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With human adenovirus type 5 (HAdV-5) particles as the target, we here demonstrate the application of the aptamer\/FET combination for detection of this medically and biotechnologically relevant viral vector. A focused anti-HAdV-5 aptamer library was evolved in a nine-round SELEX process, allowing for the specific fluorescent labeling of HAdV-5 and related subtypes. Moreover, this library was already sufficient to serve as the binding entity on a gFET sensor for sensitive quantification of the virus particles. Adenoviruses have been widely used as gene delivery vectors for gene therapy and genetic vaccination. The use of adenoviral vectors within the vaccination campaign against COVID-19 emphasized the need for robust biotechnological production processes, which additionally require sensitive product formation monitoring. We believe that these type of gFET-based aptasensors can serve as the technological monitoring basis in virus production processes in the near future.<\/jats:p>","DOI":"10.3390\/bios15090605","type":"journal-article","created":{"date-parts":[[2025,9,15]],"date-time":"2025-09-15T11:51:43Z","timestamp":1757937103000},"page":"605","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["An Aptamer-Based gFET-Sensor for Specific Quantification of Gene Therapeutic Human Adenovirus Type 5"],"prefix":"10.3390","volume":"15","author":[{"given":"Runliu","family":"Li","sequence":"first","affiliation":[{"name":"Institute of Pharmaceutical Biotechnology, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7164-0166","authenticated-orcid":false,"given":"Ann-Kathrin","family":"Kissmann","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Biotechnology, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hu","family":"Xing","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Biotechnology, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0883-3053","authenticated-orcid":false,"given":"Roger","family":"Hasler","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and Dentistry, Danube Private University, Steiner Landstra\u00dfe 124, 3500 Krems an der Donau, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1868-4960","authenticated-orcid":false,"given":"Christoph","family":"Kleber","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and Dentistry, Danube Private University, Steiner Landstra\u00dfe 124, 3500 Krems an der Donau, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang","family":"Knoll","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and Dentistry, Danube Private University, Steiner Landstra\u00dfe 124, 3500 Krems an der Donau, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannes","family":"Schmietendorf","sequence":"additional","affiliation":[{"name":"Department of Gene Therapy, Ulm University, Helmholtzstra\u00dfe 8\/1, 89081 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatjana","family":"Engler","sequence":"additional","affiliation":[{"name":"Department of Gene Therapy, Ulm University, Helmholtzstra\u00dfe 8\/1, 89081 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lea","family":"Krutzke","sequence":"additional","affiliation":[{"name":"Department of Gene Therapy, Ulm University, Helmholtzstra\u00dfe 8\/1, 89081 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Kochanek","sequence":"additional","affiliation":[{"name":"Department of Gene Therapy, Ulm University, Helmholtzstra\u00dfe 8\/1, 89081 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9297-6419","authenticated-orcid":false,"given":"Frank","family":"Rosenau","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Biotechnology, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1038\/346818a0","article-title":"In Vitro Selection of RNA Molecules That Bind Specific Ligands","volume":"346","author":"Ellington","year":"1990","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1126\/science.2200121","article-title":"Systematic Evolution of Ligands by Exponential Enrichment: RNA Ligands to Bacteriophage T4 DNA