{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T04:16:02Z","timestamp":1781151362458,"version":"3.54.1"},"reference-count":42,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,8,17]],"date-time":"2023-08-17T00:00:00Z","timestamp":1692230400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NRC \u201cKurchatov Institute\u201d, Thematic Plan 5p.3.4"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioTech"],"abstract":"<jats:p>The paper proposed a new porous material for wound healing based on chitosan and C-phycocyanin (C-PC). In this work, C-PC was extracted from the cyanobacteria Arthrospira platensis biomass and purified through ammonium sulfate precipitation. The obtained C-PC with a purity index (PI) of 3.36 \u00b1 0.24 was loaded into a chitosan sponge from aqueous solutions of various concentrations (250, 500, and 1000 mg\/L). According to the FTIR study, chitosan did not form new bonds with C-PC, but acted as a carrier. The encapsulation efficiency value exceeded 90%, and the maximum loading capacity was 172.67 \u00b1 0.47 mg\/g. The release of C-PC from the polymer matrix into the saline medium was estimated, and it was found 50% of C-PC was released in the first hour and the maximum concentration was reached in 5\u20137 h after the sponge immersion. The PI of the released C-PC was 3.79 and 4.43 depending on the concentration of the initial solution.<\/jats:p>","DOI":"10.3390\/biotech12030055","type":"journal-article","created":{"date-parts":[[2023,8,17]],"date-time":"2023-08-17T10:47:02Z","timestamp":1692269222000},"page":"55","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Chitosan Sponges for Efficient Accumulation and Controlled Release of C-Phycocyanin"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6190-8800","authenticated-orcid":false,"given":"Yana E.","family":"Sergeeva","sequence":"first","affiliation":[{"name":"Department of Biotechnology and Bioenergy, National Research Centre \u201cKurchatov Institute\u201d, 123182 Moscow, Russia"},{"name":"Department of NBIC-Technologies, Moscow Institute of Physics and Technology, National Research University, 141701 Dolgoprudny, Moscow Region, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasia A.","family":"Zakharevich","sequence":"additional","affiliation":[{"name":"Department of Nanobiomaterials and Structures, National Research Centre \u201cKurchatov Institute\u201d, 123182 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4700-1903","authenticated-orcid":false,"given":"Daniil V.","family":"Sukhinov","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Bioenergy, National Research Centre \u201cKurchatov Institute\u201d, 123182 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra I.","family":"Koshkalda","sequence":"additional","affiliation":[{"name":"Faculty of Biotechnology, Mendeleev University of Chemical Technology of Russia, 125047 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariya V.","family":"Kryukova","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Bioenergy, National Research Centre \u201cKurchatov Institute\u201d, 123182 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9015-2117","authenticated-orcid":false,"given":"Sergey N.","family":"Malakhov","sequence":"additional","affiliation":[{"name":"Department for Resource Centre, National Research Centre \u201cKurchatov Institute\u201d, 123182 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina G.","family":"Antipova","sequence":"additional","affiliation":[{"name":"Department of Nanobiomaterials and Structures, National Research Centre \u201cKurchatov Institute\u201d, 123182 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga I.","family":"Klein","sequence":"additional","affiliation":[{"name":"Department of Nanobiomaterials and Structures, National Research Centre \u201cKurchatov Institute\u201d, 123182 Moscow, Russia"},{"name":"Research Center of Biotechnology of the Russian Academy of Sciences, A.N. Bach Institute of Biochemistry, 119071 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pavel M.","family":"Gotovtsev","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Bioenergy, National Research Centre \u201cKurchatov Institute\u201d, 123182 Moscow, Russia"},{"name":"Department of NBIC-Technologies, Moscow Institute of Physics and Technology, National Research University, 141701 Dolgoprudny, Moscow Region, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timofei E.","family":"Grigoriev","sequence":"additional","affiliation":[{"name":"Department of NBIC-Technologies, Moscow Institute of Physics and Technology, National Research University, 141701 Dolgoprudny, Moscow Region, Russia"},{"name":"Department of Nanobiomaterials and Structures, National Research Centre \u201cKurchatov Institute\u201d, 123182 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"108541","DOI":"10.1016\/j.bej.2022.108541","article-title":"Large-Scale Production of Spirulina-Based Proteins and C-Phycocyanin: A Biorefinery Approach","volume":"185","author":"Thevarajah","year":"2022","journal-title":"Biochem. Eng. J."},{"key":"ref_2","first-page":"29","article-title":"Phycobilisome a Macromolecular Complex Optimized for Light Energy Transfer","volume":"768","author":"Glazer","year":"1984","journal-title":"BBA Rev. Bioenerg."