{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T18:05:31Z","timestamp":1784829931393,"version":"3.55.0"},"reference-count":59,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T00:00:00Z","timestamp":1765929600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"H2020 Future Emerging Technologies of the European Commission","award":["862539"],"award-info":[{"award-number":["862539"]}]},{"DOI":"10.13039\/100016172","name":"Luxembourg Institute of Science and Technology","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100016172","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>Sensors to monitor the immune status of an individual play a crucial role in understanding the acquired immunity or signs of a latent infection. Such sensors can be an effective tool to manage infection and to design treatment options in vulnerable populations. We demonstrate here highly sensitive detection of acquired immunity to Cytomegalovirus CMV by detection of anti-CMV antibodies using plasmon-enhanced fluorescence (PEF). The PEF sensors leverage plasmonic enhancement from a high density of intense electromagnetic hotspots in self-assembly-derived gold nanopillar arrays. Comparing PEF assays with assays on a planar surface plasmon resonance sensor shows the PEF sensors to be sensitive to a small fraction of the antibodies on the surface. The detection scheme deploys peptide monolayers with specific affinity to anti-CMV antibodies to capture them onto the sensor surfaces. The results of the assay on the PEF sensor reveal high promise for sensors with miniaturized sensing footprints, ease of spatial multiplexing, high sensitivity, and quick response times. The developments are readily applicable to a range of other diagnostic contexts where peptide\u2013protein interactions and self-assembly-derived PEF sensors can be leveraged.<\/jats:p>","DOI":"10.3390\/bios15120817","type":"journal-article","created":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T09:43:46Z","timestamp":1765964626000},"page":"817","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Peptide-Based Plasmon-Enhanced Spectroscopic Immunoassay to Detect Immunity Against Cytomegalovirus"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6219-0204","authenticated-orcid":false,"given":"Aruna","family":"Chandra Singh","sequence":"first","affiliation":[{"name":"Luxembourg Institute of Science and Technology (LIST), 41, rue du Brill, L-4422 Belvaux, Luxembourg"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-3291-1829","authenticated-orcid":false,"given":"Clara","family":"Sidhoum","sequence":"additional","affiliation":[{"name":"Luxembourg Institute of Science and Technology (LIST), 41, rue du Brill, L-4422 Belvaux, Luxembourg"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1548-1498","authenticated-orcid":false,"given":"Hugo","family":"Payen","sequence":"additional","affiliation":[{"name":"Luxembourg Institute of Science and Technology (LIST), 41, rue du Brill, L-4422 Belvaux, Luxembourg"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5540-4633","authenticated-orcid":false,"given":"Divya","family":"Balakrishnan","sequence":"additional","affiliation":[{"name":"Luxembourg Institute of Science and Technology (LIST), 41, rue du Brill, L-4422 Belvaux, Luxembourg"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3542-3874","authenticated-orcid":false,"given":"Saulius","family":"Juodkazis","sequence":"additional","affiliation":[{"name":"Optical Sciences Centre, Swinburne University of Technology, Hawthorn, VIC 3122, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"\u00d8sterbye","sequence":"additional","affiliation":[{"name":"Department of Immunology and Microbiology, University of Copenhagen, 22200 Copenhagen N, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0410-1696","authenticated-orcid":false,"given":"Sivashankar","family":"Krishnamoorthy","sequence":"additional","affiliation":[{"name":"Luxembourg Institute of Science and Technology (LIST), 41, rue du Brill, L-4422 Belvaux, Luxembourg"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1471-2393-13-96","article-title":"Cytomegalovirus in Pregnancy: To Screen or Not to Screen","volume":"13","author":"Walker","year":"2013","journal-title":"BMC Pregnancy Childbirth"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"463","DOI":"10.5468\/ogs.23117","article-title":"Cytomegalovirus Infection during Pregnancy","volume":"66","author":"Choodinatha","year":"2023","journal-title":"Obstet. 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