{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T08:36:48Z","timestamp":1780043808023,"version":"3.53.1"},"reference-count":57,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,6,10]],"date-time":"2023-06-10T00:00:00Z","timestamp":1686355200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["81974184"],"award-info":[{"award-number":["81974184"]}]},{"name":"National Natural Science Foundation of China","award":["20211706"],"award-info":[{"award-number":["20211706"]}]},{"name":"Key Project of Medical Science Research of Hebei Province in 2021","award":["81974184"],"award-info":[{"award-number":["81974184"]}]},{"name":"Key Project of Medical Science Research of Hebei Province in 2021","award":["20211706"],"award-info":[{"award-number":["20211706"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Brain Sciences"],"abstract":"<jats:p>Background: Our previous research revealed that inflammation plays an important role in the pathophysiology of cerebral ischemia. The function of the NOD-like receptor protein 3 (NLRP3) inflammasome is to activate the inflammatory process. Recent findings suggest that reactive oxygen species (ROS) are essential secondary messengers that activate the NLRP3 inflammasome. Hydrogen-rich saline (HS) has attracted attention for its anti-inflammatory properties. However, the protective effect and possible mechanism of HSin brain ischemia have not been well elucidated. Methods: To test the therapeutic effect of HS, we established a mouse model of distal middle cerebral artery occlusion (dMCAO) and an in vitro model of BV2 cells induced by lipopolysaccharide (LPS). The ROS scavenger N-acetylcysteine (NAC) was used to investigate the underlying mechanisms of HS. Results: HS significantly improved neurological function, reduced infarct volume, and increased cerebral blood flow in a dMCAO mouse model. ROS, NLRP3, Caspase-1, and IL-1\u03b2 expression increased after cerebral ischemia, and this was reversed by HS treatment. In BV2 cells, the application of NAC further demonstrated that HS could effectively inhibit the expression of the ROS-activated NLRP3 inflammasome. Conclusions: HS, as a novel therapeutic option, could exert protect the brain by inhibiting the activation of the ROS-NLRP3 signaling pathway after cerebral ischemia.<\/jats:p>","DOI":"10.3390\/brainsci13060939","type":"journal-article","created":{"date-parts":[[2023,6,12]],"date-time":"2023-06-12T03:02:27Z","timestamp":1686538947000},"page":"939","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Hydrogen-Rich Saline\u2014A Novel Neuroprotective Agent in a Mouse Model of Experimental Cerebral Ischemia via the ROS-NLRP3 Inflammasome Signaling Pathway In Vivo and In Vitro"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8952-2814","authenticated-orcid":false,"given":"Yuanyuan","family":"Du","sequence":"first","affiliation":[{"name":"Department of Neurology, Second Hospital of Hebei Medical University, Shijiazhuang 050000, China"},{"name":"Hebei Collaborative Innovation Center 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Neurology, Second Hospital of Hebei Medical University, Shijiazhuang 050000, China"},{"name":"Hebei Collaborative Innovation Center for Cardio-Cerebrovascular Disease, Shijiazhuang 050000, China"},{"name":"Hebei Key Laboratory of Vascular Homeostasis, Shijiazhuang 050000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lan","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Neurology, Second Hospital of Hebei Medical University, Shijiazhuang 050000, China"},{"name":"Hebei Collaborative Innovation Center for Cardio-Cerebrovascular Disease, Shijiazhuang 050000, China"},{"name":"Hebei Key Laboratory of Vascular Homeostasis, Shijiazhuang 050000, 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Neurology, Second Hospital of Hebei Medical University, Shijiazhuang 050000, China"},{"name":"Hebei Collaborative Innovation Center for Cardio-Cerebrovascular Disease, Shijiazhuang 050000, China"},{"name":"Hebei Key Laboratory of Vascular Homeostasis, Shijiazhuang 050000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1210","DOI":"10.1177\/0271678X20958587","article-title":"Long-term outcome of endovascular therapy for acute basilar artery occlusion","volume":"41","author":"Wu","year":"2021","journal-title":"J. Cereb. Blood Flow Metab."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1097\/WCO.0b013e32813ba151","article-title":"Inflammation and ischaemic stroke","volume":"20","author":"Muir","year":"2007","journal-title":"Curr. Opin. Neurol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1016\/j.jns.2018.11.004","article-title":"Hydrogen as a complementary therapy against ischemic stroke: A review of the evidence","volume":"396","author":"Li","year":"2019","journal-title":"J. Neurol. Sci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"687","DOI":"10.2174\/1381612826666201113095720","article-title":"Hydrogen-induced Neuroprotection in Neonatal Hypoxic-ischemic Encephalopathy","volume":"27","author":"Domoki","year":"2021","journal-title":"Curr. Pharm. Des."