{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T16:15:50Z","timestamp":1783095350197,"version":"3.54.6"},"reference-count":66,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T00:00:00Z","timestamp":1783036800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program","doi-asserted-by":"publisher","award":["2025YFD2100200"],"award-info":[{"award-number":["2025YFD2100200"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Agricultural Science and Technology Major Project"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>Accurately detecting viable foodborne pathogenic bacteria is essential for food safety risk assessments and public health interventions. Traditional plate counting is time-consuming and operationally cumbersome. Immunological assays are unable to distinguish viable from dead cells, whereas conventional nucleic acid amplification is often affected by residual DNA originating from dead bacteria. These limitations render conventional approaches inadequate for rapid and precise field detection. Functional nucleic acids (FNAs) offer a promising alternative for viability detection because of their high sensitivity, specificity, target diversity, and programmable integrability. This review provides a systematic overview of molecular recognition strategies and FNA-based detection technologies for identifying viable foodborne microorganisms. We categorize the biomarkers targeted by FNAs into nucleic acids, surface structures, and metabolic activities. Building on this categorization, we examine the core principles and technological evolution of primers, aptamers, DNAzymes, guide nucleic acids, and oligonucleotide probes in viability discrimination. We then outline the practical applications of these technologies across the food supply chain and discuss the remaining challenges and future directions in the field. Ultimately, this work provides a theoretical reference and practical guidance for ensuring food safety and advancing precise microbial risk management.<\/jats:p>","DOI":"10.3390\/bios16070364","type":"journal-article","created":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T14:33:06Z","timestamp":1783089186000},"page":"364","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Advances in Detecting Viable\/Dead Foodborne Microorganisms Using Diverse Functional Nucleic Acid-Based Molecular Recognition"],"prefix":"10.3390","volume":"16","author":[{"given":"Yanger","family":"Liu","sequence":"first","affiliation":[{"name":"State Key Laboratory for Quality and Safety of Agro-Products, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huifu","family":"Yuan","sequence":"additional","affiliation":[{"name":"College of Agriculture and Forestry, Hebei North University, Zhangjiakou 075000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Quality and Safety of Agro-Products, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyun","family":"Sun","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Quality and Safety of Agro-Products, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-4768-0879","authenticated-orcid":false,"given":"Peili","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Quality and Safety of Agro-Products, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pazilaiti","family":"Yiming","sequence":"additional","affiliation":[{"name":"Xinjiang Institute of Veterinary Medicine and Feed Supervision, Urumqi 830063, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2203-2823","authenticated-orcid":false,"given":"Ailiang","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Quality and Safety of Agro-Products, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5193-9286","authenticated-orcid":false,"given":"Yanyang","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Quality and Safety of Agro-Products, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"European Food Safety Authority (EFSA), and European Centre for Disease Prevention and Control (ECDC) (2023). The European Union One Health 2022 Zoonoses Report. EFSA J., 21, e8442.","DOI":"10.2903\/j.efsa.2023.8442"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1111\/j.1574-6968.2008.01429.x","article-title":"Novel approaches toward preferential detection of viable cells using nucleic acid amplification techniques","volume":"291","author":"Nocker","year":"2009","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1186\/s40168-017-0285-3","article-title":"Schr\u00f6dinger\u2019s microbes: Tools for distinguishing the living from the dead in microbial ecosystems","volume":"5","author":"Emerson","year":"2017","journal-title":"Microbiome"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.mimet.2013.04.007","article-title":"Comparison of two approaches for identification of haplotypes and point mutations in Candida albicans and Saccharomyces cerevisiae","volume":"94","author":"Larriba","year":"2013","journal-title":"J. Microbiol. Methods"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Law, J.W.F., Ab Mutalib, N.S., Chan, K.G., and Lee, L.H. (2015). Rapid methods for the detection of foodborne bacterial pathogens: Principles, applications, advantages and limitations. Front. Microbiol., 5.","DOI":"10.3389\/fmicb.2014.00770"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/j.biotechadv.2009.12.004","article-title":"An overview of foodborne pathogen detection: In the perspective of biosensors","volume":"28","author":"Velusamy","year":"2010","journal-title":"Biotechnol. Adv."