{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,4]],"date-time":"2026-04-04T09:47:44Z","timestamp":1775296064878,"version":"3.50.1"},"reference-count":109,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2018,12,4]],"date-time":"2018-12-04T00:00:00Z","timestamp":1543881600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100016187","name":"Newlife Foundation for Disabled Children","doi-asserted-by":"publisher","award":["SG\/15-16\/11"],"award-info":[{"award-number":["SG\/15-16\/11"]}],"id":[{"id":"10.13039\/100016187","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Brain Sciences"],"abstract":"<jats:p>Unravelling the complex molecular pathways responsible for motor neuron degeneration in amyotrophic lateral sclerosis (ALS) and spinal muscular atrophy (SMA) remains a persistent challenge. Interest is growing in the potential molecular similarities between these two diseases, with the hope of better understanding disease pathology for the guidance of therapeutic development. The aim of this study was to conduct a comparative analysis of published proteomic studies of ALS and SMA, seeking commonly dysregulated molecules to be prioritized as future therapeutic targets. Fifteen proteins were found to be differentially expressed in two or more proteomic studies of both ALS and SMA, and bioinformatics analysis identified over-representation of proteins known to associate in vesicles and molecular pathways, including metabolism of proteins and vesicle-mediated transport\u2014both of which converge on endoplasmic reticulum (ER)-Golgi trafficking processes. Calreticulin, a calcium-binding chaperone found in the ER, was associated with both pathways and we independently confirm that its expression was decreased in spinal cords from SMA and increased in spinal cords from ALS mice. Together, these findings offer significant insights into potential common targets that may help to guide the development of new therapies for both diseases.<\/jats:p>","DOI":"10.3390\/brainsci8120212","type":"journal-article","created":{"date-parts":[[2018,12,4]],"date-time":"2018-12-04T11:56:18Z","timestamp":1543924578000},"page":"212","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Multi-Study Proteomic and Bioinformatic Identification of Molecular Overlap between Amyotrophic Lateral Sclerosis (ALS) and Spinal Muscular Atrophy (SMA)"],"prefix":"10.3390","volume":"8","author":[{"given":"Darija","family":"\u0160olti\u0107","sequence":"first","affiliation":[{"name":"School of Medicine, Keele University, Staffordshire ST5 5BG, UK"},{"name":"Institute for Science and Technology in Medicine, Keele University, Staffordshire ST5 5BG, UK"},{"name":"Wolfson Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, Oswestry SY10 7AG, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3579-6403","authenticated-orcid":false,"given":"Melissa","family":"Bowerman","sequence":"additional","affiliation":[{"name":"School of Medicine, Keele University, Staffordshire ST5 5BG, UK"},{"name":"Institute for Science and Technology in Medicine, Keele University, Staffordshire ST5 5BG, UK"},{"name":"Wolfson Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, Oswestry SY10 7AG, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joanne","family":"Stock","sequence":"additional","affiliation":[{"name":"School of Medicine, Keele University, Staffordshire ST5 5BG, UK"},{"name":"Institute for Science and Technology in Medicine, Keele University, Staffordshire ST5 5BG, UK"},{"name":"Wolfson Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, Oswestry SY10 7AG, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hannah K.","family":"Shorrock","sequence":"additional","affiliation":[{"name":"Biomedical Sciences, Edinburgh Medical School, University of Edinburgh, Edinburgh EH8 9AG, UK"},{"name":"Euan MacDonald Centre for Motor Neurone Disease Research, University of Edinburgh, Edinburgh EH8 9AG, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0306-5577","authenticated-orcid":false,"given":"Thomas H.","family":"Gillingwater","sequence":"additional","affiliation":[{"name":"Biomedical Sciences, Edinburgh Medical School, University of Edinburgh, Edinburgh EH8 9AG, UK"},{"name":"Euan MacDonald Centre for Motor Neurone Disease Research, University of Edinburgh, Edinburgh EH8 9AG, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heidi R.","family":"Fuller","sequence":"additional","affiliation":[{"name":"School of Medicine, Keele University, Staffordshire ST5 5BG, UK"},{"name":"Institute for Science and Technology in Medicine, Keele University, Staffordshire ST5 5BG, UK"},{"name":"Wolfson Centre for Inherited Neuromuscular Disease, RJAH Orthopaedic Hospital, Oswestry SY10 7AG, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2018,12,4]]},"reference":[{"key":"ref_1","first-page":"1","article-title":"Amyotrophic lateral sclerosis\u2014Looking for pathogenesis and effective therapy","volume":"49","author":"Naganska","year":"2011","journal-title":"Folia Neuropathol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1212\/01.wnl.0000296811.19811.db","article-title":"Genetics of familial amyotrophic lateral sclerosis","volume":"70","author":"Valdmanis","year":"2008","journal-title":"Neurology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1136\/jnnp-2016-315018","article-title":"Genetic epidemiology of amyotrophic lateral sclerosis: A systematic review and meta-analysis","volume":"88","author":"Zou","year":"2017","journal-title":"J. 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