{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T11:38:55Z","timestamp":1785411535896,"version":"3.56.0"},"reference-count":51,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T00:00:00Z","timestamp":1782086400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002858","name":"Postdoctoral Science Foundation of China","doi-asserted-by":"publisher","award":["2024M760620"],"award-info":[{"award-number":["2024M760620"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Hubei University of Science and Technology Doctoral Startup Fund Project","award":["BK202317"],"award-info":[{"award-number":["BK202317"]}]},{"award":["BK202317"],"award-info":[{"award-number":["BK202317"]}],"id":[{"id":"https:\/\/ror.org\/018wg9441","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010846","name":"Project of the Hubei Provincial Health Commission","doi-asserted-by":"publisher","award":["WJ2025Q100"],"award-info":[{"award-number":["WJ2025Q100"]}],"id":[{"id":"10.13039\/501100010846","id-type":"DOI","asserted-by":"publisher"}]},{"name":"University-Industry Collaborative Research Project","award":["2025HX225"],"award-info":[{"award-number":["2025HX225"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>Copper ions (Cu2+) and intracellular biothiols are tightly coupled in cellular redox regulation, where copper\u2013thiol coordination governs oxidative stress and metal homeostasis. However, analytical platforms capable of sequentially monitoring Cu2+ and biothiols within a single molecular system remain scarce. Herein, we report a coumarin-based fluorescent probe XDP that enables sequential ON\u2013OFF\u2013ON sensing of Cu2+ and biothiols through a coordination\u2013competition mechanism. The imine (C=N) site of XDP selectively coordinates Cu2+, leading to fluorescence quenching arising from coordination-induced electronic perturbation and enhanced nonradiative decay. The probe exhibits a linear response toward Cu2+ over 1\u201380 \u03bcM with a detection limit of 0.108 \u03bcM. Subsequent competitive binding of biothiols (GSH, Cys, and Hcy) releases Cu2+ from the complex, thereby restoring fluorescence and enabling detection within 1\u201330 \u03bcM with submicromolar sensitivity. XDP also displays a large Stokes shift (135 nm), which minimizes spectral overlap and improves signal reliability. Notably, Cu2+ binding triggers a distinct color change that supports naked-eye detection and smartphone-based RGB quantification. The probe further enables visualization of Cu2+ and thiol-triggered signal recovery in living cells and zebrafish. This work establishes a versatile analytical platform for probing copper\u2013thiol interactions in environmental and biological systems.<\/jats:p>","DOI":"10.3390\/bios16060351","type":"journal-article","created":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T18:35:05Z","timestamp":1782239705000},"page":"351","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Coumarin-Based Probe for Sequential ON\u2013OFF\u2013ON Detection of Cu2+ and Biothiols: Naked-Eye Detection, Smartphone RGB Readout and In Vivo Imaging"],"prefix":"10.3390","volume":"16","author":[{"given":"Mingjie","family":"Wei","sequence":"first","affiliation":[{"name":"School of Economics and Statistics, Guangzhou University, Guangzhou 510006, China"},{"name":"School of Chemistry and Chemical Engineering, Guangzhou University, Guangzhou 510006, China"},{"name":"School of Pharmacy, Xianning Medical College, Hubei University of Science and Technology, Xianning 437100, China"},{"name":"School of Chemical Engineering and Technology, Zhuhai Campus, Sun Yat-sen University, Zhuhai 519082, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linxin","family":"Zheng","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Xianning Medical College, Hubei University of Science and Technology, Xianning 437100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weilong","family":"Tian","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Xianning Medical College, Hubei University of Science and Technology, Xianning 437100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingfeng","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Xianning Medical College, Hubei University of Science and Technology, Xianning 437100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Xianning Medical College, Hubei University of Science and Technology, Xianning 437100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7845-9397","authenticated-orcid":false,"given":"Lijuan","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering, Guangzhou University, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3652-2903","authenticated-orcid":false,"given":"Li","family":"Niu","sequence":"additional","affiliation":[{"name":"School of Economics and Statistics, Guangzhou University, Guangzhou 510006, China"},{"name":"School of Chemistry and Chemical Engineering, Guangzhou University, Guangzhou 510006, China"},{"name":"School of Chemical Engineering and Technology, Zhuhai Campus, Sun Yat-sen University, Zhuhai 519082, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"14273","DOI":"10.1021\/acs.analchem.2c02690","article-title":"Glass Nanopore Detection of Copper Ions in Single Cells Based on Click Chemistry","volume":"94","author":"Hu","year":"2022","journal-title":"Anal. 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