{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T05:22:30Z","timestamp":1792905750958,"version":"4.5.0"},"reference-count":71,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2019,2,21]],"date-time":"2019-02-21T00:00:00Z","timestamp":1550707200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["NCI P30 CA036727"],"award-info":[{"award-number":["NCI P30 CA036727"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["NIH T32CA009476"],"award-info":[{"award-number":["NIH T32CA009476"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["F99CA212470"],"award-info":[{"award-number":["F99CA212470"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>FOXM1 is frequently overexpressed in cancer, but this has not been studied in a comprehensive manner. We utilized genotype-tissue expression (GTEx) normal and The Cancer Genome Atlas (TCGA) tumor data to define FOXM1 expression, including its isoforms, and to determine the genetic alterations that promote FOXM1 expression in cancer. Additionally, we used human fallopian tube epithelial (FTE) cells to dissect the role of Retinoblastoma (Rb)-E2F and Cyclin E1 in FOXM1 regulation, and a novel human embryonic kidney cell (HEK293T) CRISPR FOXM1 knockout model to define isoform-specific transcriptional programs. FOXM1 expression, at the mRNA and protein level, was significantly elevated in tumors with FOXM1 amplification, p53 inactivation, and Rb-E2F deregulation. FOXM1 expression was remarkably high in testicular germ cell tumors (TGCT), high-grade serous ovarian cancer (HGSC), and basal breast cancer (BBC). FOXM1 expression in cancer was associated with genomic instability, as measured using aneuploidy signatures. FTE models confirmed a role for Rb-E2F signaling in FOXM1 regulation and in particular identified Cyclin E1 as a novel inducer of FOXM1 expression. Among the three FOXM1 isoforms, FOXM1c showed the highest expression in normal and tumor tissues and cancer cell lines. The CRISPR knockout model demonstrated that FOXM1b and FOXM1c are transcriptionally active, while FOXM1a is not. Finally, we were unable to confirm the existence of a FOXM1 auto-regulatory loop. This study provides significant and novel information regarding the frequency, causes, and consequences of elevated FOXM1 expression in human cancer.<\/jats:p>","DOI":"10.3390\/cancers11020251","type":"journal-article","created":{"date-parts":[[2019,2,22]],"date-time":"2019-02-22T03:49:44Z","timestamp":1550807384000},"page":"251","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":220,"title":["Pan-Cancer Analyses Reveal Genomic Features of FOXM1 Overexpression in Cancer"],"prefix":"10.3390","volume":"11","author":[{"given":"Carter J","family":"Barger","sequence":"first","affiliation":[{"name":"Eppley Institute for Cancer Research, University of Nebraska Medical Center, Omaha, NE 68198, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Connor","family":"Branick","sequence":"additional","affiliation":[{"name":"Eppley Institute for Cancer Research, University of Nebraska Medical Center, Omaha, NE 68198, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda","family":"Chee","sequence":"additional","affiliation":[{"name":"Eppley Institute for Cancer Research, University of Nebraska Medical Center, Omaha, NE 68198, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0866-0666","authenticated-orcid":false,"given":"Adam R.","family":"Karpf","sequence":"additional","affiliation":[{"name":"Eppley Institute for Cancer Research, University of Nebraska Medical Center, Omaha, NE 68198, USA"},{"name":"Fred &amp; Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE 68198, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,2,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1626","DOI":"10.1128\/MCB.17.3.1626","article-title":"Hepatocyte nuclear factor 3\/fork head homolog 11 is expressed in proliferating epithelial and mesenchymal cells of embryonic and adult tissues","volume":"17","author":"Ye","year":"1997","journal-title":"Mol. Cell. Biol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1038\/ncb0205-108","article-title":"FoxM1 dances with mitosis","volume":"7","author":"Costa","year":"2005","journal-title":"Nat. Cell Biol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1038\/ncb1217","article-title":"FoxM1 is required for execution of the mitotic programme and chromosome stability","volume":"7","author":"Laoukili","year":"2005","journal-title":"Nat. Cell Biol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"5181","DOI":"10.1158\/0008-5472.CAN-04-4059","article-title":"Loss of the forkhead transcription factor FoxM1 causes centrosome amplification and