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In this study, a novel chemical sensor based on the laccase-like activity of CH3COOAg was developed for the selective detection of AGs. CH3COOAg exhibited varying degrees of laccase-like activity in different buffers (MES, HEPES, and NaAc) and H2O, and five AGs showed distinct intensities of the inhibitory effect on the laccase-like activity of CH3COOA in different buffers and H2O. Therefore, a four-channel colorimetric sensor array was constructed in combination with the use of principal component analysis (PCA) and Hierarchical Cluster Analysis (HCA) for the efficient identification of five AGs (0.02\u20130.3 \u03bcM) in environment samples like tap and lake water. In addition, a colorimetric method was developed for kanamycin (KAN) detection in a honey sample with a linear range of 10\u2013100 nM (R2 = 0.9977). The method has excellent sensitivity with a limit of detection of 3.99 nM for KAN. This work not only provides a rapid and low-cost detection method for AG monitoring but also provides a reference for the design of non-copper laccase mimics.<\/jats:p>","DOI":"10.3390\/bios15090570","type":"journal-article","created":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T12:04:28Z","timestamp":1756814668000},"page":"570","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["CH3COOAg with Laccase-like Activity for Differentiation and Detection of Aminoglycoside Antibiotics"],"prefix":"10.3390","volume":"15","author":[{"given":"Huan","family":"Zhu","sequence":"first","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tong-Qing","family":"Chai","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia-Xin","family":"Li","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing-Jing","family":"Dai","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Ling","family":"Qin","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Natural Product Synthesis and Drug Research, School of Pharmaceutical Sciences, Chongqing University, Chongqing 401331, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9300-5710","authenticated-orcid":false,"given":"Feng-Qing","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 401331, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Nowacka-Kozak, E., Gajda, A., and Gbylik-Sikorska, M. 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