{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T09:50:39Z","timestamp":1790502639956,"version":"4.1.0"},"reference-count":57,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2015,6,15]],"date-time":"2015-06-15T00:00:00Z","timestamp":1434326400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Swiss National Science Foundation","doi-asserted-by":"publisher","award":["200021-152897"],"award-info":[{"award-number":["200021-152897"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"name":"US-NSF Triangle MRSEC for Programmable Soft Matter","award":["DMR-1121107"],"award-info":[{"award-number":["DMR-1121107"]}]},{"name":"NSF-ASSIST Nanosystems Engineering Research Center","award":["EEC-1160483"],"award-info":[{"award-number":["EEC-1160483"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>An alternative current (AC) dielectrophoretic lab-on-chip setup was evaluated  as a rapid tool of capture and assembly of microalga Chlamydomonas reinhardtii in  two-dimensional (2D) close-packed arrays. An electric field of 100 V\u00b7cm\u22121, 100 Hz applied for 30 min was found optimal to collect and assemble the algae into single-layer structures of closely packed cells without inducing cellular oxidative stress. Combined with oxidative stress specific staining and fluorescence microscopy detection, the capability of using the 2D whole-cell assembly on-chip to follow the reactive oxygen species (ROS) production  and oxidative stress during short-term exposure to several environmental contaminants, including mercury, methylmercury, copper, copper oxide nanoparticles (CuO-NPs), and diuron was explored. The results showed significant increase of the cellular ROS when  C. reinhardtii was exposed to high concentrations of methylmercury, CuO-NPs, and  10\u22125 M Cu. Overall, this study demonstrates the potential of combining AC-dielectrophoretically assembled two-dimensional algal structures with cell metabolic analysis using fluorescence staining, as a rapid analytical tool for probing the effect of contaminants in highly  impacted environment.<\/jats:p>","DOI":"10.3390\/bios5020319","type":"journal-article","created":{"date-parts":[[2015,6,15]],"date-time":"2015-06-15T10:57:58Z","timestamp":1434365878000},"page":"319-336","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Two-Dimensional Algal Collection and Assembly by Combining AC-Dielectrophoresis with Fluorescence Detection for Contaminant-Induced Oxidative Stress Sensing"],"prefix":"10.3390","volume":"5","author":[{"given":"Coralie","family":"Siebman","sequence":"first","affiliation":[{"name":"Environmental Biogeochemistry and Ecotoxicology, Institute F.-A. 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Saf."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"605","DOI":"10.3109\/17435390.2013.809810","article-title":"Oxidative stress induced by inorganic nanoparticles in bacteria and aquatic microalgae\u2014State of the art and knowledge gaps","volume":"8","author":"Slaveykova","year":"2014","journal-title":"Nanotoxicology"},{"key":"ref_3","first-page":"1660","article-title":"Cell injury and death: Oxidative stress and antioxidant defense system: Review","volume":"29","author":"Esrefoglu","year":"2009","journal-title":"Turk. Klin. Tip Bilim."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1016\/S0141-1136(02)00146-0","article-title":"Oxidative stress in ecotoxicology: From the analysis of individual antioxidants to a more integrated approach","volume":"54","author":"Regoli","year":"2002","journal-title":"Mar. Environ. Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1016\/S1360-1385(02)02312-9","article-title":"Oxidative stress, antioxidants and stress tolerance","volume":"7","author":"Mittler","year":"2002","journal-title":"Trends Plant Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1111\/j.1529-8817.2009.00663.x","article-title":"Metal-induced reactive oxygen species production in Chlamydomonas reinhardtii (chlorophyceae)","volume":"45","author":"Szivak","year":"2009","journal-title":"J. Phycol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1264\/jsme2.ME11126","article-title":"Oxidative stress induced in microorganisms by zero-valent iron nanoparticles","volume":"26","author":"Sevcu","year":"2011","journal-title":"Microbes Environ."