{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T20:13:45Z","timestamp":1790108025099,"version":"4.0.1"},"reference-count":33,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2022,9,15]],"date-time":"2022-09-15T00:00:00Z","timestamp":1663200000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The Major Scientific and Technological Projects of Henan Province","award":["192102310083"],"award-info":[{"award-number":["192102310083"]}]},{"name":"The Major Scientific and Technological Projects of Henan Province","award":["202102310081"],"award-info":[{"award-number":["202102310081"]}]},{"name":"The Major Scientific and Technological Projects of Henan Province","award":["GG2019023"],"award-info":[{"award-number":["GG2019023"]}]},{"name":"The Major Scientific and Technological Projects of Henan Province","award":["XJKT201909"],"award-info":[{"award-number":["XJKT201909"]}]},{"name":"The Major Scientific and Technological Projects of Henan Province","award":["22SQT0040"],"award-info":[{"award-number":["22SQT0040"]}]},{"name":"Xinxiang City Science and Technology Research Project","award":["192102310083"],"award-info":[{"award-number":["192102310083"]}]},{"name":"Xinxiang City Science and Technology Research Project","award":["202102310081"],"award-info":[{"award-number":["202102310081"]}]},{"name":"Xinxiang City Science and Technology Research Project","award":["GG2019023"],"award-info":[{"award-number":["GG2019023"]}]},{"name":"Xinxiang City Science and Technology Research Project","award":["XJKT201909"],"award-info":[{"award-number":["XJKT201909"]}]},{"name":"Xinxiang City Science and Technology Research Project","award":["22SQT0040"],"award-info":[{"award-number":["22SQT0040"]}]},{"name":"Sanquan College of Xinxiang Medical University","award":["192102310083"],"award-info":[{"award-number":["192102310083"]}]},{"name":"Sanquan College of Xinxiang Medical University","award":["202102310081"],"award-info":[{"award-number":["202102310081"]}]},{"name":"Sanquan College of Xinxiang Medical University","award":["GG2019023"],"award-info":[{"award-number":["GG2019023"]}]},{"name":"Sanquan College of Xinxiang Medical University","award":["XJKT201909"],"award-info":[{"award-number":["XJKT201909"]}]},{"name":"Sanquan College of Xinxiang Medical University","award":["22SQT0040"],"award-info":[{"award-number":["22SQT0040"]}]},{"name":"science and technology strategic cooperation project of northern Sichuan Medical College and Nanchong City","award":["192102310083"],"award-info":[{"award-number":["192102310083"]}]},{"name":"science and technology strategic cooperation project of northern Sichuan Medical College and Nanchong City","award":["202102310081"],"award-info":[{"award-number":["202102310081"]}]},{"name":"science and technology strategic cooperation project of northern Sichuan Medical College and Nanchong City","award":["GG2019023"],"award-info":[{"award-number":["GG2019023"]}]},{"name":"science and technology strategic cooperation project of northern Sichuan Medical College and Nanchong City","award":["XJKT201909"],"award-info":[{"award-number":["XJKT201909"]}]},{"name":"science and technology strategic cooperation project of northern Sichuan Medical College and Nanchong City","award":["22SQT0040"],"award-info":[{"award-number":["22SQT0040"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Brain Sciences"],"abstract":"<jats:p>Gasdermin D-executing pyroptosis mediated by NLRP3 inflammasomes has been recognized as a key pathogenesis during stroke. Hydrogen sulfide (H2S) could protect CNS against ischemia\/reperfusion (I\/R)-induced neuroinflammation, while the underlying mechanism remains unclear. The study applied the middle cerebral artery occlusion\/reperfusion (MCAO\/R) model to investigate how the brain and the retinal injuries were alleviated in sodium hydrogen sulfide (NaHS)-treated rats. The rats were assigned to four groups and received an intraperitoneal injection of 50 \u03bcmol\/kg NaHS or NaCl 15 min after surgery. Neurological deficits were evaluated using the modified neurologic severity score. The quantification of pro-inflammatory cytokines, NLRP3, caspase-1, and GSDMD were determined by ELISA and Western blot. Cortical and retinal neurodegeneration and cell pyroptosis were determined by histopathologic examination. Results showed that NaHS rescued post-stroke neurological deficits and infarct progression, improved retina injury, and attenuated neuroinflammation in the brain cortexes and the retinae. NaHS administration inhibits inflammation by blocking the NLRP3\/caspase-1\/GSDMD pathway and further suppressing neuronal pyroptosis. This is supported by the fact that it reversed the high-level of NLRP3, caspase-1, and GSDMD following I\/R. Our findings suggest that compounds with the ability to donate H2S could constitute a novel therapeutic strategy for ischemic stroke.<\/jats:p>","DOI":"10.3390\/brainsci12091245","type":"journal-article","created":{"date-parts":[[2022,9,15]],"date-time":"2022-09-15T01:48:10Z","timestamp":1663206490000},"page":"1245","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Hydrogen Sulfide Attenuates Neuroinflammation by Inhibiting the NLRP3\/Caspase-1\/GSDMD Pathway in Retina or Brain Neuron following Rat Ischemia\/Reperfusion"],"prefix":"10.3390","volume":"12","author":[{"given":"Kun-Li","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Physiology, Sanquan College of Xinxiang Medical University, Xinxiang 453000, China"},{"name":"Department of Physiology, North Sichuan Medical College, Nanchong 637007, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Hong","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Physiology, Sanquan College of Xinxiang Medical University, Xinxiang 453000, China"},{"name":"The Third Affiliated Hospital, Xinxiang Medical University, Xinxiang 453000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya-Jie","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Physiology, Sanquan College of Xinxiang Medical University, Xinxiang 453000, China"},{"name":"Department of Physiology and Pathophysiology, Xinxiang Medical University, Xinxiang 453000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying-Juan","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Physiology, Sanquan College of Xinxiang Medical University, Xinxiang 453000, China"},{"name":"Department of Physiology and Pathophysiology, Xinxiang Medical University, Xinxiang 453000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan-Kai","family":"Ren","sequence":"additional","affiliation":[{"name":"Department of Physiology, Sanquan College of Xinxiang Medical University, Xinxiang 453000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen-Di","family":"Mai","sequence":"additional","affiliation":[{"name":"Department of Physiology, Sanquan College of Xinxiang Medical University, Xinxiang 453000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Si-Yu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Physiology, Sanquan College of Xinxiang Medical University, Xinxiang 453000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Zuo","sequence":"additional","affiliation":[{"name":"Department of Physiology, Sanquan College of Xinxiang Medical University, Xinxiang 453000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen-Zhou","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pathophysiology, Xinxiang Medical University, Xinxiang 453000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong-Liang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Physiology, Sanquan College of Xinxiang Medical University, Xinxiang 453000, China"},{"name":"Department of Physiology and Pathophysiology, Xinxiang Medical University, Xinxiang 453000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chih-Huang","family":"Yang","sequence":"additional","affiliation":[{"name":"Ineye Hospital, Chengdu University of Traditional Chinese Medicine, Chengdu 610000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/S0140-6736(15)00551-6","article-title":"Cause-specific mortality for 240 causes in China during 1990\u20132013: A systematic subnational analysis for the Global Burden of Disease Study 2013","volume":"387","author":"Zhou","year":"2016","journal-title":"Lancet"},{"key":"ref_2","first-page":"3044","article-title":"Ischemia-reperfusion Injury in the Brain: Mechanisms and Potential Therapeutic Strategies","volume":"5","author":"Li","year":"2016","journal-title":"Biochem. 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