{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:34:27Z","timestamp":1787027667009,"version":"build-2736575974"},"reference-count":27,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2018,8,14]],"date-time":"2018-08-14T00:00:00Z","timestamp":1534204800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>The present study was aimed to develop \u201cfluorine doped\u201d tin oxide glass electrode with a MoSe2 nano-urchin based electrochemical biosensor for detection of Escherichia coli Shiga toxin DNA. The study comprises two conductive electrodes, and the working electrodes were drop deposited using MoSe2 nano-urchin, and DNA sequences specific to Shiga toxin Escherichia coli. Morphological characterizations were performed using Fourier transforms infrared spectrophotometer; X-ray diffraction technique and scanning electron microscopy. All measurements were done using methylene blue as an electrochemical indicator. The proposed electrochemical geno-sensor showed good linear detection range of 1 fM\u2013100 \u00b5M with a low detection limit of 1 fM where the current response increased linearly with Escherichia coli Shiga toxin dsDNA concentration with R2 = 0.99. Additionally, the real sample was spiked with the dsDNA that shows insignificant interference. The results revealed that the developed sensing platform significantly improved the sensitivity and can provide a promising platform for effective detection of biomolecules using minute samples due to its stability and sensitivity.<\/jats:p>","DOI":"10.3390\/bios8030077","type":"journal-article","created":{"date-parts":[[2018,8,14]],"date-time":"2018-08-14T10:31:16Z","timestamp":1534242676000},"page":"77","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Development of MoSe2 Nano-Urchins as a Sensing Platform for a Selective Bio-Capturing of Escherichia coli Shiga Toxin DNA"],"prefix":"10.3390","volume":"8","author":[{"given":"Jagriti","family":"Narang","sequence":"first","affiliation":[{"name":"Amity Institute of Nanotechnology, Amity University, Noida 201313, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annu","family":"Mishra","sequence":"additional","affiliation":[{"name":"Amity Institute of Nanotechnology, Amity University, Noida 201313, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"Pilloton","sequence":"additional","affiliation":[{"name":"CNR-IC, Area della Ricercadi RM1, Via Salaria km 29.3, Monterotondo, I-00015 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alekhya","family":"VV","sequence":"additional","affiliation":[{"name":"Amity Institute of Nanotechnology, Amity University, Noida 201313, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shikha","family":"Wadhwa","sequence":"additional","affiliation":[{"name":"Amity Institute of Nanotechnology, Amity University, Noida 201313, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chandra Shekhar","family":"Pundir","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, MD University, Rohtak 124001, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manika","family":"Khanuja","sequence":"additional","affiliation":[{"name":"Centre for Nanoscience and Nanotechnology, Jamia Millia Islamia University, New Delhi 110025, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,8,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2619","DOI":"10.1016\/j.bios.2010.11.020","article-title":"Development of an electrochemical DNA biosensor with a high sensitivity of fM by dendritic gold nanostructure modified electrode","volume":"26","author":"Li","year":"2011","journal-title":"Biosens. 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