{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:15:11Z","timestamp":1760145311447,"version":"build-2065373602"},"reference-count":44,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2024,7,4]],"date-time":"2024-07-04T00:00:00Z","timestamp":1720051200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioTech"],"abstract":"<jats:p>Rutin, a flavonoid phytochemical compound, plays a vital role in human health. It is used in treating capillary fragility and has anti-Alzheimer, anti-inflammatory, and antioxidant effects. In this study, Ti-Mo-Ni-O nanotubes (NTs) were used, for the first time, in an unprecedented plant biotechnology application, wherein in vitro Philodendron shoots (Philodendron erubescens) known as \u201cImperial Red\u201d were targeted for rutin accumulation. The antioxidant responses and the accumulation of rutin were evaluated in treated Philodendron erubescens (P. erubescens) shoots using 5.0 mg\/L of Ti-Mo-Ni-O NTs. The total phenolic content and total flavonoid content were estimated, and an ABTS+ assay, FRAP assay, and iron metal chelation assay were performed. The application of Ti-Mo-Ni-O NTs enhanced the rutin content considerably from 0.02 mg\/g to 2.96 mg\/g for dry-weight shootlet extracts. Therefore, the use of Ti-Mo-Ni-O NTs is proposed to be a superior alternative to elevate the rutin content. The aim of the current study is to target P. erubescens shoots grown in vitro for the accumulation of rutin compounds using Ti-Mo-Ni-O NT powder, to determine the quantitative and qualitative accumulation of rutin via HPLC\u2013DAD analysis, and to estimate the antioxidant activity of P. erubescens shoot extract. This study presents a novel methodology for utilizing nano-biotechnology in the synthesis of plant secondary metabolites.<\/jats:p>","DOI":"10.3390\/biotech13030024","type":"journal-article","created":{"date-parts":[[2024,7,4]],"date-time":"2024-07-04T06:28:54Z","timestamp":1720074534000},"page":"24","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["In Vitro: The Extraordinary Enhancement in Rutin Accumulation and Antioxidant Activity in Philodendron \u201cImperial Red\u201d Plantlets Using Ti-Mo-Ni-O Nanotubes as a Novel Elicitor"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7223-6766","authenticated-orcid":false,"given":"Hanan S.","family":"Ebrahim","sequence":"first","affiliation":[{"name":"Department of Plant Biotechnology, Biotechnology Research Institute, National Research Centre (NRC), Cairo 12622, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0817-2045","authenticated-orcid":false,"given":"Nourhan M.","family":"Deyab","sequence":"additional","affiliation":[{"name":"Energy Materials Laboratory (EML), School of Sciences and Engineering, The American University in Cairo, New Cairo City 11835, Egypt"},{"name":"Physical Chemistry Department, National Research Centre (NRC), Cairo 12622, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Basamat S.","family":"Shaheen","sequence":"additional","affiliation":[{"name":"Energy Materials Laboratory (EML), School of Sciences and Engineering, The American University in Cairo, New Cairo City 11835, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0108-2859","authenticated-orcid":false,"given":"Ahmed M. M.","family":"Gabr","sequence":"additional","affiliation":[{"name":"Department of Plant Biotechnology, Biotechnology Research Institute, National Research Centre (NRC), Cairo 12622, Egypt"},{"name":"Academy of Scientific Research and Technology, 101 Kasr El Ainy St. Kasr El Ainy, Cairo 11516, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9458-3507","authenticated-orcid":false,"given":"Nageh K.","family":"Allam","sequence":"additional","affiliation":[{"name":"Energy Materials Laboratory (EML), School of Sciences and Engineering, The American University in Cairo, New Cairo City 11835, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3994","DOI":"10.4236\/ajps.2014.526417","article-title":"Chemical Characterization and Application of the Essential Oils from Chenopodium ambrosioides and Philodendron bipinnatifidum in the Control of Diabrotica speciosa (Coleoptera: Chrysomelidae)","volume":"5","author":"Santiago","year":"2014","journal-title":"Am. 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