{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T22:12:47Z","timestamp":1788991967089,"version":"build-2803163510"},"reference-count":26,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,12,28]],"date-time":"2025-12-28T00:00:00Z","timestamp":1766880000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012190","name":"Ministry of Science and Higher Education of the Russian Federation to the EIMB Center for Precision Genetic Technologies for Medicine","doi-asserted-by":"publisher","award":["075-15-2025-519"],"award-info":[{"award-number":["075-15-2025-519"]}],"id":[{"id":"10.13039\/501100012190","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>The detailed characterization of antigen-specific serum antibodies is hindered by the lack of efficient, gentle isolation methods. In this context, standard column affinity chromatography, although a powerful purification tool, presents practical challenges, including high antigen consumption and elution conditions that risk inducing antibody polyreactivity, while conventional acidic elution often compromises antibody integrity. This study introduces a novel microscale method for isolating specific immunoglobulins using anionic detergents as mild eluents. We employed antigen-functionalized hydrogel microarrays and magnetic beads as micro-immunosorbents. Among the tested detergents, sodium lauroyl glutamate (SLG) was optimal, achieving up to 78.3% recovery of functional antibodies. The optimized protocol, including recovery via G25-Sephadex gel filtration, effectively isolated specific antibodies from complex serum, retaining 58.5\u201385.3% of their functional bioactivity. Multiplex immunoassays confirmed the high specificity of the isolated antibodies and the lack of detergent-induced polyreactivity. The method was successfully adapted to isolate both specific antibodies (virus, dietary, and autoimmune) and total IgG, demonstrating versatility across platforms. This work establishes a robust, efficient, and gentle workflow for obtaining high-purity, bioactive antibodies, enabling their subsequent in-depth analysis for research applications.<\/jats:p>","DOI":"10.3390\/bios16010022","type":"journal-article","created":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T00:50:42Z","timestamp":1766969442000},"page":"22","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Anionic Detergents as Eluents for Microscale Isolation of Antigen-Specific Serum Immunoglobulins"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-6446-5092","authenticated-orcid":false,"given":"Dmitry","family":"Trukhin","sequence":"first","affiliation":[{"name":"Center for Precision Genetic Technologies for Medicine, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9454-5938","authenticated-orcid":false,"given":"Marina","family":"Filippova","sequence":"additional","affiliation":[{"name":"Center for Precision Genetic Technologies for Medicine, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-7695-6182","authenticated-orcid":false,"given":"Alla","family":"Tskaeva","sequence":"additional","affiliation":[{"name":"Endocrinology Research Centre, Ministry of Health of Russia, 117292 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8520-8702","authenticated-orcid":false,"given":"Ekaterina","family":"Troshina","sequence":"additional","affiliation":[{"name":"Endocrinology Research Centre, Ministry of Health of Russia, 117292 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3183-318X","authenticated-orcid":false,"given":"Dmitry","family":"Gryadunov","sequence":"additional","affiliation":[{"name":"Center for Precision Genetic Technologies for Medicine, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3091-8245","authenticated-orcid":false,"given":"Elena","family":"Savvateeva","sequence":"additional","affiliation":[{"name":"Center for Precision Genetic Technologies for Medicine, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1111\/imr.13407","article-title":"The importance of IgG glycosylation-What did we learn after analyzing over 100,000 individuals","volume":"328","author":"Lauc","year":"2024","journal-title":"Immunol. 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