{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T14:30:40Z","timestamp":1787063440898,"version":"build-2736575974"},"reference-count":27,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T00:00:00Z","timestamp":1761091200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82174092"],"award-info":[{"award-number":["82174092"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82473892"],"award-info":[{"award-number":["82473892"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Natural Science Foundation of Shanghai","award":["21ZR1483000"],"award-info":[{"award-number":["21ZR1483000"]}]},{"name":"National Natural Science Foundation of Shanghai","award":["22ZR1476900"],"award-info":[{"award-number":["22ZR1476900"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>Surface plasmon resonance (SPR) biosensors have been applied in various fields with the advantages of being label-free, having high specificity, having high sensitivity, and providing real-time monitoring. With the gradual improvement of SPR technology, SPR biosensors have been used for the detection of macromolecules such as proteins, peptides, and nucleic acids. Antibodies are generally used as the recognition component of SPR biosensors due to the high specificity of antibody\u2013antigen binding. Recently, aptamers have become new choices instead of antibodies for their characteristic of high specificity with target molecules, high stability of chemical synthesis, convenience in storage, and ease of labeling. In this study, an aptamer-based SPR biosensor for chloramphenicol (CAP) detection was established through optimizing the conditions of CAP aptamer immobilization and analysis procedure, including biosensor type, signal enhancement, running buffer, sample diluent, and dissociation time. The results suggested that the optimal immobilization strategy of aptamers on the SPR biosensor was indirect immobilization based on the CM5 chip. The aptamer-based SPR biosensor had good specificity for CAP and could be used to detect CAP in real samples such as milk. Therefore, SPR biosensors have great application prospects in the food safety field, and aptamers deserve further study to improve the performance of the biosensor.<\/jats:p>","DOI":"10.3390\/bios15110706","type":"journal-article","created":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T08:41:08Z","timestamp":1761122468000},"page":"706","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Development of an Aptamer-Based Surface Plasmon Resonance Biosensor for Detecting Chloramphenicol in Milk"],"prefix":"10.3390","volume":"15","author":[{"given":"Minyu","family":"Qi","sequence":"first","affiliation":[{"name":"Department of Biochemical Pharmacy, College of Pharmacy, Naval Medical University, Shanghai 200433, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-2668-8817","authenticated-orcid":false,"given":"Ningqi","family":"Xia","sequence":"additional","affiliation":[{"name":"Department of Biochemical Pharmacy, College of Pharmacy, Naval Medical University, Shanghai 200433, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiying","family":"Wang","sequence":"additional","affiliation":[{"name":"Suzhou Innovation Center of Shanghai University, Suzhou 215127, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4536-8960","authenticated-orcid":false,"given":"Xiaofei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biochemical Pharmacy, College of Pharmacy, Naval Medical University, Shanghai 200433, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Biochemical Pharmacy, College of Pharmacy, Naval Medical University, Shanghai 200433, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diya","family":"Lv","sequence":"additional","affiliation":[{"name":"Center for Instrumental Analysis, College of Pharmacy, Naval Medical University, Shanghai 200433, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5510-9773","authenticated-orcid":false,"given":"Yan","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Biochemical Pharmacy, College of Pharmacy, Naval Medical University, Shanghai 200433, China"},{"name":"Shanghai Key Laboratory for Pharmaceutical Metabolite Research, College of Pharmacy, Naval Medical University, Shanghai 200433, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"121344","DOI":"10.1016\/j.jhazmat.2019.121344","article-title":"High-efficiency biodegradation of chloramphenicol by enriched bacterial consortia: Kinetics study and bacterial community characterization","volume":"384","author":"Zhang","year":"2020","journal-title":"J. Hazard. Mater."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"5670","DOI":"10.1002\/fsn3.2530","article-title":"Determination of chloramphenicol in meat samples using liquid chromatography\u2013tandem mass spectrometry","volume":"9","author":"Mou","year":"2021","journal-title":"Food Sci. Nutr."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1929","DOI":"10.1007\/s10311-022-01416-x","article-title":"Occurrence, toxicity and adsorptive removal of the chloramphenicol antibiotic in water: A review","volume":"20","author":"Nguyen","year":"2022","journal-title":"Environ. Chem. Lett."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/j.foodcont.2015.04.028","article-title":"Transfer of chloramphenicol from milk to commercial dairy products\u2014Experimental proof","volume":"57","author":"Sniegocki","year":"2015","journal-title":"Food Control."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"127857","DOI":"10.1016\/j.foodchem.2020.127857","article-title":"Rapid quantitative detection of chloramphenicol in milk by microfluidic immunoassay","volume":"339","author":"Zhao","year":"2021","journal-title":"Food Chem."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2303","DOI":"10.1007\/s00216-014-7647-5","article-title":"Surface plasmon resonance applications in clinical analysis","volume":"406","author":"Mariani","year":"2014","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.bios.2018.12.023","article-title":"Nanomaterials based surface plasmon resonance signal enhancement for detection of environmental pollutions","volume":"127","author":"Mahmoudpour","year":"2019","journal-title":"Biosens. Bioelectron."