Polymerase","volume":"249","author":"Tuerk","year":"1990","journal-title":"Science"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"16281","DOI":"10.3390\/s150716281","article-title":"Nucleic Acid Aptamers: An Emerging Tool for Biotechnology and Biomedical Sensing","volume":"15","author":"Ku","year":"2015","journal-title":"Sensors"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1551","DOI":"10.1039\/C5AN01824B","article-title":"Aptamers in Analytics","volume":"141","author":"Ilgu","year":"2016","journal-title":"Analyst"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1038\/nrmicro1458","article-title":"Aptamers Come of Age\u2014At Last","volume":"4","author":"Bunka","year":"2006","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_6","unstructured":"Chopra, A., Shukla, R., and Sharma, T.K. (2014). Aptamers as an Emerging Player in Biology. Front. Genet., 5."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1016\/j.bios.2015.04.041","article-title":"Replacing Antibodies with Aptamers in Lateral Flow Immunoassay","volume":"71","author":"Chen","year":"2015","journal-title":"Biosens. Bioelectron."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Guo, W., Zhang, C., Ma, T., Liu, X., Chen, Z., Li, S., and Deng, Y. (2021). Advances in Aptamer Screening and Aptasensors\u2019 Detection of Heavy Metal Ions. J. Nanobiotechnol., 19.","DOI":"10.1186\/s12951-021-00914-4"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Kusumawati, A., Mustopa, A.Z., Wibawan, I.W.T., Setiyono, A., and Sudarwanto, M.B. (2022). A Sequential Toggle Cell-SELEX DNA Aptamer for Targeting Staphylococcus Aureus, Streptococcus Agalactiae, and Escherichia Coli Bacteria. J. Genet. Eng. Biotechnol., 20.","DOI":"10.1186\/s43141-022-00374-9"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"7369","DOI":"10.1021\/acs.analchem.1c01445","article-title":"Identification of a New DNA Aptamer by Tissue-SELEX for Cancer Recognition and Imaging","volume":"93","author":"Li","year":"2021","journal-title":"Anal. Chem."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Murakami, K., Izuo, N., and Bitan, G. (2022). Aptamers Targeting Amyloidogenic Proteins and Their Emerging Role in Neurodegenerative Diseases. J. Biol. Chem., 298.","DOI":"10.1016\/j.jbc.2021.101478"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1038\/nprot.2010.66","article-title":"Development of DNA Aptamers Using Cell-SELEX","volume":"5","author":"Sefah","year":"2010","journal-title":"Nat. Protoc."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1525","DOI":"10.1021\/ac302721j","article-title":"Capillary Electrophoresis-SELEX Selection of Catalytic DNA Aptamers for a Small-Molecule Porphyrin Target","volume":"85","author":"Yang","year":"2013","journal-title":"Anal. Chem."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"8289","DOI":"10.1021\/acs.analchem.9b01000","article-title":"Identification and Application of an Aptamer Targeting Papillary Thyroid Carcinoma Using Tissue-SELEX","volume":"91","author":"Zhong","year":"2019","journal-title":"Anal. Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1080\/10408347.2016.1157014","article-title":"SELEX Modifications and Bioanalytical Techniques for Aptamer\u2013Target Binding Characterization","volume":"46","author":"Tan","year":"2016","journal-title":"Crit. Rev. Anal. Chem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1007\/s00216-005-3388-9","article-title":"FluMag-SELEX as an Advantageous Method for DNA Aptamer Selection","volume":"383","author":"Stoltenburg","year":"2005","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"14536","DOI":"10.1002\/chem.202000213","article-title":"The Diversity of a Polyclonal FluCell-SELEX Library Outperforms Individual Aptamers as Emerging Diagnostic Tools for the Identification of Carbapenem Resistant Pseudomonas Aeruginosa","volume":"26","author":"Kubiczek","year":"2020","journal-title":"Chemistry"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Raber, H.F., Kubiczek, D.H., Bodenberger, N., Kissmann, A.K., D\u2019souza, D., Hu, X., Mayer, D., Xu, P., Knippschild, U., and Spellerberg, B. (2021). FluCell-SELEX Aptamers as Specific Binding Molecules for Diagnostics of the Health Relevant Gut Bacterium Akkermansia Muciniphila. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms221910425"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Xing, H., Zhang, Y., Kr\u00e4mer, M., Kissmann, A.K., Henkel, M., Weil, T., Knippschild, U., and Rosenau, F. (2022). A Polyclonal Selex Aptamer Library Directly Allows Specific Labelling of the Human Gut Bacterium Blautia Producta without Isolating Individual Aptamers. Molecules, 27.","DOI":"10.3390\/molecules27175693"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Xing, H., Kissmann, A.K., Raber, H.F., Kr\u00e4mer, M., Amann, V., Kohn, K., Weil, T., and Rosenau, F. (2021). Polyclonal Aptamers for Specific Fluorescence Labeling and Quantification of the Health Relevant Human Gut Bacterium Parabacteroides Distasonis. Microorganisms, 9.","DOI":"10.3390\/microorganisms9112284"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Zhang, Y., Xing, H., Bolotnikov, G., Kr\u00e4mer, M., Gotzmann, N., Knippschild, U., Kissmann, A.K., and Rosenau, F. (2023). Enriched Aptamer Libraries in Fluorescence-Based Assays for Rikenella Microfusus-Specific Gut Microbiome Analyses. Microorganisms, 11.","DOI":"10.20944\/preprints202307.2103.v1"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Xing, H., Zhang, Y., Kr\u00e4mer, M., Kissmann, A.K., Amann, V., Raber, H.F., Weil, T., Stieger, K.R., Knippschild, U., and Henkel, M. (2022). A Polyclonal Aptamer Library for the Specific Binding of the Gut Bacterium Roseburia Intestinalis in Mixtures with Other Gut Microbiome Bacteria and Human Stool Samples. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23147744"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Knei\u00dfle, K., Kr\u00e4mer, M., Kissmann, A.K., Xing, H., M\u00fcller, F., Amann, V., Noschka, R., Gottschalk, K.E., Bozdogan, A., and Andersson, J. (2022). A Polyclonal SELEX Aptamer Library Allows Differentiation of Candida albicans, C. auris and C. parapsilosis Cells from Human Dermal Fibroblasts. J. Fungi, 8.","DOI":"10.3390\/jof8080856"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Zhang, Y., Xing, H., Bolotnikov, G., Kr\u00e4mer, M., Bozdogan, A., Kissmann, A.K., Weil, T., Spellerberg, B., Stenger, S., and Rosenau, F. (2024). Robust Fluorometric Aptamer Assay for Direct and Rapid Detection of Clinical Isolates of Candida Spec. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25063444"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Kissmann, A.K., Wolf, D., Kr\u00e4mer, M., M\u00fcller, F., Amann, V., Xing, H., Gottschalk, K.E., Weil, T., Eichmann, R., and Sch\u00e4fer, P. (2022). Polyclonal Aptamer Libraries from a FluRoot-SELEX for the Specific Labeling of the Apical and Elongation\/Differentiation Zones of Arabidopsis Thaliana Roots. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms232012220"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"36","DOI":"10.3390\/biomedicines2010036","article-title":"Oncolytic Adenoviruses in Cancer Treatment","volume":"2","author":"Alemany","year":"2014","journal-title":"Biomedicines"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2979","DOI":"10.1158\/1078-0432.CCR-20-1565","article-title":"Evolving Status of Clinical Immunotherapy with Oncolytic Adenovirus","volume":"27","author":"Alemany","year":"2021","journal-title":"Clin. Cancer Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"114305","DOI":"10.1016\/j.jviromet.2021.114305","article-title":"Rapid and Inexpensive Purification of Adenovirus Vectors Using an Optimised Aqueous Two-Phase Technology","volume":"299","author":"Suleman","year":"2022","journal-title":"J. Virol. Methods"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Dicks, M.D.J., Spencer, A.J., Edwards, N.J., Wadell, G., Bojang, K., Gilbert, S.C., Hill, A.V.S., and Cottingham, M.G. (2012). A Novel Chimpanzee Adenovirus Vector with Low Human Seroprevalence: Improved Systems for Vector Derivation and Comparative Immunogenicity. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0040385"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1038\/s41541-020-00243-x","article-title":"Ad26 Vector-Based COVID-19 Vaccine Encoding a Prefusion-Stabilized SARS-CoV-2 Spike Immunogen Induces Potent Humoral and Cellular Immune Responses","volume":"5","author":"Bos","year":"2020","journal-title":"NPJ Vaccines"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"4081","DOI":"10.1038\/s41467-020-17972-1","article-title":"A Single Dose of an Adenovirus-Vectored Vaccine Provides Protection against SARS-CoV-2 Challenge","volume":"11","author":"Wu","year":"2020","journal-title":"Nat. Commun."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Pilely, K., Johansen, M.R., Lund, R.R., Kofoed, T., J\u00f8rgensen, T.K., Skriver, L., and M\u00f8rtz, E. (2021). Monitoring Process-Related Impurities in Biologics\u2013Host Cell Protein Analysis. Anal. Bioanal. Chem., 414.","DOI":"10.1007\/s00216-021-03648-2"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1210","DOI":"10.1002\/jmv.20683","article-title":"Molecular Identification of Adenovirus Sequences: A Rapid Scheme for Early Typing of Human Adenoviruses in Diagnostic Samples of Immunocompetent and Immunodeficient Patients","volume":"78","author":"Madisch","year":"2006","journal-title":"J. Med. Virol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1002\/jmv.10382","article-title":"Rapid and Quantitative Detection of Human Adenovirus DNA by Real-Time PCR","volume":"70","author":"Heim","year":"2003","journal-title":"J. Med. Virol"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1089\/genbio.2024.0043","article-title":"Real-Time Monitoring in Biomanufacturing with Graphene Field-Effect Transistor Sensors: Detection of PH, Glucose, and Antibodies","volume":"3","author":"Ban","year":"2024","journal-title":"GEN Biotechnol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"116801","DOI":"10.1016\/j.foodres.2025.116801","article-title":"Sensor Detection of Shelf Life: A Multi-Technique Food Analytical Platform for Mushroom Analysis","volume":"217","author":"Saiki","year":"2025","journal-title":"Food Res. Int."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"e2407487","DOI":"10.1002\/adma.202407487","article-title":"Ultrasensitive Detection of Chemokines in Clinical Samples with Graphene-Based Field-Effect Transistors","volume":"36","author":"Kaiser","year":"2024","journal-title":"Adv. Mater."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"4622","DOI":"10.1021\/acsabm.4c00484","article-title":"Graphene Multiplexed Sensor for Point-of-Need Viral Wastewater-Based Epidemiology","volume":"7","author":"Geiwitz","year":"2024","journal-title":"ACS Appl. Bio Mater."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2300324","DOI":"10.1002\/pssb.202300324","article-title":"Liquid-Gated Graphene Field-Effect Transistors for Biosensing on Lipid Monolayers","volume":"260","author":"Fomin","year":"2023","journal-title":"Phys. Status Solidi B Basic Res."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Selvarajan, R.S., Rahim, R.A., Majlis, B.Y., Gopinath, S.C.B., and Hamzah, A.A. (2020). Ultrasensitive and Highly Selective Graphene-Based Field-Effect Transistor Biosensor for Anti-Diuretic Hormone Detection. Sensors, 20.","DOI":"10.3390\/s20092642"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"128432","DOI":"10.1016\/j.snb.2020.128432","article-title":"Graphene Based Field-Effect Transistor Biosensors Functionalized Using Gas-Phase Synthesized Gold Nanoparticles","volume":"320","author":"Danielson","year":"2020","journal-title":"Sens. Actuators B Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1039\/D0AN01661F","article-title":"Graphene Field-Effect Transistors as Bioanalytical Sensors: Design, Operation and Performance","volume":"146","author":"Sauvage","year":"2021","journal-title":"Analyst"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.bios.2015.03.013","article-title":"Graphene-Based Liquid-Gated Field Effect Transistor for Biosensing: Theory and Experiments","volume":"70","author":"Larisika","year":"2015","journal-title":"Biosens. Bioelectron."