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1948","DOI":"10.1021\/cr030183i","article-title":"Functional Nucleic Acid Sensors","volume":"109","author":"Liu","year":"2009","journal-title":"Chem. Rev."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"5884","DOI":"10.1128\/AEM.01763-14","article-title":"Dead or Alive: Molecular Assessment of Microbial Viability","volume":"80","author":"Cangelosi","year":"2014","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"3540","DOI":"10.1021\/acs.accounts.1c00355","article-title":"Functional Nucleic Acids for Pathogenic Bacteria Detection","volume":"54","author":"Chang","year":"2021","journal-title":"Acc. Chem. Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2881","DOI":"10.1021\/cr400354z","article-title":"From Cascaded Catalytic Nucleic Acids to Enzyme\u2013DNA Nanostructures: Controlling Reactivity, Sensing, Logic Operations, and Assembly of Complex Structures","volume":"114","author":"Wang","year":"2014","journal-title":"Chem. Rev."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"3501","DOI":"10.1002\/bit.28555","article-title":"Research progress in the detection of common foodborne hazardous substances based on functional nucleic acids biosensors","volume":"120","author":"Chen","year":"2023","journal-title":"Biotechnol. Bioeng."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"113286","DOI":"10.1016\/j.foodres.2023.113286","article-title":"Strategy of functional nucleic acids-mediated isothermal amplification for detection of foodborne microbial contaminants: A review","volume":"173","author":"Zhou","year":"2023","journal-title":"Food Res. Int."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"107348","DOI":"10.1016\/j.microc.2022.107348","article-title":"Rapid on-site detection of viable Escherichia coli O157: H7 in lettuce using immunomagnetic separation combined with PMAxx-LAMP and nucleic acid lateral flow strip","volume":"178","author":"Wen","year":"2022","journal-title":"Microchem. J."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Shi, Z., Li, X., Fan, X., Xu, J., Liu, Q., Wu, Z., and Pan, D. (2022). PMA-qPCR method for the selective quantitation of viable lactic acid bacteria in fermented milk. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.984506"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Yang, J., Xu, H., Ke, Z., Kan, N., Zheng, E., Qiu, Y., and Huang, M. (2023). Absolute quantification of viable Vibrio cholerae in seawater samples using multiplex droplet digital PCR combined with propidium monoazide. Front. Microbiol., 14.","DOI":"10.3389\/fmicb.2023.1149981"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"109732","DOI":"10.1016\/j.microc.2023.109732","article-title":"Real-time and visual detection of viable Salmonella in milk from remote pasture via IMS-LAMP-NALFS","volume":"197","author":"Li","year":"2024","journal-title":"Microchem. J."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"133648","DOI":"10.1016\/j.jhazmat.2024.133648","article-title":"A machine vision-assisted argonaute-mediated fluorescence biosensor for the detection of viable Salmonella in food without convoluted DNA extraction and amplification procedures","volume":"466","author":"Zhao","year":"2024","journal-title":"J. Hazard. Mater."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"112906","DOI":"10.1016\/j.bios.2020.112906","article-title":"Light-up RNA aptamer signaling-CRISPR-Cas13a-based mix-and-read assays for profiling viable pathogenic bacteria","volume":"176","author":"Zhang","year":"2021","journal-title":"Biosens. Bioelectron."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1670","DOI":"10.1021\/acs.jafc.1c07182","article-title":"Isothermal RNA amplification for the detection of viable pathogenic bacteria to estimate the Salmonella virulence for causing enteritis","volume":"70","author":"Xue","year":"2022","journal-title":"J. Agric. Food Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"340885","DOI":"10.1016\/j.aca.2023.340885","article-title":"Sensitive detection of viable Salmonella bacteria based on tertiary cascade signal amplification via splintR ligase ligation-PCR amplification-CRISPR\/Cas12a cleavage","volume":"1248","author":"Zhou","year":"2023","journal-title":"Anal. Chim. Acta"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"114282","DOI":"10.1016\/j.bios.2022.114282","article-title":"Aptamer-based Cas14a1 biosensor for amplification-free live pathogenic detection","volume":"211","author":"Wei","year":"2022","journal-title":"Biosens. Bioelectron."