","author":"Kirn","year":"2013","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"103","DOI":"10.3103\/S0891416812030056","article-title":"Popular molecular markers in bacteria","volume":"27","author":"Liu","year":"2012","journal-title":"Mol. Gen. Microbiol. Virol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1038\/nnano.2006.134","article-title":"Rapid and label-free nanomechanical detection of biomarker transcripts in human RNA","volume":"1","author":"Zhang","year":"2006","journal-title":"Nat. Nanotechnol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1016\/j.ultramic.2006.11.001","article-title":"A complete analysis of the laser beam deflection systems used in cantilever-based systems","volume":"107","author":"Beaulieu","year":"2007","journal-title":"Ultramicroscopy"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Huen, K., Lizarraga, D., Kogut, K., Eskenazi, B., and Holland, N. (2019). Age-Related Differences in miRNA Expression in Mexican-American Newborns and Children. Int. J. Environ. Res. Public Health, 4.","DOI":"10.3390\/ijerph16040524"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Trevethan, R. (2017). Sensitivity, Specificity, and Predictive Values: Foundations, Pliabilities, and Pitfalls in research and Practice. Front. Public Health, 5.","DOI":"10.3389\/fpubh.2017.00307"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Calderaro, A., and Chezzi, C. (2024). MALDI-TOF MS: A Reliable Tool in the Real Life of the Clinical Microbiology. Microorganisms, 12.","DOI":"10.3390\/microorganisms12020322"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1093\/ajcp\/aqad160","article-title":"Use of matrix-assisted laser desorption ionization time of flight (MALDI-TOF) to detect antibiotic resistance in bacteria","volume":"161","author":"Viboud","year":"2024","journal-title":"Am. J. Clin. Pathol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"465262","DOI":"10.1016\/j.chroma.2024.465262","article-title":"Machine learning and matrix-assisted laser desorption\/ionization time-of-flight mass spectra for antimicrobial resistance prediction: A systematic review of recent advancements and future development","volume":"1734","year":"2024","journal-title":"J. Chromatogr. A"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"105296","DOI":"10.1016\/j.micinf.2024.105296","article-title":"Present and future perspectives on mass spectrometry for clinical microbiology","volume":"26","author":"Gant","year":"2024","journal-title":"Microbes Infect."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"124133","DOI":"10.1016\/j.talanta.2022.124133","article-title":"Recent advances of fluorescent sensors for bacteria detection-A review","volume":"254","author":"Zhang","year":"2023","journal-title":"Talanta"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"343428","DOI":"10.1016\/j.aca.2024.343428","article-title":"Graphene oxide-based fluorescent biosensors for pathogenic bacteria detection: A review","volume":"1337","author":"Liu","year":"2025","journal-title":"Anal. Chim. Acta"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.aca.2020.05.012","article-title":"Highly sensitive and universaldetection strategy based on a colorimetric assay using target-specific hetero-geneous sandwich DNA aptamer","volume":"1123","author":"Kang","year":"2020","journal-title":"Anal. Chim. Acta"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.bios.2017.02.032","article-title":"Label and label-free based surface-enhanced Raman scattering for pathogen bacteria detection: A review","volume":"94","author":"Liu","year":"2017","journal-title":"Biosens. Bioelectron."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Lopez-Tellez, J., Ramirez-Montez, S., Ferreira, T.A., Santos, E.M., and Rodriguez, J.A. (2022). Application of Voltammetric Sensors for Pathogen Bacteria Detection: A Review. Chemosensors, 10.","DOI":"10.3390\/chemosensors10100424"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.trac.2015.10.015","article-title":"Ligands for label-free detection of whole bacteria on biosensors: A review","volume":"79","author":"Templier","year":"2016","journal-title":"Trends Analyt. Chem."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Paczesny, J., Richter, L., and Ho\u0142yst, R. (2020). Recent Progress in the Detection of Bacteria Using Bacteriophages: A Review. Viruses, 12.","DOI":"10.3390\/v12080845"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"111918","DOI":"10.1016\/j.microc.2024.111918","article-title":"A review of new emergoiing biosensors based on bacteria-imprinted polymers towards pathogenic bacteria: Promising new tools for selective detection","volume":"207","author":"Hsu","year":"2024","journal-title":"Microchem. J."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"28487","DOI":"10.1039\/D4RA04038D","article-title":"Bioreceptor modified electrochemical biosensors for the detection of life threating pathogenic bacteria: A review","volume":"14","author":"Khaleque","year":"2024","journal-title":"RSC Adv."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1186\/s12985-024-02513-9","article-title":"Advances in nucleic acid aptamer-based detection of respiratory virus and bacteria: A mini review","volume":"21","author":"Feng","year":"2024","journal-title":"Virol. J."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Tang, Y., Li, Y., Chen, P., Zhong, S., and Yang, Y. (2025). Nucleic Acid Aptamer-Based Sensors for Bacteria Detection: A Review. BioEssays, 47.","DOI":"10.1002\/bies.202400111"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"125585","DOI":"10.1016\/j.talanta.2023.125585","article-title":"Colourimetric and SERS dual-mode aptasensor using Au@Ag and magnetic nanoparticles for the detection of Campylobacter jejuni","volume":"270","author":"Zhao","year":"2024","journal-title":"Talanta"}],"container-title":["Biosensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6374\/15\/4\/217\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:04:46Z","timestamp":1760029486000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6374\/15\/4\/217"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,28]]},"references-count":64,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2025,4]]}},"alternative-id":["bios15040217"],"URL":"https:\/\/doi.org\/10.3390\/bios15040217","relation":{},"ISSN":["2079-6374"],"issn-type":[{"type":"electronic","value":"2079-6374"}],"subject":[],"published":{"date-parts":[[2025,3,28]]}}}