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Wang, G., Zhang, M., Zhu, M., Zhang, T., Qian, X., Liu, Y., Ma, X., Dai, C., Wei, D., and Zhu, Z. (2025). Ultraprecise Detection of Influenza Virus by Antibody-Modified Graphene Transistors. Sensors, 25.","DOI":"10.3390\/s25030959"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1909","DOI":"10.1021\/acssensors.4c03049","article-title":"Ultrasensitive, Real-Time Detection of Viral Antigens and RNA Enabled by Scalable Graphene-Based FET Sensors for Pathogen Detection: A Case Study on COVID-19","volume":"10","author":"Luo","year":"2025","journal-title":"ACS Sens."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"770","DOI":"10.1039\/D1NH00605C","article-title":"Polyclonal Aptamer Libraries as Binding Entities on a Graphene FET Based Biosensor for the Discrimination of Apo- and Holo-Retinol Binding Protein 4","volume":"7","author":"Kissmann","year":"2022","journal-title":"Nanoscale Horiz."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"4004","DOI":"10.1021\/acs.nanolett.9b01335","article-title":"Electrical Biosensing at Physiological Ionic Strength Using Graphene Field-Effect Transistor in Femtoliter Microdroplet","volume":"19","author":"Ono","year":"2019","journal-title":"Nano Lett."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Wang, S., Hossain, M.Z., Shinozuka, K., Shimizu, N., Kitada, S., Suzuki, T., Ichige, R., Kuwana, A., and Kobayashi, H. (2020). Graphene Field-Effect Transistor Biosensor for Detection of Biotin with Ultrahigh Sensitivity and Specificity. Biosens. Bioelectron., 165.","DOI":"10.1016\/j.bios.2020.112363"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Vu, C.-A., and Chen, W.-Y. (2020). Predicting Future Prospects of Aptamers in Field-Effect Transistor Biosensors. Molecules, 25.","DOI":"10.3390\/molecules25030680"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Shaban, S.M., and Kim, D.-H. (2021). Recent Advances in Aptamer Sensors. Sensors, 21.","DOI":"10.3390\/s21030979"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"S\u00e1nchez-Tirado, E., Ag\u00fc\u00ed, L., Gonz\u00e1lez-Cort\u00e9s, A., Campuzano, S., Y\u00e1\u00f1ez-Sede\u00f1o, P., and Pingarr\u00f3n, J.M. (2023). Electrochemical (Bio)Sensing Devices for Human-Microbiome-Related Biomarkers. Sensors, 23.","DOI":"10.3390\/s23020837"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Kissmann, A.K., Bolotnikov, G., Li, R., M\u00fcller, F., Xing, H., Kr\u00e4mer, M., Gottschalk, K.E., Andersson, J., Weil, T., and Rosenau, F. (2024). IMPATIENT-QPCR: Monitoring SELEX Success during in Vitro Aptamer Evolution. Appl. Microbiol. Biotechnol., 108.","DOI":"10.1007\/s00253-024-13085-7"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Herdina, A.N., Bozdogan, A., Aspermair, P., Dostalek, J., Klausberger, M., Lingg, N., Cserjan-Puschmann, M., Aguilar, P.P., Auer, S., and Demirtas, H. (2025). Bridging Basic Science and Applied Diagnostics: Comprehensive Viral Diagnostics Enabled by Graphene-Based Electronic Biosensor Technology Advancements. Biosens. Bioelectron., 267.","DOI":"10.1016\/j.bios.2024.116807"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1049\/mna2.12070","article-title":"The Top Performer: Towards Optimized Parameters for Reduced Graphene Oxide Uniformity by Spin Coating","volume":"16","author":"Hasler","year":"2021","journal-title":"Micro Nano Lett."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Leikas, A.J., Yl\u00e4-Herttuala, S., and Hartikainen, J.E.K. (2023). Adenoviral Gene Therapy Vectors in Clinical Use\u2014Basic Aspects with a Special Reference to Replication-Competent Adenovirus Formation and Its Impact on Clinical Safety. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms242216519"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"947","DOI":"10.3324\/haematol.2021.280154","article-title":"Comparative Analysis of ChAdOx1 NCoV-19 and Ad26.COV2.S SARS-CoV-2 Vector Vaccines","volume":"107","author":"Michalik","year":"2022","journal-title":"Haematologica"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1039\/D4NH00281D","article-title":"A Blautia Producta Specific GFET-Based Aptasensor for Quantitative Monitoring of Microbiome Quality","volume":"10","author":"Xing","year":"2024","journal-title":"Nanoscale Horiz."