{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T05:12:24Z","timestamp":1790745144437,"version":"4.1.0"},"reference-count":68,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T00:00:00Z","timestamp":1696550400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Deputyship for Research &amp; Innovation, Ministry of Education in Saudi Arabia","award":["IFKSURG-759"],"award-info":[{"award-number":["IFKSURG-759"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Brain Sciences"],"abstract":"<jats:p>A substantial percentage of pregnant smokers stop using traditional cigarettes and switch to alternative nicotine-related products such as e-cigarettes. Prenatal exposure to tobacco increases the risk of psychiatric disorders in children. Adolescence is a complex phase in which higher cognitive and emotional processes undergo maturation and refinement. In this study, we examined the behavioral and molecular effects of first-trimester prenatal exposure to e-cigarettes. Adult female mice were divided into normal air, vehicle, and 2.5%-nicotine-exposed groups. Our analyses indicated that the adolescents in the 2.5%-nicotine-exposed group exhibited a significant lack of normal digging behavior, elevated initial sucrose intake, and reduced recognition memory. Importantly, we identified a substantial level of nicotine self-administration in the 2.5%-nicotine-exposed group. At a molecular level, the mRNAs of metabotropic glutamate receptors and transporters in the nucleus accumbens were not altered. This previously undescribed work indicates that prenatal exposure to e-cigarettes might increase the risk of nicotine addiction during adolescence, reduce cognitive capacity, and alter normal adolescent behavior. The outcome will aid in translating research and assist healthcare practitioners in tackling addiction and mental issues caused by toxicological exposure. Further, it will inform relevant policymaking, such as recommended taxation, labeling e-cigarette devices with more detailed neurotoxic effects, and preventing their sale to pregnant women and adolescents.<\/jats:p>","DOI":"10.3390\/brainsci13101417","type":"journal-article","created":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T07:49:29Z","timestamp":1696578569000},"page":"1417","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Investigating Behavioral and Neuronal Changes in Adolescent Mice Following Prenatal Exposure to Electronic Cigarette (E-Cigarette) Vapor Containing Nicotine"],"prefix":"10.3390","volume":"13","author":[{"given":"Alaa","family":"AlHarthi","sequence":"first","affiliation":[{"name":"Pharmacology & Toxicology Graduate Program, Pharmacy College, King Saud University, Riyadh 11211, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2382-5892","authenticated-orcid":false,"given":"Fawaz","family":"Alasmari","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh 11362, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4263-3610","authenticated-orcid":false,"given":"Shakir D.","family":"AlSharari","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh 11362, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5283-1576","authenticated-orcid":false,"given":"Nouf M.","family":"Alrasheed","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh 11362, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5165-1095","authenticated-orcid":false,"given":"Musaad A.","family":"Alshammari","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh 11362, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2211-1014","authenticated-orcid":false,"given":"Tahani K.","family":"Alshammari","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh 11362, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,6]]},"reference":[{"key":"ref_1","first-page":"1","article-title":"Electronic Cigarette Use Among Adults: United States, 2014","volume":"217","author":"Schoenborn","year":"2015","journal-title":"NCHS Data Brief"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1136\/tobaccocontrol-2013-051043","article-title":"News media representations of electronic cigarettes: An analysis of newspaper coverage in the UK and Scotland","volume":"23","author":"Rooke","year":"2014","journal-title":"Tob. Control"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1093\/her\/cyw059","article-title":"Perceptions and use of electronic cigarettes in pregnancy","volume":"32","author":"McCubbin","year":"2017","journal-title":"Health Educ. Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1146\/annurev.pharmtox.47.120505.105214","article-title":"Nicotinic Acetylcholine Receptors and Nicotinic Cholinergic Mechanisms of the Central Nervous System","volume":"47","author":"Dani","year":"2007","journal-title":"Annu. Rev. Pharmacol. Toxicol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.pharmthera.2009.02.003","article-title":"The dynamic effects of nicotine on the developing brain","volume":"122","author":"Dwyer","year":"2009","journal-title":"Pharmacol. Ther."