{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:03:04Z","timestamp":1785402184788,"version":"3.56.0"},"reference-count":54,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T00:00:00Z","timestamp":1779840000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Egypt\u2013Japan University of Science and Technology University"},{"id":[{"id":"https:\/\/ror.org\/02x66tk73","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioTech"],"abstract":"<jats:p>Curcumin can behave potently as an anti-inflammatory agent, but unfortunately, it suffers from a detrimental aqueous solubility and a limited bioavailability, which hinders its usage in clinical applications. Formulating a polymeric nanosized biocompatible system can increase the delivered curcumin fraction intra-articularly for inflammatory diseases. Poly(glyceryl adipate) nanoparticles were synthesized using a polycondensation process yielding six different polymers, which was followed by a preparation of different curcumin-loaded formulations using the nanoprecipitation method. The selected formulation exhibited a particle size of 115.8 \u00b1 10.9 nm, a mean PDI of 0.191 \u00b1 0.038, a zeta potential of \u221222.9 \u00b1 1.5 mV, and an EE of about 95%. This formulation was subjected to physicochemical characterization, including TEM, FTIR, DSC, and an in vitro release study. The stability was assessed in the presence of sodium sulfate at different concentrations, and the long-term stability was assessed through three months of storage. The cytocompatibility was evaluated by an MTT assay on normal and cancerous cell lines, revealing no significant cytotoxicity, and RT-qPCR was done in order to point out the anti-inflammation effect of the produced nanoparticles. Finally, a molecular docking study was held against Bruton\u2019s tyrosine kinase. The formulation\u2019s findings demonstrate the potential of nanoparticles, providing a basis for further in vivo studies targeting arthritis therapy.<\/jats:p>","DOI":"10.3390\/biotech15020037","type":"journal-article","created":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T12:31:56Z","timestamp":1779885116000},"page":"37","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Sustainable Curcumin-Loaded Poly(Glyceryl Adipate) Nanoparticles Attenuate Adjuvant-Induced Arthritis: A Comprehensive Study from Synthesis to Gene Expression Analysis"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-9938-6574","authenticated-orcid":false,"given":"Rania H. H.","family":"Ahmed","sequence":"first","affiliation":[{"name":"Biotechnology Program, Faculty of Basic and Applied Science, Egypt-Japan University of Science and Technology, New Borg El-Arab City 21934, Alexandria, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahmoud E.","family":"Soliman","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics and Industrial Pharmacy, Ain Shams University, Abbassia 11566, Cairo, Egypt"},{"name":"Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Egypt-Japan University of Science and Technology (E-JUST), New Borg El-Arab City 21934, Alexandria, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sherif F.","family":"Hammad","sequence":"additional","affiliation":[{"name":"Pharmaceutical Chemistry Department, Faculty of Pharmacy, Capital University (Formerly Helwan University), Cairo 11795, Cairo, Egypt"},{"name":"Medicinal Chemistry Department, Faculty of Pharmacy, Egypt-Japan University of Science and Technology (E-JUST), New Borg El-Arab City 21934, Alexandria, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hesham S. M.","family":"Soliman","sequence":"additional","affiliation":[{"name":"Pharmacognosy Department, Faculty of Pharmacy, Capital University (Formerly Helwan University), Cairo 11795, Cairo, Egypt"},{"name":"Pharmacognosy Department, Faculty of Pharmacy, Egypt-Japan University of Science and Technology (E-JUST), New Borg El-Arab City 21934, Alexandria, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed","family":"Abdel-Mawgood","sequence":"additional","affiliation":[{"name":"Biotechnology Program, Faculty of Basic and Applied Science, Egypt-Japan University of Science and Technology, New Borg El-Arab City 21934, Alexandria, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,27]]},"reference":[{"key":"ref_1","unstructured":"Merck & Co., Inc. (2023). Rheumatoid Arthritis (RA)\u2014Musculoskeletal and Connective Tissue Disorders; MSD Manuals Professional Edition, Merck & Co., Inc."