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"e2403827","DOI":"10.1002\/adhm.202403827","article-title":"Specific GFET-Based Aptasensors for Monitoring of Microbiome Quality: Quantification of the Enteric Health-Relevant Bacterium Roseburia Intestinalis","volume":"14","author":"Zhang","year":"2025","journal-title":"Adv. Heal. Mater."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"104522","DOI":"10.1016\/j.isci.2022.104522","article-title":"Ultrasensitive Detection of Exosomal MiRNA with PMO-Graphene Quantum Dots-Functionalized Field-Effect Transistor Biosensor","volume":"25","author":"Li","year":"2022","journal-title":"iScience"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.bios.2018.03.014","article-title":"Rapid Detection of Single E. Coli Bacteria Using a Graphene-Based Field-Effect Transistor Device","volume":"110","author":"Thakur","year":"2018","journal-title":"Biosens. Bioelectron."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1016\/j.bios.2016.09.025","article-title":"Label-Free Graphene Biosensor Targeting Cancer Molecules Based on Non-Covalent Modification","volume":"87","author":"Zhou","year":"2017","journal-title":"Biosens. Bioelectron."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Thriveni, G., and Ghosh, K. (2022). Advancement and Challenges of Biosensing Using Field Effect Transistors. Biosensors, 12.","DOI":"10.3390\/bios12080647"},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Forsyth, R., Devadoss, A., and Guy, O. (2017). Graphene Field Effect Transistors for Biomedical Applications: Current Status and Future Prospects. Diagnostics, 7.","DOI":"10.3390\/diagnostics7030045"},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Gallardo, J., P\u00e9rez-Illana, M., Mart\u00edn-Gonz\u00e1lez, N., and San Mart\u00edn, C. (2021). Adenovirus Structure: What Is New?. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22105240"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1016\/j.jbiotec.2013.10.024","article-title":"Aptamer Modification Improves the Adenoviral Transduction of Malignant Glioma Cells","volume":"168","author":"Chen","year":"2013","journal-title":"J. Biotechnol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"6830","DOI":"10.18632\/oncotarget.23564","article-title":"Characterization of Aptamer-Mediated Gene Delivery System for Liver Cancer Therapy","volume":"9","author":"Liu","year":"2017","journal-title":"Oncotarget"},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Kukushkin, V., Ambartsumyan, O., Subekin, A., Astrakhantseva, A., Gushchin, V., Nikonova, A., Dorofeeva, A., Zverev, V., Keshek, A., and Meshcheryakova, N. (2023). Multiplex Lithographic SERS Aptasensor for Detection of Several Respiratory Viruses in One Pot. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24098081"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"eabh2848","DOI":"10.1126\/sciadv.abh2848","article-title":"Direct Detection of Human Adenovirus or SARS-CoV-2 with Ability to Inform Infectivity Using DNA Aptamer-Nanopore Sensors","volume":"7","author":"Peinetti","year":"2021","journal-title":"Sci. Adv."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"2137","DOI":"10.1007\/s00216-023-04760-1","article-title":"Graphene-Based Field-Effect Transistors for Biosensing: Where Is the Field Heading To?","volume":"416","author":"Szunerits","year":"2024","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1126\/science.aao6750","article-title":"Aptamer\u2013Field-Effect Transistors Overcome Debye Length Limitations for Small-Molecule Sensing","volume":"362","author":"Nakatsuka","year":"2018","journal-title":"Science"},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"Sekhon, S., Bayford, R., and Demosthenous, A. (2025). Capacitive Sensors for Label-Free Detection in High-Ionic-Strength Bodily Fluids: A Review. Biosensors, 15.","DOI":"10.3390\/bios15080491"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"128375","DOI":"10.1016\/j.talanta.2025.128375","article-title":"The Optimal Ionic Concentration of Sensing Buffer in the Detection of RNA by Using the DNA Probe with the Silicon Nanowire Field-Effect Transistor (SiNW-FET)","volume":"295","author":"Hu","year":"2025","journal-title":"Talanta"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"6184","DOI":"10.1021\/acs.nanolett.5c00454","article-title":"Epitope-Imprinted Field-Effect Transistors Overcome Debye Length Limitations for Label-Free Protein Detection","volume":"25","author":"Wang","year":"2025","journal-title":"Nano Lett."