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1007\/s00424-017-2073-0","article-title":"Human nicotinic receptors in chromaffin cells: Characterization and pharmacology","volume":"470","author":"Albillos","year":"2018","journal-title":"Pfl\u00fcgers Arch. Eur. J. Physiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3831\/KPI.2020.23.001","article-title":"Nicotine Addiction: Neurobiology and Mechanism","volume":"23","author":"Tiwari","year":"2020","journal-title":"J. Pharmacopunct."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1016\/S2215-0366(16)00104-8","article-title":"Neurobiology of addiction: A neurocircuitry analysis","volume":"3","author":"Koob","year":"2016","journal-title":"Lancet Psychiatry"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.ejphar.2010.01.030","article-title":"Group II metabotropic glutamate receptors (mGlu2\/3) in drug addiction","volume":"639","author":"Moussawi","year":"2010","journal-title":"Eur. J. Pharmacol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1111\/adb.12443","article-title":"mGluR2\/3 mediates short-term control of nicotine-seeking by acute systemic N-acetylcysteine","volume":"23","author":"Moro","year":"2018","journal-title":"Addict. Biol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.coph.2020.07.009","article-title":"Astrocytes as cellular mediators of cue reactivity in addiction","volume":"56","author":"Kruyer","year":"2021","journal-title":"Curr. Opin. Pharmacol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.pnpbp.2017.03.017","article-title":"Effects of chronic inhalation of electronic cigarettes containing nicotine on glial glutamate transporters and \u03b1-7 nicotinic acetylcholine receptor in female CD-1 mice","volume":"77","author":"Alasmari","year":"2017","journal-title":"Prog. Neuropsychopharmacol. Biol. Psychiatry"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"885","DOI":"10.3389\/fphar.2019.00885","article-title":"Effects of Chronic Inhalation of Electronic Cigarette Vapor Containing Nicotine on Neurotransmitters in the Frontal Cortex and Striatum of C57BL\/6 Mice","volume":"10","author":"Alasmari","year":"2019","journal-title":"Front. Pharmacol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1016\/j.biopsych.2008.10.040","article-title":"The role of cystine-glutamate exchange in nicotine dependence in rats and humans","volume":"65","author":"Knackstedt","year":"2009","journal-title":"Biol. Psychiatry"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Smith, D., Aherrera, A., Lopez, A., Neptune, E., Winickoff, J.P., Klein, J.D., Chen, G., Lazarus, P., Collaco, J.M., and McGrath-Morrow, S.A. (2015). Adult Behavior in Male Mice Exposed to E-Cigarette Nicotine Vapors during Late Prenatal and Early Postnatal Life. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0137953"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"112911","DOI":"10.1016\/j.physbeh.2020.112911","article-title":"Effect of electronic cigarette aerosol exposure during gestation and lactation on learning and memory of adult male offspring rats","volume":"221","author":"Alzoubi","year":"2020","journal-title":"Physiol. Behav."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.biopsych.2009.06.022","article-title":"Environmental enrichment produces a behavioral phenotype mediated by low cyclic adenosine monophosphate response element binding (CREB) activity in the nucleus accumbens","volume":"67","author":"Green","year":"2010","journal-title":"Biol. Psychiatry"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.physbeh.2015.11.002","article-title":"The effects of nicotine self-administration and withdrawal on concurrently available chow and sucrose intake in adult male rats","volume":"154","author":"Bunney","year":"2016","journal-title":"Physiol. Behav."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.pbb.2017.08.013","article-title":"Assessment of nicotine withdrawal-induced changes in sucrose preference in mice","volume":"161","author":"Alkhlaif","year":"2017","journal-title":"Pharmacol. Biochem. Behav."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.jneumeth.2014.02.001","article-title":"Burrowing and nest building behavior as indicators of well-being in mice","volume":"234","author":"Jirkof","year":"2014","journal-title":"J. Neurosci. Methods"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.bbr.2012.10.058","article-title":"Evaluation of emotional behaviors in young offspring of C57BL\/6J mice after gestational and\/or perinatal exposure to nicotine in six different time-windows","volume":"239","author":"Alkam","year":"2013","journal-title":"Behav. Brain Res."