{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:42:46Z","timestamp":1760060566404,"version":"build-2065373602"},"reference-count":36,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,9,3]],"date-time":"2025-09-03T00:00:00Z","timestamp":1756857600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Council of Education, Culture and Sports of the Regional Government of Castilla La Mancha (Spain)","award":["SBPLY\/17\/000376","SBPLY\/21\/180501\/000097","PAIUJA-EI_CTS02_2023","BIO-302","PID2021-122991NB-C21"],"award-info":[{"award-number":["SBPLY\/17\/000376","SBPLY\/21\/180501\/000097","PAIUJA-EI_CTS02_2023","BIO-302","PID2021-122991NB-C21"]}]},{"name":"University of Ja\u00e9n","award":["SBPLY\/17\/000376","SBPLY\/21\/180501\/000097","PAIUJA-EI_CTS02_2023","BIO-302","PID2021-122991NB-C21"],"award-info":[{"award-number":["SBPLY\/17\/000376","SBPLY\/21\/180501\/000097","PAIUJA-EI_CTS02_2023","BIO-302","PID2021-122991NB-C21"]}]},{"name":"Junta de Andaluc\u00eda","award":["SBPLY\/17\/000376","SBPLY\/21\/180501\/000097","PAIUJA-EI_CTS02_2023","BIO-302","PID2021-122991NB-C21"],"award-info":[{"award-number":["SBPLY\/17\/000376","SBPLY\/21\/180501\/000097","PAIUJA-EI_CTS02_2023","BIO-302","PID2021-122991NB-C21"]}]},{"name":"Ministry of Science and Innovation, the State Research Agency (AEI)","award":["SBPLY\/17\/000376","SBPLY\/21\/180501\/000097","PAIUJA-EI_CTS02_2023","BIO-302","PID2021-122991NB-C21"],"award-info":[{"award-number":["SBPLY\/17\/000376","SBPLY\/21\/180501\/000097","PAIUJA-EI_CTS02_2023","BIO-302","PID2021-122991NB-C21"]}]},{"name":"European Regional Development Fund","award":["SBPLY\/17\/000376","SBPLY\/21\/180501\/000097","PAIUJA-EI_CTS02_2023","BIO-302","PID2021-122991NB-C21"],"award-info":[{"award-number":["SBPLY\/17\/000376","SBPLY\/21\/180501\/000097","PAIUJA-EI_CTS02_2023","BIO-302","PID2021-122991NB-C21"]}]},{"name":"Council of Education, Culture, and Sports of the Regional Government of Castilla La Mancha (Spain)","award":["SBPLY\/17\/000376","SBPLY\/21\/180501\/000097","PAIUJA-EI_CTS02_2023","BIO-302","PID2021-122991NB-C21"],"award-info":[{"award-number":["SBPLY\/17\/000376","SBPLY\/21\/180501\/000097","PAIUJA-EI_CTS02_2023","BIO-302","PID2021-122991NB-C21"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioTech"],"abstract":"<jats:p>The adult lumbar spinal cord plays a critical role in locomotor control and somatosensory integration, whose transcriptional architecture under physiological conditions has been characterized in various studies with restricted numbers of individuals (up to four). Here, we present an integrative single-nucleus RNA sequencing (snRNA-seq) atlas of the healthy adult mouse lumbar spinal cord, assembled from over 86,000 nuclei from 16 samples across five public datasets. Using a harmonized computational pipeline, we identify all major spinal cell lineages and resolve 17 transcriptionally distinct neuronal subtypes. A central novelty of our approach is the systematic inclusion of non-coding RNAs (ncRNAs), including long non-coding RNAs (lncRNAs) and pseudogenes. By comparing transcriptomic analyses based on coding-only, non-coding-only, and combined gene sets, we show that ncRNAs, despite accounting to a 10% of the recorded information of each cell, contribute to cell type-specific signatures. This resource offers a high-resolution, ncRNA-inclusive reference for the adult spinal cord and provides a foundation for future studies on spinal plasticity, injury, and regeneration.<\/jats:p>","DOI":"10.3390\/biotech14030070","type":"journal-article","created":{"date-parts":[[2025,9,3]],"date-time":"2025-09-03T15:15:57Z","timestamp":1756912557000},"page":"70","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Single-Nucleus Transcriptomic Atlas of the Mouse Lumbar Spinal Cord: Functional Implications of Non-Coding RNAs"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9959-5275","authenticated-orcid":false,"given":"Pablo","family":"Ruiz-Amezcua","sequence":"first","affiliation":[{"name":"Molecular Neuroprotection Group, Instituto de Investigaci\u00f3n Sanitaria de Castilla-La Mancha, National Hospital for Paraplegics (SESCAM), 45071 Toledo, Spain"},{"name":"Systems Biomedicine Unit, Department of Experimental Biology, University of Ja\u00e9n, 23071 Ja\u00e9n, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miguel","family":"Nieto Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Molecular Neuroprotection Group, Instituto de Investigaci\u00f3n Sanitaria de Castilla-La Mancha, National Hospital for Paraplegics (SESCAM), 45071 Toledo, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Javier Garc\u00eda","family":"Flores","sequence":"additional","affiliation":[{"name":"Molecular Neuroprotection Group, Instituto de Investigaci\u00f3n Sanitaria de Castilla-La Mancha, National Hospital for Paraplegics (SESCAM), 45071 Toledo, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clara","family":"Plaza Alonso","sequence":"additional","affiliation":[{"name":"Molecular Neuroprotection Group, Instituto de Investigaci\u00f3n Sanitaria de Castilla-La Mancha, National Hospital for Paraplegics (SESCAM), 45071 Toledo, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9041-0705","authenticated-orcid":false,"given":"David","family":"Reigada","sequence":"additional","affiliation":[{"name":"Molecular Neuroprotection Group, Instituto de Investigaci\u00f3n Sanitaria de Castilla-La Mancha, National Hospital for Paraplegics (SESCAM), 45071 Toledo, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teresa","family":"Mu\u00f1oz-Galdeano","sequence":"additional","affiliation":[{"name":"Molecular Neuroprotection Group, Instituto de Investigaci\u00f3n Sanitaria de Castilla-La Mancha, National Hospital for Paraplegics (SESCAM), 45071 Toledo, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5418-8518","authenticated-orcid":false,"given":"Eva","family":"Vargas","sequence":"additional","affiliation":[{"name":"Systems Biomedicine Unit, Department of Experimental Biology, University of Ja\u00e9n, 23071 Ja\u00e9n, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7249-9205","authenticated-orcid":false,"given":"Rodrigo M.","family":"Maza","sequence":"additional","affiliation":[{"name":"Molecular Neuroprotection Group, Instituto de Investigaci\u00f3n Sanitaria de Castilla-La Mancha, National Hospital for Paraplegics (SESCAM), 45071 Toledo, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7135-2973","authenticated-orcid":false,"given":"Francisco J.","family":"Esteban","sequence":"additional","affiliation":[{"name":"Systems Biomedicine Unit, Department of Experimental Biology, University of Ja\u00e9n, 23071 Ja\u00e9n, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7244-794X","authenticated-orcid":false,"given":"Manuel","family":"Nieto-D\u00edaz","sequence":"additional","affiliation":[{"name":"Molecular Neuroprotection Group, Instituto de Investigaci\u00f3n Sanitaria de Castilla-La Mancha, National Hospital for Paraplegics (SESCAM), 45071 Toledo, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1038\/s41467-022-28698-7","article-title":"Author Correction: A harmonized atlas of mouse spinal cord cell types and their spatial organization","volume":"13","author":"Russ","year":"2022","journal-title":"Nat. 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