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"101391","DOI":"10.1016\/j.nantod.2022.101391","article-title":"Highly Performing Graphene-Based Field Effect Transistor for the Differentiation between Mild-Moderate-Severe Myocardial Injury","volume":"43","author":"Rodrigues","year":"2022","journal-title":"Nano Today"},{"key":"ref_75","doi-asserted-by":"crossref","unstructured":"Xing, H., Li, R., Zhang, Y., Rajpal, S., Bolotnikov, G., Gruber, D., Ruzicka, H.-M., Andersson, J., Bozdogan, A., and Herdina, A.N. (2025, August 28). Single-Sequence Based GFET-Aptasensors for the Discrimination of Apo\u2013and Holo-RBP4 in Human Serum. Available online: https:\/\/ssrn.com\/abstract=5345344.","DOI":"10.2139\/ssrn.5345344"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1086\/341770","article-title":"High Levels of Adenovirus DNA in Serum Correlate with Fatal Outcome of Adenovirus Infection in Children after Allogeneic Stem-Cell Transplantation","volume":"35","author":"Schilham","year":"2002","journal-title":"Clin. Infect. Dis."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"2000","DOI":"10.1128\/JVI.80.4.2000-2012.2006","article-title":"Genetic Identification of Adenovirus Type 5 Genes That Influence Viral Spread","volume":"80","author":"Subramanian","year":"2006","journal-title":"J. Virol."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1016\/j.jviromet.2016.09.002","article-title":"The Influence of Incubation Time on Adenovirus Quantitation in A549 Cells by Most Probable Number","volume":"237","author":"Cashdollar","year":"2016","journal-title":"J. Virol. Methods"},{"key":"ref_79","doi-asserted-by":"crossref","unstructured":"Li, X., Zhao, C., Hou, G., Sun, Z., Liu, X., Ding, Y., Fang, Y., and Liu, Q. (2025). Simultaneously Ultrasensitive and Differential Detection of SARS-CoV-2, Adenovirus and Influenza a Virus Using Multiplex Fluorescence Lateral Flow Immunoassay. Front. Immunol., 16.","DOI":"10.3389\/fimmu.2025.1540676"},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Zhao, H., Yang, Y., Lyu, J., Ren, X., and Cheng, W. (2021). Development and Application of a Method to Detect 27 Respiratory Pathogens Using Multiplex RT-PCR Combined with MassARRAY Technology. BMC Infect. Dis., 21.","DOI":"10.1186\/s12879-021-06404-0"},{"key":"ref_81","unstructured":"(2025, August 28). EZERTM ADV Antigen Rapid Test. Available online: https:\/\/genesis-ivd.com\/index\/respiratory-pathogen-rapid-test\/13.html."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/0166-0934(82)90051-9","article-title":"Enzyme-Linked Immunosorbent Assay for Acute Adenovirus Infection","volume":"4","author":"Roggendorf","year":"1982","journal-title":"J. Virol. Methods"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.omto.2022.03.003","article-title":"Intraperitoneal Oncolytic Virotherapy for Patients with Malignant Ascites: Characterization of Clinical Efficacy and Antitumor Immune Response","volume":"25","author":"Zhang","year":"2022","journal-title":"Mol. Ther. Oncolytics"}],"container-title":["Biosensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6374\/15\/9\/605\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:45:30Z","timestamp":1760035530000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6374\/15\/9\/605"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,14]]},"references-count":83,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2025,9]]}},"alternative-id":["bios15090605"],"URL":"https:\/\/doi.org\/10.3390\/bios15090605","relation":{},"ISSN":["2079-6374"],"issn-type":[{"value":"2079-6374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,14]]}}}