{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T04:09:12Z","timestamp":1791000552219,"version":"4.1.0"},"reference-count":30,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2018,4,2]],"date-time":"2018-04-02T00:00:00Z","timestamp":1522627200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>The vegetable kingdom is a wide source of a diverse variety of enzymes with broad biotechnological applications. Among the main classes of plant enzymes, the polyphenol oxidases, which convert phenolic compounds to the related quinones, have been successfully used for biosensor development. The oxidation products from such enzymes can be electrochemically reduced, and the sensing is easily achieved by amperometric transducers. In this work, the polyphenoloxidases were extracted from jurubeba (Solanum paniculatum L.) fruits, and the extract was used to construct a carbon paste-based biosensor for pharmaceutical analysis and applications. The assay optimization was performed using a 0.1 mM catechol probe, taking into account the amount of enzymatic extract (50 or 200 \u03bcL) and the optimum pH (3.0 to 9.0) as well as some electrochemical differential pulse voltammetric (DPV) parameters (e.g., pulse amplitude, pulse range, pulse width, scan rate). Under optimized conditions, the biosensor was evaluated for the quantitative determination of acetaminophen, acetylsalicylic acid, methyldopa, and ascorbic acid. The best performance was obtained for acetaminophen, which responded linearly in the range between 5 and 245 \u03bcM (R = 0.9994), presenting a limit of detection of 3 \u03bcM and suitable repeatability ranging between 1.52% and 1.74% relative standard deviation (RSD).<\/jats:p>","DOI":"10.3390\/bios8020036","type":"journal-article","created":{"date-parts":[[2018,4,2]],"date-time":"2018-04-02T12:32:20Z","timestamp":1522672340000},"page":"36","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["The Use of a Polyphenoloxidase Biosensor Obtained from the Fruit of Jurubeba (Solanum paniculatum L.) in the Determination of Paracetamol and Other Phenolic Drugs"],"prefix":"10.3390","volume":"8","author":[{"given":"Rafael","family":"Antunes","sequence":"first","affiliation":[{"name":"Faculdade de Farm\u00e1cia, Universidade Federal do Goi\u00e1s (UFG), rua 221 esquina com a 5\u00aa avenida s\/n, Setor Universit\u00e1rio, Goi\u00e2nia-GO 74605-170, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luane","family":"Garcia","sequence":"additional","affiliation":[{"name":"Faculdade de Farm\u00e1cia, Universidade Federal do Goi\u00e1s (UFG), rua 221 esquina com a 5\u00aa avenida s\/n, Setor Universit\u00e1rio, Goi\u00e2nia-GO 74605-170, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8643-1853","authenticated-orcid":false,"given":"Vernon","family":"Somerset","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Applied Sciences, Cape Peninsula University of Technology, Bellville 7535, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Gil","sequence":"additional","affiliation":[{"name":"Faculdade de Farm\u00e1cia, Universidade Federal do Goi\u00e1s (UFG), rua 221 esquina com a 5\u00aa avenida s\/n, Setor Universit\u00e1rio, Goi\u00e2nia-GO 74605-170, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0718-3992","authenticated-orcid":false,"given":"Flavio","family":"Lopes","sequence":"additional","affiliation":[{"name":"Faculdade de Farm\u00e1cia, Universidade Federal do Goi\u00e1s (UFG), rua 221 esquina com a 5\u00aa avenida s\/n, Setor Universit\u00e1rio, Goi\u00e2nia-GO 74605-170, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,4,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"43","DOI":"10.21800\/S0009-67252012000300014","article-title":"Aprendendo com as intera\u00e7\u00f5es da natureza: Microrganismos simbiontes como fontes de produtos naturais bioativos","volume":"64","author":"Conti","year":"2012","journal-title":"Ci\u00eanc. Cult."