{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T17:09:22Z","timestamp":1785431362231,"version":"3.56.0"},"reference-count":141,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2026,4,19]],"date-time":"2026-04-19T00:00:00Z","timestamp":1776556800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["24CDA1269588"],"award-info":[{"award-number":["24CDA1269588"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"award":["24CDA1269588"],"award-info":[{"award-number":["24CDA1269588"]}],"id":[{"id":"https:\/\/ror.org\/013kjyp64","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Brain Sciences"],"abstract":"<jats:p>Brain organoids represent three-dimensional structures that allow for human-specific studies in brain development, pathology and therapeutics. These self-organizing systems, formed through the differentiation of human pluripotent stem cells, can mimic important cellular and molecular events of brain development and therefore serve as a platform for the investigation of neurodevelopmental and neurodegenerative diseases, brain injuries, and tumorigenesis. Although brain organoids show promising perspectives in the study of human physiology, existing brain organoid platforms are hindered by issues of under vascularization, immaturity and protocol variability. Nevertheless, the rapid development of new bioengineering, microfluidic and multi-omics tools and approaches allows us to overcome existing problems and increase the physiological significance of these organoids. Brain organoid transplantation and functional studies further enhance the applications of brain organoids in drug screening, disease modeling and personalized medicine. Here, we provide an overview of recent developments in the field of brain organoid cultures, functional characteristics and translational applications.<\/jats:p>","DOI":"10.3390\/brainsci16040427","type":"journal-article","created":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T09:37:42Z","timestamp":1776677862000},"page":"427","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Brain Organoids: Emerging Platforms for Modern Neuroscience"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0004-9440","authenticated-orcid":false,"given":"Lian","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Neurology, Institute for Cerebrovascular and Neuroregeneration Research (ICNR), Louisiana State University Health Sciences Center, 1501 Kings Highway, Shreveport, LA 71103, USA"},{"name":"Department of Neurology, Medical College of Georgia, Augusta University, 1120 15th Street, Augusta, GA 30912, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1406-8183","authenticated-orcid":false,"given":"Liwei","family":"Mao","sequence":"additional","affiliation":[{"name":"Department of Neurology, Institute for Cerebrovascular and Neuroregeneration Research (ICNR), Louisiana State University Health Sciences Center, 1501 Kings Highway, Shreveport, LA 71103, USA"},{"name":"Department of Neurology, Medical College of Georgia, Augusta University, 1120 15th Street, Augusta, GA 30912, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Neurology, David Geffen School of Medicine, University of California, 710 Westwood Plaza, Los Angeles, CA 90095, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0010-1664","authenticated-orcid":false,"given":"Xuemei","family":"Zong","sequence":"additional","affiliation":[{"name":"Department of Neurology, Institute for Cerebrovascular and Neuroregeneration Research (ICNR), Louisiana State University Health Sciences Center, 1501 Kings Highway, Shreveport, LA 71103, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"20417314231226027","DOI":"10.1177\/20417314231226027","article-title":"Efficient generation of human cerebral organoids directly from adherent cultures of pluripotent stem cells","volume":"15","author":"Coronel","year":"2024","journal-title":"J. Tissue Eng."},{"key":"ref_2","first-page":"1055","article-title":"A facile method to generate cerebral organoids from human pluripotent stem cells","volume":"22","author":"Simorgh","year":"2023","journal-title":"Excli J."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"e202000707","DOI":"10.26508\/lsa.202000707","article-title":"Robust production of uniform human cerebral organoids from pluripotent stem cells","volume":"3","author":"Sivitilli","year":"2020","journal-title":"Life Sci. Alliance"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2712","DOI":"10.1038\/s41596-024-00994-0","article-title":"Generation of \u2018semi-guided\u2019 cortical organoids with complex neural oscillations","volume":"19","author":"Fitzgerald","year":"2024","journal-title":"Nat. Protoc."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"105140","DOI":"10.1016\/j.isci.2022.105140","article-title":"Human PSCs determine the competency of cerebral organoid differentiation via FGF signaling and epigenetic mechanisms","volume":"25","author":"Ideno","year":"2022","journal-title":"iScience"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Eigenhuis, K.N., Somsen, H.B., van der Kroeg, M., Smeenk, H., Korporaal, A.L., Kushner, S.A., de Vrij, F.M.S., and van den Berg, D.L.C. (2023). A simplified protocol for the generation of cortical brain organoids. Front. Cell. Neurosci., 17.","DOI":"10.3389\/fncel.2023.1114420"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2329","DOI":"10.1038\/nprot.2014.158","article-title":"Generation of cerebral organoids from human pluripotent stem cells","volume":"9","author":"Lancaster","year":"2014","journal-title":"Nat. Protoc."