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1038\/nm1577","article-title":"Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals","volume":"13","author":"Ohsawa","year":"2007","journal-title":"Nat. Med."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/j.jhep.2010.08.011","article-title":"The protective role of hydrogen-rich saline in experimental liver injury in mice","volume":"54","author":"Sun","year":"2011","journal-title":"J. Hepatol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1016\/j.bbrc.2009.02.105","article-title":"Hydrogen-rich saline reduces lung injury induced by intestinal ischemia\/reperfusion in rats","volume":"381","author":"Mao","year":"2009","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"e95","DOI":"10.1016\/j.jss.2011.01.057","article-title":"The effect of hydrogen-rich saline on the brain of rats with transient ischemia","volume":"168","author":"Ji","year":"2011","journal-title":"J. Surg. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"63","DOI":"10.3389\/fncel.2017.00063","article-title":"NLRP3 Inflammasome in Neurological Diseases, from Functions to Therapies","volume":"11","author":"Song","year":"2017","journal-title":"Front. Cell. Neurosci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/S1097-2765(02)00599-3","article-title":"The inflammasome: A molecular platform triggering activation of inflammatory caspases and processing of proIL-beta","volume":"10","author":"Martinon","year":"2002","journal-title":"Mol. Cell"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2999","DOI":"10.1007\/s00018-012-0962-0","article-title":"Mitochondrial dysfunction and oxidative stress activate inflammasomes: Impact on the aging process and age-related diseases","volume":"69","author":"Salminen","year":"2012","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"613","DOI":"10.4049\/jimmunol.1100613","article-title":"Cutting edge: Reactive oxygen species inhibitors block priming, but not activation, of the NLRP3 inflammasome","volume":"187","author":"Bauernfeind","year":"2011","journal-title":"J. Immunol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1111\/j.1600-065X.2011.01046.x","article-title":"The inflammasome: An integrated view","volume":"243","author":"Gross","year":"2011","journal-title":"Immunol. Rev."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"674","DOI":"10.1126\/science.1156995","article-title":"Innate immune activation through Nalp3 inflammasome sensing of asbestos and silica","volume":"320","author":"Catherine","year":"2008","journal-title":"Science"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1038\/nri2725","article-title":"NLRP3 inflammasome activation: The convergence of multiple signalling pathways on ROS production","volume":"10","author":"Tschopp","year":"2010","journal-title":"Nat. Rev. Immunol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1007\/s00401-018-01957-y","article-title":"Aggregated Tau activates NLRP3-ASC inflammasome exacerbating exogenously seeded and non-exogenously seeded Tau pathology in vivo","volume":"137","author":"Stancu","year":"2019","journal-title":"Acta Neuropathol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1048","DOI":"10.1177\/0271678X19856226","article-title":"Extracellular ASC exacerbated the recurrent ischemic stroke in an NLRP3-dependent manner","volume":"40","author":"He","year":"2019","journal-title":"J. Cereb. Blood Flow Metab."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"120568","DOI":"10.1016\/j.lfs.2022.120568","article-title":"Cerebroprotein hydrolysate injection is involved in promoting long-term angiogenesis, vessel diameter and density after cerebral ischemia in mice","volume":"300","author":"Myagmar","year":"2022","journal-title":"Life Sci."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Cui, L., Murikinati, S.R., Wang, D., Zhang, X., Duan, W.M., and Zhao, L.R. (2013). Reestablishing neuronal networks in the aged brain by stem cell factor and granulocyte-colony stimulating factor in a mouse model of chronic stroke. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0064684"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"974","DOI":"10.21037\/atm-21-2489","article-title":"Hydrogen-rich saline regulates the polarization and apoptosis of alveolar macrophages and attenuates lung injury via suppression of autophagy in septic rats","volume":"9","author":"Qiu","year":"2021","journal-title":"Ann. Transl. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"106517","DOI":"10.1016\/j.intimp.2020.106517","article-title":"Hydrogen attenuates radiation-induced intestinal damage by reducing oxidative stress and inflammatory response","volume":"84","author":"Qiu","year":"2020","journal-title":"Int. Immunopharmacol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1038\/nature15257","article-title":"Labelling and optical erasure of synaptic memory traces in the motor cortex","volume":"525","author":"Yagishita","year":"2015","journal-title":"Nature"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1006","DOI":"10.1111\/cns.13146","article-title":"Protective effects of leonurine against ischemic stroke in mice by activating nuclear factor erythroid 2-related factor 2 pathway","volume":"25","author":"Xie","year":"2019","journal-title":"CNS Neurosci. Ther."