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"95","DOI":"10.2165\/00063030-199809020-00002","article-title":"Intra-Articular Corticosteroids in Arthritic Disease","volume":"9","author":"Halberg","year":"1998","journal-title":"BioDrugs"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"J\u00f3zsa, L., Vasv\u00e1ri, G., Sinka, D., Nemes, D., Ujhelyi, Z., Vecserny\u00e9s, M., V\u00e1radi, J., Fenyvesi, F., Lekli, I., and Gy\u00f6ngy\u00f6si, A. (2022). Enhanced Antioxidant and Anti-Inflammatory Effects of Self-Nano and Microemulsifying Drug Delivery Systems Containing Curcumin. Molecules, 27.","DOI":"10.3390\/molecules27196652"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Sharifi-Rad, J., El Rayess, Y., Rizk, A.A., Sadaka, C., Zgheib, R., Zam, W., Sestito, S., Rapposelli, S., Neffe-Skoci\u0144ska, K., and Zieli\u0144ska, D. (2020). Turmeric and Its Major Compound Curcumin on Health: Bioactive Effects and Safety Profiles for Food, Pharmaceutical, Biotechnological and Medicinal Applications. Front. Pharmacol., 11.","DOI":"10.3389\/fphar.2020.01021"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"562","DOI":"10.3390\/nutraceuticals4040031","article-title":"Potential Applications of the Anti-Inflammatory, Antithrombotic and Antioxidant Health-Promoting Properties of Curcumin: A Critical Review","volume":"4","author":"Rapti","year":"2024","journal-title":"Nutraceuticals"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"4503","DOI":"10.2147\/DDDT.S327378","article-title":"Anti-Inflammatory Effects of Curcumin in the Inflammatory Diseases: Status, Limitations and Countermeasures","volume":"15","author":"Peng","year":"2021","journal-title":"Drug Des. Dev. Ther."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.nut.2018.03.055","article-title":"Micellar solubilisation enhances the antiinflammatory activities of curcumin and boswellic acids in rats with adjuvant-induced arthritis","volume":"54","author":"Khayyal","year":"2018","journal-title":"Nutrition"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Hafez Ghoran, S., Calcaterra, A., Abbasi, M., Taktaz, F., Nieselt, K., and Babaei, E. (2022). Curcumin-Based Nanoformulations: A Promising Adjuvant towards Cancer Treatment. Molecules, 27.","DOI":"10.3390\/molecules27165236"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1007\/s11916-025-01378-z","article-title":"The Current Status and Future Prospects of Intra-articular Injection Therapy for Hip Osteoarthritis: A Review","volume":"29","author":"Li","year":"2025","journal-title":"Curr. Pain Headache Rep."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.yexmp.2008.12.004","article-title":"Nanoparticle-based targeted drug delivery","volume":"86","author":"Singh","year":"2009","journal-title":"Exp. Mol. Pathol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"3099","DOI":"10.2147\/IJN.S210320","article-title":"Nano Encapsulated Curcumin: And Its Potential for Biomedical Applications","volume":"15","author":"Chen","year":"2020","journal-title":"Int. J. Nanomed."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Kou, H., Huang, L., Jin, M., He, Q., Zhang, R., and Ma, J. (2023). Effect of curcumin on rheumatoid arthritis: A systematic review and meta-analysis. Front. Immunol., 14.","DOI":"10.3389\/fimmu.2023.1121655"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Mohammadian Haftcheshmeh, S., Khosrojerdi, A., Aliabadi, A., Lotfi, S., Mohammadi, A., and Momtazi-Borojeni, A.A. (2020). Immunomodulatory Effects of Curcumin in Rheumatoid Arthritis: Evidence from Molecular Mechanisms to Clinical Outcomes, Springer.","DOI":"10.1007\/112_2020_54"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.colsurfa.2012.12.006","article-title":"Synthesis, characterization, biodegradability and surfactant properties of bio-sourced lauroyl poly(glycerol-succinate) oligoesters","volume":"419","author":"Agach","year":"2013","journal-title":"Colloids Surf. A Physicochem. Eng. Asp."