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"139132","DOI":"10.1016\/j.snb.2025.139132","article-title":"Magnetic nanoconfined metabolic biosensing for rapid and high-sensitivity detecting viable E. coli O157:H7 in aquatic matrices","volume":"449","author":"Zhang","year":"2026","journal-title":"Sens. Actuators B Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"135365","DOI":"10.1016\/j.jhazmat.2024.135365","article-title":"An emerging assay for rapid diagnosis of live Salmonella Typhimurium by exploiting aqueous\/liquid crystal interface","volume":"477","author":"Mehrzad","year":"2024","journal-title":"J. Hazard. Mater."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"116715","DOI":"10.1016\/j.bios.2024.116715","article-title":"Endoprotein-activating DNAzyme assay for nucleic acid extraction- and amplification-free detection of viable pathogenic bacteria","volume":"266","author":"Wu","year":"2024","journal-title":"Biosens. Bioelectron."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"117503","DOI":"10.1016\/j.bios.2025.117503","article-title":"DNAzyme-driven Z-scheme g-C3N4\/V2C heterojunction reactivation for photoelectrochemical assessment of bacterial viability","volume":"282","author":"Deng","year":"2025","journal-title":"Biosens. Bioelectron."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"110711","DOI":"10.1016\/j.foodcont.2024.110711","article-title":"Rapid and accurate AuNPs-sodium deoxycholate-propidium monoazide-qPCR technique for simultaneous detection of viable Listeria monocytogenes and Salmonella","volume":"166","author":"Huang","year":"2024","journal-title":"Food Control"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Liu, Y., Wei, C., Wan, H., Liang, X., Jiang, T., Dong, Y., Zhao, X., and Zhong, T. (2023). Preliminary study on rapid and simultaneous detection of viable Escherichia coli O157:H7, Staphylococcus aureus, and Salmonella by PMA-mPCR in food. Molecules, 28.","DOI":"10.3390\/molecules28155835"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Hu, L., Fu, Y., Zhang, S., Pan, Z., Xia, J., Zhu, P., and Guo, J. (2022). An assay combining droplet digital PCR with propidium monoazide treatment for the accurate detection of live cells of Vibrio vulnificus in plasma samples. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.927285"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Zhang, X., Liu, C., Kong, M., He, Z., Cao, Z., and Jin, D. (2026). Development of PMAxx-droplet digital PCR method for the absolute quantification of viable Shigella flexneri and Shigella sonnei strains in water. Front. Microbiol., 17.","DOI":"10.3389\/fmicb.2026.1769049"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"e04002","DOI":"10.1128\/spectrum.04002-25","article-title":"Comparative evaluation of viability PCR reagents highlights the superior efficacy of PMAxx azo dye for bacterial and viral viability discrimination using real-time and digital PCR","volume":"14","author":"Hongsibsam","year":"2026","journal-title":"Microbiol. Spectr."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"8910","DOI":"10.1021\/acs.analchem.5c06212","article-title":"Rapid and Sensitive Pathogen Detection and Viability Evaluation: A Lateral Flow Assay Based on One-to-Multiple Signal Amplification","volume":"98","author":"Liu","year":"2026","journal-title":"Anal. Chem."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Feng, X., Zhou, D., Gan, B., Xie, G., and Xu, H. (2022). A combination of novel nucleic acid cross-linking dye and recombinase-aided amplification for the rapid detection of viable Salmonella in milk. Foods, 11.","DOI":"10.3390\/foods11152375"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"4895","DOI":"10.3168\/jds.2022-21900","article-title":"A novel photoreactive DNA-binding dye for detecting viable Klebsiella pneumoniae in powdered infant formula","volume":"105","author":"Feng","year":"2022","journal-title":"J. Dairy Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1372","DOI":"10.1039\/D4TB02178A","article-title":"A phage amplification-assisted SEA-CRISPR\/Cas12a system for viable bacteria detection","volume":"13","author":"Xiao","year":"2025","journal-title":"J. Mater. Chem. B"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"107360","DOI":"10.1016\/j.cclet.2022.03.083","article-title":"A portable viable Salmonella detection device based on microfluidic chip and recombinase aided amplification","volume":"34","author":"Qi","year":"2023","journal-title":"Chin. Chem. Lett."