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Zhou, J., Qi, Q., Wang, C., Qian, Y., Liu, G., Wang, Y., and Fu, L. (2019). Surface plasmon resonance (SPR) biosensors for food allergen detection in food matrices. Biosens. Bioelectron., 142.","DOI":"10.1016\/j.bios.2019.111449"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"124366","DOI":"10.1016\/j.talanta.2023.124366","article-title":"A portable smartphone-based imaging surface plasmon resonance biosensor for allergen detection in plant-based milks","volume":"257","author":"Xiao","year":"2023","journal-title":"Talanta"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1016\/j.talanta.2018.11.110","article-title":"Development of a surface plasmon resonance immunosensor and ELISA for 3-nitrotyrosine in human urine","volume":"195","author":"He","year":"2019","journal-title":"Talanta"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1016\/j.scitotenv.2017.09.157","article-title":"A non-competitive surface plasmon resonance immunosensor for rapid detection of triazophos residue in environmental and agricultural samples","volume":"613\u2013614","author":"Guo","year":"2018","journal-title":"Sci. Total. Environ."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"ACI.S31614","DOI":"10.4137\/ACI.S31614","article-title":"Quality Issues of Research Antibodies","volume":"11","author":"Weller","year":"2016","journal-title":"Anal. Chem. Insights"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1038\/521274a","article-title":"Reproducibility crisis: Blame it on the antibodies","volume":"521","author":"Baker","year":"2015","journal-title":"Nature"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"9500","DOI":"10.1021\/acsami.0c05750","article-title":"Recent Progress in Aptamer Discoveries and Modifications for Therapeutic Applications","volume":"13","author":"Ni","year":"2021","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.biotechadv.2018.11.001","article-title":"Three decades of nucleic acid aptamer technologies: Lessons learned, progress and opportunities on aptamer development","volume":"37","author":"Wang","year":"2019","journal-title":"Biotechnol. Adv."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"11959","DOI":"10.3390\/molecules200711959","article-title":"A Highlight of Recent Advances in Aptamer Technology and Its Application","volume":"20","author":"Sun","year":"2015","journal-title":"Molecules"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Bauer, M., Strom, M., Hammond, D.S., and Shigdar, S. (2019). Anything You Can Do, I Can Do Better: Can Aptamers Replace Antibodies in Clinical Diagnostic Applications?. Molecules, 24.","DOI":"10.3390\/molecules24234377"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.addr.2018.04.007","article-title":"Aptamer chemistry","volume":"134","author":"Hollenstein","year":"2018","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"26838","DOI":"10.1021\/acsomega.4c02466","article-title":"Computational Frontiers in Aptamer-Based Nanomedicine for Precision Therapeutics: A Comprehensive Review","volume":"9","author":"Kumar","year":"2024","journal-title":"ACS Omega"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.toxicon.2018.03.006","article-title":"A direct determination of AFBs in vinegar by aptamer-based surface plasmon resonance biosensor","volume":"146","author":"Wu","year":"2018","journal-title":"Toxicon"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"125232","DOI":"10.1016\/j.talanta.2023.125232","article-title":"Simultaneous detection method for two cardiac disease protein biomarkers on a single chip modified with mixed aptamers using surface plasmon resonance","volume":"267","author":"Lee","year":"2024","journal-title":"Talanta"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/j.snb.2018.09.077","article-title":"High-sensitivity glycated hemoglobin (HbA1c) aptasensor in rapid-prototyping surface plasmon resonance","volume":"279","author":"Zhang","year":"2019","journal-title":"Sens. Actuators B Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1002\/elan.201900736","article-title":"Finding the Optimal Surface Density of Aptamer Monolayers by SPR Imaging Detection-Based Aptamer Microarrays","volume":"32","author":"Simon","year":"2020","journal-title":"Electroanalysis"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1007\/s00216-018-1533-5","article-title":"Electrochemical surface plasmon resonance (EC-SPR) aptasensor for ampicillin detection","volume":"411","author":"Blidar","year":"2019","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"5315","DOI":"10.1007\/s00216-025-06052-2","article-title":"Surface plasmon resonance sensor for direct oxytetracycline detection with a newly selected aptamer","volume":"417","author":"Kang","year":"2025","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/j.jbiotec.2011.06.043","article-title":"In vitro selection and characterization of DNA aptamers recognizing chloramphenicol","volume":"155","author":"Mehta","year":"2011","journal-title":"J. Biotechnol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"122349","DOI":"10.1016\/j.talanta.2021.122349","article-title":"Label free structure-switching fluorescence polarization detection of chloramphenicol with truncated aptamer","volume":"230","author":"Ma","year":"2021","journal-title":"Talanta"}],"container-title":["Biosensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6374\/15\/11\/706\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T08:51:51Z","timestamp":1761123111000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6374\/15\/11\/706"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,22]]},"references-count":27,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2025,11]]}},"alternative-id":["bios15110706"],"URL":"https:\/\/doi.org\/10.3390\/bios15110706","relation":{},"ISSN":["2079-6374"],"issn-type":[{"value":"2079-6374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,22]]}}}