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"dev200561","DOI":"10.1242\/dev.200561","article-title":"Rapid and robust directed differentiation of mouse epiblast stem cells into definitive endoderm and forebrain organoids","volume":"149","author":"Corrigan","year":"2022","journal-title":"Development"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"4000","DOI":"10.1038\/s41596-020-0402-9","article-title":"Generation and biobanking of patient-derived glioblastoma organoids and their application in CAR T cell testing","volume":"15","author":"Jacob","year":"2020","journal-title":"Nat. Protoc."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2827","DOI":"10.1038\/s41596-018-0066-x","article-title":"Blood-brain-barrier organoids for investigating the permeability of CNS therapeutics","volume":"13","author":"Bergmann","year":"2018","journal-title":"Nat. Protoc."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1038\/s41593-019-0350-2","article-title":"Cerebral organoids at the air-liquid interface generate diverse nerve tracts with functional output","volume":"22","author":"Giandomenico","year":"2019","journal-title":"Nat. Neurosci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"109415","DOI":"10.1016\/j.isci.2024.109415","article-title":"Cortical brain organoid slices (cBOS) for the study of human neural cells in minimal networks","volume":"27","author":"Petersilie","year":"2024","journal-title":"iScience"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1038\/nprot.2017.152","article-title":"Generation of human brain region-specific organoids using a miniaturized spinning bioreactor","volume":"13","author":"Qian","year":"2018","journal-title":"Nat. Protoc."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1089\/ten.tea.2016.0400","article-title":"Derivation of Cortical Spheroids from Human Induced Pluripotent Stem Cells in a Suspension Bioreactor","volume":"24","author":"Yan","year":"2018","journal-title":"Tissue Eng. Part A"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1038\/s42003-023-04547-1","article-title":"Spatio-temporal dynamics enhance cellular diversity, neuronal function and further maturation of human cerebral organoids","volume":"6","author":"Devamoglu","year":"2023","journal-title":"Commun. Biol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"e52904","DOI":"10.7554\/eLife.52904","article-title":"A fully automated high-throughput workflow for 3D-based chemical screening in human midbrain organoids","volume":"9","author":"Renner","year":"2020","journal-title":"eLife"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"015014","DOI":"10.1088\/1758-5090\/ad0c2c","article-title":"Modular 3D printed platform for fluidically connected human brain organoid culture","volume":"16","author":"Rezaei","year":"2023","journal-title":"Biofabrication"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"970","DOI":"10.5966\/sctm.2015-0305","article-title":"Rapid Induction of Cerebral Organoids From Human Induced Pluripotent Stem Cells Using a Chemically Defined Hydrogel and Defined Cell Culture Medium","volume":"5","author":"Lindborg","year":"2016","journal-title":"Stem Cells Transl. Med."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"114874","DOI":"10.1016\/j.celrep.2024.114874","article-title":"Biofunctionalized gelatin hydrogels support development and maturation of iPSC-derived cortical organoids","volume":"43","author":"Kjar","year":"2024","journal-title":"Cell Rep."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Tejchman, A., Zn\u00f3j, A., Chlebanowska, P., Fr\u0105czek-Szczypta, A., and Majka, M. (2020). Carbon Fibers as a New Type of Scaffold for Midbrain Organoid Development. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21175959"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2041731416671926","DOI":"10.1177\/2041731416671926","article-title":"Multi-compartmental biomaterial scaffolds for patterning neural tissue organoids in models of neurodevelopment and tissue regeneration","volume":"7","author":"McMurtrey","year":"2016","journal-title":"J. Tissue Eng."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Balion, Z., Svirskien\u0117, N., Svirskis, G., Inokaitis, H., C\u0117pla, V., Ul\u010dinas, A., Jelinskas, T., Eimont, R., Pau\u017eien\u0117, N., and Valiokas, R. (2022). Comparison of Microglial Morphology and Function in Primary Cerebellar Cell Cultures on Collagen and Collagen-Mimetic Hydrogels. Biomedicines, 10.","DOI":"10.3390\/biomedicines10051023"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Balion, Z., C\u0117pla, V., Svirskiene, N., Svirskis, G., Druceikait\u0117, K., Inokaitis, H., Rusteikait\u0117, J., Masilionis, I., Stankevi\u010dien\u0117, G., and Jelinskas, T. (2020). Cerebellar Cells Self-Assemble into Functional Organoids on Synthetic, Chemically Crosslinked ECM-Mimicking Peptide Hydrogels. Biomolecules, 10.","DOI":"10.3390\/biom10050754"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"872","DOI":"10.1016\/j.cels.2023.09.004","article-title":"Volumetric compression by heterogeneous scaffold embedding promotes cerebral organoid maturation and does not impede growth","volume":"14","author":"Tang","year":"2023","journal-title":"Cell Syst."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Sozzi, E., Kajtez, J., Bruzelius, A., Wesseler, M.F., Nilsson, F., Birtele, M., Larsen, N.B., Ottosson, D.R., Storm, P., and Parmar, M. (2022). Silk scaffolding drives self-assembly of functional and mature human brain organoids. Front. Cell Dev. Biol., 10.","DOI":"10.3389\/fcell.2022.1023279"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e2400070","DOI":"10.1002\/biot.202400070","article-title":"Microfabricated dynamic brain organoid cocultures to assess the effects of surface geometry on assembloid formation","volume":"19","author":"Rostami","year":"2024","journal-title":"Biotechnol. J."