{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T12:30:57Z","timestamp":1784809857359,"version":"3.55.0"},"reference-count":35,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T00:00:00Z","timestamp":1758326400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Grant-in-Aid for Scientific Research (B) KAKENHI","award":["24K00912"],"award-info":[{"award-number":["24K00912"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>Traditionally, fuel cells operate by using small fuel molecules such as hydrogen and methanol to produce energy, water, and carbon dioxide. Enzyme biofuel cells use enzymes rather than precious metals as electrode catalysts. In recent years, enzyme-immobilized electrodes have been developed by combining enzyme biofuel cells with microfluidic technology to improve the efficiency and performance of fuel cells. In this review, we will provide an overview and describe the current status of recent enzyme biofuel cells, microfluidic technology, and their applications to microfluidic fuel cells.<\/jats:p>","DOI":"10.3390\/bios15090627","type":"journal-article","created":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T12:08:03Z","timestamp":1758542883000},"page":"627","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Recent Advances in Microfluidic Biofuel Cells"],"prefix":"10.3390","volume":"15","author":[{"given":"Takahiro","family":"Kawaguchi","sequence":"first","affiliation":[{"name":"Department of Chemistry, Faculty of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku-ku, Tokyo 162-8601, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shota","family":"Ito","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku-ku, Tokyo 162-8601, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisuke","family":"Nakane","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku-ku, Tokyo 162-8601, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4410-4912","authenticated-orcid":false,"given":"Takashiro","family":"Akitsu","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku-ku, Tokyo 162-8601, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1021\/acsmeasuresciau.3c00044","article-title":"Abiotic, Hybrid, and Biological Electrocatalytic Materials Applied in Microfluidic Fuel Cells: A Com-prehensive","volume":"4","author":"Gurrola","year":"2024","journal-title":"ACS Meas. 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