{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:34:19Z","timestamp":1787027659389,"version":"build-2736575974"},"reference-count":41,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T00:00:00Z","timestamp":1760659200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22322409"],"award-info":[{"award-number":["22322409"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21974109"],"award-info":[{"award-number":["21974109"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["SWU-KF25011"],"award-info":[{"award-number":["SWU-KF25011"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>Fluorescence anisotropy (FA) has been widely used for analyzing biomolecules due to its high throughput, homogeneous detection, and strong resistance to photobleaching. However, the traditional FA method suffers from low sensitivity when the target molecules are small and rotate rapidly, often producing insignificant changes in the FA value. In this study, by combining double signal amplification through the trans-cleavage of CRISPR-Cas12a and DNA tetrahedron assemblies with a large molecular size, a new, fast, simple and highly sensitive FA method was constructed to achieve the quantitative detection of hepatitis B virus DNA (HBV-DNA). The experimental results showed that the linear range of this method was 0.5\u20139 nmol\/L, and the detection limit (LOD = 3\u03c3\/k) was 48 pmol\/L. In addition, the method demonstrated excellent selectivity and anti-interference, and it was successfully applied to detect HBV-DNA in human serum, indicating that this method has the potential for clinical diagnosis.<\/jats:p>","DOI":"10.3390\/bios15100700","type":"journal-article","created":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T11:39:34Z","timestamp":1760701174000},"page":"700","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["CRISPR-Cas12a and DNA Tetrahedron Assemblies Amplified Fluorescence Anisotropy for the Sensitive Detection of Hepatitis B Virus DNA"],"prefix":"10.3390","volume":"15","author":[{"given":"Yu","family":"Qin","sequence":"first","affiliation":[{"name":"College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China"},{"name":"Key Laboratory of Biomedical Analytics of Chongqing Science and Technology Bureau, Southwest University, Chongqing 400715, China"},{"name":"Academy for Advanced Interdisciplinary Studies, Southwest University, Chongqing 400715, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiali","family":"Xie","sequence":"additional","affiliation":[{"name":"College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China"},{"name":"Key Laboratory of Biomedical Analytics of Chongqing Science and Technology Bureau, Southwest University, Chongqing 400715, China"},{"name":"Academy for Advanced Interdisciplinary Studies, Southwest University, Chongqing 400715, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1236-5501","authenticated-orcid":false,"given":"Shujun","family":"Zhen","sequence":"additional","affiliation":[{"name":"College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China"},{"name":"Key Laboratory of Biomedical Analytics of Chongqing Science and Technology Bureau, Southwest University, Chongqing 400715, China"},{"name":"Academy for Advanced Interdisciplinary Studies, Southwest University, Chongqing 400715, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"10735","DOI":"10.1021\/jacs.1c04942","article-title":"Encoding fluorescence anisotropic barcodes with DNA frameworks","volume":"143","author":"Huang","year":"2021","journal-title":"J. Am. Chem. Soc."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Lakowicz, J.R., and Masters, B.R. (2008). Principles of fluorescence spectroscopy, Third Edition. J. Biomed. Opt., 13.","DOI":"10.1117\/1.2904580"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1575","DOI":"10.1016\/j.cclet.2019.06.005","article-title":"Recent progress in nanomaterial-enhanced fluorescence polarization\/anisotropy sensors","volume":"30","author":"Chen","year":"2019","journal-title":"Chin. Chem. Lett."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"121721","DOI":"10.1016\/j.talanta.2020.121721","article-title":"A near-infrared-II fluorescence anisotropy strategy for separation-free detection of adenosine triphosphate in complex media","volume":"223","author":"Fan","year":"2021","journal-title":"Talanta"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"4903","DOI":"10.1021\/acs.analchem.5b00479","article-title":"nanoparticles-free fluorescence anisotropy amplification assay for detection of RNA nucleotide-cleaving DNAzyme activity","volume":"87","author":"Zhang","year":"2015","journal-title":"Anal. Chem."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"113564","DOI":"10.1016\/j.bios.2021.113564","article-title":"Framework nucleic acid-wrapped protein-inorganic hybrid nanoflowers with three-stage amplified fluorescence polarization for terminal deoxynucleotidyl transferase activity biosensing","volume":"193","author":"Zhang","year":"2021","journal-title":"Biosens. Bioelectron."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"13708","DOI":"10.1021\/acs.analchem.8b04107","article-title":"Chimeric aptamers-based and MoS2 nanosheet-enhanced label-free fluorescence polarization strategy for adenosine triphosphate detection","volume":"90","author":"Fan","year":"2018","journal-title":"Anal. Chem."