{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T01:28:26Z","timestamp":1792978106956,"version":"4.5.0"},"reference-count":66,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T00:00:00Z","timestamp":1747785600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Zhejiang Provincial Natural Science Foundation of China","award":["LMS25B050001"],"award-info":[{"award-number":["LMS25B050001"]}]},{"name":"Zhejiang Provincial Natural Science Foundation of China","award":["2024GXNSFBA010303"],"award-info":[{"award-number":["2024GXNSFBA010303"]}]},{"name":"Joint Project on the Regional High-Incidence Diseases Research of Guangxi Natural Science Foundation","award":["LMS25B050001"],"award-info":[{"award-number":["LMS25B050001"]}]},{"name":"Joint Project on the Regional High-Incidence Diseases Research of Guangxi Natural Science Foundation","award":["2024GXNSFBA010303"],"award-info":[{"award-number":["2024GXNSFBA010303"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>Glucose (Glu) detection, as a fundamental analytical technique, has applications in medical diagnostics, clinical testing, bioanalysis and environmental monitoring. In this work, a solid-phase electrochemiluminescence (ECL) enzyme sensor was developed by immobilizing the ECL emitter in a stable manner within bipolar silica nanochannel array film (bp-SNA), enabling sensitive glucose detection. The sensor was constructed using an electrochemical-assisted self-assembly (EASA) method with various siloxane precursors to quickly modify the surface of indium tin oxide (ITO) electrodes with a bilayer SNA of different charge properties. The inner layer, including negatively charged SNA (n-SNA), attracted the positively charged ECL emitter tris(2,2\u2032-bipyridyl)ruthenium(II) (Ru(bpy)32+) via electrostatic interaction, while the outer layer, including positively charged SNA (p-SNA), repelled it, forming a barrier that efficiently concentrated the Ru(bpy)32+ emitter in a stable manner. After modifying the amine groups on the p-SNA surface with aldehyde groups, glucose oxidase (GOx) was covalently immobilized, forming the enzyme electrode. In the presence of glucose, GOx catalyzed the conversion of glucose to hydrogen peroxide (H2O2), which acted as a quencher for the Ru(bpy)32+\/triethanolamine (TPA) system, reducing the ECL signal and enabling quantitative glucose analysis. The sensor exhibited a wide linear range from 10 \u03bcM to 7.0 mM and a limit of detection (LOD) of 1 \u03bcM (S\/N = 3). Glucose detection in fetal bovine serum was realized. By replacing the enzyme type on the electrode surface, this sensing strategy holds the potential to provide a universal platform for the detection of different metabolites.<\/jats:p>","DOI":"10.3390\/bios15050332","type":"journal-article","created":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T13:59:43Z","timestamp":1747835983000},"page":"332","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Enzyme-Based Solid-Phase Electrochemiluminescence Sensors with Stable, Anchored Emitters for Sensitive Glucose Detection"],"prefix":"10.3390","volume":"15","author":[{"given":"Chunyin","family":"Wei","sequence":"first","affiliation":[{"name":"Guangxi Medical University Cancer Hospital, Guangxi Medical University, Nanning 530021, China"},{"name":"Postdoctoral Research Center, School of Basic Medicine, Guangxi Medical University, Nanning 530021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanyan","family":"Zheng","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Zhejiang Sci-Tech University, Hangzhou 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2822-698X","authenticated-orcid":false,"given":"Fei","family":"Yan","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Zhejiang Sci-Tech University, Hangzhou 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lifang","family":"Xu","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital of Guangxi Medical University, Nanning 530007, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"114778","DOI":"10.1016\/j.sna.2023.114778","article-title":"Recent advances in electrochemical non-enzymatic glucose sensor for the detection of glucose in tears and saliva: A review","volume":"363","author":"Jarnda","year":"2023","journal-title":"Sens. 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