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1421","DOI":"10.1038\/s41587-020-00763-w","article-title":"Generation of human striatal organoids and cortico-striatal assembloids from human pluripotent stem cells","volume":"38","author":"Miura","year":"2020","journal-title":"Nat. Biotechnol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.stem.2024.11.005","article-title":"Generation of self-organized neuromusculoskeletal tri-tissue organoids from human pluripotent stem cells","volume":"32","author":"Yin","year":"2025","journal-title":"Cell Stem Cell"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"5977","DOI":"10.1038\/s41598-019-42439-9","article-title":"Assembly of Human Stem Cell-Derived Cortical Spheroids and Vascular Spheroids to Model 3-D Brain-like Tissues","volume":"9","author":"Song","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"118021","DOI":"10.1016\/j.biopha.2025.118021","article-title":"Multi-organ model assessment of neurotoxicity following exposure of liver spheroids to drugs","volume":"186","author":"Wu","year":"2025","journal-title":"Biomed. Pharmacother."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.bbrc.2023.03.019","article-title":"Modeling the marmoset brain using embryonic stem cell-derived cerebral assembloids","volume":"657","author":"Kodera","year":"2023","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2062","DOI":"10.1038\/s41596-018-0032-7","article-title":"Generation and assembly of human brain region-specific three-dimensional cultures","volume":"13","author":"Sloan","year":"2018","journal-title":"Nat. Protoc."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"025005","DOI":"10.1088\/1758-5090\/ad1b1e","article-title":"Uniform cerebral organoid culture on a pillar plate by simple and reproducible spheroid transfer from an ultralow attachment well plate","volume":"16","author":"Acharya","year":"2024","journal-title":"Biofabrication"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2941","DOI":"10.1039\/C7LC00682A","article-title":"In situ generation of human brain organoids on a micropillar array","volume":"17","author":"Zhu","year":"2017","journal-title":"Lab Chip"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1677","DOI":"10.1039\/C7RA11714K","article-title":"Engineering stem cell-derived 3D brain organoids in a perfusable organ-on-a-chip system","volume":"8","author":"Wang","year":"2018","journal-title":"RSC Adv."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.jbiotec.2010.02.001","article-title":"Design and prototyping of a chip-based multi-micro-organoid culture system for substance testing, predictive to human (substance) exposure","volume":"148","author":"Sonntag","year":"2010","journal-title":"J. Biotechnol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"e2400240","DOI":"10.1002\/biot.202400240","article-title":"A closed 3D printed microfluidic device for automated growth and differentiation of cerebral organoids from single-cell suspension","volume":"19","author":"Kandra","year":"2024","journal-title":"Biotechnol. J."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"20173","DOI":"10.1038\/s41598-022-20096-9","article-title":"Modular automated microfluidic cell culture platform reduces glycolytic stress in cerebral cortex organoids","volume":"12","author":"Seiler","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"024105","DOI":"10.1063\/5.0041027","article-title":"A low-cost 3D printed microfluidic bioreactor and imaging chamber for live-organoid imaging","volume":"15","author":"Khan","year":"2021","journal-title":"Biomicrofluidics"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"698","DOI":"10.1038\/s41556-024-01393-z","article-title":"A cell fate decision map reveals abundant direct neurogenesis bypassing intermediate progenitors in the human developing neocortex","volume":"26","author":"Coquand","year":"2024","journal-title":"Nat. Cell Biol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1016\/j.stemcr.2020.12.019","article-title":"Cell-to-Cell Adhesion and Neurogenesis in Human Cortical Development: A Study Comparing 2D Monolayers with 3D Organoid Cultures","volume":"16","author":"Scuderi","year":"2021","journal-title":"Stem Cell Rep."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"eabl6422","DOI":"10.1126\/science.abl6422","article-title":"Human TKTL1 implies greater neurogenesis in frontal neocortex of modern humans than Neanderthals","volume":"377","author":"Pinson","year":"2022","journal-title":"Science"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1016\/j.stem.2023.01.007","article-title":"Epitranscriptomic regulation of cortical neurogenesis via Mettl8-dependent mitochondrial tRNA m3C modification","volume":"30","author":"Zhang","year":"2023","journal-title":"Cell Stem Cell"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.devcel.2023.11.021","article-title":"Lysosomal dynamics regulate mammalian cortical neurogenesis","volume":"59","author":"Zou","year":"2024","journal-title":"Dev. Cell"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"dev185132","DOI":"10.1242\/dev.185132","article-title":"Heterogeneous nuclear ribonucleoprotein A3 controls mitotic progression of neural progenitors via interaction with cohesin","volume":"147","author":"Ou","year":"2020","journal-title":"Development"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1016\/j.stem.2025.02.011","article-title":"m6A deficiency impairs hypothalamic neurogenesis of feeding-related neurons in mice and human organoids and leads to adult obesity in mice","volume":"32","author":"Shen","year":"2025","journal-title":"Cell Stem Cell"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"e13732","DOI":"10.1111\/cpr.13732","article-title":"Flavonoid chrysin activates both TrkB and FGFR1 receptors while upregulates their endogenous ligands such as brain derived neurotrophic factor to promote human neurogenesis","volume":"58","author":"Dong","year":"2025","journal-title":"Cell Prolif."