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.aca.2018.02.009","article-title":"Biotin-exposure-based immunomagnetic separation coupled with nucleic acid lateral flow biosensor for visibly detecting viable Listeria monocytogenes","volume":"1017","author":"Li","year":"2018","journal-title":"Anal. Chim. Acta"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"114594","DOI":"10.1016\/j.microc.2025.114594","article-title":"Vancomycin-functionalized magnetic bead-qPCR platform for rapid differentiation of viable and dead Listeria monocytogenes in food samples","volume":"216","author":"Yuan","year":"2025","journal-title":"Microchem. J."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"105492","DOI":"10.1016\/j.tifs.2025.105492","article-title":"Phage-based biosensors: From recognition to signal transduction empowered by amplification strategies for detecting foodborne pathogens","volume":"168","author":"Li","year":"2026","journal-title":"Trends Food Sci. Technol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"11013","DOI":"10.1021\/acs.analchem.9b01263","article-title":"Paper-based all-in-one origami microdevice for nucleic acid amplification testing for rapid colorimetric identification of live cells for point-of-care testing","volume":"91","author":"Trieu","year":"2019","journal-title":"Anal. Chem."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"109494","DOI":"10.1016\/j.foodcont.2022.109494","article-title":"Lateral flow biosensor based on LAMP-CRISPR\/Cas12a for sensitive and visualized detection of Salmonella spp.","volume":"145","author":"Lee","year":"2023","journal-title":"Food Control"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2783","DOI":"10.1021\/acs.analchem.5c05423","article-title":"A Reusable Dual-Mode Aptasensor Based on Bipolar Electrode for Sensitive Detection of Live Clostridium perfringens","volume":"98","author":"Yang","year":"2026","journal-title":"Anal. Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"116731","DOI":"10.1016\/j.trac.2022.116731","article-title":"Progress and challenges in bacterial whole-cell-components Aptamer advanced screening and site identification","volume":"157","author":"Su","year":"2022","journal-title":"TrAC Trends Anal. Chem."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Pham, T.T., Huyen, T.T., Hong Oanh, N., Dai Tran, L., Tran, L., Truong, H.V., and Thi Phuong Trang, T.N.L. (2025). A Novel Aptamer Selection Strategy for Pseudomonas aeruginosa and Its Application as a Detecting Probe in a Hybrid Lateral Flow Assay. Molecules, 30.","DOI":"10.20944\/preprints202507.1320.v1"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"15997","DOI":"10.1039\/C9RA10733A","article-title":"Isolation ssDNA aptamers specific for both live and viable but nonculturable state Vibrio vulnificus using whole bacteria-SEILEX technology","volume":"10","author":"Liu","year":"2020","journal-title":"RSC Adv."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"116013","DOI":"10.1016\/j.bios.2024.116013","article-title":"Selection and application of highly specific Salmonella Typhimurium aptamers against matrix interference","volume":"249","author":"Du","year":"2024","journal-title":"Biosens. Bioelectron."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2982","DOI":"10.7150\/thno.19374","article-title":"Discovery of Aptamer Ligands for Hepatic Stellate Cells Using SELEX","volume":"7","author":"Chen","year":"2017","journal-title":"Theranostics"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Sordyl, D., Cielesz, J., Bohdan, D.R., Baulin, E.F., and Bujnicki, J.M. (2026). ARTEM server: An online tool for nucleic acid 3D motif searches, 3D structure superposition and structure-based alignment. Nucleic Acids Res., gkag428.","DOI":"10.1093\/nar\/gkag428"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"108461","DOI":"10.1016\/j.biotechadv.2024.108461","article-title":"Design and application of microfluidics in aptamer SELEX and Aptasensors","volume":"77","author":"Zhang","year":"2024","journal-title":"Biotechnol. Adv."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"117268","DOI":"10.1016\/j.microc.2026.117268","article-title":"Aptamer-based CRISPR\/Cas12a system for amplification-free live pathogenic Shigella flexneri detection","volume":"222","author":"Geng","year":"2026","journal-title":"Microchem. J."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"100318","DOI":"10.1016\/j.snr.2025.100318","article-title":"Nucleic acid-free aptamer-CRISPR\/Cas14 biosensor for prosthetic joint infection rapid detection","volume":"9","author":"Geng","year":"2025","journal-title":"Sens. Actuators Rep."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"168309","DOI":"10.1016\/j.cej.2025.168309","article-title":"Simultaneous capture, inactivation, and detection of total\/dead\/live S. Typhimurium using SERS\/ATP-bioluminescence dual-mode immune biosensors","volume":"523","author":"Xie","year":"2025","journal-title":"Chem. Eng. J."