},{"key":"ref_3","first-page":"784","article-title":"Structure of C-Phycocyanin from Spirulina platensis at 2.2 A Resolution: A Novel Monoclinic Crystal Form for Phycobiliproteins in Phycobilisomes","volume":"D57","author":"Wang","year":"2001","journal-title":"Acta Cryst."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.1590\/0104-6632.20180353s20170160","article-title":"C-Phycocyanin Purification: Multiple Processes for Different Applications","volume":"35","author":"Moraes","year":"2018","journal-title":"Brazilian J. Chem. 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Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1016\/j.phymed.2005.09.006","article-title":"Purification of C-Phycocyanin from Spirulina fusiformis and Its Effect on the Induction of Urokinase-Type Plasminogen Activator from Calf Pulmonary Endothelial Cells","volume":"13","author":"Madhyastha","year":"2006","journal-title":"Phytomedicine"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.actbio.2020.03.023","article-title":"\u03ba-Carrageenan-C-Phycocyanin Based Smart Injectable Hydrogels for Accelerated Wound Recovery and Real-Time Monitoring","volume":"109","author":"Dev","year":"2020","journal-title":"Acta Biomater."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Adli, S.A., Ali, F., Azmi, A.S., Anuar, H., Nasir, N.A.M., Hasham, R., and Idris, M.K.H. (2020). Development of Biodegradable Cosmetic Patch Using a Polylactic Acid\/Phycocyanin-Alginate Composite. Polymers, 12.","DOI":"10.3390\/polym12081669"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"240","DOI":"10.2174\/1871523018666190405114524","article-title":"Phycocyanin Extracted from Oscillatoria minima Shows Antimicrobial, Algicidal, and Antiradical Activities: In Silico and In Vitro Analysis","volume":"19","author":"Venugopal","year":"2019","journal-title":"Antiinflamm. Antiallergy Agents Med. Chem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"229","DOI":"10.22159\/ijap.2018v10i6.26334","article-title":"Formulation and Development of Topical Anti Acne Formulation of Spirulina Extract","volume":"10","author":"Nihal","year":"2018","journal-title":"Int. J. Appl. Pharm."},{"key":"ref_12","first-page":"4161","article-title":"Screening for Antifungal and Antiviral Activity of C-Phycocyanin from Spirulina platensis","volume":"4","author":"Murugan","year":"2011","journal-title":"J. Pharm. Res."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Suzery, M., Majid, D., Setyawan, D., and Sutanto, H. (2016, January 25\u201327). Improvement of Stability and Antioxidant Activities by Using Phycocyanin\u2014Chitosan Encapsulation Technique. Proceedings of the IOP Conference Series: Earth and Environmental Science, Bali, Indonesia.","DOI":"10.1088\/1755-1315\/55\/1\/012052"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s10856-016-5687-4","article-title":"Phycocyanin-Encapsulating Hyalurosomes as Carrier for Skin Delivery and Protection from Oxidative Stress Damage","volume":"27","author":"Castangia","year":"2016","journal-title":"J. Mater. Sci. Mater. Med."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1097","DOI":"10.32604\/jrm.2022.018142","article-title":"A Review of Various Sources of Chitin and Chitosan in Nature","volume":"10","author":"Iber","year":"2022","journal-title":"J. Renew. Mater."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1415","DOI":"10.1016\/j.ijbiomac.2017.01.112","article-title":"Can Fungi Compete with Marine Sources for Chitosan Production?","volume":"104","author":"Ghormade","year":"2017","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_17","first-page":"186","article-title":"Extraction of Chitosan, Characterisation and Its Use for Water Purification","volume":"17","author":"Niamah","year":"2018","journal-title":"J. Saudi Soc. Agric. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1007\/PL00000584","article-title":"Human Enzymatic Activities Related to the Therapeutic Administration of Chitin Derivatives","volume":"53","author":"Muzzarelli","year":"1997","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1926","DOI":"10.1016\/j.actbio.2009.03.002","article-title":"Accelerated Wound Closure of Pressure Ulcers in Aged Mice by Chitosan Scaffolds with and without BFGF","volume":"5","author":"Park","year":"2009","journal-title":"Acta Biomater."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/0144-8617(92)90051-Q","article-title":"Depolymerization of Methyl Pyrrolidinone Chitosan by Lysozyme","volume":"19","author":"Muzzarelli","year":"1992","journal-title":"Carbohydr. Polym."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/j.ijbiomac.2021.12.007","article-title":"Facile formation of injectable quaternized chitosan\/tannic acid hydrogels with antibacterial and ROS scavenging capabilities for diabetic wound healing","volume":"195","author":"Pan","year":"2022","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"141852","DOI":"10.1016\/j.cej.2023.141852","article-title":"Highly efficient bacteria-infected diabetic wound healing employing a melanin-reinforced biopolymer hydrogel","volume":"460","author":"Xiang","year":"2023","journal-title":"Chem. Eng. J."},{"key":"ref_23","first-page":"012005","article-title":"Improvement Stability of Phycocyanin from Spirulina platensis Encapsulated by Water Soluble Chitosan Nanoparticles","volume":"Volume 414","author":"Gustiningtyas","year":"2020","journal-title":"Proceedings of the IOP Conference Series: Earth and Environmental Science"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.fbp.2013.07.008","article-title":"Preparation of Phycocyanin Microcapsules and Its Properties","volume":"92","author":"Yan","year":"2014","journal-title":"Food Bioprod. Process."