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1897\/06-1636R.1","article-title":"Flow cytometry as a tool to study phytotoxic modes of action","volume":"26","author":"Adler","year":"2007","journal-title":"Environ. Toxicol. Chem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1016\/j.plaphy.2010.08.016","article-title":"Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants","volume":"48","author":"Gill","year":"2010","journal-title":"Plant Physiol. Biochem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.aquatox.2014.07.010","article-title":"Antagonistic and synergistic effects of light irradiation on the effects of copper on Chlamydomonas reinhardtii","volume":"155","author":"Cheloni","year":"2014","journal-title":"Aquat. Toxicol."},{"key":"ref_11","unstructured":"Jerome, O.N. (2011). Encyclopedia of Environmental Health, Elsevier."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1021\/tx400410s","article-title":"Systems toxicology: From basic research to risk assessment","volume":"27","author":"Sturla","year":"2014","journal-title":"Chem. Res. Toxicol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1016\/j.bbagen.2013.03.034","article-title":"Detection and characterisation of radicals in biological materials using epr methodology","volume":"1840","author":"Hawkins","year":"2014","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/B978-0-12-405883-5.00006-5","article-title":"Electrochemical biosensors for on-chip detection of oxidative stress from cells","volume":"526","author":"Enomoto","year":"2013","journal-title":"Method Enzymol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1016\/j.bbagen.2013.05.004","article-title":"The challenges of using fluorescent probes to detect and quantify specific reactive oxygen species in living cells","volume":"1840","author":"Winterbourn","year":"2014","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1016\/j.bbagen.2013.06.030","article-title":"In vitro measurements and interpretation of total antioxidant capacity","volume":"1840","author":"Fraga","year":"2014","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/j.bios.2014.12.044","article-title":"Portable oxidative stress sensor: Dynamic and non-invasive measurements of extracellular H2O2 released by algae","volume":"68","author":"Koman","year":"2015","journal-title":"Biosens. Bioelectron."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Suarez, G., Santschi, C., Slaveykova, V.I., and Martin, O.J.F. (2013). Sensing the dynamics of oxidative stress using enhanced absorption in protein-loaded random media. Sci. Rep., 3.","DOI":"10.1038\/srep03447"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/j.bios.2012.10.083","article-title":"Biosensor based on chemically-designed anchorable cytochrome c for the detection of H2O2 released by aquatic cells","volume":"42","author":"Suarez","year":"2013","journal-title":"Biosens. Bioelectron."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1007\/s00216-011-5107-z","article-title":"Micro-algal biosensors","volume":"401","author":"Brayner","year":"2011","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1051\/epjap:2006112","article-title":"Algal biosensors for aquatic ecosystems monitoring","volume":"36","author":"Durrieu","year":"2006","journal-title":"Eur. Phys. J. Appl. Phys."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1007\/s00216-010-4084-y","article-title":"Whole-cell aquatic biosensors","volume":"400","author":"Eltzov","year":"2011","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1785","DOI":"10.1007\/s00216-011-5364-x","article-title":"Staying alive: New perspectives on cell immobilization for biosensing purposes","volume":"402","author":"Michelini","year":"2012","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.copbio.2005.12.005","article-title":"Advances in preservation methods: Keeping biosensor microorganisms alive and active","volume":"17","author":"Bjerketorp","year":"2006","journal-title":"Curr. Opin. Biotechnol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1015","DOI":"10.1016\/S0956-5663(02)00220-8","article-title":"A bioluminescent sensor for high throughput toxicity classification","volume":"18","author":"Kim","year":"2003","journal-title":"Biosens. Bioelectron."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1433","DOI":"10.1016\/j.bios.2004.04.022","article-title":"Aqueous sol-gel encapsulation of genetically engineered Moraxella spp. cells for the detection of organophosphates","volume":"20","author":"Yu","year":"2005","journal-title":"Biosens. Bioelectron."