{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T21:17:31Z","timestamp":1777324651831,"version":"3.51.4"},"reference-count":61,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,5,25]],"date-time":"2024-05-25T00:00:00Z","timestamp":1716595200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioTech"],"abstract":"<jats:p>Candida species are frequently implicated in the development of both superficial and invasive fungal infections, which can impact vital organs. In the quest for novel strategies to combat fungal infections, there has been growing interest in exploring synthetic and semi-synthetic products, particularly chromone derivatives, renowned for their antimicrobial properties. In the analysis of the antifungal activity of the compound (E)-benzylidene-chroman-4-one against Candida, in silico and laboratory tests were performed to predict possible mechanisms of action pathways, and in vitro tests were performed to determine antifungal activity (MIC and MFC), to verify potential modes of action on the fungal cell membrane and wall, and to assess cytotoxicity in human keratinocytes. The tested compound exhibited predicted affinity for all fungal targets, with the highest predicted affinity observed for thymidylate synthase (\u2212102.589 kJ\/mol). MIC and CFM values ranged from 264.52 \u03bcM (62.5 \u03bcg\/mL) to 4232.44 \u03bcM (1000 \u03bcg\/mL). The antifungal effect likely occurs due to the action of the compound on the plasma membrane. Therefore, (E)-benzylidene-chroman-4-one showed fungicidal-like activity against Candida spp., possibly targeting the plasma membrane.<\/jats:p>","DOI":"10.3390\/biotech13020016","type":"journal-article","created":{"date-parts":[[2024,5,27]],"date-time":"2024-05-27T05:14:02Z","timestamp":1716786842000},"page":"16","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["In Silico and In Vitro Evaluation of the Antifungal Activity of a New Chromone Derivative against Candida spp."],"prefix":"10.3390","volume":"13","author":[{"given":"Gleycyelly Rodrigues","family":"Ara\u00fajo","sequence":"first","affiliation":[{"name":"Department of Clinical and Social Dentistry, Federal University of Para\u00edba, Campus I, Jo\u00e3o Pessoa 58051-900, PB, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Palloma Christine Queiroga Gomes da","family":"Costa","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Dentistry, Department of Clinic and Social Dentistry, Center of Health Sciences, Federal University of Para\u00edba, Jo\u00e3o Pessoa 58051-900, PB, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paula Lima","family":"Nogueira","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Dentistry, Department of Clinic and Social Dentistry, Center of Health Sciences, Federal University of Para\u00edba, Jo\u00e3o Pessoa 58051-900, PB, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danielle da N\u00f3brega","family":"Alves","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Dentistry, Department of Clinic and Social Dentistry, Center of Health Sciences, Federal University of Para\u00edba, Jo\u00e3o Pessoa 58051-900, PB, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alana Rodrigues","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Natural and Synthetic Bioactive Products, Federal University of Para\u00edba, Jo\u00e3o Pessoa 58051-900, PB, Brazil"},{"name":"Health Sciences Center, Federal University of Para\u00edba, Campus I, Jo\u00e3o Pessoa 58051-900, PB, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8354-3198","authenticated-orcid":false,"given":"Pablo R.","family":"da Silva","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Dentistry, Department of Clinic and Social Dentistry, Center of Health Sciences, Federal University of Para\u00edba, Jo\u00e3o Pessoa 58051-900, PB, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00e9ssica Cabral","family":"de Andrade","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Natural and Synthetic Bioactive Products, Federal University of Para\u00edba, Jo\u00e3o Pessoa 58051-900, PB, Brazil"},{"name":"Health Sciences Center, Federal University of Para\u00edba, Campus I, Jo\u00e3o Pessoa 58051-900, PB, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nat\u00e1lia F.","family":"de Sousa","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Natural and Synthetic Bioactive Products, Federal University of Para\u00edba, Jo\u00e3o Pessoa 58051-900, PB, Brazil"},{"name":"Health Sciences Center, Federal University of Para\u00edba, Campus I, Jo\u00e3o Pessoa 58051-900, PB, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-4349-0236","authenticated-orcid":false,"given":"Paulo Bruno Araujo","family":"Loureiro","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Natural and Synthetic Bioactive Products, Federal University of Para\u00edba, Jo\u00e3o Pessoa 58051-900, PB, Brazil"},{"name":"Health Sciences Center, Federal University of Para\u00edba, Campus I, Jo\u00e3o Pessoa 58051-900, PB, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7740-034X","authenticated-orcid":false,"given":"Marianna Vieira","family":"Sobral","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Natural and Synthetic Bioactive Products, Federal University of Para\u00edba, Jo\u00e3o Pessoa 58051-900, PB, Brazil"},{"name":"Health Sciences Center, Federal University of Para\u00edba, Campus I, Jo\u00e3o Pessoa 58051-900, PB, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7180-4896","authenticated-orcid":false,"given":"Dami\u00e3o P.","family":"Sousa","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Natural and Synthetic Bioactive Products, Federal University of Para\u00edba, Jo\u00e3o Pessoa 58051-900, PB, Brazil"},{"name":"Health Sciences Center, Federal University of Para\u00edba, Campus I, 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Products, Federal University of Para\u00edba, Jo\u00e3o Pessoa 58051-900, PB, Brazil"},{"name":"Health Sciences Center, Federal University of Para\u00edba, Campus I, Jo\u00e3o Pessoa 58051-900, PB, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1866-4107","authenticated-orcid":false,"given":"Luciana","family":"Scotti","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Natural and Synthetic Bioactive Products, Federal University of Para\u00edba, Jo\u00e3o Pessoa 58051-900, PB, Brazil"},{"name":"Health Sciences Center, Federal University of Para\u00edba, Campus I, Jo\u00e3o Pessoa 58051-900, PB, Brazil"},{"name":"Institute of Drugs and Medicines Research, Federal University of Para\u00edba, Via Ip\u00ea Amarelo, S\/N, Jo\u00e3o Pessoa 58051-900, PB, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/j.bjid.2013.02.001","article-title":"Brazilian Guidelines for the Management of Candidiasis\u2014A Joint Meeting Report of Three Medical Societies: Sociedade Brasileira de Infectologia, Sociedade Paulista de Infectologia and Sociedade Brasileira de Medicina Tropical","volume":"17","author":"Colombo","year":"2013","journal-title":"Brazilian J. 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