{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T12:16:36Z","timestamp":1789042596020,"version":"build-2803163510"},"reference-count":33,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T00:00:00Z","timestamp":1788998400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32402314"],"award-info":[{"award-number":["32402314"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["32402314"],"award-info":[{"award-number":["32402314"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests","award":["SKLOF202502"],"award-info":[{"award-number":["SKLOF202502"]}]},{"award":["SKLOF202502"],"award-info":[{"award-number":["SKLOF202502"]}],"id":[{"id":"https:\/\/ror.org\/04bcwdq54","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>Mulberry mosaic dwarf-associated virus (MMDaV), a member of the genus Mulcrilevirus in the family Geminiviridae, poses a serious threat to mulberry cultivation and growth in China. Early and accurate diagnosis is a prerequisite for the effective prevention and control of MMDaV-related disease. In this study, a loop-mediated isothermal amplification (LAMP)-based detection method was established for the specific identification of MMDaV. Three pairs of specific primers were designed targeting the nucleotide sequences of the MMDaV V2 gene. The optimized LAMP reaction was performed at a constant temperature of 65 \u00b0C for 60 min, and the established method exhibited high specificity with no cross-reactivity observed against other tested geminiviruses. The LAMP method achieved a detection limit of 200 pg of target MMDaV DNA, which was tenfold more sensitive than conventional PCR. Furthermore, the amplification results could be directly visualized via distinct color change. Collectively, the developed LAMP method enables efficient, sensitive, and specific isothermal detection of MMDaV and holds great promise for rapid diagnosis of mulberry viral disease.<\/jats:p>","DOI":"10.3390\/bios16090509","type":"journal-article","created":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T11:38:39Z","timestamp":1789040319000},"page":"509","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Rapid and Sensitive Loop-Mediated Isothermal Amplification Assay for the Detection of Mulberry Mosaic Dwarf-Associated Virus"],"prefix":"10.3390","volume":"16","author":[{"given":"Shaoshuang","family":"Sun","sequence":"first","affiliation":[{"name":"Shandong Key Laboratory of Wetland Ecology and Biodiversity Conservation in the Lower Yellow River, School of Life Sciences, Qufu Normal University, Qufu 273165, China"},{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5311-7331","authenticated-orcid":false,"given":"Xueping","family":"Zhou","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China"},{"name":"State Key Laboratory of Rice Biology and Breeding, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"}]},{"given":"Xiuling","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Jones, R.A.C. 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