{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T12:49:34Z","timestamp":1793710174146,"version":"4.6.1"},"reference-count":51,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,4,20]],"date-time":"2025-04-20T00:00:00Z","timestamp":1745107200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>In the current study, the Prussian blue analog decorated with zinc oxide (PBA@ZnO) was produced using a simple chemical co-precipitation method. The nanohybrid was examined using XRD, EDX, SEM, and TEM techniques, where it exhibited a polycrystalline structure with highly intense broadening peaks. The surface morphology was observed as thin nanosheets decorated with tiny spheres. Following excitation at 360 nm, the fluorescence spectra of PBA@ZnO showed fluorescence emission at 455 nm. The developed PBA@ZnO was used to qualitatively and quantitatively assess sunset yellow (SY), where its native fluorescence was selectively quenched as SY concentrations increased. For the first time, PBA@ZnO was used as a turn-off nano-sensor for the spectrofluorimetric measurement of SY. The method\u2019s markable sensitivity was demonstrated within an SY linearity range of 50\u2013500 ng\/mL, where the limit of detection was calculated as 9.77 ng\/mL. Real sample analysis in the food industry, including samples from real food, soft drinks, and sun cream, was made possible by the detection of tiny amounts of SY. Analytical Greenness (AGREE), AGREEprep, and the complementing Green Analytical Procedure Index (Complex MoGAPI) were used to illustrate the new approach\u2019s exceptional eco-friendliness and greenness. The RGB 12 algorithm worked to demonstrate that the suggested approach is less costly, more environmentally friendly, more sustainable, analytically sound, and whiter than the ones that were previously published. In accordance with ICH principles, the suggested method was validated. This approach offers a promising way to rapidly and accurately identify and measure SY in the food industry, helping to guarantee food safety and maintain the health of customers.<\/jats:p>","DOI":"10.3390\/bios15040263","type":"journal-article","created":{"date-parts":[[2025,4,20]],"date-time":"2025-04-20T20:24:16Z","timestamp":1745180656000},"page":"263","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Smart Prussian Blue Analog Decorated with Zinc Oxide Nanohybrid: Fluorescent Sensing and Sustainability of Sunset Yellow in Food and Environment"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9958-4290","authenticated-orcid":false,"given":"Hany A.","family":"Batakoushy","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Menoufia University, Shebin Elkom 32511, Egypt"},{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Menoufia National University, 70 km Cairo-Alexandria Agricultural Road, Menoufia 32952, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0588-0073","authenticated-orcid":false,"given":"Amr K. A.","family":"Bass","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Menoufia National University, 70 km Cairo-Alexandria Agricultural Road, Menoufia 32952, Egypt"},{"name":"Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Menoufia University, Shebin Elkom 32511, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7470-7741","authenticated-orcid":false,"given":"Hassanien","family":"Gomaa","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science, Al-Azhar University, Assiut 71524, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6417-4737","authenticated-orcid":false,"given":"Sami","family":"El Deeb","sequence":"additional","affiliation":[{"name":"Institute of Medicinal and Pharmaceutical Chemistry, Technische Universitaet Braunschweig, 38106 Braunschweig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7421-1220","authenticated-orcid":false,"given":"Adel Ehab","family":"Ibrahim","sequence":"additional","affiliation":[{"name":"Natural and Medical Sciences Research Center, University of Nizwa, P.O. Box 33, Birkat Al Mauz, Nizwa 616, Oman"},{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Port Said University, Port Said 42511, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1016\/j.foodchem.2012.11.029","article-title":"A nanosilver-based spectrophotometry method for sensitive determination of tartrazine in food samples","volume":"138","author":"Sahraei","year":"2013","journal-title":"Food Chem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1007\/s00217-014-2215-y","article-title":"A non-diazotization-coupling reaction-based colorimetric determination of nitrite in tap water and milk","volume":"238","author":"Zhu","year":"2014","journal-title":"Eur. Food Res. Technol."},{"key":"ref_3","first-page":"1","article-title":"A Review on Sunset Yellow Toxicity and its Analytical Methods","volume":"1","year":"2024","journal-title":"J. Chem. Biol. Med. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"42","DOI":"10.29161\/PT.v7.i1.2019.42","article-title":"The mysterious domination of food\/Drinking water contaminants and adulterants in Bangladesh","volume":"7","author":"Mohiuddin","year":"2019","journal-title":"PharmaTutor"},{"key":"ref_5","unstructured":"Liang, J. (2022). Nutritional Toxicology, Springer."