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However, the complete morphological spectrum of reactive microglia, as revealed by three-dimensional microscopic reconstruction, has not been detailed in virus limbic encephalitis. Here, using an anatomical series of brain sections, we expanded on an earlier Piry arbovirus encephalitis study to include CA1\/CA2 and assessed the morphological response of homeostatic and reactive microglia at eight days post-infection. Hierarchical cluster and linear discriminant function analyses of multimodal morphometric features distinguished microglial morphology between infected animals and controls. For a broad representation of the spectrum of microglial morphology in each defined cluster, we chose representative cells of homeostatic and reactive microglia, using the sum of the distances of each cell in relation to all the others. Based on multivariate analysis, reactive microglia of infected animals showed more complex trees and thicker branches, covering a larger volume of tissue than in control animals. This approach offers a reliable representation of microglia dispersion in the Euclidean space, revealing the morphological kaleidoscope of surveillant and reactive microglia morphotypes. Because form precedes function in nature, our findings offer a starting point for research using integrative methods to understand microglia form and function.<\/jats:p>","DOI":"10.3390\/brainsci11081009","type":"journal-article","created":{"date-parts":[[2021,7,30]],"date-time":"2021-07-30T03:46:42Z","timestamp":1627616802000},"page":"1009","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Microglial Metamorphosis in Three Dimensions in Virus Limbic Encephalitis: An Unbiased Pictorial Representation Based on a Stereological Sampling Approach of Surveillant and Reactive Microglia"],"prefix":"10.3390","volume":"11","author":[{"given":"Leonardo S\u00e1vio","family":"da Silva Cre\u00e3o","sequence":"first","affiliation":[{"name":"N\u00facleo de Pesquisas em Oncologia, Programa de P\u00f3s-Gradua\u00e7\u00e3o em Oncologia e Ci\u00eancias M\u00e9dicas, Hospital Universit\u00e1rio Jo\u00e3o de Barros Barreto, Universidade Federal do Par\u00e1, Bel\u00e9m 66073-005, Brazil"},{"name":"Laborat\u00f3rio de Investiga\u00e7\u00f5es em Neurodegenera\u00e7\u00e3o e Infec\u00e7\u00e3o, Hospital Universit\u00e1rio Jo\u00e3o de Barros Barreto, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal do Par\u00e1, Bel\u00e9m 66073-005, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o Bento Torres","family":"Neto","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Investiga\u00e7\u00f5es em Neurodegenera\u00e7\u00e3o e Infec\u00e7\u00e3o, Hospital Universit\u00e1rio Jo\u00e3o de Barros Barreto, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal do Par\u00e1, Bel\u00e9m 66073-005, Brazil"},{"name":"Faculdade de Fisioterapia e Terapia Ocupacional, Universidade Federal do Par\u00e1, Bel\u00e9m 66075-110, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camila Mendes","family":"de Lima","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Investiga\u00e7\u00f5es em Neurodegenera\u00e7\u00e3o e Infec\u00e7\u00e3o, Hospital Universit\u00e1rio Jo\u00e3o de Barros Barreto, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal do Par\u00e1, Bel\u00e9m 66073-005, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5877-8710","authenticated-orcid":false,"given":"Renata Rodrigues","family":"dos Reis","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Investiga\u00e7\u00f5es em Neurodegenera\u00e7\u00e3o e Infec\u00e7\u00e3o, Hospital Universit\u00e1rio Jo\u00e3o de Barros Barreto, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal do Par\u00e1, Bel\u00e9m 66073-005, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7848-3618","authenticated-orcid":false,"given":"Aline Andrade","family":"de 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