},{"key":"ref_2","first-page":"1043","article-title":"Anti-inflammatory and antinociceptive activity of fieldgrowth plants and tissue culture of Cleome spinosa (Jacq.) in mice","volume":"7","author":"Albarello","year":"2013","journal-title":"J. Med. Plants Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1016\/S0039-9140(01)00482-9","article-title":"Chronoamperometric determination of paracetamol using an avocado tissue (Persea americana) biosensor","volume":"55","author":"Lupetti","year":"2001","journal-title":"Talanta"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/j.talanta.2016.04.046","article-title":"Talanta Recent approaches to improving selectivity and sensitivity of enzyme-based biosensors for organophosphorus pesticides: A review","volume":"155","author":"Songa","year":"2016","journal-title":"Talanta"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"850","DOI":"10.1080\/10826068.2016.1155060","article-title":"Solanummelongena polyphenol oxidase biosensor for the electrochemical analysis of paracetamol","volume":"46","author":"Garcia","year":"2016","journal-title":"Prep. Biochem. Biotechnol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"181","DOI":"10.14450\/2318-9312.v29.e3.a2017.pp181-198","article-title":"Enzimas vegetais: Extra\u00e7\u00e3o e aplica\u00e7\u00f5es biotecnol\u00f3gicas","volume":"29","author":"Antunes","year":"2017","journal-title":"Infarma"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Rodrigues, N.F.M., Neto, S.Y., Luz, R.C.S., Damos, F.S., and Yamanaka, H. (2018). Ultrasensitive Determination of Malathion Using Acetylcholinesterase Immobilized on Chitosan-Functionalized Magnetic Iron Nanoparticles. Biosensors, 8.","DOI":"10.3390\/bios8010016"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"48","DOI":"10.32404\/rean.v2i4.740","article-title":"Propriedades f\u00edsico-qu\u00edmicas de frutos de jurubeba de tr\u00eas regi\u00f5es do Cerrado","volume":"2","author":"Campos","year":"2015","journal-title":"Rev. Agric. Neotrop."},{"key":"ref_9","first-page":"124","article-title":"Biosensor-based real-time mornitoring of paracetamolphotocatalytic degradation","volume":"9","author":"Bontoux","year":"2015","journal-title":"Chemosphere"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Daher, D., Gourrierec, S.L., and P\u00e9rez-Lamela, C. (2017). Effect of high pressure processing on the microbial inactivation in fruit preparations and other vegetable based beverages. Agriculture, 7.","DOI":"10.3390\/agriculture7090072"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1016\/j.snb.2015.09.125","article-title":"A novel glucose sensor based on immobilization of glucose oxidase on the chitosan-coated Fe3O4 nanoparticles and the luminol-H2O2-gold nanoparticle chemiluminescence detection system","volume":"223","author":"Chaichi","year":"2016","journal-title":"Sens. Actuators B Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.talanta.2012.01.058","article-title":"Polymer modified glassy carbon electrode for the electrochemical determination of caffeine in coffee","volume":"93","author":"Amare","year":"2012","journal-title":"Talanta"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/j.jelechem.2017.06.032","article-title":"Electrochemical acetylcholinesterase biosensor based on multi-walled carbon nanotubes\/dicyclohexyl phthalate modified screen-printed electrode for detection of chlorpyrifos","volume":"801","author":"Chen","year":"2017","journal-title":"J. Electroanal. Chem."},{"key":"ref_14","first-page":"2999","article-title":"Utiliza\u00e7\u00e3o das enzimas peroxidases do nabo no controle do escurecimento enzim\u00e1tico em ma\u00e7\u00e3s minimamente processadas","volume":"1","author":"Hartwig","year":"2015","journal-title":"Blucher Chem. Eng. Proc."},{"key":"ref_15","first-page":"1107","article-title":"Determina\u00e7\u00e3o espectrofotom\u00e9trica de metildopa em ensaio de dissolu\u00e7\u00e3o de comprimidos utilizando extrato de rabanete como fonte de peroxidase","volume":"38","author":"Bueno","year":"2015","journal-title":"Quim. Nova"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.ab.2012.06.025","article-title":"Acetylcholinesterase inhibition-based biosensors for pesticide determination: A review","volume":"429","author":"Pundir","year":"2012","journal-title":"Anal. Biochem."