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2063","DOI":"10.1039\/D3AY01944F","article-title":"Nitrogen-doped biomass-derived carbon dots for fluorescence determination of sunset yellow","volume":"16","author":"Zhang","year":"2024","journal-title":"Anal. Methods"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.saa.2016.05.038","article-title":"Determination of sunset yellow in soft drinks based on fluorescence quenching of carbon dots","volume":"167","author":"Yuan","year":"2016","journal-title":"Spectrochim. Acta Part A Mol. Biomol. Spectrosc."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"277","DOI":"10.4236\/ajac.2022.138019","article-title":"Simultaneous determination by HPLC-UV vis of tartrazine and sunset yellow in soft drinks sold in benin","volume":"13","author":"Agbokponto","year":"2022","journal-title":"Am. J. Anal. Chem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"100183","DOI":"10.1016\/j.greeac.2024.100183","article-title":"Analytical Quality by Design aided RP-HPLC method for the estimation of Sunset Yellow in commercial food samples employing green ultrasound assisted extraction: Greenness, Blueness and Whiteness evaluation","volume":"12","author":"Chakraborty","year":"2024","journal-title":"Green Anal. Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.chroma.2005.07.024","article-title":"HPLC-MS degradation study of E110 Sunset Yellow FCF in a commercial beverage","volume":"1090","author":"Gosetti","year":"2005","journal-title":"J. Chromatogr. A"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1742","DOI":"10.1016\/j.foodchem.2012.11.084","article-title":"Determination of seven synthetic dyes in animal feeds and meat by high performance liquid chromatography with diode array and tandem mass detectors","volume":"138","author":"Zou","year":"2013","journal-title":"Food Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1016\/S0039-9140(02)00320-X","article-title":"Spectrophotometric multicomponent determination of sunset yellow, tartrazine and allura red in soft drink powder by double divisor-ratio spectra derivative, inverse least-squares and principal component regression methods","volume":"58","author":"Baydan","year":"2002","journal-title":"Talanta"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"5005","DOI":"10.20964\/2018.05.35","article-title":"Detection of sunset yellow by adsorption voltammetry at glassy carbon electrode modified with chitosan","volume":"13","author":"Nagles","year":"2018","journal-title":"Int. J. Electrochem. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"134412","DOI":"10.1016\/j.foodchem.2022.134412","article-title":"Optimization of square wave voltammetry parameters by response surface methodology for the determination of Sunset yellow using an electrochemical sensor based on Purpald\u00ae","volume":"404","author":"Calam","year":"2023","journal-title":"Food Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"43139","DOI":"10.1021\/acsomega.2c05694","article-title":"Synthesis of prussian blue analogue and its catalytic activity toward reduction of environmentally toxic nitroaromatic pollutants","volume":"7","author":"Mahmoud","year":"2022","journal-title":"ACS Omega"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"108247","DOI":"10.1007\/s11356-023-29649-5","article-title":"A hybrid mesoporous composite of SnO2 and MgO for adsorption and photocatalytic degradation of anionic dye from a real industrial effluent water","volume":"30","author":"Naggar","year":"2023","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"e4741","DOI":"10.1002\/bio.4741","article-title":"Isoquinoline-based intrinsic fluorescence assessment of erythropoiesis-stimulating agent, Roxadustat (FG-4592), in tablets: Applications to content uniformity and human plasma evaluation","volume":"39","author":"Batakoushy","year":"2024","journal-title":"Luminescence"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"116223","DOI":"10.1016\/j.trac.2021.116223","article-title":"White Analytical Chemistry: An approach to reconcile the principles of Green Analytical Chemistry and functionality","volume":"138","author":"Nowak","year":"2021","journal-title":"TRAC Trends Anal. Chem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e4766","DOI":"10.1002\/bio.4766","article-title":"Design of resonance Rayleigh scattering and spectrofluorimetric methods for the determination of the antihistaminic drug, hydroxyzine, based on its interaction with 2, 4, 5, 7-tetraiodofluorescein: Evaluation of analytical eco-scale and greenness\/whiteness algorithms","volume":"39","author":"Darwish","year":"2024","journal-title":"Luminescence"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"10928","DOI":"10.3390\/molecules200610928","article-title":"Green chemistry metrics with special reference to green analytical chemistry","volume":"20","author":"Tobiszewski","year":"2015","journal-title":"Molecules"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"123046","DOI":"10.1016\/j.talanta.2021.123046","article-title":"Green analytical chemistry metrics: A review","volume":"238","author":"Sajid","year":"2022","journal-title":"Talanta"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"120848","DOI":"10.1016\/j.ces.2024.120848","article-title":"Suppressing vacancies and crystal water of sodium manganese iron-based Prussian blue analogue by potassium doping for advanced sodium-ion batteries","volume":"302","author":"Xu","year":"2025","journal-title":"Chem. Eng. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2204","DOI":"10.1007\/s10854-023-11606-0","article-title":"Synthesis of prussian blue analog (Co\/TCNi\/HCCr) nanoparticles using a facile co-precipitation approach and evaluation of their dielectric characteristics for electronic applications","volume":"34","author":"Mosaad","year":"2023","journal-title":"J. Mater. Sci. Mater. Electron."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"075004","DOI":"10.1088\/2053-1591\/aba0ea","article-title":"Prussian blue analogue cobalt tetracyanonickelate hexacyanochromate decorated by CNTs: Structural, morphological, optical characterization","volume":"7","author":"Elgazzar","year":"2020","journal-title":"Mater. Res. Express"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.ijhydene.2023.08.020","article-title":"Engineered Ni\u2013Fe prussian blue analogue nanocubes and their transformation into nanocages and mixed oxide for applications as bifunctional electrocatalyst","volume":"50","author":"Pal","year":"2024","journal-title":"Int. J. Hydrogen Energy"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2008989","DOI":"10.1002\/adfm.202008989","article-title":"Pd ion-exchange and ammonia etching of a prussian blue analogue to produce a high-performance water-splitting catalyst","volume":"31","author":"Zhang","year":"2021","journal-title":"Adv. Funct. Mater."},{"key":"ref_27","first-page":"290","article-title":"Insecticidal effect of zinc oxide and aluminum oxide nanoparticles synthesized by co-precipitation technique on Culex quinquefasciatus larvae (Diptera: Culicidae)","volume":"4","author":"Mostafa","year":"2018","journal-title":"Int. J. Appl. Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"124586","DOI":"10.1016\/j.colsurfa.2020.124586","article-title":"Synthesis, structural, optical and magnetic properties of NiFe2O4\/MWCNTs\/ZnO hybrid nanocomposite for solar radiation driven photocatalytic degradation and magnetic separation","volume":"592","author":"Hezam","year":"2020","journal-title":"Colloids Surf. A Physicochem. Eng. Asp."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/j.jcis.2024.07.211","article-title":"Self-assembled high-entropy Prussian blue analogue nanosheets enabling efficient sodium storage","volume":"677","author":"Gu","year":"2025","journal-title":"J. Colloid Interface Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.ijhydene.2024.09.155","article-title":"Fe-doped phosphide nanosheet array derived from prussian blue analogues for high-efficient electrocatalytic water splitting","volume":"88","author":"Wang","year":"2024","journal-title":"Int. J. Hydrogen Energy"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1016\/j.jcis.2022.02.007","article-title":"Mo, Fe-codoped metal phosphide nanosheets derived from Prussian blue analogues for efficient overall water splitting","volume":"615","author":"An","year":"2022","journal-title":"J. Colloid Interface Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"6721","DOI":"10.1016\/j.ijhydene.2021.12.059","article-title":"Preparation of graphite carbon\/Prussian blue analogue\/palladium (GC\/PBA\/pd) synergistic-effect electrocatalyst with high activity for ethanol oxidation reaction","volume":"47","author":"Yu","year":"2022","journal-title":"Int. J. Hydrogen Energy"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.ijleo.2016.10.057","article-title":"Preparation and characterization of spray-deposited efficient Prussian blue electrochromic thin film","volume":"129","author":"Elshorbagy","year":"2017","journal-title":"Optik"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Padhan, S., Wagri, N.K., Dash, L., Das, A., Das, S.R., Rafighi, M., and Sharma, P. (2023). Investigation on Surface Integrity in Hard Turning of AISI 4140 Steel with SPPP-AlTiSiN Coated Carbide Insert under Nano-MQL. Lubricants, 11.","DOI":"10.3390\/lubricants11020049"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Moumen, A., Kaur, N., Poli, N., Zappa, D., and Comini, E. (2020). One Dimensional ZnO Nanostructures: Growth and Chemical Sensing Performances. Nanomaterials, 10.","DOI":"10.3390\/nano10101940"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Rurack, K. (2008). Fluorescence quantum yields: Methods of determination and standards. Stand. Qual. Assur. Fluoresc. Meas. I Tech., 101\u2013145.","DOI":"10.1007\/4243_2008_019"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"343175","DOI":"10.1016\/j.aca.2024.343175","article-title":"Copper and nitrogen-doped carbon quantum dots as green nano-probes for fluorimetric determination of