{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T20:08:18Z","timestamp":1792872498424,"version":"4.5.0"},"reference-count":54,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T00:00:00Z","timestamp":1744070400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Carlos Chagas Filho Foundation of Research Support of the State of Rio de Janeiro","award":["010.101.029\/2018"],"award-info":[{"award-number":["010.101.029\/2018"]}]},{"name":"Carlos Chagas Filho Foundation of Research Support of the State of Rio de Janeiro","award":["301744\/2019-0"],"award-info":[{"award-number":["301744\/2019-0"]}]},{"name":"Brazilian Council for Scientific Research","award":["010.101.029\/2018"],"award-info":[{"award-number":["010.101.029\/2018"]}]},{"name":"Brazilian Council for Scientific Research","award":["301744\/2019-0"],"award-info":[{"award-number":["301744\/2019-0"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>Dengue is a neglected disease mainly affecting tropical and subtropical countries. The diagnosis of dengue fever is still a problem since most of it is made from whole or recombinant DENV proteins, which present cross-reactions with other members of the Flavivirus family. Therefore, there is still a huge demand for new diagnostic methods that provide rapid, low-cost, easy-to-use confirmation. Thus, in this study, we developed an affordable electrochemical biosensor for rapidly detecting immunoglobulin G (IgG) serological antibodies in the sera of DENV-infected patients. An identified linear B-cell epitope (DENV\/18) specific for DENV 1\u20134 serotypes recognized by IgG in patient sera was selected as a target molecule after a microarray of peptides using the SPOT-synthesis methodology. After chemical synthesis, the DENV\/18-peptide was immobilized on the surface of the working electrode of a commercially available screen-printed gold electrode (SPGE). The capture of DENV-specific IgG allowed for the formation of an immunocomplex that was measured by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) using a potassium ferrocyanide\/ferricyanide ([Fe(CN)6]3\u2212\/4\u2212) electrochemical probe. An evaluation of the biosensor\u2019s performance showed a detection limit of 100 \u00b5g mL\u22121 for the synthetic peptides (DENV\/18) and 1.21 ng mL\u22121 in CV and 0.43 ng mL\u22121 in DPV for human serum, with a sensitivity of 7.21 \u00b5A in CV and 8.79 \u00b5A in DPV. The differentiation of infected and uninfected individuals was possible even at a high dilution factor that reduced the required sample volumes to a few microliters. The final device proved suitable for diagnosing DENV by analyzing real serum samples, and the results showed good agreement with molecular biology diagnostics. The flexibility to conjugate other antigenic peptides to SPEs suggests that this technology could be rapidly adapted to diagnose other pathogens.<\/jats:p>","DOI":"10.3390\/bios15040236","type":"journal-article","created":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T05:59:00Z","timestamp":1744091940000},"page":"236","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Ultrasensitive Peptide-Based Electrochemical Biosensor for Universal Diagnostic of Dengue"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4860-4642","authenticated-orcid":false,"given":"Isis Campos","family":"Prado","sequence":"first","affiliation":[{"name":"Center for Technological Development in Health (CDTS)\/National Institute of Science and Technology for Innovation in Neglected Population Diseases (INCT-IDPN), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro 21040-900, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o Pedro Rangel da Silva","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Center for Technological Development in Health (CDTS)\/National Institute of Science and Technology for Innovation in Neglected Population Diseases (INCT-IDPN), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro 21040-900, RJ, Brazil"},{"name":"Program of Post-Graduation on Science and Biotechnology, Biology Institute, Federal Fluminense University, Niteroi 24020-141, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7047-6288","authenticated-orcid":false,"given":"Andr\u00e9 Souza","family":"Araujo","sequence":"additional","affiliation":[{"name":"Analytical Chemistry Department, Chemistry Institute, Federal Fluminense University, Niteroi 24020-141, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paloma","family":"Napole\u00e3o-P\u00eago","sequence":"additional","affiliation":[{"name":"Center for Technological Development in Health (CDTS)\/National Institute of Science and Technology for Innovation in Neglected Population Diseases (INCT-IDPN), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro 21040-900, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2172-656X","authenticated-orcid":false,"given":"Salvatore Giovanni","family":"De-Simone","sequence":"additional","affiliation":[{"name":"Center for Technological Development in Health (CDTS)\/National Institute of Science and Technology for Innovation in Neglected Population Diseases (INCT-IDPN), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro 21040-900, RJ, Brazil"},{"name":"Program of Post-Graduation on Science and Biotechnology, Biology Institute, Federal Fluminense University, Niteroi 24020-141, RJ, Brazil"},{"name":"Epidemiology and Molecular Systematics Laboratory (LEMS), Oswaldo Cruz Institut, Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro 21040-900, RJ, Brazil"},{"name":"Program of Post-Graduation on Parasitic Biology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,8]]},"reference":[{"key":"ref_1","unstructured":"(2024, March 28). 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