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"102393","DOI":"10.1016\/j.algal.2021.102393","article-title":"Increased C-Phycocyanin Extract Purity by Flocculation of Arthrospira platensis with Chitosan","volume":"58","author":"Sukhinov","year":"2021","journal-title":"Algal Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1099\/00221287-102-1-179","article-title":"Assessment of Growth Yield of a Blue-Green Alga, Spirulina platensis, in Axenic and Continuous Culture","volume":"102","author":"Aiba","year":"1977","journal-title":"J. Gen. Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.algal.2018.02.012","article-title":"Influence of Spectral Light Quality on the Pigment Concentrations and Biomass Productivity of Arthrospira platensis","volume":"31","author":"Lima","year":"2018","journal-title":"Algal Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"123700","DOI":"10.1016\/j.biortech.2020.123700","article-title":"Effect of Light Colour and Photoperiod on Biomass Growth and Phycocyanin Production by Synechococcus PCC 6715","volume":"313","author":"Pietrzyk","year":"2020","journal-title":"Bioresour. Technol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1083\/jcb.58.2.419","article-title":"Complementary Chromatic Adaptation in a Filamentous Blue-Green Alga","volume":"58","author":"Bennett","year":"1973","journal-title":"J. Cell Biol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1007\/BF00409102","article-title":"Isolation and Characterization of Phycocyanins from the Blue-Green Alga Spirulina platensis","volume":"120","author":"Boussiba","year":"1979","journal-title":"Arch. Microbiol."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Kurpan, D., Id\u00e0, A., K\u00f6rner, F., Lauceri, R., Rocculi, P., Phillips, R., and Schievano, A. (2023). Pilot-Scale Concentration and Partial Purification of Food-Grade Phycocyanin from Arthrospira platensis via Cross Flow Filtration: From Biomass to Final Product. J. Appl. Phycol.","DOI":"10.1007\/s10811-023-03014-y"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1016\/j.ijbiomac.2020.05.111","article-title":"Preparation and Characterization of C-Phycocyanin Coated with STMP\/STPP Cross-Linked Starches from Different Botanical Sources","volume":"159","author":"Lemos","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1007\/s40502-014-0094-7","article-title":"Extraction and Purification of C-Phycocyanin from Spirulina platensis (CCC540)","volume":"19","author":"Kumar","year":"2014","journal-title":"Indian J. Plant Physiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2424","DOI":"10.1016\/j.phytochem.2006.08.004","article-title":"Purification and Characterization of Selenium-Containing Phycocyanin from Selenium-Enriched Spirulina platensis","volume":"67","author":"Chen","year":"2006","journal-title":"Phytochemistry"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1016\/j.jpha.2021.12.005","article-title":"Physicochemical Degradation of Phycocyanin and Means to Improve Its Stability: A Short Review","volume":"12","author":"Adjali","year":"2022","journal-title":"J. Pharm. Anal."},{"key":"ref_36","first-page":"PA59","article-title":"Wound Healing Effects of C-Phycocyanin Isolated from Spirulina platensis","volume":"75","author":"Atilla","year":"2009","journal-title":"Planta Med."},{"key":"ref_37","first-page":"425","article-title":"In Vitro and In Vivo Investigations of the Wound Wealing Effect of Crude Spirulina Extract and C-Phycocyanin","volume":"7","author":"Gur","year":"2013","journal-title":"J. Med. Plants Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1824","DOI":"10.1080\/13880209.2017.1331249","article-title":"In Vitro Evaluation of Spirulina platensis Extract Incorporated Skin Cream With its Wound Healing and Antioxidant Activities","volume":"55","author":"Gunes","year":"2017","journal-title":"Pharm. Biol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1007\/978-3-030-75506-5_54","article-title":"Study on Extracting Crude Phycocyanin from Spirulina Algae and Determining Its Ability in Protecting Fibroblasts from Oxidative Stress of Hydroxyl Peroxide. BME 2020","volume":"85","author":"To","year":"2022","journal-title":"IFMBE Proc."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1685","DOI":"10.1007\/s10811-023-02988-z","article-title":"In Vitro Characterization and Assessment of a Potential Cosmetic Cream Containing Phycocyanin Extracted from Arthrospira platensis BUUC1503 Blue-Green Algae","volume":"35","author":"Setthamongkol","year":"2023","journal-title":"J. Appl. Phycol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"100905","DOI":"10.1016\/j.scp.2022.100905","article-title":"Chitosan\/Collagen-Based Hydrogels for Sustainable Development: Phycocyanin Controlled Release","volume":"31","author":"Azaza","year":"2023","journal-title":"Sustain. Chem. Pharm."},{"key":"ref_42","unstructured":"Suzery, M., Setyawan, D., Majid, D., and Sutanto, H. (2016, January 25\u201327). Encapsulation of Phycocyanin-Alginate for High Stability and Antioxidant Activity. 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