{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T07:25:10Z","timestamp":1789802710075,"version":"4.0.1"},"reference-count":73,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2024,10,21]],"date-time":"2024-10-21T00:00:00Z","timestamp":1729468800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003443","name":"Ministry of Education and Science of the Russian Federation","doi-asserted-by":"publisher","award":["075-15-2022-264"],"award-info":[{"award-number":["075-15-2022-264"]}],"id":[{"id":"10.13039\/501100003443","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Brain Sciences"],"abstract":"<jats:p>Background\/Objectives: One of the hallmarks of Alzheimer\u2019s disease (AD) is the accumulation of aggregated beta-amyloid (A\u03b2) protein in the form of senile plaques within brain tissue. Senile plaques contain various post-translational modifications of A\u03b2, including prevalent isomerization of Asp7 residue. The Asp7 isomer has been shown to exhibit increased neurotoxicity and induce amyloidogenesis in brain tissue of transgenic mice. The toxicity of A\u03b2 peptides may be partly mediated by their structure and morphology. In this respect, in this study we analyzed the structural and aggregation characteristics of the Asp7 isoform of A\u03b242 and compared them to those of synthetic A\u03b242. We also investigated the effects of intracerebroventricular (i.c.v.) administration of these peptides, a method often used to induce AD-like symptoms in rodent models. Methods: Atomic force microscopy (AFM) was conducted to compare the morphological and aggregation properties of A\u03b242 and Asp7 iso-A\u03b242. The effects of i.c.v. stereotaxic administration of the proteins were assessed via behavioral analysis and reactive oxygen species (ROS) estimation in vivo using a scanning ion-conductance microscope with a confocal module. Results: AFM measurements revealed structural differences between the two peptides, most notably in their soluble toxic oligomeric forms. The i.c.v. administration of Asp7 iso-A\u03b242 induced spatial memory deficits in rats and elevated oxidative stress levels in vivo, suggesting a potential of ROS in the pathogenic mechanism of the peptide. Conclusions: The findings support the further investigation of Asp7 iso-A\u03b242 in translational research on AD and suggest its involvement in neurodegenerative processes.<\/jats:p>","DOI":"10.3390\/brainsci14101042","type":"journal-article","created":{"date-parts":[[2024,10,21]],"date-time":"2024-10-21T10:49:22Z","timestamp":1729507762000},"page":"1042","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Beta-Amyloid and Its Asp7 Isoform: Morphological and Aggregation Properties and Effects of Intracerebroventricular Administration"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3480-910X","authenticated-orcid":false,"given":"Valeriya","family":"Ushakova","sequence":"first","affiliation":[{"name":"Department of Basic and Applied Neurobiology, V.P. Serbsky National Medical Research Center of Psychiatry and Narcology, The Ministry of Health of the Russian Federation, 119034 Moscow, Russia"},{"name":"Department of Higher Nervous Function, Lomonosov Moscow State University, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0247-2717","authenticated-orcid":false,"given":"Yana","family":"Zorkina","sequence":"additional","affiliation":[{"name":"Department of Basic and Applied Neurobiology, V.P. Serbsky National Medical Research Center of Psychiatry and Narcology, The Ministry of Health of the Russian Federation, 119034 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8793-1833","authenticated-orcid":false,"given":"Olga","family":"Abramova","sequence":"additional","affiliation":[{"name":"Department of Basic and Applied Neurobiology, V.P. Serbsky National Medical Research Center of Psychiatry and Narcology, The Ministry of Health of the Russian Federation, 119034 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Regina","family":"Kuanaeva","sequence":"additional","affiliation":[{"name":"Laboratory of Biophysics, National University of Science and Technology \u201cMISIS\u201d, 119049 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evgeny","family":"Barykin","sequence":"additional","affiliation":[{"name":"Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119334 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8201-8498","authenticated-orcid":false,"given":"Alexander","family":"Vaneev","sequence":"additional","affiliation":[{"name":"Laboratory of Biophysics, National University of Science and Technology \u201cMISIS\u201d, 119049 Moscow, Russia"},{"name":"Chemistry Department, Lomonosov Moscow State University, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roman","family":"Timoshenko","sequence":"additional","affiliation":[{"name":"Laboratory of Biophysics, National University of Science and Technology \u201cMISIS\u201d, 119049 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4860-9013","authenticated-orcid":false,"given":"Peter","family":"Gorelkin","sequence":"additional","affiliation":[{"name":"Laboratory of Biophysics, National University of Science and Technology \u201cMISIS\u201d, 119049 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Erofeev","sequence":"additional","affiliation":[{"name":"Laboratory of Biophysics, National University of Science and Technology \u201cMISIS\u201d, 119049 Moscow, Russia"},{"name":"Chemistry Department, Lomonosov Moscow State University, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugene","family":"Zubkov","sequence":"additional","affiliation":[{"name":"Department of Basic and Applied Neurobiology, V.P. Serbsky National Medical Research Center of Psychiatry and Narcology, The Ministry of Health of the Russian Federation, 119034 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8988-3417","authenticated-orcid":false,"given":"Marat","family":"Valikhov","sequence":"additional","affiliation":[{"name":"Department of Basic and Applied Neurobiology, V.P. Serbsky National Medical Research Center of Psychiatry and Narcology, The Ministry of Health of the Russian Federation, 119034 Moscow, Russia"},{"name":"Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119334 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"Gurina","sequence":"additional","affiliation":[{"name":"Department of Basic and Applied Neurobiology, V.P. Serbsky National Medical Research Center of Psychiatry and Narcology, The Ministry of Health of the Russian Federation, 119034 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1517-1983","authenticated-orcid":false,"given":"Vladimir","family":"Mitkevich","sequence":"additional","affiliation":[{"name":"Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119334 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir","family":"Chekhonin","sequence":"additional","affiliation":[{"name":"Department of Basic and Applied Neurobiology, V.P. Serbsky National Medical Research Center of Psychiatry and Narcology, The Ministry of Health of the Russian Federation, 119034 Moscow, Russia"},{"name":"Department of Medical Nanobiotechnology, N. I. Pirogov Russian National Research Medical University, the Ministry of Health of the Russian Federation, 117513 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Morozova","sequence":"additional","affiliation":[{"name":"Department of Basic and Applied Neurobiology, V.P. Serbsky National Medical Research Center of Psychiatry and Narcology, The Ministry of Health of the Russian Federation, 119034 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,21]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2024, May 15). 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