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Carboni, L., Ponzoni, L., Braida, D., Sala, M., Gotti, C., and Zoli, M. (2021). Altered mRNA Levels of Stress-Related Peptides in Mouse Hippocampus and Caudate-Putamen in Withdrawal after Long-Term Intermittent Exposure to Tobacco Smoke or Electronic Cigarette Vapour. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22020599"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1007\/s10339-011-0430-z","article-title":"The novel object recognition memory: Neurobiology, test procedure, and its modifications","volume":"13","author":"Antunes","year":"2012","journal-title":"Cogn. Process."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1101\/lm.2274911","article-title":"Differing time dependencies of object recognition memory impairments produced by nicotinic and muscarinic cholinergic antagonism in perirhinal cortex","volume":"18","author":"Tinsley","year":"2011","journal-title":"Learn. Mem."},{"key":"ref_25","first-page":"224","article-title":"Oral Nicotine Self-Administration in Rodents","volume":"S2","author":"Collins","year":"2012","journal-title":"J. Addict. Res. Ther."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"112191","DOI":"10.1016\/j.bbr.2019.112191","article-title":"Analysis of the molecular and behavioral effects of acute social isolation on rats","volume":"377","author":"Alshammari","year":"2020","journal-title":"Behav. Brain Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1775","DOI":"10.1016\/j.euroneuro.2015.06.010","article-title":"Different physiological and behavioural effects of e-cigarette vapour and cigarette smoke in mice","volume":"25","author":"Ponzoni","year":"2015","journal-title":"Eur. Neuropsychopharmacol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1016\/j.chemosphere.2016.08.144","article-title":"The C57BL\/6J mice offspring originated from a parental generation exposed to tannery effluents shows object recognition deficits","volume":"164","author":"Ferreira","year":"2016","journal-title":"Chemosphere"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1007\/BF02247438","article-title":"Inbred mouse strains vary in oral self-selection of nicotine","volume":"124","author":"Robinson","year":"1996","journal-title":"Psychopharmacology"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1007\/s00213-006-0306-6","article-title":"Nicotine self-administration in mice is associated with rates of nicotine inactivation by CYP2A5","volume":"184","author":"Siu","year":"2006","journal-title":"Psychopharmacology"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1037\/0735-7044.119.1.26","article-title":"Modulation of nicotine but not ethanol preference by the mouse Chrna4 A529T polymorphism","volume":"119","author":"Butt","year":"2005","journal-title":"Behav. Neurosci."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Alshammari, T.K., Alghamdi, H.M., Alduhailan, H.E., Saja, M.F., Alrasheed, N.M., and Alshammari, M.A. (2020). Examining the central effects of chronic stressful social isolation on rats. Biomed. Rep., 13.","DOI":"10.3892\/br.2020.1363"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"206120","DOI":"10.5402\/2013\/206120","article-title":"Prenatal Exposure to Maternal Cigarette Smoke and Offspring Risk of Excess Weight Is Independent of Both Birth Weight and Catch-Up Growth","volume":"2013","author":"Gravel","year":"2013","journal-title":"ISRN Epidemiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1038\/oby.2005.77","article-title":"Prenatal exposure to nicotine causes postnatal obesity and altered perivascular adipose tissue function","volume":"13","author":"Gao","year":"2005","journal-title":"Obes. Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1093\/toxsci\/kfy083","article-title":"Maternal Nicotine Exposure Leads to Augmented Expression of the Antioxidant Adipose Tissue Triglyceride Lipase Long-Term in the White Adipose of Female Rat Offspring","volume":"164","author":"Barra","year":"2018","journal-title":"Toxicol. Sci."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.1038\/oby.2009.354","article-title":"Prenatal exposure to maternal cigarette smoking and accumulation of intra-abdominal fat during adolescence","volume":"18","author":"Syme","year":"2010","journal-title":"Obesity"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1093\/toxsci\/kfaa127","article-title":"Early Life Exposure to Nicotine: Postnatal Metabolic, Neurobehavioral and Respiratory Outcomes and the Development of Childhood Cancers","volume":"178","author":"Jamshed","year":"2020","journal-title":"Toxicol. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"611384","DOI":"10.3389\/fnbeh.2020.611384","article-title":"Digging Signatures in 13-Month-Old 3xTg-AD Mice for Alzheimer\u2019s Disease and Its Disruption by Isolation Despite Social Life Since They Were Born","volume":"14","year":"2021","journal-title":"Front. Behav. Neurosci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2053","DOI":"10.1097\/00001756-200107030-00052","article-title":"Burrowing into prion disease","volume":"12","author":"Deacon","year":"2001","journal-title":"NeuroReport"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/S0166-4328(01)00401-6","article-title":"Hippocampal cytotoxic lesion effects on species-typical behaviours in mice","volume":"132","author":"Deacon","year":"2002","journal-title":"Behav. Brain Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"112383","DOI":"10.1016\/j.bbr.2019.112383","article-title":"Impaired memory and marble burying activity in deformed epidermal autoregulatory factor 1 (Deaf1) conditional knockout mice","volume":"380","author":"McGee","year":"2020","journal-title":"Behav. Brain Res."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Santana-Santana, M., Bayascas, J.-R., and Gim\u00e9nez-Llort, L. (2021). Sex-Dependent Signatures, Time Frames and Longitudinal Fine-Tuning of the Marble Burying Test in Normal and AD-Pathological Aging Mice. Biomedicines, 9.","DOI":"10.3390\/biomedicines9080994"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.bbr.2011.11.007","article-title":"Lack of cyclin D2 impairing adult brain neurogenesis alters hippocampal-dependent behavioral tasks without reducing learning ability","volume":"227","author":"Jedynak","year":"2012","journal-title":"Behav. Brain Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/0091-3057(91)90590-X","article-title":"Evaluation of marble-burying behavior as a model of anxiety","volume":"38","author":"Handley","year":"1991","journal-title":"Pharmacol. Biochem. Behav."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1038\/nprot.2006.20","article-title":"Digging and marble burying in mice: Simple methods for in vivo identification of biological impacts","volume":"1","author":"Deacon","year":"2006","journal-title":"Nat. Protoc."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Zhang, L., Spencer, T.J., Biederman, J., and Bhide, P.G. (2018). Attention and working memory deficits in a perinatal nicotine exposure mouse model. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0198064"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.brainresbull.2021.07.030","article-title":"Developmental nicotine exposure impairs memory and reduces acetylcholine levels in the hippocampus of mice","volume":"176","author":"Gavini","year":"2021","journal-title":"Brain Res. Bull."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Polli, F.S., Scharff, M.B., Ipsen, T.H., Aznar, S., Kohlmeier, K.A., and Andreasen, J.T. (2020). Prenatal nicotine exposure in mice induces sex-dependent anxiety-like behavior, cognitive deficits, hyperactivity, and changes in the expression of glutamate receptor associated-genes in the prefrontal cortex. Pharmacol. Biochem. Behav., 195.","DOI":"10.1016\/j.pbb.2020.172951"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.ijdevneu.2004.05.009","article-title":"In utero nicotine exposure causes persistent, gender-dependant changes in locomotor activity and sensitivity to nicotine in C57Bl\/6 mice","volume":"22","author":"Pauly","year":"2004","journal-title":"Int. J. Dev. Neurosci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.ejphar.2010.04.044","article-title":"Nicotine exposure throughout early development promotes nicotine self-administration in adolescent mice and induces long-lasting behavioural changes","volume":"640","author":"Chistyakov","year":"2010","journal-title":"Eur. J. Pharmacol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1080\/14622200050011295","article-title":"Prenatal tobacco exposure: Is it a risk factor for early tobacco experimentation?","volume":"2","author":"Cornelius","year":"2000","journal-title":"Nicotine Tob. Res."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1007\/s00213-003-1486-y","article-title":"Adolescent-onset nicotine self-administration modeled in female rats","volume":"169","author":"Levin","year":"2003","journal-title":"Psychopharmacology"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1002\/jnr.23858","article-title":"Sex differences in nicotine preference","volume":"95","author":"Pogun","year":"2017","journal-title":"J. Neurosci. Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1736","DOI":"10.15585\/mmwr.mm6946a4","article-title":"Tobacco Product Use Among Adults\u2014United States, 2019","volume":"69","author":"Cornelius","year":"2020","journal-title":"MMWR Morb. Mortal. Wkly. Rep."