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1904","DOI":"10.1007\/s11064-022-03574-4","article-title":"Liraglutide Ameliorates Cerebral Ischemia in Mice via Antipyroptotic Pathways","volume":"47","author":"Yang","year":"2022","journal-title":"Neurochem. Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"109220","DOI":"10.1016\/j.intimp.2022.109220","article-title":"Chrysophanol facilitates long-term neurological recovery through limiting microglia-mediated neuroinflammationafter ischemic stroke in mice","volume":"112","author":"Liu","year":"2022","journal-title":"Int. Immunopharmacol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"370530","DOI":"10.1155\/2014\/370530","article-title":"Chrysophanol inhibits NALP3 inflammasome activation and ameliorates cerebral ischemia\/reperfusion in mice","volume":"2014","author":"Zhang","year":"2014","journal-title":"Mediat. Inflamm."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"122549","DOI":"10.1016\/j.jhazmat.2020.122549","article-title":"Mechanism investigation on Bisphenol S-induced oxidative stress and inflammation in murine RAW264.7 cells: The role of NLRP3 inflammasome, TLR4, Nrf2 and MAPK","volume":"394","author":"Xie","year":"2020","journal-title":"J. Hazard. Mater."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"172418","DOI":"10.1016\/j.ejphar.2019.172418","article-title":"Ginsenoside Rg1 promotes cerebral angiogenesis via the PI3K\/Akt\/mTOR signaling pathway in ischemic mice","volume":"856","author":"Chen","year":"2019","journal-title":"Eur. J. Pharmacol."},{"key":"ref_29","first-page":"13043","article-title":"Effect of astaxanthin on neuron damage, inflammatory factors expressions and oxidative stress in mice with subarachnoid hemorrhage","volume":"13","author":"Qian","year":"2021","journal-title":"Am. J. Transl. Res."},{"key":"ref_30","first-page":"3448","article-title":"MCMV triggers ROS\/NLRP3-associated inflammasome activation in the inner ear of mice and cultured spiral ganglion neurons, contributing to sensorineural hearing loss","volume":"41","author":"Zhuang","year":"2018","journal-title":"Int. J. Mol. Med."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Liu, S., Tao, J., Duan, F., Li, H., and Tan, H. (2022). HHcy Induces Pyroptosis and Atherosclerosis via the Lipid Raft-Mediated NOX-ROS-NLRP3 Inflammasome Pathway in apoE-\/- Mice. Cells, 11.","DOI":"10.3390\/cells11152438"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"80","DOI":"10.4103\/2045-9912.260649","article-title":"Recent advances in the neuroprotective effects of medical gases","volume":"9","author":"Wang","year":"2019","journal-title":"Med. Gas Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"8384742","DOI":"10.1155\/2020\/8384742","article-title":"Hydrogen: A Novel Option in Human Disease Treatment","volume":"2020","author":"Yang","year":"2020","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/S1474-4422(13)70255-2","article-title":"Secondary stroke prevention","volume":"13","author":"Hankey","year":"2014","journal-title":"Lancet Neurol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1152\/physrev.00011.2010","article-title":"Physiology of microglia","volume":"91","author":"Kettenmann","year":"2011","journal-title":"Physiol. Rev."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"28","DOI":"10.3389\/fnmol.2020.00028","article-title":"Targeting Oxidative Stress and Inflammation to Prevent Ischemia-Reperfusion Injury","volume":"13","author":"Wu","year":"2020","journal-title":"Front. Mol. Neurosci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"5528","DOI":"10.1523\/JNEUROSCI.21-15-05528.2001","article-title":"Role of IL-1\u03b1 and IL-1\u03b2 in ischemic brain damage","volume":"21","author":"Boutin","year":"2001","journal-title":"J. Neurosci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1311","DOI":"10.1080\/10715762.2018.1460749","article-title":"Hydrogen-rich water improves cognitive impairment gender-dependently in APP\/PS1 mice without affecting A\u03b2 clearance","volume":"52","author":"Hou","year":"2018","journal-title":"Free Radic. Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1097\/SHK.0b013e31824ed57c","article-title":"Effect of hydrogen gas on the survival rate of mice following global cerebral ischemia","volume":"37","author":"Nagatani","year":"2012","journal-title":"Shock"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Takeuchi, S., Nagatani, K., Otani, N., Nawashiro, H., Sugawara, T., Wada, K., and Mori, K. (2015). Hydrogen improves neurological function through attenuation of blood-brain barrier disruption in spontaneously hypertensive stroke-prone rats. BMC Neurosci., 16.","DOI":"10.1186\/s12868-015-0165-3"},{"key":"ref_41","first-page":"677","article-title":"Molecular hydrogen affords neuroprotection in a translational piglet model of hypoxic-ischemic encephalopathy","volume":"67","author":"Nemeth","year":"2016","journal-title":"J. Physiol. Pharmacol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1634","DOI":"10.1159\/000484024","article-title":"Hydrogen-Rich Saline Attenuates Brain Injury Induced by Cardiopulmonary Bypass and Inhibits Microvascular Endothelial Cell Apoptosis Via the PI3K\/Akt\/GSK3\u03b2 Signaling Pathway in Rats","volume":"43","author":"Chen","year":"2017","journal-title":"Cell. Physiol. Biochem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1038\/cmi.2010.81","article-title":"The inflammasomes in health and disease: From genetics to molecular mechanisms of autoinflammation and beyond","volume":"8","author":"Mortellaro","year":"2011","journal-title":"Cell. Mol. Immunol."