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"130698","DOI":"10.1016\/j.ijbiomac.2024.130698","article-title":"Novel green-based polyglycerol polymeric nanoparticles loaded with ferulic acid: A promising approach for hepatoprotection","volume":"264","author":"Mahmoud","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Swainson, S.M.E., Styliari, I.D., Taresco, V., and Garnett, M.C. (2019). Poly(glycerol adipate) (PGA), an Enzymatically Synthesized Functionalizable Polyester and Versatile Drug Delivery Carrier: A Literature Update. Polymers, 11.","DOI":"10.3390\/polym11101561"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"7925","DOI":"10.1021\/acs.macromol.0c01709","article-title":"Enzymatic Synthesis of Poly(glycerol sebacate): Kinetics, Chain Growth, and Branching Behavior","volume":"53","author":"Perin","year":"2020","journal-title":"Macromolecules"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1007\/s11746-011-1910-0","article-title":"Lewis Acid-Catalyzed Synthesis of Hyperbranched Polymers Based on Glycerol and Diacids in Toluene","volume":"89","author":"Wyatt","year":"2012","journal-title":"J. Am. Oil Chem. Soc."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"10498","DOI":"10.1021\/jf4031508","article-title":"Comparative Study of Antioxidant Properties and Total Phenolic Content of the Extracts of Humulus lupulus L. and Quantification of Bioactive Components by LC\u2013MS\/MS and GC\u2013MS","volume":"61","author":"Ay","year":"2013","journal-title":"J. Agric. Food Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1007\/s12668-024-01675-8","article-title":"Development of Water-Soluble Nanoformulations of Novel Pyrazolone Derivatives and the Evaluation of Their Antibacterial and Antioxidant Activities","volume":"15","author":"Igbokwe","year":"2025","journal-title":"BioNanoScience"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/j.colsurfb.2018.08.069","article-title":"Cellulose acetate\u2014Essential oil nanocapsules with antimicrobial activity for biomedical applications","volume":"172","author":"Liakos","year":"2018","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"126928","DOI":"10.1016\/j.colsurfa.2021.126928","article-title":"Solvent impact on polymer nanoparticles prepared nanoprecipitation","volume":"625","year":"2021","journal-title":"Colloids Surf. A Physicochem. Eng. Asp."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/j.ejpb.2019.07.015","article-title":"Exploring the enzymatic degradation of poly(glycerol adipate)","volume":"142","author":"Swainson","year":"2019","journal-title":"Eur. J. Pharm. Biopharm."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Miladi, K., Sfar, S., Fessi, H., and Elaissari, A. (2016). Nanoprecipitation Process: From Particle Preparation to In Vivo Applications. Polymer Nanoparticles for Nanomedicines, Springer International Publishing.","DOI":"10.1007\/978-3-319-41421-8_2"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"10322","DOI":"10.1038\/srep10322","article-title":"Curcumin-Encapsulated Polymeric Micelles Suppress the Development of Colon Cancer In Vitro and In Vivo","volume":"5","author":"Yang","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_26","first-page":"100119","article-title":"Characterization of curcumin-loaded lecithin-chitosan bioactive nanoparticles","volume":"2","author":"Valencia","year":"2021","journal-title":"Carbohydr. Polym. Technol. Appl."