{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T20:27:31Z","timestamp":1792441651464,"version":"4.5.0"},"reference-count":65,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T00:00:00Z","timestamp":1749168000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The Mahidol Wittayanusorn School Scholarship from the Mahidol Wittayanusorn School","award":["FF(KU-SRIU)1.67"],"award-info":[{"award-number":["FF(KU-SRIU)1.67"]}]},{"name":"Kasetsart University Research and Development Institute","award":["FF(KU-SRIU)1.67"],"award-info":[{"award-number":["FF(KU-SRIU)1.67"]}]},{"name":"International SciKU Branding (ISB), Faculty of Science, Kasetsart University","award":["FF(KU-SRIU)1.67"],"award-info":[{"award-number":["FF(KU-SRIU)1.67"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioTech"],"abstract":"<jats:p>Microalgae represent promising biotechnological platforms for bioactive compound production with pharmaceutical applications. This study investigated the phytochemical composition and biological activities of lipid extracts from three Chlorella species to evaluate their potential as antioxidant and antidiabetic sources. Lipid extraction using chloroform\u2013methanol (2:1) followed by GC\u2013MS analysis revealed distinct compound distributions: 29 compounds in C. ellipsoidea, 33 in C. sorokiniana, and 19 in C. vulgaris. Major bioactive compounds included 2-hexanol, 1,3,6-heptatriene, 4-(2,3-dimethyl-2-cyclopenten-1-yl)-4-methylpentanal, n-hexadecanoic acid, and octadecanoic acid. Biological activity screening encompassed antioxidant assessment through DPPH\u2022 and \u2022NO radical scavenging assays and FRAP analysis, while antidiabetic potential was evaluated using \u03b1-glucosidase and \u03b1-amylase inhibition assays. C. sorokiniana exhibited superior bioactivity with the highest antioxidant capacity (DPPH\u2022 IC50 = 329.03 \u00b1 4.30 \u00b5g\/mL; \u2022NO IC50 = 435.53 \u00b1 10.20 \u00b5g\/mL; FRAP = 94.74 \u00b1 5.72 mg TE\/g) and strongest enzyme inhibition (\u03b1-glucosidase IC50 = 752.75 \u00b1 57.95 \u00b5g\/mL; \u03b1-amylase IC50 = 3458.50 \u00b1 104.01 \u00b5g\/mL). This is the first report on C. sorokiniana strain KU.B2\u2032s biological properties and phytochemical profile. These findings establish C. sorokiniana as a valuable biotechnological platform for pharmaceutical bioactive compound development.<\/jats:p>","DOI":"10.3390\/biotech14020046","type":"journal-article","created":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T11:08:31Z","timestamp":1749208111000},"page":"46","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["In Vitro Antioxidant Potential, Antidiabetic Activities, and GC\u2013MS Analysis of Lipid Extracts of Chlorella Microalgae"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8873-2019","authenticated-orcid":false,"given":"Somruthai","family":"Kaeoboon","sequence":"first","affiliation":[{"name":"Department of Botany, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand"},{"name":"Department of Biology and Health Sciences, Mahidol Wittayanusorn School, Nakhon Pathom 73170, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4531-169X","authenticated-orcid":false,"given":"Rattanaporn","family":"Songserm","sequence":"additional","affiliation":[{"name":"Department of Botany, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6122-2019","authenticated-orcid":false,"given":"Rungcharn","family":"Suksungworn","sequence":"additional","affiliation":[{"name":"Department of Botany, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sutsawat","family":"Duangsrisai","sequence":"additional","affiliation":[{"name":"Department of Botany, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2389-9656","authenticated-orcid":false,"given":"Nuttha","family":"Sanevas","sequence":"additional","affiliation":[{"name":"Department of Botany, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"7542540","DOI":"10.1155\/2017\/7542540","article-title":"Generation and Role of Reactive Oxygen and Nitrogen Species Induced by Plasma, Lasers, Chemical Agents, and Other Systems in Dentistry","volume":"2017","author":"Jha","year":"2017","journal-title":"Oxidative Med. 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