{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T10:49:33Z","timestamp":1787482173067,"version":"build-2736575974"},"reference-count":36,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2021,8,28]],"date-time":"2021-08-28T00:00:00Z","timestamp":1630108800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Brain Sciences"],"abstract":"<jats:p>The olfactory bulb (OB) has an essential role in the human olfactory pathway. A change in olfactory function is associated with a change of OB volume. It has been shown to predict the prognosis of olfactory loss and its volume is a biomarker for various neurodegenerative diseases, such as Alzheimer\u2019s disease. Thus far, obtaining an OB volume for research purposes has been performed by manual segmentation alone; a very time-consuming and highly rater-biased process. As such, this process dramatically reduces the ability to produce fair and reliable comparisons between studies, as well as the processing of large datasets. Our study aims to solve this by proposing a novel methodological framework for the unbiased measurement of OB volume. In this paper, we present a fully automated tool that successfully performs such a task, accurately and quickly. In order to develop a stable and versatile algorithm and to train the neural network, we used four datasets consisting of whole-brain T1 and high-resolution T2 MRI scans, as well as the corresponding clinical information of the subject\u2019s smelling ability. One dataset contained data of patients suffering from anosmia or hyposmia (N = 79), and the other three datasets contained data of healthy controls (N = 91). First, the manual segmentation labels of the OBs were created by two experienced raters, independently and blinded. The algorithm consisted of the following four different steps: (1) multimodal data co-registration of whole-brain T1 images and T2 images, (2) template-based localization of OBs, (3) bounding box construction, and lastly, (4) segmentation of the OB using a 3D-U-Net. The results from the automated segmentation algorithm were tested on previously unseen data, achieving a mean dice coefficient (DC) of 0.77 \u00b1 0.05, which is remarkably convergent with the inter-rater DC of 0.79 \u00b1 0.08 estimated for the same cohort. Additionally, the symmetric surface distance (ASSD) was 0.43 \u00b1 0.10. Furthermore, the segmentations produced using our algorithm were manually rated by an independent blinded rater and have reached an equivalent rating score of 5.95 \u00b1 0.87 compared to a rating score of 6.23 \u00b1 0.87 for the first rater\u2019s segmentation and 5.92 \u00b1 0.81 for the second rater\u2019s manual segmentation. Taken together, these results support the success of our tool in producing automatic fast (3\u20135 min per subject) and reliable segmentations of the OB, with virtually matching accuracy with the current gold standard technique for OB segmentation. In conclusion, we present a newly developed ready-to-use tool that can perform the segmentation of OBs based on multimodal data consisting of T1 whole-brain images and T2 coronal high-resolution images. The accuracy of the segmentations predicted by the algorithm matches the manual segmentations made by two well-experienced raters. This method holds potential for immediate implementation in clinical practice. Furthermore, its ability to perform quick and accurate processing of large datasets may provide a valuable contribution to advancing our knowledge of the olfactory system, in health and disease. Specifically, our framework may integrate the use of olfactory bulb volume (OBV) measurements for the diagnosis and treatment of olfactory loss and improve the prognosis and treatment options of olfactory dysfunctions.<\/jats:p>","DOI":"10.3390\/brainsci11091141","type":"journal-article","created":{"date-parts":[[2021,8,29]],"date-time":"2021-08-29T21:45:16Z","timestamp":1630273516000},"page":"1141","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Automatic Segmentation of the Olfactory Bulb"],"prefix":"10.3390","volume":"11","author":[{"given":"Dmitriy","family":"Desser","sequence":"first","affiliation":[{"name":"Smell & Taste Clinic, Department of Otorhinolaryngology, Technische Universit\u00e4t, 01307 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisca","family":"Assun\u00e7\u00e3o","sequence":"additional","affiliation":[{"name":"Department of Informatics, School of Engineering, University of Minho, 4704-553 Braga, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoguang","family":"Yan","sequence":"additional","affiliation":[{"name":"Smell & Taste Clinic, Department of Otorhinolaryngology, Technische Universit\u00e4t, 01307 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1819-7051","authenticated-orcid":false,"given":"Victor","family":"Alves","sequence":"additional","affiliation":[{"name":"Department of Informatics, School of Engineering, University of Minho, 4704-553 Braga, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrique M.","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Center for Music in the Brain, Department of Clinical Medicine, Aarhus University, Noerrebrogade 44, 1A, 8000 Aarhus, Denmark"},{"name":"Center of Functionally Integrative Neuroscience, Aarhus University, Noerrebrogade 44, 1A, 8000 Aarhus, Denmark"},{"name":"Flavour Institute, Department of Clinical Medicine, Aarhus University, Noerrebrogade 44, 1A, 8000 Aarhus, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Hummel","sequence":"additional","affiliation":[{"name":"Smell & Taste Clinic, Department of Otorhinolaryngology, Technische Universit\u00e4t, 01307 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,8,28]]},"reference":[{"key":"ref_1","unstructured":"(2021, June 09). 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