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1093\/stmcls\/sxac046","article-title":"\u03b2-Arrestin 2 and Epac2 Cooperatively Mediate DRD1-Stimulated Proliferation of Human Neural Stem Cells and Growth of Human Cerebral Organoids","volume":"40","author":"Dong","year":"2022","journal-title":"Stem Cells"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1002\/stem.3156","article-title":"Constitutive activity of a G protein-coupled receptor, DRD1, contributes to human cerebral organoid formation","volume":"38","author":"Wang","year":"2020","journal-title":"Stem Cells"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1016\/j.cell.2018.06.007","article-title":"Evolution of Cortical Neurogenesis in Amniotes Controlled by Robo Signaling Levels","volume":"174","author":"Villalba","year":"2018","journal-title":"Cell"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1186\/s12951-025-03298-x","article-title":"Development of NIR photocleavable nanoparticles with BDNF for vestibular neuron regeneration","volume":"23","author":"Abueva","year":"2025","journal-title":"J. Nanobiotechnol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1038\/s41419-022-04693-0","article-title":"Deficiency of N-glycanase 1 perturbs neurogenesis and cerebral development modeled by human organoids","volume":"13","author":"Lin","year":"2022","journal-title":"Cell Death Dis."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"6617","DOI":"10.1038\/s41598-021-84278-7","article-title":"PINK1 deficiency impairs adult neurogenesis of dopaminergic neurons","volume":"11","author":"Brown","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1038\/s41467-019-14235-6","article-title":"FICD activity and AMPylation remodelling modulate human neurogenesis","volume":"11","author":"Kielkowski","year":"2020","journal-title":"Nat. Commun."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"e54728","DOI":"10.15252\/embr.202254728","article-title":"Human-specific ARHGAP11B ensures human-like basal progenitor levels in hominid cerebral organoids","volume":"23","author":"Fischer","year":"2022","journal-title":"EMBO Rep."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"eads7543","DOI":"10.1126\/sciadv.ads7543","article-title":"A dyad of human-specific NBPF14 and NOTCH2NLB orchestrates cortical progenitor abundance crucial for human neocortex expansion","volume":"11","author":"Liutikaite","year":"2025","journal-title":"Sci. Adv."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1388","DOI":"10.1038\/s44318-024-00068-7","article-title":"The growth factor EPIREGULIN promotes basal progenitor cell proliferation in the developing neocortex","volume":"43","author":"Cubillos","year":"2024","journal-title":"Embo J."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"2847","DOI":"10.1016\/j.neuron.2021.07.007","article-title":"Proneural genes define ground-state rules to regulate neurogenic patterning and cortical folding","volume":"109","author":"Han","year":"2021","journal-title":"Neuron"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/j.stem.2016.11.017","article-title":"Induction of Expansion and Folding in Human Cerebral Organoids","volume":"20","author":"Li","year":"2017","journal-title":"Cell Stem Cell"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1038\/s41420-023-01774-7","article-title":"Apically localized PANX1 impacts neuroepithelial expansion in human cerebral organoids","volume":"10","author":"Noort","year":"2024","journal-title":"Cell Death Discov."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"6298","DOI":"10.1038\/s41467-021-26447-w","article-title":"Extracellular LGALS3BP regulates neural progenitor position and relates to human cortical complexity","volume":"12","author":"Kyrousi","year":"2021","journal-title":"Nat. Commun."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"2757","DOI":"10.1140\/epjst\/e2020-000001-6","article-title":"Folding drives cortical thickness variations","volume":"229","author":"Holland","year":"2020","journal-title":"Eur. Phys. J. Spec. Top."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1038\/s41567-018-0046-7","article-title":"Human Brain Organoids on a Chip Reveal the Physics of Folding","volume":"14","author":"Karzbrun","year":"2018","journal-title":"Nat. Phys."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"7361","DOI":"10.1038\/s41467-023-43141-1","article-title":"Temporal morphogen gradient-driven neural induction shapes single expanded neuroepithelium brain organoids with enhanced cortical identity","volume":"14","author":"Pagliaro","year":"2023","journal-title":"Nat. Commun."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"2420","DOI":"10.4103\/1673-5374.335789","article-title":"Engineering cerebral folding in brain organoids","volume":"17","author":"Scott","year":"2022","journal-title":"Neural Regen. Res."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"2945","DOI":"10.1038\/s41467-024-46787-7","article-title":"Complex activity and short-term plasticity of human cerebral organoids reciprocally connected with axons","volume":"15","author":"Osaki","year":"2024","journal-title":"Nat. Commun."