mitotic catastrophe","volume":"65","author":"Wonsey","year":"2005","journal-title":"Cancer Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1038\/nature10166","article-title":"Integrated genomic analyses of ovarian carcinoma","volume":"474","year":"2011","journal-title":"Nature"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1038\/nature11412","article-title":"Comprehensive molecular portraits of human breast tumours","volume":"490","author":"Network","year":"2012","journal-title":"Nature"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"7731","DOI":"10.1073\/pnas.1424272112","article-title":"Inference of transcriptional regulation in cancers","volume":"112","author":"Jiang","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"34534","DOI":"10.18632\/oncotarget.15837","article-title":"Transcriptional landscape of human cancers","volume":"8","author":"Li","year":"2017","journal-title":"Oncotarget"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1016\/j.cell.2014.06.049","article-title":"Multiplatform analysis of 12 cancer types reveals molecular classification within and across tissues of origin","volume":"158","author":"Hoadley","year":"2014","journal-title":"Cell"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"938","DOI":"10.1038\/nm.3909","article-title":"The prognostic landscape of genes and infiltrating immune cells across human cancers","volume":"21","author":"Gentles","year":"2015","journal-title":"Nat. Med."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"5165","DOI":"10.1038\/ncomms6165","article-title":"Suppression of the FOXM1 transcriptional programme via novel small molecule inhibition","volume":"5","author":"Gormally","year":"2014","journal-title":"Nat. Commun."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1517\/14728222.2015.1042366","article-title":"Is there potential to target FOXM1 for \u2018undruggable\u2019 lung cancers?","volume":"19","author":"Kalinichenko","year":"2015","journal-title":"Expert Opin. Ther. Targets"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Bhat, U.G., Halasi, M., and Gartel, A.L. (2009). FoxM1 is a general target for proteasome inhibitors. PLoS ONE, 4.","DOI":"10.1371\/journal.pone.0006593"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1517\/14728222.12.6.663","article-title":"FoxM1 inhibitors as potential anticancer drugs","volume":"12","author":"Gartel","year":"2008","journal-title":"Expert Opin. Ther. Targets"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"4341","DOI":"10.4161\/cc.10.24.18544","article-title":"Thiostrepton, proteasome inhibitors and FOXM1","volume":"10","author":"Gartel","year":"2011","journal-title":"Cell Cycle"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1715","DOI":"10.1093\/nar\/25.9.1715","article-title":"The winged-helix transcription factor Trident is expressed in cycling cells","volume":"25","author":"Korver","year":"1997","journal-title":"Nucleic Acids Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1038\/onc.2016.249","article-title":"A novel FOXM1 isoform, FOXM1D, promotes epithelial-mesenchymal transition and metastasis through ROCKs activation in colorectal cancer","volume":"36","author":"Zhang","year":"2017","journal-title":"Oncogene"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"E3050","DOI":"10.1073\/pnas.1508057112","article-title":"Systematic transcriptome analysis reveals tumor-specific isoforms for ovarian cancer diagnosis and therapy","volume":"112","author":"Barrett","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"4917","DOI":"10.1128\/MCB.01789-06","article-title":"Induction of Mxi1-SR alpha by FOXO3a contributes to repression of Myc-dependent gene expression","volume":"27","author":"Delpuech","year":"2007","journal-title":"Mol. Cell. Biol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1477","DOI":"10.1158\/1078-0432.CCR-13-2311","article-title":"FOXM1c promotes pancreatic cancer epithelial-to-mesenchymal transition and metastasis via upregulation of expression of the urokinase plasminogen activator system","volume":"20","author":"Huang","year":"2014","journal-title":"Clin. Cancer Res."},{"key":"ref_21","first-page":"11","article-title":"FOXM1b, which is present at elevated levels in cancer cells, has a greater transforming potential than FOXM1c","volume":"3","author":"Lam","year":"2013","journal-title":"Front. Oncol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3593","DOI":"10.1158\/0008-5472.CAN-05-2912","article-title":"FoxM1B is overexpressed in human glioblastomas and critically regulates the tumorigenicity of glioma cells","volume":"66","author":"Liu","year":"2006","journal-title":"Cancer Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1007\/978-1-60761-738-9_6","article-title":"Raf\/MEK\/MAPK signaling stimulates the nuclear translocation and transactivating activity of FOXM1","volume":"647","author":"Ma","year":"2010","journal-title":"Methods Mol. Biol."