{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T14:42:00Z","timestamp":1791643320463,"version":"4.3.4"},"reference-count":39,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2019,9,29]],"date-time":"2019-09-29T00:00:00Z","timestamp":1569715200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003443","name":"Ministry of Education and Science of the Russian Federation","doi-asserted-by":"publisher","award":["14.613.21.0080"],"award-info":[{"award-number":["14.613.21.0080"]}],"id":[{"id":"10.13039\/501100003443","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biosensors"],"abstract":"<jats:p>An immunochromatographic test system has been developed for the simultaneous rapid multiplex serodiagnostics of bovine brucellosis, tuberculosis, and leukemia. The test system is based on the use of a conjugate of gold nanoparticles with the chimeric protein Cysteine-A\/G and three analytical zones with immobilized pathogen antigens: Brucella abortus lipolysaccharide, recombinant proteins MPB64 and MPB83-MPB63 of Mycobacterium bovis, and recombinant protein p24 of the bovine leukemia virus. Prototypes of the test system were tested on 98 samples of sera from healthy and infected animals. The diagnostic sensitivity of the developed test system was 92% for brucellosis, 92% for tuberculosis, and 96% for leukemia. False positive test results were not observed.<\/jats:p>","DOI":"10.3390\/bios9040115","type":"journal-article","created":{"date-parts":[[2019,9,30]],"date-time":"2019-09-30T05:58:33Z","timestamp":1569823113000},"page":"115","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Triple Immunochromatographic System for Simultaneous Serodiagnosis of Bovine Brucellosis, Tuberculosis, and Leukemia"],"prefix":"10.3390","volume":"9","author":[{"given":"Lyubov V.","family":"Barshevskaya","sequence":"first","affiliation":[{"name":"A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky prospect 33, Moscow 119071, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dmitriy V.","family":"Sotnikov","sequence":"additional","affiliation":[{"name":"A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky prospect 33, Moscow 119071, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3008-2839","authenticated-orcid":false,"given":"Anatoly V.","family":"Zherdev","sequence":"additional","affiliation":[{"name":"A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky prospect 33, Moscow 119071, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bekbolat B.","family":"Khassenov","sequence":"additional","affiliation":[{"name":"Republican State Enterprise \u201cNational Center for Biotechnology\u201d under the Science Committee of Ministry of Education and Science of the Republic of Kazakhstan, Nursultan 010000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kayrat K.","family":"Baltin","sequence":"additional","affiliation":[{"name":"Republican State Enterprise \u201cNational Center for Biotechnology\u201d under the Science Committee of Ministry of Education and Science of the Republic of Kazakhstan, Nursultan 010000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saule Z.","family":"Eskendirova","sequence":"additional","affiliation":[{"name":"Republican State Enterprise \u201cNational Center for Biotechnology\u201d under the Science Committee of Ministry of Education and Science of the Republic of Kazakhstan, Nursultan 010000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kassym K.","family":"Mukanov","sequence":"additional","affiliation":[{"name":"Republican State Enterprise \u201cNational Center for Biotechnology\u201d under the Science Committee of Ministry of Education and Science of the Republic of Kazakhstan, Nursultan 010000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kanatbek K.","family":"Mukantayev","sequence":"additional","affiliation":[{"name":"Republican State Enterprise \u201cNational Center for Biotechnology\u201d under the Science Committee of Ministry of Education and Science of the Republic of Kazakhstan, Nursultan 010000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4008-4918","authenticated-orcid":false,"given":"Boris B.","family":"Dzantiev","sequence":"additional","affiliation":[{"name":"A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky prospect 33, Moscow 119071, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,9,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fvets.2018.00310","article-title":"Can we breed cattle for lower bovine TB infectivity?","volume":"5","author":"Tsairidou","year":"2018","journal-title":"Front. Vet. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"249","DOI":"10.20506\/rst.32.1.2197","article-title":"Economics of brucellosis impact and control in low-income countries","volume":"32","author":"McDermott","year":"2013","journal-title":"Rev. Sci. 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