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"11518","DOI":"10.1021\/ac402781g","article-title":"Target-triggered cyclic assembly of DNA\u2013protein hybrid nanowires for dual-amplified fluorescence anisotropy assay of small molecules","volume":"85","author":"Yang","year":"2013","journal-title":"Anal. Chem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"122349","DOI":"10.1016\/j.talanta.2021.122349","article-title":"Label free structure-switching fluorescence polarization detection of chloramphenicol with truncated aptamer","volume":"230","author":"Ma","year":"2021","journal-title":"Talanta"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"17037","DOI":"10.1039\/D0RA01950J","article-title":"Sensitive and enzyme-free fluorescence polarization detection for miRNA-21 based on decahedral sliver nanoparticles and strand displacement reaction","volume":"10","author":"Zhu","year":"2020","journal-title":"RSC Adv."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2774","DOI":"10.1021\/acs.analchem.2c03912","article-title":"In-vial detection of protein denaturation using intrinsic fluorescence anisotropy","volume":"95","author":"Chullipalliyalil","year":"2023","journal-title":"Anal. Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1093\/nar\/gkj454","article-title":"Base-stacking and base-pairing contributions into thermal stability of the DNA double helix","volume":"34","author":"Yakovchuk","year":"2006","journal-title":"Nucleic Acids Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1126\/science.287.5454.820","article-title":"Adaptive recognition by nucleic acid aptamers","volume":"287","author":"Hermann","year":"2000","journal-title":"Science"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1146\/annurev.bi.53.070184.001453","article-title":"Protein-DNA recognition","volume":"53","author":"Pabo","year":"1984","journal-title":"Ann. Rev. Biochem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1016\/j.jinorgbio.2016.03.003","article-title":"Thermodynamics of the formation of Ag(I)-mediated azole base pairs in DNA duplexes","volume":"160","author":"Schweizer","year":"2016","journal-title":"J. Inorg. Biochem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"11723","DOI":"10.1021\/acs.analchem.3c01760","article-title":"Target-induced AIE Effect coupled with CRISPR\/Cas12a system dual-signal biosensing for the ultrasensitive detection of gliotoxin","volume":"95","author":"Ma","year":"2023","journal-title":"Anal. Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3672","DOI":"10.1038\/s41467-019-11648-1","article-title":"A CRISPR-Cas12a-derived biosensing platform for the highly sensitive detection of diverse small molecules","volume":"10","author":"Liang","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Lau, C.H., Liang, Q.L., Chen, X., Li, J., Shi, Y., Huang, Y., Xia, Q., Zhu, H., and Chen, G. (2025). CRISPR\/Dx-Based Colorimetric and Electrochemical Detection Systems for POCT Applications. Biosens. Bioelectron., 288.","DOI":"10.1016\/j.bios.2025.117778"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"934","DOI":"10.1038\/s41467-024-45163-9","article-title":"Fluorogenic CRISPR for Genomic DNA Imaging","volume":"15","author":"Zhang","year":"2024","journal-title":"Nat. Commun."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1007\/s00604-024-06548-1","article-title":"Primer Exchange Reaction Assisted CRISPR\/Cas9 Cleavage for Detection of Dual microRNAs with Electrochemistry Method","volume":"191","author":"Zhou","year":"2024","journal-title":"Microchim. Acta"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Wei, L., Luo, S., Zhou, W., Ren, B., Li, M., Liang, L., Li, X., and Wei, G. (2025). Rapid Detection of Pseudomonas Aeruginosa by Glycerol One-Pot RAA\/CRISPR-Cas12a Method. Front. Chem., 13.","DOI":"10.3389\/fchem.2025.1654270"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"137007","DOI":"10.1016\/j.snb.2024.137007","article-title":"Signal Amplified Fluorometric Detection of Anti-CRISPR Proteins Based on Rolling Circle Amplification","volume":"425","author":"Cao","year":"2025","journal-title":"Sens. Actuators B"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1007\/s00604-025-07198-7","article-title":"Concanavalin-A-Assisted Extraction-Free One-Pot RPA\u2013CRISPR\/Cas12a Assay for Rapid Detection of HPV16","volume":"192","author":"Nie","year":"2025","journal-title":"Microchim. Acta"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1021\/acssensors.5c00330","article-title":"Advancing CRISPR\/Cas Biosensing with Integrated Devices","volume":"10","author":"Liu","year":"2025","journal-title":"ACS Sens."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Li, Z., Su, X., Lin, Y., Zhang, Y., Zhang, A., Wu, X., Jiyu, X., Li, Q., and Wei, Z. (2025). Expanding the Cell Quantity of CRISPR\/Cas9 Gene Editing by Continuous Microfluidic Electroporation Chip. Bioelectrochemistry, 161.","DOI":"10.1016\/j.bioelechem.2024.108840"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"166699","DOI":"10.1016\/j.cej.2025.166699","article-title":"A Universal Cas12a Activity Regulation Strategy via Photocleavable crRNA 3\u2032-Terminal Additives Enables One-Pot CRISPR Detection of Arboviruses","volume":"521","author":"Zhuang","year":"2025","journal-title":"Chem. Eng. J."