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"114503","DOI":"10.1016\/j.celrep.2024.114503","article-title":"Synaptic plasticity in human thalamocortical assembloids","volume":"43","author":"Patton","year":"2024","journal-title":"Cell Rep."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"4318","DOI":"10.1007\/s12035-023-03812-y","article-title":"Pathological Deficit of Cystatin B Impairs Synaptic Plasticity in EPM1 Human Cerebral Organoids","volume":"61","author":"Pizzella","year":"2024","journal-title":"Mol. Neurobiol."},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Penna, E., Cerciello, A., Chambery, A., Russo, R., Cernilogar, F.M., Pedone, E.M., Perrone-Capano, C., Cappello, S., Di Giaimo, R., and Crispino, M. (2019). Cystatin B Involvement in Synapse Physiology of Rodent Brains and Human Cerebral Organoids. Front. Mol. Neurosci., 12.","DOI":"10.3389\/fnmol.2019.00195"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"eadj6603","DOI":"10.1126\/scisignal.adj6603","article-title":"Inhibiting Hippo pathway kinases releases WWC1 to promote AMPAR-dependent synaptic plasticity and long-term memory in mice","volume":"17","author":"Stepan","year":"2024","journal-title":"Sci. Signal"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"242","DOI":"10.4103\/NRR.NRR-D-23-00928","article-title":"Establishment of human cerebral organoid systems to model early neural development and assess the central neurotoxicity of environmental toxins","volume":"20","author":"Hu","year":"2025","journal-title":"Neural Regen. Res."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"124913","DOI":"10.1016\/j.envpol.2024.124913","article-title":"PM2.5 induces developmental neurotoxicity in cortical organoids","volume":"361","author":"Han","year":"2024","journal-title":"Environ. Pollut."},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Zhong, X., Harris, G., Smirnova, L., Zufferey, V., S\u00e1, R., Baldino Russo, F., Baleeiro Beltrao Braga, P.C., Chesnut, M., Zurich, M.G., and Hogberg, H.T. (2020). Antidepressant Paroxetine Exerts Developmental Neurotoxicity in an iPSC-Derived 3D Human Brain Model. Front. Cell. Neurosci., 14.","DOI":"10.3389\/fncel.2020.00025"},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Scholz, S., Lewis, K., Saulich, F., Endres, M., Boehmerle, W., and Huehnchen, P. (2022). Induced pluripotent stem cell-derived brain organoids as potential human model system for chemotherapy induced CNS toxicity. Front. Mol. Biosci., 9.","DOI":"10.3389\/fmolb.2022.1006497"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.taap.2018.02.003","article-title":"Rotenone exerts developmental neurotoxicity in a human brain spheroid model","volume":"354","author":"Pamies","year":"2018","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"3373","DOI":"10.1093\/brain\/awad058","article-title":"GGC repeat expansion in NOTCH2NLC induces dysfunction in ribosome biogenesis and translation","volume":"146","author":"Fan","year":"2023","journal-title":"Brain"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1038\/s41586-024-07578-8","article-title":"Brain Chimeroids reveal individual susceptibility to neurotoxic triggers","volume":"631","author":"Faravelli","year":"2024","journal-title":"Nature"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"e2000825","DOI":"10.1002\/adhm.202000825","article-title":"Engineered Perineural Vascular Plexus for Modeling Developmental Toxicity","volume":"9","author":"Kaushik","year":"2020","journal-title":"Adv. Healthc. Mater."},{"key":"ref_79","doi-asserted-by":"crossref","unstructured":"Daneshgar, N., Leidinger, M.R., Le, S., Hefti, M., Prigione, A., and Dai, D.F. (2022). Activated microglia and neuroinflammation as a pathogenic mechanism in Leigh syndrome. Front. Neurosci., 16.","DOI":"10.3389\/fnins.2022.1068498"},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Pranty, A.I., Wruck, W., and Adjaye, J. (2023). Free Bilirubin Induces Neuro-Inflammation in an Induced Pluripotent Stem Cell-Derived Cortical Organoid Model of Crigler-Najjar Syndrome. Cells, 12.","DOI":"10.1101\/2023.07.12.548684"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.1038\/s41380-020-0676-x","article-title":"Glial cell diversity and methamphetamine-induced neuroinflammation in human cerebral organoids","volume":"26","author":"Dang","year":"2021","journal-title":"Mol. Psychiatry"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"eabn2375","DOI":"10.1126\/scitranslmed.abn2375","article-title":"MECP2-related pathways are dysregulated in a cortical organoid model of myotonic dystrophy","volume":"14","author":"Morelli","year":"2022","journal-title":"Sci. Transl. Med."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"1888","DOI":"10.1038\/s41591-020-1043-9","article-title":"Neuronal defects in a human cellular model of 22q11.2 deletion syndrome","volume":"26","author":"Khan","year":"2020","journal-title":"Nat. Med."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/j.stem.2021.11.011","article-title":"Dissecting the molecular basis of human interneuron migration in forebrain assembloids from Timothy syndrome","volume":"29","author":"Birey","year":"2022","journal-title":"Cell Stem Cell"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"e2305555","DOI":"10.1002\/advs.202305555","article-title":"Multi-Sensor Origami Platform: A Customizable System for Obtaining Spatiotemporally Precise Functional Readouts in 3D Models","volume":"11","author":"Rahav","year":"2024","journal-title":"Adv. Sci."