},{"key":"ref_24","first-page":"4773","article-title":"FOXM1 is a downstream target of Gli1 in basal cell carcinomas","volume":"62","author":"Teh","year":"2002","journal-title":"Cancer Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"27613","DOI":"10.18632\/oncotarget.4546","article-title":"Genetic determinants of FOXM1 overexpression in epithelial ovarian cancer and functional contribution to cell cycle progression","volume":"6","author":"Barger","year":"2015","journal-title":"Oncotarget"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"3987","DOI":"10.1158\/0008-5472.CAN-12-3859","article-title":"Dysregulated expression of FOXM1 isoforms drives progression of pancreatic cancer","volume":"73","author":"Kong","year":"2013","journal-title":"Cancer Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1038\/nbt.3772","article-title":"Toil enables reproducible, open source, big biomedical data analyses","volume":"35","author":"Vivian","year":"2017","journal-title":"Nat. Biotechnol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1186\/s13046-017-0536-y","article-title":"FOXM1 expression is significantly associated with chemotherapy resistance and adverse prognosis in non-serous epithelial ovarian cancer patients","volume":"36","author":"Tassi","year":"2017","journal-title":"J. Exp. Clin. Cancer Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1242\/jcs.01657","article-title":"Raf\/MEK\/MAPK signaling stimulates the nuclear translocation and transactivating activity of FOXM1c","volume":"118","author":"Ma","year":"2005","journal-title":"J. Cell Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1515\/BC.2006.120","article-title":"Despite its strong transactivation domain, transcription factor FOXM1c is kept almost inactive by two different inhibitory domains","volume":"387","author":"Wierstra","year":"2006","journal-title":"Biol. Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1158\/1535-7163.MCT-12-0712","article-title":"FOX(M1) news\u2014It is cancer","volume":"12","author":"Halasi","year":"2013","journal-title":"Mol. Cancer Ther."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Delahaye-Sourdeix, M., Oliver, J., Timofeeva, M.N., Gaborieau, V., Johansson, M., Chabrier, A., Wozniak, M.B., Brenner, D.R., Vallee, M.P., and Anantharaman, D. (2015). The 12p13.33\/RAD52 Locus and Genetic Susceptibility to Squamous Cell Cancers of Upper Aerodigestive Tract. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0117639"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1002\/gcc.20856","article-title":"Integrative genomic profiling reveals conserved genetic mechanisms for tumorigenesis in common entities of non-Hodgkin\u2019s lymphoma","volume":"50","author":"Green","year":"2011","journal-title":"Genes Chromosomes Cancer"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1924","DOI":"10.1158\/1078-0432.CCR-10-1551","article-title":"Array-based comparative genomic hybridization identifies CDK4 and FOXM1 alterations as independent predictors of survival in malignant peripheral nerve sheath tumor","volume":"17","author":"Yu","year":"2011","journal-title":"Clin. Cancer Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1038\/nature11003","article-title":"The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity","volume":"483","author":"Barretina","year":"2012","journal-title":"Nature"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1101\/gr.212225.116","article-title":"Pan-cancer analysis distinguishes transcriptional changes of aneuploidy from proliferation","volume":"27","author":"Buccitelli","year":"2017","journal-title":"Genome Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1038\/ng1861","article-title":"A signature of chromosomal instability inferred from gene expression profiles predicts clinical outcome in multiple human cancers","volume":"38","author":"Carter","year":"2006","journal-title":"Nat. Genet."