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1007\/s00604-008-0017-2","article-title":"A self-assembled monolayers based conductometric algal whole cell biosensor for water monitoring","volume":"163","author":"Guedri","year":"2008","journal-title":"Microchim. Acta"},{"key":"ref_28","first-page":"87","article-title":"Microbial fermentations with immobilized cells","volume":"77","author":"Ramakrishna","year":"1999","journal-title":"Curr. Sci. India"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Bickerstaff, G.F. (1997). Immobilization of Enzymes and Cells, Humana Press.","DOI":"10.1385\/0896033864"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Pethig, R. (2010). Dielectrophoresis: Status of the theory, technology, and applications. Biomicrofluidics, 4.","DOI":"10.1063\/1.3456626"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2331","DOI":"10.1002\/elps.201100107","article-title":"Dielectrophoretic monitoring of microorganisms in environmental applications","volume":"32","year":"2011","journal-title":"Electrophoresis"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2338","DOI":"10.1088\/0022-3727\/31\/18\/021","article-title":"AC electrokinetics: A review of forces in microelectrode structures","volume":"31","author":"Ramos","year":"1998","journal-title":"J. Phys. D Appl. Phys."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Cheng, I.F., Chang, H.C., Hou, D., and Chang, H.C. (2007). An integrated dielectrophoretic chip for continuous bioparticle filtering, focusing, sorting, trapping, and detecting. Biomicrofluidics, 1.","DOI":"10.1063\/1.2723669"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1800","DOI":"10.1016\/j.bios.2010.09.022","article-title":"Dielectrophoretic platforms for biomicrofluidic systems","volume":"26","author":"Khoshmanesh","year":"2011","journal-title":"Biosens. Bioelectron."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1039\/b110990c","article-title":"Microsample preparation by dielectrophoresis: Isolation of malaria","volume":"2","author":"Gascoyne","year":"2002","journal-title":"Lab Chip"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Suscillon, C., Velev, O.D., and Slaveykova, V.I. (2013). Alternating current-dielectrophoresis driven on-chip collection and chaining of green microalgae in freshwaters. Biomicrofluidics, 7.","DOI":"10.1063\/1.4801870"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1039\/B309131G","article-title":"Water quality test based on dielectrophoretic measurements of fresh water algae Selenastrum capricornutum","volume":"5","author":"Hubner","year":"2003","journal-title":"J. Environ. Monit."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1039\/b717850f","article-title":"On-chip electric field driven assembly of biocomposites from live cells and functionalized particles","volume":"4","author":"Gupta","year":"2008","journal-title":"Soft Matter"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"3441","DOI":"10.1021\/la902989r","article-title":"On-chip dielectrophoretic coassembly of live cells and particles into responsive biomaterials","volume":"26","author":"Gupta","year":"2010","journal-title":"Langmuir"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"952","DOI":"10.1002\/cyto.a.22338","article-title":"Optimization of the C11-BODIPY581\/591 dye for the determination of lipid oxidation in Chlamydomonas reinhardtii by flow cytometry","volume":"83","author":"Cheloni","year":"2013","journal-title":"Cytom. A"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.1083\/jcb.200903097","article-title":"Accuracy and precision in quantitative fluorescence microscopy","volume":"185","author":"Waters","year":"2009","journal-title":"J. Cell Biol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH image to ImageJ: 25 years of image analysis","volume":"9","author":"Schneider","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1315","DOI":"10.1039\/b706559k","article-title":"A microfluidic device for continuous capture and concentration of microorganisms from potable water","volume":"7","author":"Balasubramanian","year":"2007","journal-title":"Lab Chip"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Unni, H.N., Hartono, D., Yung, L.Y.L., Ng, M.M.L., Lee, H.P., Khoo, B.C., and Lim, K.M. (2012). Characterization and separation of cryptosporidium and giardia cells using on-chip