},{"key":"ref_27","first-page":"115","article-title":"Development and validation of UV-Visible spectrophotometric method for the estimation of curcumin in bulk and pharmaceutical formulation","volume":"11","author":"Majumder","year":"2020","journal-title":"Pharmacophore"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"5479","DOI":"10.52711\/0974-360X.2021.00956","article-title":"Validation UV Spectrophotometric Method to Determine Entrapment Efficiency of Ocular Polymeric Nanoparticle Levofloxacin Hemihydrate","volume":"14","author":"Maharini","year":"2021","journal-title":"Res. J. Pharm. Technol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"111838","DOI":"10.1016\/j.colsurfb.2021.111838","article-title":"Systematically designed chitosan-coated solid lipid nanoparticles of ferulic acid for effective management of Alzheimer\u2019s disease: A preclinical evidence","volume":"205","author":"Saini","year":"2021","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"3267","DOI":"10.1002\/pola.28215","article-title":"Properties of acyl modified poly(glycerol-adipate) comb-like polymers and their self-assembly into nanoparticles","volume":"54","author":"Taresco","year":"2016","journal-title":"J. Polym. Sci. Part A Polym. Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2010","DOI":"10.1007\/s11095-016-1939-8","article-title":"Nanoparticles Based on Linear and Star-Shaped Poly(Ethylene Glycol)-Poly(\u03b5-Caprolactone) Copolymers for the Delivery of Antitubulin Drug","volume":"33","author":"Shalaby","year":"2016","journal-title":"Pharm. Res."},{"key":"ref_32","first-page":"207","article-title":"Synthesis and characterization of curcumin-loaded polymer blends and their cytocompatibility with BJ-1 fibroblasts","volume":"66","author":"Rozik","year":"2023","journal-title":"Egypt. J. Chem."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"El-Desoky, G.E., Wabaidur, S.M., Habila, M.A., and AlOthman, Z.A. (2021). Synergistic Effects of Curcumin and Nano-Curcumin against Toxicity, Carcinogenicity, and Oxidative Stress Induced by Tartrazine at Normal and Cancer Cell Levels. Catalysts, 11.","DOI":"10.3390\/catal11101203"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"3824","DOI":"10.1038\/s41598-021-81701-x","article-title":"PGMD\/curcumin nanoparticles for the treatment of breast cancer","volume":"11","author":"Kumari","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_35","first-page":"3739","article-title":"Lyopene Nanoparticles Ameliorate the Antioxidants, Antimicrobial and Anticancer Potencies of Tomato Pomace","volume":"64","author":"MAhmed","year":"2021","journal-title":"Egypt. J. Chem."},{"key":"ref_36","first-page":"2101","article-title":"S-limonene loaded gum Arabic nanoparticles ameliorates wound healing and inhibits herpes simplex virus","volume":"67","author":"Abdelaziz","year":"2024","journal-title":"Egypt. J. Chem."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"AbouAitah, K., Soliman, A.A.F., Swiderska-Sroda, A., Nassrallah, A., Smalc-Koziorowska, J., Gierlotka, S., and Lojkowski, W. (2022). Co-Delivery System of Curcumin and Colchicine Using Functionalized Mesoporous Silica Nanoparticles Promotes Anticancer and Apoptosis Effects. Pharmaceutics, 14.","DOI":"10.3390\/pharmaceutics14122770"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Nakiou, E.A., Lazaridou, M., Pouroutzidou, G.K., Michopoulou, A., Tsamesidis, I., Liverani, L., Arango-Ospina, M., Beketova, A., Boccaccini, A.R., and Kontonasaki, E. (2022). Poly(Glycerol Succinate) as Coating Material for 1393 Bioactive Glass Porous Scaffolds for Tissue Engineering Applications. Polymers, 14.","DOI":"10.3390\/polym14225028"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"314\u2212322","DOI":"10.1016\/j.joca.2012.01.005","article-title":"Anti-inflammatory effect of low intensity ultrasound (LIUS) on complete Freund\u2019s adjuvant-induced arthritis synovium","volume":"20","author":"Chung","year":"2012","journal-title":"Osteoarthr. Cartil."},{"key":"ref_40","first-page":"8353472","article-title":"Ameliorative Effect of Curcumin Nanoparticles against Monosodium Iodoacetate-Induced Knee Osteoarthritis in Rats","volume":"2022","author":"Farghali","year":"2022","journal-title":"Mediat. Inflamm."