delafloxacin; characterization and applications","volume":"1327","author":"Salman","year":"2024","journal-title":"Anal. Chim. Acta"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"128014","DOI":"10.1016\/j.talanta.2025.128014","article-title":"Comprehensive Investigation of Prunus armeniaca for Natural Green Synthesis of Carbon Quantum Dots; Applications as Fluorescent Nano-probes for Ramipril","volume":"292","author":"Salman","year":"2025","journal-title":"Talanta"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1039\/D3LP00195D","article-title":"Dynamic quenching mechanism based optical detection of carcinogenic Cr(vi) in water and on economical paper test strips via a conjugated polymer","volume":"2","author":"Tanwar","year":"2024","journal-title":"RSC Appl. Polym."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2401957","DOI":"10.1002\/smll.202401957","article-title":"Prussian Blue Analogues for Sodium-Ion Battery Cathodes: A Review of Mechanistic Insights, Current Challenges, and Future Pathways","volume":"20","author":"Xiao","year":"2024","journal-title":"Small"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"112125","DOI":"10.1016\/j.microc.2024.112125","article-title":"Feasible synthesis and physicochemical features of a luminescent cadmium-metal organic frameworks (Cd-MOFs) composite, and its functionalization as a turn-off sensor towards selective determination of bisphenol A in food, water, and paper products","volume":"207","author":"Rashdan","year":"2024","journal-title":"Microchem. J."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Kumar Panigrahi, S., and Kumar Mishra, A. (2019). Inner filter effect in fluorescence spectroscopy: As a problem and as a solution. J. Photochem. Photobiol. C Photochem. Rev., 41.","DOI":"10.1016\/j.jphotochemrev.2019.100318"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"798","DOI":"10.1016\/j.jpba.2005.02.037","article-title":"Guidelines from the international conference on harmonisation (ICH)","volume":"38","author":"Branch","year":"2005","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"e20884","DOI":"10.1590\/s2175-97902022e20884","article-title":"Determination of sunset yellow, allura red, and fast green using a novel magnetic nanoadsorbent modified with Elaeagnus angustifolia based on magnetic solid-phase extraction by HPLC","volume":"58","author":"Oymak","year":"2022","journal-title":"Braz. J. Pharm. Sci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"142619","DOI":"10.1016\/j.foodchem.2024.142619","article-title":"Application of chemometrics based on digital image analysis for simultaneous determination of tartrazine and sunset yellow in food samples","volume":"470","author":"Hosseini","year":"2025","journal-title":"Food Chem."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2100207","DOI":"10.1002\/ppsc.202100207","article-title":"Fluorescence sensing performance of carbon dots of functionalization toward sunset yellow","volume":"39","author":"Xu","year":"2022","journal-title":"Part. Part. Syst. Charact."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"10343","DOI":"10.1021\/acs.analchem.9b01872","article-title":"What color is your method? Adaptation of the RGB additive color model to analytical method evaluation","volume":"91","author":"Nowak","year":"2019","journal-title":"Anal. Chem."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"10076","DOI":"10.1021\/acs.analchem.0c01887","article-title":"AGREE\u2014Analytical GREEnness Metric Approach and Software","volume":"92","author":"Wojnowski","year":"2020","journal-title":"Anal. Chem."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"116553","DOI":"10.1016\/j.trac.2022.116553","article-title":"AGREEprep\u2013analytical greenness metric for sample preparation","volume":"149","author":"Wojnowski","year":"2022","journal-title":"TrAC Trends Anal. Chem."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"451","DOI":"10.3390\/analytica5030030","article-title":"Modified GAPI (MoGAPI) tool and software for the assessment of method greenness: Case studies and applications","volume":"5","author":"Mansour","year":"2024","journal-title":"Analytica"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"e70063","DOI":"10.1002\/jssc.70063","article-title":"Leveraging Green Chromatography in Quality-by-Design-Driven Study for the Simultaneous Analysis of Essential Single-Pill Antidiabetic Drug Combinations","volume":"47","author":"Ibrahim","year":"2024","journal-title":"J. Sep. Sci."}],"container-title":["Biosensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6374\/15\/4\/263\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:18:23Z","timestamp":1760030303000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6374\/15\/4\/263"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,20]]},"references-count":51,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2025,4]]}},"alternative-id":["bios15040263"],"URL":"https:\/\/doi.org\/10.3390\/bios15040263","relation":{},"ISSN":["2079-6374"],"issn-type":[{"value":"2079-6374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,20]]}}}