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"3603","DOI":"10.1039\/D3LC00294B","article-title":"Protruding cantilever microelectrode array to monitor the inner electrical activity of cerebral organoids","volume":"23","author":"Phouphetlinthong","year":"2023","journal-title":"Lab Chip"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"20420","DOI":"10.1038\/s41598-022-24350-y","article-title":"A minimal-complexity light-sheet microscope maps network activity in 3D neuronal systems","volume":"12","author":"Wysmolek","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_88","doi-asserted-by":"crossref","unstructured":"Bozhko, D.V., Galumov, G.K., Polovian, A.I., Kolchanova, S.M., Myrov, V.O., Stelmakh, V.A., and Schi\u00f6th, H.B. (2020). BCNNM: A Framework for in silico Neural Tissue Development Modeling. Front. Comput. Neurosci., 14.","DOI":"10.3389\/fncom.2020.588224"},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"1737","DOI":"10.1038\/s41467-025-56998-1","article-title":"Neuronal hyperactivity in neurons derived from individuals with gray matter heterotopia","volume":"16","author":"Bonrath","year":"2025","journal-title":"Nat. Commun."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1038\/nature22047","article-title":"Cell diversity and network dynamics in photosensitive human brain organoids","volume":"545","author":"Quadrato","year":"2017","journal-title":"Nature"},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1038\/s41390-021-01922-8","article-title":"KIF5C deficiency causes abnormal cortical neuronal migration, dendritic branching, and spine morphology in mice","volume":"92","author":"Li","year":"2022","journal-title":"Pediatr. Res."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"108802","DOI":"10.1016\/j.celrep.2021.108802","article-title":"Transcriptional networks identify synaptotagmin-like 3 as a regulator of cortical neuronal migration during early neurodevelopment","volume":"34","author":"Dong","year":"2021","journal-title":"Cell Rep."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"103140","DOI":"10.1016\/j.isci.2021.103140","article-title":"Restoration of the defect in radial glial fiber migration and cortical plate organization in a brain organoid model of Fukuyama muscular dystrophy","volume":"24","author":"Maruyama","year":"2021","journal-title":"iScience"},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.jneumeth.2017.07.004","article-title":"Visualization of migration of human cortical neurons generated from induced pluripotent stem cells","volume":"289","author":"Bamba","year":"2017","journal-title":"J. Neurosci. Methods"},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1038\/s41586-021-04330-4","article-title":"Androgens increase excitatory neurogenic potential in human brain organoids","volume":"602","author":"Kelava","year":"2022","journal-title":"Nature"},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.neuroscience.2021.12.026","article-title":"N-methyl-d-aspartate Receptor-mediated Preconditioning Mitigates Excitotoxicity in Human Induced Pluripotent Stem Cell-derived Brain Organoids","volume":"484","author":"Bauersachs","year":"2022","journal-title":"Neuroscience"},{"key":"ref_97","doi-asserted-by":"crossref","unstructured":"Posadas, I., Romero-Castillo, L., Ronca, R.A., Karpus, A., Mignani, S., Majoral, J.P., Mu\u00f1oz-Fern\u00e1ndez, M., and Ce\u00f1a, V. (2022). Engineered Neutral Phosphorous Dendrimers Protect Mouse Cortical Neurons and Brain Organoids from Excitotoxic Death. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23084391"},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1186\/s13041-021-00864-w","article-title":"Neuronal excitatory-to-inhibitory balance is altered in cerebral organoid models of genetic neurological diseases","volume":"14","author":"Foliaki","year":"2021","journal-title":"Mol. Brain"},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1038\/s41556-024-01412-z","article-title":"Cerebral organoids display dynamic clonal growth and tunable tissue replenishment","volume":"26","author":"Lindenhofer","year":"2024","journal-title":"Nat. Cell Biol."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1038\/s41592-021-01344-8","article-title":"Lineage recording in human cerebral organoids","volume":"19","author":"He","year":"2022","journal-title":"Nat. Methods"},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"e2309292","DOI":"10.1002\/advs.202309292","article-title":"Genetic Screening of Haploid Neural Stem Cells Reveals that Nfkbia and Atp2b4 are Key Regulators of Oxidative Stress in Neural Precursors","volume":"11","author":"Nie","year":"2024","journal-title":"Adv. Sci."},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"114862","DOI":"10.1016\/j.celrep.2024.114862","article-title":"Neuronal lineage tracing from progenitors in human cortical organoids reveals mechanisms of neuronal production, diversity, and disease","volume":"43","author":"Bury","year":"2024","journal-title":"Cell Rep."