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1162\/1535350042973562","article-title":"Quantitative analysis of micro-CT imaging and histopathological signatures of experimental arthritis in rats","volume":"3","author":"Silva","year":"2004","journal-title":"Mol. Imaging"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.progpolymsci.2014.11.002","article-title":"From monomers to polymers from renewable resources: Recent advances","volume":"48","author":"Gandini","year":"2015","journal-title":"Prog. Polym. Sci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1007\/s10562-017-2263-6","article-title":"Clean Adipic Acid Synthesis from Liquid-Phase Oxidation of Cyclohexanone and Cyclohexanol Using (NH4)xAyPMo12O40 (A: Sb, Sn, Bi) Mixed Heteropolysalts and Hydrogen Peroxide in Free Solvent","volume":"148","author":"Mouheb","year":"2018","journal-title":"Catal. Lett."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"38673","DOI":"10.1039\/C5RA03254G","article-title":"Environmentally benign synthesis of vinyl ester resin from biowaste glycerin","volume":"5","author":"Shah","year":"2015","journal-title":"RSC Adv."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.polymer.2016.02.036","article-title":"Variation in structure and properties of poly(glycerol adipate) via control of chain branching during enzymatic synthesis","volume":"89","author":"Taresco","year":"2016","journal-title":"Polymer"},{"key":"ref_46","first-page":"1570","article-title":"Response surface methodology based optimized purification of the residual glycerol from biodiesel production process","volume":"44","author":"Danish","year":"2017","journal-title":"Chiang Mai J. Sci."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1166\/jbmb.2012.1189","article-title":"Quantification of monomers in poly(glycerol-co-diacid) gels using gas chromatography","volume":"6","author":"Wyatt","year":"2012","journal-title":"J. Biobased Mater. Bioenergy"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"137676","DOI":"10.1016\/j.foodchem.2023.137676","article-title":"Nanoparticle encapsulation using self-assembly abietic acid to improve oral bioavailability of curcumin","volume":"436","author":"Han","year":"2024","journal-title":"Food Chem."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1038\/nrrheum.2013.159","article-title":"Progress in intra-articular therapy","volume":"10","author":"Evans","year":"2014","journal-title":"Nat. Rev. Rheumatol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"191","DOI":"10.3797\/scipharm.ISP.2015.10","article-title":"Curcumin-Loaded PLA Nanoparticles: Formulation and Physical Evaluation","volume":"84","author":"Rachmawati","year":"2016","journal-title":"Sci. Pharm."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1007\/s10971-023-06276-7","article-title":"Curcumin loaded as prepared electrospun titania nanofibers for post breast cancer surgeries","volume":"109","author":"Shaban","year":"2024","journal-title":"J. Sol.-Gel Sci. Technol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1016\/j.jcis.2023.03.124","article-title":"Poly(diglycerol adipate) variants as enhanced nanocarrier replacements in drug delivery applications","volume":"641","author":"Jacob","year":"2023","journal-title":"J. Colloid Interface Sci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"2467591","DOI":"10.1080\/20565623.2025.2467591","article-title":"Polymeric nanoparticles-based targeted delivery of drugs and bioactive compounds for arthritis management","volume":"11","author":"Dixit","year":"2025","journal-title":"Future Sci. OA"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Brugnoli, B., Perna, G., Alfano, S., Piozzi, A., Galantini, L., Axioti, E., Taresco, V., Mariano, A., Scotto d\u2019Abusco, A., and Vecchio Ciprioti, S. (2024). Nanostructured Poly-l-lactide and Polyglycerol Adipate Carriers for the Encapsulation of Usnic Acid: A Promising Approach for Hepatoprotection. 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