{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T23:57:43Z","timestamp":1792799863108,"version":"4.5.0"},"reference-count":119,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T00:00:00Z","timestamp":1784592000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22104157"],"award-info":[{"award-number":["22104157"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22274169"],"award-info":[{"award-number":["22274169"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["22104157"],"award-info":[{"award-number":["22104157"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"award":["22274169"],"award-info":[{"award-number":["22274169"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021171","name":"Guangdong Basic and Applied Basic Research Foundation","doi-asserted-by":"crossref","award":["2023A151511099"],"award-info":[{"award-number":["2023A151511099"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Shenzhen Science and Technology Program","award":["RCBS20210706092410027"],"award-info":[{"award-number":["RCBS20210706092410027"]}]},{"name":"Shenzhen Science and Technology Program","award":["JCYJ20250604175557073"],"award-info":[{"award-number":["JCYJ20250604175557073"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>Pathogenic bacterial infections remain a persistent global public health crisis. However, traditional clinical detection methods\u2014such as culture-based assays and polymerase chain reaction (PCR)\u2014are often time-consuming and labor-intensive, and they lack sufficient sensitivity for low-abundance pathogens, hindering rapid point-of-care diagnosis. With label-free, ultra-sensitive molecular fingerprinting, Surface-Enhanced Raman Scattering (SERS) has emerged as a powerful tool for rapid pathogen identification. This review summarizes the evolution of SERS-based bacterial detection from fundamental nanomaterials to integrated clinical diagnostic platforms. The article explores four core dimensions: functional integration and enrichment strategies of colloidal probes; structural design and multifaceted capture mechanisms of solid substrates; synergistic advantages of microfluidic systems in enabling automated \u201csample-to-answer\u201d architectures; and the translational potential of SERS-based lateral flow assays (LFAs) for robust point-of-care testing (POCT). This review reveals a research shift from maximizing electromagnetic enhancement toward overcoming matrix effects in clinical samples and ensuring robustness. In synergy with microfluidics, LFAs, and AI, SERS technology is bridging the bench-to-bedside gap, offering a roadmap for next-generation decentralized, high-precision diagnostics.<\/jats:p>","DOI":"10.3390\/bios16070396","type":"journal-article","created":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T15:17:52Z","timestamp":1784647072000},"page":"396","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A Review of SERS-Based Bacterial Detection from Nanomaterials to Integrated Clinical Platforms"],"prefix":"10.3390","volume":"16","author":[{"given":"Yueqi","family":"Yang","sequence":"first","affiliation":[{"name":"School of Biomedical Engineering, Shenzhen Campus, Sun Yat-sen University, Shenzhen 518107, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Li","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering, Shenzhen Campus, Sun Yat-sen University, Shenzhen 518107, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyi","family":"Hu","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering, Shenzhen Campus, Sun Yat-sen University, Shenzhen 518107, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zong","family":"Dai","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering, Shenzhen Campus, Sun Yat-sen University, Shenzhen 518107, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0486-5437","authenticated-orcid":false,"given":"Jianhe","family":"Guo","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering, Shenzhen Campus, Sun Yat-sen University, Shenzhen 518107, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1038\/s41377-024-01718-5","article-title":"In Vivo Surface-Enhanced Raman Scattering Techniques: Nanoprobes, Instrumentation, and Applications","volume":"14","author":"Chang","year":"2025","journal-title":"Light Sci. 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