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"15672","DOI":"10.1073\/pnas.1520760112","article-title":"Human cerebral organoids recapitulate gene expression programs of fetal neocortex development","volume":"112","author":"Camp","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"eaay1645","DOI":"10.1126\/science.aay1645","article-title":"Chromatin accessibility dynamics in a model of human forebrain development","volume":"367","author":"Trevino","year":"2020","journal-title":"Science"},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"011001","DOI":"10.1088\/1758-5090\/abc95e","article-title":"Next generation human brain models: Engineered flat brain organoids featuring gyrification","volume":"13","author":"Pereira","year":"2021","journal-title":"Biofabrication"},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"025017","DOI":"10.1088\/1758-5090\/ab6d36","article-title":"Drug compound screening in single and integrated multi-organoid body-on-a-chip systems","volume":"12","author":"Skardal","year":"2020","journal-title":"Biofabrication"},{"key":"ref_107","doi-asserted-by":"crossref","unstructured":"Maloney, E., Clark, C., Sivakumar, H., Yoo, K., Aleman, J., Rajan, S.A.P., Forsythe, S., Mazzocchi, A., Laxton, A.W., and Tatter, S.B. (2020). Immersion Bioprinting of Tumor Organoids in Multi-Well Plates for Increasing Chemotherapy Screening Throughput. Micromachines, 11.","DOI":"10.3390\/mi11020208"},{"key":"ref_108","doi-asserted-by":"crossref","unstructured":"Lenin, S., Ponthier, E., Scheer, K.G., Yeo, E.C.F., Tea, M.N., Ebert, L.M., Oksdath Mansilla, M., Poonnoose, S., Baumgartner, U., and Day, B.W. (2021). A Drug Screening Pipeline Using 2D and 3D Patient-Derived In Vitro Models for Pre-Clinical Analysis of Therapy Response in Glioblastoma. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22094322"},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.1093\/neuonc\/noab001","article-title":"PTEN deficiency leads to proteasome addiction: A novel vulnerability in glioblastoma","volume":"23","author":"Benitez","year":"2021","journal-title":"Neuro Oncol."},{"key":"ref_110","doi-asserted-by":"crossref","unstructured":"Ratliff, M., Kim, H., Qi, H., Kim, M., Ku, B., Azorin, D.D., Hausmann, D., Khajuria, R.K., Patel, A., and Maier, E. (2022). Patient-Derived Tumor Organoids for Guidance of Personalized Drug Therapies in Recurrent Glioblastoma. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23126572"},{"key":"ref_111","doi-asserted-by":"crossref","unstructured":"Morelli, M., Lessi, F., Franceschi, S., Ferri, G., Giacomarra, M., Menicagli, M., Gambacciani, C., Pieri, F., Pasqualetti, F., and Montemurro, N. (2024). Exploring Regorafenib Responsiveness and Uncovering Molecular Mechanisms in Recurrent Glioblastoma Tumors through Longitudinal In Vitro Sampling. Cells, 13.","DOI":"10.20944\/preprints202402.0501.v1"},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1186\/s13046-023-02843-6","article-title":"Albumin-bound paclitaxel augment temozolomide treatment sensitivity of glioblastoma cells by disrupting DNA damage repair and promoting ferroptosis","volume":"42","author":"Qu","year":"2023","journal-title":"J. Exp. Clin. Cancer Res."},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"6800","DOI":"10.1158\/1078-0432.CCR-21-0374","article-title":"A Modified Nucleoside 6-Thio-2\u2032-Deoxyguanosine Exhibits Antitumor Activity in Gliomas","volume":"27","author":"Yu","year":"2021","journal-title":"Clin. Cancer Res."},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1186\/s13059-020-01995-4","article-title":"CRISPRi-based radiation modifier screen identifies long non-coding RNA therapeutic targets in glioma","volume":"21","author":"Liu","year":"2020","journal-title":"Genome Biol."},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"1873","DOI":"10.1038\/s41556-023-01296-5","article-title":"Joint epigenome profiling reveals cell-type-specific gene regulatory programmes in human cortical organoids","volume":"25","author":"Noack","year":"2023","journal-title":"Nat. Cell Biol."},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"110846","DOI":"10.1016\/j.celrep.2022.110846","article-title":"Regionally defined proteomic profiles of human cerebral tissue and organoids reveal conserved molecular modules of neurodevelopment","volume":"39","author":"Melliou","year":"2022","journal-title":"Cell Rep."},{"key":"ref_117","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1038\/s41586-021-03209-8","article-title":"Single-cell epigenomics reveals mechanisms of human cortical development","volume":"598","author":"Ziffra","year":"2021","journal-title":"Nature"},{"key":"ref_118","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1038\/s41698-024-00500-5","article-title":"A multidimensional atlas of human glioblastoma-like organoids reveals highly coordinated molecular networks and effective drugs","volume":"8","author":"Wang","year":"2024","journal-title":"NPJ Precis. Oncol."},{"key":"ref_119","doi-asserted-by":"crossref","first-page":"e13558","DOI":"10.1111\/cpr.13558","article-title":"Integration of human organoids single-cell transcriptomic profiles and human genetics repurposes critical cell type-specific drug targets for severe COVID-19","volume":"57","author":"Ma","year":"2024","journal-title":"Cell Prolif."},{"key":"ref_120","doi-asserted-by":"crossref","first-page":"1635","DOI":"10.1016\/j.stemcr.2024.09.005","article-title":"Validation of non-destructive morphology-based selection of cerebral cortical organoids by paired morphological and single-cell RNA-seq analyses","volume":"19","author":"Ikeda","year":"2024","journal-title":"Stem Cell Rep."},{"key":"ref_121","doi-asserted-by":"crossref","first-page":"e111118","DOI":"10.15252\/embj.2022111118","article-title":"Gruffi: An algorithm for computational removal of stressed cells from brain organoid transcriptomic datasets","volume":"41","author":"Naas","year":"2022","journal-title":"Embo J."},{"key":"ref_122","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1186\/s13059-020-02257-z","article-title":"Evolutionary conservation and divergence of the human brain transcriptome","volume":"22","author":"Pembroke","year":"2021","journal-title":"Genome Biol."},{"key":"ref_123","doi-asserted-by":"crossref","first-page":"2382534","DOI":"10.1155\/2019\/2382534","article-title":"Genomics Analysis of Metabolic Pathways of Human Stem Cell-Derived Microglia-Like Cells and the Integrated Cortical Spheroids","volume":"2019","author":"Bejoy","year":"2019","journal-title":"Stem Cells Int."