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However, the exact mechanism governing referred somatic hyperalgesia remains elusive. Methods: By injecting 20% acetic acid into the stomach, we established a mouse model of gastric ulcer (GU). Hematoxylin and eosin (H&amp;E) staining was used as the evaluation criterion for the gastric ulcer model. Evan\u2019s blue (EB) and von Frey tests detected the somatic sensitized area. The DRG neurons distributed among the spinal segments of the sensitized area were prepared for biochemical and electrophysiological experiments. The CCR2 antagonist was intraperitoneally (i.p.) injected into GU mice to test the effect of blocking CCR2 on somatic neurogenic inflammation. Results: GU not only instigated neurogenic plasma extravasation and referred somatic allodynia in the upper back regions spanning the T9 to T11 segments but also augmented the co-expression of T-type Ca2+ channels and CCR2 and led to the gating properties of T-type Ca2+ channel alteration in T9\u2013T11 small-diameter DRG neurons. Moreover, the administration of the CCR2 antagonist inhibited the T-type Ca2+ channel activation, consequently mitigating neurogenic inflammation and referred somatic hyperalgesia. The application of the CCR2 agonist to normal T9\u2013T11 small-diameter DRG neurons simulates the changes in the gating properties of T-type Ca2+ channel that occur in the GU group. Conclusions: Therefore, these findings indicate that CCR2 may function as a critical regulator in the generation of neurogenic inflammation and mechanical allodynia by modulating the gating properties of the T-type Ca2+ channels.<\/jats:p>","DOI":"10.3390\/brainsci15030255","type":"journal-article","created":{"date-parts":[[2025,2,28]],"date-time":"2025-02-28T04:28:18Z","timestamp":1740716898000},"page":"255","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["CCR2 Regulates Referred Somatic Hyperalgesia by Mediating T-Type Ca2+ Channel Currents of Small-Diameter DRG Neurons in Gastric Ulcer Mice"],"prefix":"10.3390","volume":"15","author":[{"given":"Ziyan","family":"Yuan","sequence":"first","affiliation":[{"name":"Shaanxi Key Laboratory of Integrative Acupuncture and Medicine, Shaanxi University of Chinese Medicine, Xianyang 712046, China"},{"name":"Key Laboratory of Acupuncture and Neurobiology, Shaanxi Administration of Traditional Chinese Medicine, Xianyang 712046, China"},{"name":"College of Acupuncture-Moxibustion and Tuina, Shaanxi University of Chinese Medicine, Xianyang 712046, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huanhuan","family":"Liu","sequence":"additional","affiliation":[{"name":"Shaanxi Key Laboratory of Integrative Acupuncture and Medicine, Shaanxi University of Chinese Medicine, Xianyang 712046, China"},{"name":"Key Laboratory of Acupuncture and Neurobiology, Shaanxi Administration of Traditional Chinese Medicine, Xianyang 712046, China"},{"name":"College of Acupuncture-Moxibustion and Tuina, Shaanxi University of Chinese Medicine, Xianyang 712046, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhijun","family":"Diao","sequence":"additional","affiliation":[{"name":"Shaanxi Key Laboratory of Integrative Acupuncture and Medicine, Shaanxi University of Chinese Medicine, Xianyang 712046, China"},{"name":"Key Laboratory of Acupuncture and Neurobiology, Shaanxi Administration of Traditional Chinese Medicine, Xianyang 712046, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Yuan","sequence":"additional","affiliation":[{"name":"College of Acupuncture-Moxibustion and Tuina, Shaanxi University of Chinese Medicine, Xianyang 712046, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuwei","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Acupuncture-Moxibustion and Tuina, Shaanxi University of Chinese Medicine, Xianyang 712046, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simeng","family":"Xue","sequence":"additional","affiliation":[{"name":"Shaanxi Key Laboratory of Integrative Acupuncture and Medicine, Shaanxi University of Chinese Medicine, Xianyang 712046, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinyan","family":"Gao","sequence":"additional","affiliation":[{"name":"College of Acupuncture-Moxibustion and Tuina, Shaanxi University of Chinese Medicine, Xianyang 712046, China"},{"name":"Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haifa","family":"Qiao","sequence":"additional","affiliation":[{"name":"Shaanxi Key Laboratory of Integrative Acupuncture and Medicine, Shaanxi University of Chinese Medicine, Xianyang 712046, China"},{"name":"Shaanxi Collaborative Innovation Center of TCM Technologies and Devices, Shaanxi University of Chinese Medicine, Xianyang 712046, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Jin, Q., Chang, Y., Lu, C., Chen, L., and Wang, Y. 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