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"117566","DOI":"10.1016\/j.bios.2025.117566","article-title":"Plasma-based ultrasensitive detection of Mycobacterium tuberculosis ESAT6\/CFP10 fusion antigen using a CRISPR-driven aptamer fluorescence testing (CRAFT)","volume":"284","author":"Liu","year":"2025","journal-title":"Biosens. Bioelectron."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"11211","DOI":"10.1021\/acs.analchem.3c00603","article-title":"Ultrasensitive and specific phage@DNAzyme probe-triggered fluorescent click chemistry for on-site detection of foodborne pathogens using a smartphone","volume":"95","author":"You","year":"2023","journal-title":"Anal. Chem."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"11951","DOI":"10.1021\/acs.analchem.4c01757","article-title":"Hook-Like DNAzyme-Activated Autocatalytic Biosensor for the Universal Detection of Pathogenic Bacteria","volume":"96","author":"Liu","year":"2024","journal-title":"Anal. Chem."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"28942","DOI":"10.1038\/s41598-024-80392-4","article-title":"Colorimetric and electrochemical analysis of DNAzyme-LAMP amplicons for the detection of Escherichia coli in food matrices","volume":"14","author":"Sewid","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"12488","DOI":"10.1021\/acs.jafc.5c01068","article-title":"Preamplification-free detection of viable microorganisms in fermentation using tandem CRISPR nuclease probe","volume":"73","author":"Xu","year":"2025","journal-title":"J. Agric. Food Chem."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"17941","DOI":"10.1021\/acs.analchem.4c02204","article-title":"A propidium monoazide-assisted digital CRISPR\/Cas12a assay for selective detection of live bacteria in sample","volume":"96","author":"Yin","year":"2024","journal-title":"Anal. Chem."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"4659","DOI":"10.1039\/D4LC00672K","article-title":"Fast and sensitive detection of viable Escherichia coli O157:H7 using a microwell-confined and propidium monoazide-assisted digital CRISPR microfluidic platform","volume":"24","author":"Yin","year":"2024","journal-title":"Lab A Chip"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"18037","DOI":"10.1021\/acs.jafc.3c06530","article-title":"DNA Extraction- and Amplification-Free Nucleic Acid Biosensor for the Detection of Foodborne Pathogens Based on CRISPR\/Cas12a and Argonaute Protein-Mediated Cascade Signal Amplification","volume":"71","author":"Chen","year":"2023","journal-title":"J. Agric. Food Chem."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"118446","DOI":"10.1016\/j.bios.2026.118446","article-title":"Dual CRISPR\/Cas-driven amplification-free surface-enhanced Raman scattering biosensor combined with a smartphone for simultaneous detection of total and live target bacteria","volume":"299","author":"Jiao","year":"2026","journal-title":"Biosens. Bioelectron."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"135543","DOI":"10.1016\/j.snb.2024.135543","article-title":"DNA and ATP synergistically triggered argonaute-mediated sensor for the ultrasensitive detection of viable Salmonella without DNA extraction and amplification","volume":"408","author":"Wang","year":"2024","journal-title":"Sens. Actuators B Chem."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"e202508870","DOI":"10.1002\/anie.202508870","article-title":"In-field molecular diagnostics of plant pathogens using bioluminescent CRISPR-guided caspase assay","volume":"64","author":"Hu","year":"2025","journal-title":"Angew. Chem. Int. Ed."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"2293","DOI":"10.3168\/jds.2025-27506","article-title":"Rapid and simultaneous quantification of viable Lactobacillus and Streptococcus thermophilus cells in yogurt by flow cytometry combined with novel oligonucleotide probes","volume":"109","author":"Cai","year":"2026","journal-title":"J. Dairy Sci."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"117290","DOI":"10.1016\/j.lwt.2024.117290","article-title":"Rapid and accurate flow cytometric enumeration of viable Listeria monocytogenes in beef via propidium monoazide and fluorescent molecular probe","volume":"216","author":"Liu","year":"2025","journal-title":"LWT Food Sci. Technol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1021\/acs.analchem.3c03742","article-title":"Rapid and accurate quantification of viable lactobacillus cells in infant formula by flow cytometry combined with propidium monoazide and signal-enhanced fluorescence in situ hybridization","volume":"96","author":"Wang","year":"2024","journal-title":"Anal. Chem."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"115477","DOI":"10.1016\/j.lwt.2023.115477","article-title":"Rapid and accurate quantification of viable Bacillus cereus in foods with a propidium monoazide (PMA)\u2014Fluorescence in situ hybridization (FISH)\u2014Flow cytometry (FCM) method","volume":"188","author":"Bai","year":"2023","journal-title":"LWT Food Sci. 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