{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T15:07:55Z","timestamp":1785337675125,"version":"3.55.0"},"reference-count":36,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2025,8,27]],"date-time":"2025-08-27T00:00:00Z","timestamp":1756252800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"\u201cEnsuring chemical and biological safety of the Russian Federation\u201d"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>Enteric viruses are a major cause of waterborne infections due to their high environmental stability and extremely low infectious dose. Current molecular diagnostic methods, while accurate, often depend on thermal cycling and centralized laboratory facilities, limiting their applicability in decentralized or resource-limited settings. In this study, we developed an isothermal biosensor based on a split deoxyribozyme that reconstitutes its catalytic core upon hybridization with a conserved sequence of enteroviral RNA. This activation leads to site-specific cleavage of a fluorogenic substrate, producing a quantifiable fluorescent signal. The system was experimentally validated using both synthetic enteroviral RNA and RNA extracted from environmental water samples. To enhance detection sensitivity, the DNAzyme-based assay was coupled with isothermal RNA amplification. The results demonstrate high selectivity and compatibility with real-world samples, supporting the sensor\u2019s utility for field-deployable viral RNA detection. Overall, this study highlights the potential of the DNAzyme-based platform as a portable, sequence-specific, and amplification-assisted diagnostic tool for environmental surveillance of enteric viruses.<\/jats:p>","DOI":"10.3390\/bios15090562","type":"journal-article","created":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T09:31:12Z","timestamp":1756373472000},"page":"562","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["An Isothermal Deoxyribozyme Sensor for Rapid Detection of Enteroviral RNA"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-4831-6382","authenticated-orcid":false,"given":"Beg\u00fcm","family":"\u015ea\u015f","sequence":"first","affiliation":[{"name":"Laboratory of Pathogen Molecular Genetics, Saint Petersburg Pasteur Institute, 197101 St. Petersburg, Russia"},{"name":"Faculty of Biotechnologies, ITMO University, 191002 St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9659-5189","authenticated-orcid":false,"given":"Anastasiia Dmitrievna","family":"Kirichenko","sequence":"additional","affiliation":[{"name":"Laboratory of Pathogen Molecular Genetics, Saint Petersburg Pasteur Institute, 197101 St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3322-2032","authenticated-orcid":false,"given":"Marina Anatolyevna","family":"Kapitonova","sequence":"additional","affiliation":[{"name":"Laboratory of Pathogen Molecular Genetics, Saint Petersburg Pasteur Institute, 197101 St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna Vyacheslavovna","family":"Shabalina","sequence":"additional","affiliation":[{"name":"Laboratory of Pathogen Molecular Genetics, Saint Petersburg Pasteur Institute, 197101 St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7699-5097","authenticated-orcid":false,"given":"Olga Ilyinichna","family":"Kanaeva","sequence":"additional","affiliation":[{"name":"Laboratory of Etiology and Control of Viral Infections, Saint Petersburg Pasteur Institute, 197101 St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4824-5774","authenticated-orcid":false,"given":"Tamer Mohammed","family":"El-Messery","sequence":"additional","affiliation":[{"name":"Faculty of Biotechnologies, ITMO University, 191002 St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5500-0169","authenticated-orcid":false,"given":"Vladimir Georgievich","family":"Dedkov","sequence":"additional","affiliation":[{"name":"Laboratory of Pathogen Molecular Genetics, Saint Petersburg Pasteur Institute, 197101 St. Petersburg, Russia"},{"name":"Martsinovsky Institute of Medical Parasitology, Tropical and Vector Borne Diseases, Sechenov First Moscow State Medical University (Sechenov University), 119048 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8730-4872","authenticated-orcid":false,"given":"Anna Sergeevna","family":"Dolgova","sequence":"additional","affiliation":[{"name":"Laboratory of Pathogen Molecular Genetics, Saint Petersburg Pasteur Institute, 197101 St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Olaimat, A.N., Taybeh, A.O., Al-Nabulsi, A., Al-Holy, M., Hatmal, M.M., Alzyoud, J., Aolymat, I., Abughoush, M.H., Shahbaz, H., and Alzyoud, A. 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