{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,7]],"date-time":"2026-06-07T21:34:40Z","timestamp":1780868080822,"version":"3.54.1"},"reference-count":37,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2018,8,24]],"date-time":"2018-08-24T00:00:00Z","timestamp":1535068800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009395","name":"CancerCare Manitoba Foundation","doi-asserted-by":"publisher","award":["76 103 6238"],"award-info":[{"award-number":["76 103 6238"]}],"id":[{"id":"10.13039\/100009395","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>Classical Hodgkin\u2019s lymphoma (cHL) is a B-Cell lymphoma comprised of mononuclear Hodgkin cells (H) and bi- to multi-nucleated Reed-Sternberg (RS) cells. Previous studies revealed that H and RS cells express lamin A\/C, a component of the lamina of the nuclear matrix. Since no information was available about the three-dimensional (3D) expression patterns of lamin A\/C in H and RS cells, we analyzed the 3D spatial organization of lamin in such cells, using 3D fluorescent microscopy. H and RS cells from cHL derived cell lines stained positive for lamin A\/C, in contrast to peripheral blood lymphocytes (PBLs), in which the lamin A\/C protein was not detected or weak, although its presence could be transiently increased with lymphocyte activation by lipopolysaccharide (LPS). Most importantly, in H and RS cells, the regular homogeneous and spherically shaped lamin A\/C pattern, identified in activated lymphocytes, was absent. Instead, in H and RS cells, lamin staining showed internal lamin A\/C structures, subdividing the nuclei into two or more smaller compartments. Analysis of pre-treatment cHL patients\u2019 samples replicated the lamin patterns identified in cHL cell lines. We conclude that the investigation of lamin A\/C protein could be a useful tool for understanding nuclear remodeling in cHL.<\/jats:p>","DOI":"10.3390\/cancers10090286","type":"journal-article","created":{"date-parts":[[2018,8,24]],"date-time":"2018-08-24T11:13:45Z","timestamp":1535109225000},"page":"286","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Distinct 3D Structural Patterns of Lamin A\/C Expression in Hodgkin and Reed-Sternberg Cells"],"prefix":"10.3390","volume":"10","author":[{"given":"Fabio","family":"Contu","sequence":"first","affiliation":[{"name":"Cell Biology, Research Institute of Oncology and Hematology, University of Manitoba, CancerCare Manitoba, Winnipeg, MB R3E 0V9, Canada"},{"name":"Department of Biomedical Sciences, Unit of Biology and Genetics, University of Cagliari, 09042 Cagliari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aline","family":"Rangel-Pozzo","sequence":"additional","affiliation":[{"name":"Cell Biology, Research Institute of Oncology and Hematology, University of Manitoba, CancerCare Manitoba, Winnipeg, MB R3E 0V9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Trokajlo","sequence":"additional","affiliation":[{"name":"Cell Biology, Research Institute of Oncology and Hematology, University of Manitoba, CancerCare Manitoba, Winnipeg, MB R3E 0V9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Landon","family":"Wark","sequence":"additional","affiliation":[{"name":"Cell Biology, Research Institute of Oncology and Hematology, University of Manitoba, CancerCare Manitoba, Winnipeg, MB R3E 0V9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ludger","family":"Klewes","sequence":"additional","affiliation":[{"name":"Cell Biology, Research Institute of Oncology and Hematology, University of Manitoba, CancerCare Manitoba, Winnipeg, MB R3E 0V9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie A.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Division of Hematology and Division of Pathology, Department of Medicine, Jewish General Hospital, McGill University, Montr\u00e9al, QC H3G 2M1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tina","family":"Petrogiannis-Haliotis","sequence":"additional","affiliation":[{"name":"Division of Hematology and Division of Pathology, Department of Medicine, Jewish General Hospital, McGill University, Montr\u00e9al, QC H3G 2M1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John G.","family":"Gartner","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Manitoba, Winnipeg, MB R3E 0W2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuval","family":"Garini","sequence":"additional","affiliation":[{"name":"Physics Department &amp; Institute of Nanotechnology, Bar Ilan University, Ramat Gan 5290002, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1367-5958","authenticated-orcid":false,"given":"Roberta","family":"Vanni","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Unit of Biology and Genetics, University of Cagliari, 09042 Cagliari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7909-7866","authenticated-orcid":false,"given":"Hans","family":"Knecht","sequence":"additional","affiliation":[{"name":"Division of Hematology and Division of Pathology, Department of Medicine, Jewish General Hospital, McGill University, Montr\u00e9al, QC H3G 2M1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5797-2201","authenticated-orcid":false,"given":"Sabine","family":"Mai","sequence":"additional","affiliation":[{"name":"Cell Biology, Research Institute of Oncology and Hematology, University of Manitoba, CancerCare Manitoba, Winnipeg, MB R3E 0V9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2018,8,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2375","DOI":"10.1182\/blood-2016-01-643569","article-title":"The 2016 revision of the World Health Organization classification of lymphoid neoplasms","volume":"127","author":"Swerdlow","year":"2016","journal-title":"Blood"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"10962","DOI":"10.1073\/pnas.91.23.10962","article-title":"Hodgkin disease: Hodgkin and Reed-Sternberg cells picked from histological sections show clonal immunoglobulin gene rearrangements and appear to be derived from B cells at various stages of development","volume":"91","author":"Rajewsky","year":"1994","journal-title":"Proc. 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