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Adiguzel, Y. (2018). Biosensor with UV Spectrophotometric Detection. Proceedings, 2.","DOI":"10.3390\/ecsa-4-04928"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Peterson, R.D., Wilund, K.R., Cunningham, B.T., and Andrade, J.E. (2017). Comparison of Methods Study between a Photonic Crystal Biosensor and Certified ELISA to Measure Biomarkers of Iron Deficiency in Chronic Kidney Disease Patients. Sensors, 17.","DOI":"10.3390\/s17102203"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"3723","DOI":"10.1039\/c2nr33134a","article-title":"Surface charge of polymer coated SPIONs influences the serum protein adsorption, colloidal stability and subsequent cell interaction in vitro","volume":"5","author":"Hirsch","year":"2013","journal-title":"Nanoscale"},{"key":"ref_20","unstructured":"The United States Pharmacopeia (USP) (2009). Rockville: United States Pharmacopeial Convention, USP. [32nd ed.]."},{"key":"ref_21","unstructured":"(2017, November 15). Farmacopeia Brasileira, Available online: http:\/\/www.anvisa.gov.br\/hotsite\/cd_Farmacopeia\/pdf\/volume2.pdf."},{"key":"ref_22","unstructured":"Gil, E.S. (2010). Controle F\u00edsico-Qu\u00edmico de Qualidade de Medicamentos, Pharmabooks. [3rd ed.]."},{"key":"ref_23","unstructured":"(2018, January 21). Ag\u00eancia Nacional de Vigil\u00e2ncia Sanit\u00e1ria (ANVISA), Available online: http:\/\/portal.anvisa.gov.br\/documents\/10181\/2721567\/RDC_166_2017_COMP.pdf\/d5fb92b3-6c6b-4130-8670-4e3263763401."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.foodchem.2015.04.040","article-title":"Blueberry polyphenol oxidase: Characterization and the kinetics of thermal and high pressure activation and inactivation","volume":"188","author":"Tefere","year":"2015","journal-title":"Food Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"Um m\u00e9todo r\u00e1pido e sens\u00edvel para a quantifica\u00e7\u00e3o de micrograma quantidades de prote\u00ednas, utilizando o princ\u00edpio da prote\u00edna corantes vincula\u00e7\u00e3o","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"ref_26","first-page":"175","article-title":"Caracteriza\u00e7\u00e3o cin\u00e9tica da enzima catecolase (Polifenoloxidase) em extratos brutos da polpa e da casca de berinjela (Solanum melongena L.)","volume":"28","author":"Polesel","year":"2010","journal-title":"J. Health Sci. Inst."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1855","DOI":"10.1016\/j.foodchem.2012.03.098","article-title":"Purification and characterization of polyphenol oxidase (PPO) from eggplant","volume":"34","author":"Mishra","year":"2012","journal-title":"Food Chem."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Ganesh, H.V.S., Noroozifar, M., and Kerman, K. (2018). EpigallocatechinGallate-Modified Graphite Paste Electrode for Simultaneous Detection of Redox-Active Biomolecules. Sensors, 18.","DOI":"10.3390\/s18010023"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/j.molcatb.2011.06.016","article-title":"A nylon membrane based amperometric biosensor for polyphenol determination","volume":"72","author":"Narang","year":"2011","journal-title":"J. Mol. Catal. B: Enzym."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1590\/S0100-40422003000100009","article-title":"Determination of paracetamol in pharmaceutical products using a carbon paste biosensor modified with crude extract of zucchini (Cucurbita pepo)","volume":"26","author":"Vieira","year":"2003","journal-title":"Qu\u00edm. Nova"}],"container-title":["Biosensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6374\/8\/2\/36\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T14:59:23Z","timestamp":1760194763000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6374\/8\/2\/36"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,4,2]]},"references-count":30,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2018,6]]}},"alternative-id":["bios8020036"],"URL":"https:\/\/doi.org\/10.3390\/bios8020036","relation":{},"ISSN":["2079-6374"],"issn-type":[{"value":"2079-6374","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,4,2]]}}}