{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T16:04:14Z","timestamp":1765209854553,"version":"3.46.0"},"reference-count":35,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,12,7]],"date-time":"2025-12-07T00:00:00Z","timestamp":1765065600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["21wm0225010h0102"],"award-info":[{"award-number":["21wm0225010h0102"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>The four serotypes of dengue virus (DENV), types 1 to 4 (DENV-1 to DENV-4), exhibit approximately 60% identity in the encoded amino acid residues of viral proteins. Reverse transcription of RNA extracted from patient serum specimens followed by PCR amplification with serotype-specific probes is the current standard technique for DENV serotyping. However, this method is time- and cost-consuming, and rapid detection systems with low cost are desirable. Previously, we developed a prototype serotype-specific immunochromatography system. That system was composed of four strips with four corresponding distinct sample buffers, each specifically detecting a single DENV serotype. In the present study, we improved this system by combining pairs of strips into one lateral-flow cassette each, providing DENV-1 and DENV-2 detection in one device and DENV-3 and DENV-4 detection in a second device; this strategy successfully reduced the required sample volume. Furthermore, we were able to adjust the composition of the sample buffers such that a single sample buffer sufficed for all four DENV serotype detection reactions, allowing much easier handling of the devices. Evaluation of this new device against laboratory and clinical DENV isolates and clinical specimens from DENV-infected individuals showed sensitivity that was comparable to that of our previous version, yielding serotype specificity of 100%. These new devices are expected to be of use in the clinical setting, accelerating both prospective and retrospective epidemiological studies.<\/jats:p>","DOI":"10.3390\/bios15120802","type":"journal-article","created":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T15:30:07Z","timestamp":1765207807000},"page":"802","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["An Improved Dengue Virus Serotype-Specific Non-Structural Protein 1 Capture Immunochromatography Method with Reduced Sample Volume"],"prefix":"10.3390","volume":"15","author":[{"given":"Warisara","family":"Sretapunya","sequence":"first","affiliation":[{"name":"Nakhon Nayok Hospital, Nakhon Nayok 26000, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thitiya","family":"Buranachat","sequence":"additional","affiliation":[{"name":"Nakhon Nayok Hospital, Nakhon Nayok 26000, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Montita","family":"Prasomthong","sequence":"additional","affiliation":[{"name":"Nakhon Nayok Hospital, Nakhon Nayok 26000, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rittichai","family":"Tantikorn","sequence":"additional","affiliation":[{"name":"Nakhon Nayok Hospital, Nakhon Nayok 26000, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Areerat","family":"Sa-ngarsang","sequence":"additional","affiliation":[{"name":"National Institute of Health, Department of Medical Science, Ministry of Public Health, Nonthaburi 11000, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sirirat","family":"Naemkhunthot","sequence":"additional","affiliation":[{"name":"National Institute of Health, Department of Medical Science, Ministry of Public Health, Nonthaburi 11000, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laddawan","family":"Meephaendee","sequence":"additional","affiliation":[{"name":"National Institute of Health, Department of Medical Science, Ministry of Public Health, Nonthaburi 11000, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pattara","family":"Wongjaroen","sequence":"additional","affiliation":[{"name":"National Institute of Health, Department of Medical Science, Ministry of Public Health, Nonthaburi 11000, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chika","family":"Tanaka","sequence":"additional","affiliation":[{"name":"VisGene Ltd., Osaka 562-0033, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoriko","family":"Shimadzu","sequence":"additional","affiliation":[{"name":"VisGene Ltd., Osaka 562-0033, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsuya","family":"Ogata","sequence":"additional","affiliation":[{"name":"VisGene Ltd., Osaka 562-0033, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kunihiro","family":"Kaihatsu","sequence":"additional","affiliation":[{"name":"VisGene Ltd., Osaka 562-0033, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryo","family":"Morita","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Osaka City General Hospital, Osaka 534-0021, Japan"},{"name":"Department of Infectious Diseases, Osaka City Juso Hospital, Osaka 532-0034, Japan"},{"name":"Department of Viral Infections, Research Institute for Microbial Diseases, The University of Osaka, Osaka 565-0871, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-6421-7478","authenticated-orcid":false,"given":"Michinori","family":"Shirano","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Osaka City General Hospital, Osaka 534-0021, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6739-0144","authenticated-orcid":false,"given":"Juthamas","family":"Phadungsombat","sequence":"additional","affiliation":[{"name":"Department of Viral Infections, Research Institute for Microbial Diseases, The University of Osaka, Osaka 565-0871, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tadahiro","family":"Sasaki","sequence":"additional","affiliation":[{"name":"Department of Viral Infections, Research Institute for Microbial Diseases, The University of Osaka, Osaka 565-0871, 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