dielectrophoresis. Biomicrofluidics, 6.","DOI":"10.1063\/1.3671065"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2658","DOI":"10.1002\/1522-2683(200208)23:16<2658::AID-ELPS2658>3.0.CO;2-O","article-title":"Trapping of DNA by dielectrophoresis","volume":"23","author":"Asbury","year":"2002","journal-title":"Electrophoresis"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1007\/s10404-011-0900-8","article-title":"On-chip separation of lactobacillus bacteria from yeasts using dielectrophoresis","volume":"12","author":"Khoshmanesh","year":"2012","journal-title":"Microfluid. Nanofluidics"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1016\/j.bios.2003.08.013","article-title":"Dielectrophoretic registration of living cells to a microelectrode assembly","volume":"19","author":"Gray","year":"2004","journal-title":"Biosens. Bioelectron."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"39","DOI":"10.2144\/000113802","article-title":"Spatial manipulation of cells and organelles using single electrode dielectrophoresis","volume":"52","author":"Graham","year":"2012","journal-title":"Biotechniques"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Yang, L., Banada, P.P., Bhunia, A.K., and Bashir, R. (2008). Effects of dielectrophoresis on growth, viability and immuno-reactivity of listeria monocytogenes. J. Biol. Eng., 2.","DOI":"10.1186\/1754-1611-2-6"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1002\/elps.201200292","article-title":"Metabolic viability of Escherichia coli trapped by dielectrophoresis in microfluidics","volume":"34","author":"Donato","year":"2013","journal-title":"Electrophoresis"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"6424","DOI":"10.1021\/es072037q","article-title":"Mixture toxicity of three photosystem II inhibitors (atrazine, isoproturon, and diuron) toward photosynthesis of freshwater phytoplankton studied in outdoor mesocosms","volume":"42","author":"Knauert","year":"2008","journal-title":"Environ. Sci. Technol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1007\/s00216-010-3962-7","article-title":"Profiling of the reactive oxygen species-related ecotoxicity of CuO, ZnO, TiO2, silver and fullerene nanoparticles using a set of recombinant luminescent Escherichia coli strains: Differentiating the impact of particles and solubilised metals","volume":"398","author":"Ivask","year":"2010","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/j.aquatox.2015.02.010","article-title":"Dynamics of sub-lethal effects of nano-CuO on the microalga Chlamydomonas reinhardtii during short-term exposure","volume":"161","author":"Maillard","year":"2015","journal-title":"Aquat. Toxicol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1016\/j.aquatox.2013.09.015","article-title":"Evaluation of toxicity and oxidative stress induced by copper oxide nanoparticles in the green alga Chlamydomonas reinhardtii","volume":"142","author":"Melegari","year":"2013","journal-title":"Aquat. Toxicol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/S0048-3575(02)00009-3","article-title":"Effect of oxyfluorfen and diuron alone and in mixture on antioxidative enzymes of scenedesmus obliquus","volume":"72","author":"Geoffroy","year":"2002","journal-title":"Pestic. Biochem. Phys."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1200","DOI":"10.1016\/j.ecoenv.2009.01.003","article-title":"Oxidative stress and antioxidant defenses in two green microalgae exposed to copper","volume":"72","author":"Sabatini","year":"2009","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1285","DOI":"10.1007\/s10646-010-0514-z","article-title":"Mercury-induced oxidative stress and impact on antioxidant enzymes in Chlamydomonas reinhardtii","volume":"19","author":"Elbaz","year":"2010","journal-title":"Ecotoxicology"}],"container-title":["Biosensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6374\/5\/2\/319\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T20:47:53Z","timestamp":1760215673000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6374\/5\/2\/319"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,6,15]]},"references-count":57,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2015,6]]}},"alternative-id":["bios5020319"],"URL":"https:\/\/doi.org\/10.3390\/bios5020319","relation":{},"ISSN":["2079-6374"],"issn-type":[{"value":"2079-6374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,6,15]]}}}