},{"key":"ref_44","first-page":"859145","article-title":"NLRP3 Inflammasome and MS\/EAE","volume":"2013","author":"Inoue","year":"2013","journal-title":"Autoimmune Dis."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1002\/ana.24070","article-title":"NLRP3 inflammasome contributes to inflammation after intracerebral hemorrhage","volume":"75","author":"Ma","year":"2014","journal-title":"Ann. Neurol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2072","DOI":"10.1007\/s11064-013-1115-z","article-title":"Expression of the NLRP3 inflammasome in cerebral cortexafter traumatic brain injury in a rat model","volume":"38","author":"Liu","year":"2013","journal-title":"Neurochem. Res."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1007\/s12035-013-8475-x","article-title":"The NLRP3 inflammasome in Alzheimer\u2019s disease","volume":"48","author":"Tan","year":"2013","journal-title":"Mol. Neurobiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"5440","DOI":"10.4049\/jimmunol.1100790","article-title":"The NLRP3 inflammasome contributes to brain injury in pneumococcal meningitis and is activated through ATP-dependent lysosomal cathepsin B release","volume":"187","author":"Hoegen","year":"2011","journal-title":"J. Immunol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"848","DOI":"10.1089\/ars.2014.5868","article-title":"Reactive oxygen species-induced TXNIP drives fructose-mediated hepatic inflammation and lipid accumulation through NLRP3 inflammasome activation","volume":"22","author":"Zhang","year":"2015","journal-title":"Antioxid. Redox Signal."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1097\/00004647-200208000-00008","article-title":"Elevated intracranial IL-18 in humans and mice after traumatic brain injury and evidence of neuroprotective effects of IL-18-binding protein after experimental closed head injury","volume":"22","author":"Yatsiv","year":"2002","journal-title":"J. Cereb. Blood Flow Metab."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"ftu024","DOI":"10.1093\/femspd\/ftu024","article-title":"Helicobacter pylori induces IL-1\u03b2 and IL-18 production in human monocytic cell line through activation of NLRP3 inflammasome via ROS signaling pathway","volume":"73","author":"Li","year":"2015","journal-title":"Pathog. Dis."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.exer.2014.05.001","article-title":"Reactive oxygen species activated NLRP3 inflammasomes prime environment-induced murine dry eye","volume":"125","author":"Zheng","year":"2014","journal-title":"Exp. Eye Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"101583","DOI":"10.1016\/j.mcp.2020.101583","article-title":"Insulin-like growth factor-I activates NF\u03baB and NLRP3 inflammatory signalling via ROS in cancer cells","volume":"52","author":"Wang","year":"2020","journal-title":"Mol. Cell. Probes."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"3462","DOI":"10.1007\/s12035-015-9242-y","article-title":"Hydrogen-Rich Saline Attenuated Subarachnoid Hemorrhage-Induced Early Brain Injury in Rats by Suppressing Inflammatory Response: Possible Involvement of NF-\u03baB Pathway and NLRP3 Inflammasome","volume":"53","author":"Shao","year":"2016","journal-title":"Mol. Neurobiol."},{"key":"ref_55","first-page":"331","article-title":"The Clinical Application of Hydrogen as a Medical Treatment","volume":"70","author":"Iida","year":"2016","journal-title":"Acta Med. Okayama"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"140","DOI":"10.3164\/jcbn.09-100","article-title":"Effectiveness of hydrogen rich water on antioxidant status of subjects with potential metabolic syndrome-an open label pilot study","volume":"46","author":"Nakao","year":"2010","journal-title":"J. Clin. Biochem. Nutr."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/2045-9912-1-11","article-title":"Effects of drinking hydrogen-rich water on the quality of life of patients treated with radiotherapy for liver tumors","volume":"1","author":"Kang","year":"2011","journal-title":"Med. Gas Res."}],"container-title":["Brain Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3425\/13\/6\/939\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:52:27Z","timestamp":1760125947000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3425\/13\/6\/939"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,10]]},"references-count":57,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2023,6]]}},"alternative-id":["brainsci13060939"],"URL":"https:\/\/doi.org\/10.3390\/brainsci13060939","relation":{},"ISSN":["2076-3425"],"issn-type":[{"value":"2076-3425","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,10]]}}}