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Rapid, sensitive, and selective detection and monitoring of the content of specific biomarkers in real time are key to point-of-care testing diagnostics. We report the detection of heat shock proteins by fluorescence immunoassay with the appropriate phage antibodies, with a minimum detection limit of 1 ng\/mL. The fluorescence immunoassay data were confirmed by dot immunoassay and by circular dichroism studies. The results of the study may help in the adaptation of the fluorescence immunoassay to cancer diagnostics.<\/jats:p>","DOI":"10.3390\/bios16040194","type":"journal-article","created":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T08:22:36Z","timestamp":1774858956000},"page":"194","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Fluorescence Immunosensor with Phage Antibodies for Heat Shock Protein 70 Detection"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0541-0020","authenticated-orcid":false,"given":"Olga I.","family":"Guliy","sequence":"first","affiliation":[{"name":"Institute of Biochemistry and Physiology of Plants and Microorganisms\u2014Subdivision of the Federal State Budgetary Research Institution Saratov Federal Scientific Centre of the Russian Academy of Sciences (IBPPM RAS), Saratov 410049, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergei A.","family":"Eremin","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Lomonosov Moscow State University, Moscow 119991, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5896-2523","authenticated-orcid":false,"given":"Liliya I.","family":"Mukhametova","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Lomonosov Moscow State University, Moscow 119991, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evgeniy S.","family":"Kozlov","sequence":"additional","affiliation":[{"name":"Department of Animal Diseases and Veterinary-Sanitary Expertise, Institute of Veterinary Medicine and Pharmacy, Vavilov Saratov State University of Genetics, Biotechnology and Engineering, Saratov 410012, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0627-6804","authenticated-orcid":false,"given":"Vyacheslav S.","family":"Grinev","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Physiology of Plants and Microorganisms\u2014Subdivision of the Federal State Budgetary Research Institution Saratov Federal Scientific Centre of the Russian Academy of Sciences (IBPPM RAS), Saratov 410049, Russia"},{"name":"Institute of Chemistry, Chernyshevsky Saratov National Research State University, 83 Ulitsa Astrakhanskaya, Saratov 410012, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergey A.","family":"Staroverov","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Physiology of Plants and Microorganisms\u2014Subdivision of the Federal State Budgetary Research Institution Saratov Federal Scientific Centre of the Russian Academy of Sciences (IBPPM RAS), Saratov 410049, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0665-1846","authenticated-orcid":false,"given":"Olga A.","family":"Karavaeva","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Physiology of Plants and Microorganisms\u2014Subdivision of the Federal State Budgetary Research Institution Saratov Federal Scientific Centre of the Russian Academy of Sciences (IBPPM RAS), Saratov 410049, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ksenia K.","family":"Fursova","sequence":"additional","affiliation":[{"name":"Branch of Shemyakin\u2013Ovchinnikov Institute of Bioorganic Chemistry, Pushchino 142290, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fedor A.","family":"Brovko","sequence":"additional","affiliation":[{"name":"Branch of Shemyakin\u2013Ovchinnikov Institute of Bioorganic Chemistry, Pushchino 142290, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2440-6761","authenticated-orcid":false,"given":"Lev A.","family":"Dykman","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Physiology of Plants and Microorganisms\u2014Subdivision of the Federal State Budgetary Research Institution Saratov Federal Scientific Centre of the Russian Academy of Sciences (IBPPM RAS), Saratov 410049, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingyun","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Chemical and Biological Engineering, Shandong University of Science and Technology, Qingdao 266590, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,28]]},"reference":[{"key":"ref_1","unstructured":"(2026, February 18). 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