},{"key":"ref_124","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1038\/s41587-024-02198-z","article-title":"Glia-enriched cortical organoids implanted in mice capture astrocyte diversity","volume":"43","author":"Wang","year":"2025","journal-title":"Nat. Biotechnol."},{"key":"ref_125","doi-asserted-by":"crossref","first-page":"e2402287","DOI":"10.1002\/advs.202402287","article-title":"Single-Cell Transcriptome Landscape and Cell Fate Decoding in Human Brain Organoids after Transplantation","volume":"11","author":"Xu","year":"2024","journal-title":"Adv. Sci."},{"key":"ref_126","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1038\/s41586-022-05277-w","article-title":"Maturation and circuit integration of transplanted human cortical organoids","volume":"610","author":"Revah","year":"2022","journal-title":"Nature"},{"key":"ref_127","doi-asserted-by":"crossref","first-page":"682","DOI":"10.1111\/cns.13286","article-title":"Cerebral organoids transplantation improves neurological motor function in rat brain injury","volume":"26","author":"Wang","year":"2020","journal-title":"CNS Neurosci. Ther."},{"key":"ref_128","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1038\/s41536-023-00301-7","article-title":"Cerebral organoids transplantation repairs infarcted cortex and restores impaired function after stroke","volume":"8","author":"Cao","year":"2023","journal-title":"NPJ Regen. Med."},{"key":"ref_129","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.1093\/stcltm\/szae066","article-title":"Progranulin enhances the engraftment of transplanted human iPS cell-derived cerebral neurons","volume":"13","author":"Yamagami","year":"2024","journal-title":"Stem Cells Transl. Med."},{"key":"ref_130","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1016\/j.stemcr.2020.06.016","article-title":"Axonal Extensions along Corticospinal Tracts from Transplanted Human Cerebral Organoids","volume":"15","author":"Kitahara","year":"2020","journal-title":"Stem Cell Rep."},{"key":"ref_131","doi-asserted-by":"crossref","first-page":"e13201","DOI":"10.1111\/cpr.13201","article-title":"In vivo development and single-cell transcriptome profiling of human brain organoids","volume":"55","author":"Huang","year":"2022","journal-title":"Cell Prolif."},{"key":"ref_132","doi-asserted-by":"crossref","first-page":"9580","DOI":"10.1038\/s41467-024-53858-2","article-title":"Constructing organoid-brain-computer interfaces for neurofunctional repair after brain injury","volume":"15","author":"Hu","year":"2024","journal-title":"Nat. Commun."},{"key":"ref_133","doi-asserted-by":"crossref","first-page":"lsaa005","DOI":"10.1093\/jlb\/lsaa005","article-title":"Human cerebral organoids as a new legal and ethical challenge","volume":"7","author":"Lavazza","year":"2020","journal-title":"J. Law. Biosci."},{"key":"ref_134","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1136\/medethics-2017-104555","article-title":"Cerebral organoids: Ethical issues and consciousness assessment","volume":"44","author":"Lavazza","year":"2018","journal-title":"J. Med. Ethics"},{"key":"ref_135","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.tins.2019.11.003","article-title":"Are There Islands of Awareness?","volume":"43","author":"Bayne","year":"2020","journal-title":"Trends Neurosci."},{"key":"ref_136","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1111\/bioe.13290","article-title":"Weighing the moral status of brain organoids and research animals","volume":"38","author":"Koplin","year":"2024","journal-title":"Bioethics"},{"key":"ref_137","doi-asserted-by":"crossref","first-page":"1389","DOI":"10.1016\/j.stemcr.2023.05.019","article-title":"Donor perspectives on informed consent and use of biospecimens for brain organoid research","volume":"18","author":"MacDuffie","year":"2023","journal-title":"Stem Cell Rep."},{"key":"ref_138","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1016\/j.stemcr.2023.03.003","article-title":"Public perceptions and expectations: Disentangling the hope and hype of organoid research","volume":"18","author":"Ravn","year":"2023","journal-title":"Stem Cell Rep."},{"key":"ref_139","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/j.tibtech.2024.09.001","article-title":"Evaluating neuroprivacy concerns in human brain organoid research","volume":"43","author":"Kataoka","year":"2024","journal-title":"Trends Biotechnol."},{"key":"ref_140","doi-asserted-by":"crossref","first-page":"e13239","DOI":"10.1111\/cpr.13239","article-title":"Knowledge graphs of ethical concerns of cerebral organoids","volume":"55","author":"Ding","year":"2022","journal-title":"Cell Prolif."},{"key":"ref_141","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1186\/s13287-022-02950-9","article-title":"Organoids: A systematic review of ethical issues","volume":"13","author":"Massey","year":"2022","journal-title":"Stem Cell Res. Ther."}],"container-title":["Brain Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-3425\/16\/4\/427\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T04:24:16Z","timestamp":1777091056000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-3425\/16\/4\/427"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,19]]},"references-count":141,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2026,4]]}},"alternative-id":["brainsci16040427"],"URL":"https:\/\/doi.org\/10.3390\/brainsci16040427","relation":{},"ISSN":["2076-3425"],"issn-type":[{"value":"2076-3425","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,19]]}}}