{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T02:18:48Z","timestamp":1790216328736,"version":"4.1.0"},"reference-count":40,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T00:00:00Z","timestamp":1768521600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"JSPS KAKENHI","doi-asserted-by":"publisher","award":["JP23K13566"],"award-info":[{"award-number":["JP23K13566"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>Microparticle detection technology uses materials that can specifically recognize complex biostructures, such as antibodies and aptamers, as trapping agents. The development of antibody production technology and simplification of sensing signal output methods have facilitated commercialization of disposable biosensors, making rapid diagnosis possible. Although this contributed to the early resolution of pandemics, traditional biosensors face issues with sensitivity, durability, and rapid response times. We aimed to fabricate microspaces using metallic materials to further enhance durability of mold fabrication technologies, such as molecular imprinting. Low-damage metal deposition was performed on target protozoa and Norovirus-like particles (NoV-LPs) to produce thin metallic films that adhere to the material. The procedure for fitting the object into the bio structured space formed on the thin metal film took less than a minute, and sensitivity was 10 fg\/mL for NoV-LPs. Furthermore, because it was a metal film, no decrease in reactivity was observed even when the same substrate was stored at room temperature and reused repeatedly after fabrication. These findings underscore the potential of integrating stable metallic structures with bio-recognition elements to significantly enhance robustness and reliability of environmental monitoring. This contributes to public health strategies aimed at early detection and containment of infectious diseases.<\/jats:p>","DOI":"10.3390\/bios16010063","type":"journal-article","created":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T09:28:12Z","timestamp":1768555692000},"page":"63","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Long-Term Stable Biosensing Using Multiscale Biostructure-Preserving Metal Thin Films"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7892-8488","authenticated-orcid":false,"given":"Kenshin","family":"Takemura","sequence":"first","affiliation":[{"name":"Integrated Research Center for Wellbeing, National Institute of Advanced Industrial Science and Technology (AIST), Tosu 841-0052, Saga, Japan"},{"name":"Sensing Technology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tosu 841-0052, Saga, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1791-9190","authenticated-orcid":false,"given":"Taisei","family":"Motomura","sequence":"additional","affiliation":[{"name":"Sensing Technology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tosu 841-0052, Saga, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4567-0986","authenticated-orcid":false,"given":"Yuko","family":"Takagi","sequence":"additional","affiliation":[{"name":"Molecular Biosystems Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8566, Ibaraki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Summers, W.C. 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