{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T04:47:13Z","timestamp":1785646033165,"version":"3.56.0"},"reference-count":40,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T00:00:00Z","timestamp":1785456000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>Microfluidic cell culture systems provide controlled microscale environments for biomedical research; however, cell viability within closed microchambers depends on adequate oxygen availability during the adhesion phase and on the mechanical stresses generated after perfusion begins. Experimental characterization of oxygen depletion and local shear stress remains challenging due to the small dimensions involved and the complexity of transport phenomena. In this study, a computational framework was developed to assess oxygen transport and hydrodynamic shear stress in an SU-8-based microfluidic culture chamber. Oxygen diffusion and cellular consumption were first modeled under static conditions to determine cell survival time prior to perfusion. The influence of chamber height on oxygen availability was investigated, and an empirical correlation was derived to predict oxygen concentration as a function of chamber geometry. Subsequently, medium perfusion was introduced, and the resulting wall shear stresses acting on adhered cells were evaluated under different flow conditions. The simulations demonstrated that chamber height significantly affects oxygen depletion time, while both chamber geometry and flow rate influence the magnitude of wall shear stress. The proposed framework provides practical design guidelines for optimizing microfluidic culture systems, enabling adequate oxygen supply and physiologically compatible mechanical conditions while reducing reliance on extensive experimental testing.<\/jats:p>","DOI":"10.3390\/bios16080414","type":"journal-article","created":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T08:20:18Z","timestamp":1785486018000},"page":"414","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Computational Assessment of Oxygen Availability and Shear Stress in Microfluidic Cell Culture Chambers for Optimized Cell Adhesion"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-6689-9143","authenticated-orcid":false,"given":"Mahdi","family":"Poursaberi","sequence":"first","affiliation":[{"name":"School of Engineering and Architecture (EINA), University of Zaragoza, 50018 Zaragoza, Spain"},{"name":"Aragon Institute of Engineering Research (I3A), University of Zaragoza, 50018 Zaragoza, Spain"},{"name":"Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7802-3411","authenticated-orcid":false,"given":"Guillermo","family":"Hauke","sequence":"additional","affiliation":[{"name":"School of Engineering and Architecture (EINA), University of Zaragoza, 50018 Zaragoza, Spain"},{"name":"Aragon Institute of Engineering Research (I3A), University of Zaragoza, 50018 Zaragoza, Spain"},{"name":"Fluid Mechanics Department, University of Zaragoza, 50018 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0509-1450","authenticated-orcid":false,"given":"S. Jamaleddin","family":"Mousavi","sequence":"additional","affiliation":[{"name":"Medtronic, 01600 Trevoux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0088-7222","authenticated-orcid":false,"given":"Mohamed H.","family":"Doweidar","sequence":"additional","affiliation":[{"name":"School of Engineering and Architecture (EINA), University of Zaragoza, 50018 Zaragoza, Spain"},{"name":"Aragon Institute of Engineering Research (I3A), University of Zaragoza, 50018 Zaragoza, Spain"},{"name":"Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1038\/nbt.2989","article-title":"Microfluidic organs-on-chips","volume":"32","author":"Bhatia","year":"2014","journal-title":"Nat. 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