{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T03:33:03Z","timestamp":1793071983421,"version":"4.5.0"},"reference-count":34,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T00:00:00Z","timestamp":1769731200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the National Key Research and Development Program of the Ministry of Science and Technology","award":["2022YFB3207200"],"award-info":[{"award-number":["2022YFB3207200"]}]},{"name":"Startup Foundation for Advanced Talents of Linyi University","award":["Z6122047"],"award-info":[{"award-number":["Z6122047"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>We report a novel dual-receptor lateral flow biosensor (LFB) for the rapid, sensitive, and visual detection of MCF-7 breast cancer cells as a model for circulating tumor cells (CTCs). The biosensor employs a MUC1-specific aptamer conjugated to colloidal gold nanoparticles as the detection probe and an anti-MUC1 antibody immobilized at the test line as the capture probe, forming a unique \u201captamer\u2013cell\u2013antibody\u201d sandwich complex upon target recognition. This design enables instrument-free, visual readout within minutes, achieving a detection limit of 675 cells. The assay also demonstrates robust performance in spiked human blood samples, highlighting its potential as a simple, cost-effective dual-mode point-of-care testing (POCT) platform. This platform supports both rapid visual screening and optional strip-reader-based quantification, making it suitable for early detection and monitoring of breast cancer CTCs.<\/jats:p>","DOI":"10.3390\/bios16020085","type":"journal-article","created":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T17:38:34Z","timestamp":1769794714000},"page":"85","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Sensitive Visual Detection of Breast Cancer Cells via a Dual-Receptor (Aptamer\/Antibody) Lateral Flow Biosensor"],"prefix":"10.3390","volume":"16","author":[{"given":"Yurui","family":"Zhou","sequence":"first","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahui","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Han","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meijing","family":"Ma","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junhong","family":"Li","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-8533-7319","authenticated-orcid":false,"given":"Haidong","family":"Li","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueji","family":"Zhang","sequence":"additional","affiliation":[{"name":"Marshall Laboratory of Biomedical Engineering, Research Center for Biosensor and Nanotheranostic, School of Biomedical Engineering, Shenzhen University, Shenzhen 518060, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1063-208X","authenticated-orcid":false,"given":"Guodong","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"670","DOI":"10.1016\/j.cell.2016.11.037","article-title":"Emerging biological principles of metastasis","volume":"168","author":"Lambert","year":"2017","journal-title":"Cell"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Singh, V.M. 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