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"135723","DOI":"10.1016\/j.snb.2024.135723","article-title":"An Electrochemical, Fluorescent, and Colorimetric Triple-Mode Homogeneous Biosensor for the Simultaneous Detection of Influenza A, Influenza B, and SARS-CoV-2","volume":"411","author":"Dong","year":"2024","journal-title":"Sens. Actuators B"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"118008","DOI":"10.1016\/j.bios.2025.118008","article-title":"CRISPR-Integrated Nanoconfined Interparticle Catalytic Hairpin Assembly for Enhanced Dual-Mode SARS-CoV-2 Detection in Wastewater","volume":"290","author":"Zheng","year":"2025","journal-title":"Biosens. Bioelectron."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"11741","DOI":"10.1021\/acs.analchem.3c01876","article-title":"A rationally designed CRISPR\/Cas12a assay using a multimodal reporter for various readouts","volume":"95","author":"De","year":"2023","journal-title":"Anal. Chem."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Yi, Z., De, J., Mukama, O., Li, Z., Odiwuor, N., Jing, H., Nie, C., Hu, M., Lin, Z., and Wei, H. (2021). Rational programming of Cas12a for early-stage detection of COVID-19 by lateral flow assay and portable real-time fluorescence readout facilities. Biosensors, 12.","DOI":"10.3390\/bios12010011"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"114033","DOI":"10.1016\/j.bios.2022.114033","article-title":"Mechanistic insights of CRISPR\/Cas nucleases for programmable targeting and early-stage diagnosis: A review","volume":"203","author":"Habimana","year":"2022","journal-title":"Biosens. Bioelectron."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1007\/s40820-022-00888-4","article-title":"CRISPR-Cas12a-empowered electrochemical biosensor for rapid and ultrasensitive detection of SARS-CoV-2 delta variant","volume":"14","author":"Wu","year":"2022","journal-title":"Nano Micro Lett."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2986","DOI":"10.1038\/s41596-019-0210-2","article-title":"Sherlock: Nucleic acid detection with CRISPR nucleases","volume":"14","author":"Kellner","year":"2019","journal-title":"Nat. Protoc."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"115523","DOI":"10.1016\/j.bios.2023.115523","article-title":"CRISPR\/Cas12a-assisted isothermal amplification for rapid and specific diagnosis of respiratory virus on an microfluidic platform","volume":"237","author":"Shen","year":"2023","journal-title":"Biosens. Bioelectron."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"125011","DOI":"10.1016\/j.talanta.2023.125011","article-title":"A CG@MXene nanocomposite-driven e-CRISPR biosensor for the rapid and sensitive detection of salmonella typhimurium in food","volume":"266","author":"Duan","year":"2024","journal-title":"Talanta"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"7237","DOI":"10.1021\/acs.analchem.3c00156","article-title":"CRISPR-Cas12a coupled with DNA nanosheet-amplified fluorescence anisotropy for sensitive detection of biomolecules","volume":"95","author":"Xie","year":"2023","journal-title":"Anal. Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"109137","DOI":"10.1016\/j.cclet.2023.109137","article-title":"Octopus-like DNA nanostructure coupled with graphene oxide enhanced fluorescence anisotropy for hepatitis B virus DNA detection","volume":"35","author":"Xie","year":"2024","journal-title":"Chin. Chem. Lett."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1820","DOI":"10.1039\/C7RA11945C","article-title":"A label-free electrochemical platform for the highly sensitive detection of hepatitis B virus DNA using graphene quantum dots","volume":"8","author":"Xiang","year":"2018","journal-title":"RSC Adv."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1021\/cg801006g","article-title":"Fast synthesis of Cu2O hollow microspheres and their application in DNA biosensor of hepatitis B virus","volume":"9","author":"Zhu","year":"2009","journal-title":"Cryst. Growth Des."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.snb.2014.08.079","article-title":"Exonuclease III-assisted recycling amplification detection of hepatitis B virus DNA by DNA-scaffolded silver nanoclusters probe","volume":"205","author":"Liu","year":"2014","journal-title":"Sens. Actuators B"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1934","DOI":"10.1016\/j.bios.2010.01.007","article-title":"Ds-DNA nanosensor for the detection of hepatitis B virus DNA and the single-base mutants","volume":"25","author":"Wang","year":"2010","journal-title":"Biosens. Bioelectron."}],"container-title":["Biosensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6374\/15\/10\/700\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T12:22:00Z","timestamp":1760703720000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6374\/15\/10\/700"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,17]]},"references-count":41,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2025,10]]}},"alternative-id":["bios15100700"],"URL":"https:\/\/doi.org\/10.3390\/bios15100700","relation":{},"ISSN":["2079-6374"],"issn-type":[{"value":"2079-6374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,17]]}}}