{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T08:34:21Z","timestamp":1789029261432,"version":"build-2803163510"},"reference-count":44,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2025,6,18]],"date-time":"2025-06-18T00:00:00Z","timestamp":1750204800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Aidmics Biotechnology"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>This study presents a mobile-based sperm analysis system featuring a user-friendly, droplet-loaded microfluidic chip that enables non-specialist users to perform the rapid and accurate quantitative evaluation of boar semen directly on the farm. The iSperm system integrates a tablet, optical module, heater, and real-time image analysis app to deliver automated measurements of sperm concentration, motility, and progressive motility in under one minute. Precision and user variability tests demonstrated high concordance with CASA and the hemocytometer, with minimal differences between trained and untrained users. A method comparison using 77 farm-collected samples confirmed agreement through Passing\u2013Bablok regression and Bland\u2013Altman analysis. ROC curve analyses further validated diagnostic accuracy for all parameters, with AUC values exceeding 0.95. The iSperm platform offers a reliable, user-friendly, and field-deployable solution for on-site semen quality assessment, improving decision-making in swine artificial insemination.<\/jats:p>","DOI":"10.3390\/bios15060394","type":"journal-article","created":{"date-parts":[[2025,6,19]],"date-time":"2025-06-19T05:08:39Z","timestamp":1750309719000},"page":"394","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A Mobile Sperm Analyzer with User-Friendly Microfluidic Chips for Rapid On-Farm Semen Evaluation"],"prefix":"10.3390","volume":"15","author":[{"given":"Shu-Sheng","family":"Lin","sequence":"first","affiliation":[{"name":"Institute of Applied Mechanics, National Taiwan University, Taipei 106319, Taiwan"},{"name":"Aidmics Biotechnology, Taipei 10647, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang-Yu","family":"Chen","sequence":"additional","affiliation":[{"name":"Aidmics Biotechnology, Taipei 10647, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng-Ming","family":"Lin","sequence":"additional","affiliation":[{"name":"Aidmics Biotechnology, Taipei 10647, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsun-Chao","family":"Chiang","sequence":"additional","affiliation":[{"name":"Aidmics Biotechnology, Taipei 10647, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Siang","family":"Tang","sequence":"additional","affiliation":[{"name":"Aidmics Biotechnology, Taipei 10647, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang-Ching","family":"Yeh","sequence":"additional","affiliation":[{"name":"Aidmics Biotechnology, Taipei 10647, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-Fan","family":"Hsu","sequence":"additional","affiliation":[{"name":"Institute of Applied Mechanics, National Taiwan University, Taipei 106319, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew M.","family":"Wo","sequence":"additional","affiliation":[{"name":"Institute of Applied Mechanics, National Taiwan University, Taipei 106319, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1002\/mrd.23566","article-title":"Science-based quality control in boar semen production","volume":"90","author":"Schulze","year":"2023","journal-title":"Mol. 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