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.brainresbull.2015.11.010","article-title":"Targeting glutamate homeostasis for potential treatment of nicotine dependence","volume":"121","author":"Alasmari","year":"2016","journal-title":"Brain Res. Bull."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1002\/(SICI)1098-2396(200002)35:2<129::AID-SYN5>3.0.CO;2-D","article-title":"Nicotine stimulation of extracellular glutamate levels in the nucleus accumbens: Neuropharmacological characterization","volume":"35","author":"Reid","year":"2000","journal-title":"Synapse"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1414","DOI":"10.1111\/j.1530-0277.2006.00169.x","article-title":"Nicotine stimulation on extracellular glutamate levels in the nucleus accumbens of ethanol-withdrawn rats in vivo","volume":"30","author":"Huebner","year":"2006","journal-title":"Alcohol. Clin. Exp. Res."},{"key":"ref_58","unstructured":"Science, A.I.f.B. (2022, March 01). Allen Developing Mouse Brain Atlas 2008. Available online: https:\/\/alleninstitute.org\/division\/brain-science\/."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1909","DOI":"10.1038\/s41386-020-0734-8","article-title":"Validation of a nicotine vapor self-administration model in rats with relevance to electronic cigarette use","volume":"45","author":"Smith","year":"2020","journal-title":"Neuropsychopharmacology"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1124\/pr.116.012484","article-title":"The Nucleus Accumbens: Mechanisms of Addiction across Drug Classes Reflect the Importance of Glutamate Homeostasis","volume":"68","author":"Scofield","year":"2016","journal-title":"Pharmacol. Rev."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Alhaddad, H., Wong, W., Sari, A.T., Crotty Alexander, L.E., and Sari, Y. (2020). Effects of 3-Month Exposure to E-Cigarette Aerosols on Glutamatergic Receptors and Transporters in Mesolimbic Brain Regions of Female C57BL\/6 Mice. Toxics, 8.","DOI":"10.3390\/toxics8040095"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1093\/ntr\/ntp001","article-title":"Association between smoking and retrospectively reported attention-deficit\/hyperactivity disorder symptoms in a large sample of new mothers","volume":"11","author":"Willoughby","year":"2009","journal-title":"Nicotine Tob. Res."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1142","DOI":"10.1001\/archpsyc.62.10.1142","article-title":"Association Between Smoking and Attention-Deficit\/Hyperactivity Disorder Symptoms in a Population-Based Sample of Young Adults","volume":"62","author":"Kollins","year":"2005","journal-title":"Arch. Gen. Psychiatry"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1007\/s12017-019-08578-y","article-title":"Topiramate Reverses Physiological and Behavioral Alterations by Postoperative Cognitive Dysfunction in Rat Model Through Inhibiting TNF Signaling Pathway","volume":"22","author":"Su","year":"2020","journal-title":"Neuromol. Med."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1920","DOI":"10.1007\/s12640-021-00421-2","article-title":"Lacidipine Attenuates Symptoms of Nicotine Withdrawal in Mice","volume":"39","author":"Khurana","year":"2021","journal-title":"Neurotox. Res."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"2989","DOI":"10.1007\/s00213-014-3472-y","article-title":"The effects of lobeline on nicotine withdrawal-induced depression-like behavior in mice","volume":"231","author":"Roni","year":"2014","journal-title":"Psychopharmacology"},{"key":"ref_67","first-page":"2375","article-title":"Enriched environment palliates nicotine-induced addiction and associated neurobehavioral deficits in rats","volume":"30","author":"Nawaz","year":"2017","journal-title":"Pak. J. Pharm. Sci."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1042","DOI":"10.1289\/ehp.0800193","article-title":"Prenatal Exposure to Cigarette Smoke Induces Diet- and Sex-Dependent Dyslipidemia and Weight Gain in Adult Murine Offspring","volume":"117","author":"Ng","year":"2009","journal-title":"Environ. Health Perspect."}],"container-title":["Brain Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3425\/13\/10\/1417\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:01:55Z","timestamp":1760130115000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3425\/13\/10\/1417"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,6]]},"references-count":68,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2023,10]]}},"alternative-id":["brainsci13101417"],"URL":"https:\/\/doi.org\/10.3390\/brainsci13101417","relation":{},"ISSN":["2076-3425"],"issn-type":[{"value":"2076-3425","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,6]]}}}