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/j.cell.2018.03.022","article-title":"Cell-of-Origin Patterns Dominate the Molecular Classification of 10,000 Tumors from 33 Types of Cancer","volume":"173","author":"Hoadley","year":"2018","journal-title":"Cell"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1016\/j.ccell.2018.03.007","article-title":"Genomic and Functional Approaches to Understanding Cancer Aneuploidy","volume":"33","author":"Taylor","year":"2018","journal-title":"Cancer Cell"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1002\/gcc.10244","article-title":"High-resolution comparative genomic hybridization detects extra chromosome arm 12p material in most cases of carcinoma in situ adjacent to overt germ cell tumors, but not before the invasive tumor development","volume":"38","author":"Ottesen","year":"2003","journal-title":"Genes Chromosomes Cancer"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"3392","DOI":"10.1016\/j.celrep.2018.05.039","article-title":"Integrated Molecular Characterization of Testicular Germ Cell Tumors","volume":"23","author":"Shen","year":"2018","journal-title":"Cell Rep."},{"key":"ref_42","unstructured":"Barger, C.J., Branick, C., Chee, L., and Karpf, A.R. Personal communication."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2758","DOI":"10.1016\/j.celrep.2018.04.102","article-title":"Identification of Drivers of Aneuploidy in Breast Tumors","volume":"23","author":"Pfister","year":"2018","journal-title":"Cell Rep."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"3425","DOI":"10.4161\/cc.8.20.9628","article-title":"p53 negatively regulates expression of FoxM1","volume":"8","author":"Pandit","year":"2009","journal-title":"Cell Cycle"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1046","DOI":"10.1158\/1535-7163.MCT-11-0024","article-title":"ATM and p53 regulate FOXM1 expression via E2F in breast cancer epirubicin treatment and resistance","volume":"10","author":"Millour","year":"2011","journal-title":"Mol. Cancer Ther."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"4295","DOI":"10.1038\/onc.2009.282","article-title":"Pro-proliferative FoxM1 is a target of p53-mediated repression","volume":"28","author":"Barsotti","year":"2009","journal-title":"Oncogene"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1134","DOI":"10.1038\/ng.2760","article-title":"Pan-cancer patterns of somatic copy number alteration","volume":"45","author":"Zack","year":"2013","journal-title":"Nat. Genet."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1755","DOI":"10.1038\/nprot.2012.097","article-title":"Primary culture and immortalization of human fallopian tube secretory epithelial cells","volume":"7","author":"Karst","year":"2012","journal-title":"Nat. Protoc."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"7547","DOI":"10.1073\/pnas.1017300108","article-title":"Modeling high-grade serous ovarian carcinogenesis from the fallopian tube","volume":"108","author":"Karst","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1875","DOI":"10.1101\/gad.264127.115","article-title":"Proteomic analysis of pRb loss highlights a signature of decreased mitochondrial oxidative phosphorylation","volume":"29","author":"Nicolay","year":"2015","journal-title":"Genes Dev."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1038\/nmeth.3047","article-title":"Improved vectors and genome-wide libraries for CRISPR screening","volume":"11","author":"Sanjana","year":"2014","journal-title":"Nat. Methods"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1158\/0008-5472.CAN-13-2247","article-title":"Cyclin E1 deregulation occurs early in secretory cell transformation to promote formation of fallopian tube-derived high-grade serous ovarian cancers","volume":"74","author":"Karst","year":"2014","journal-title":"Cancer Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1006\/geno.1997.5065","article-title":"The human TRIDENT\/HFH-11\/FKHL16 gene: Structure, localization, and promoter characterization","volume":"46","author":"Korver","year":"1997","journal-title":"Genomics"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/S0171-2985(97)80036-8","article-title":"The winged-helix transcription factor Trident is expressed in actively dividing lymphocytes","volume":"198","author":"Korver","year":"1997","journal-title":"Immunobiology"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1966","DOI":"10.4161\/cc.8.12.8708","article-title":"A novel mode of FoxM1 regulation: Positive auto-regulatory loop","volume":"8","author":"Halasi","year":"2009","journal-title":"Cell Cycle"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Cheng, X.H., Black, M., Ustiyan, V., Le, T., Fulford, L., Sridharan, A., Medvedovic, M., Kalinichenko, V.V., Whitsett, J.A., and Kalin, T.V. (2014). SPDEF inhibits prostate carcinogenesis by disrupting a positive feedback loop in regulation of the Foxm1 oncogene. PLoS Genet., 10.","DOI":"10.1371\/journal.pgen.1004656"},{"key":"ref_57","first-page":"87","article-title":"Identification of FOXM1 as a specific marker for triple-negative breast cancer","volume":"54","author":"Tan","year":"2019","journal-title":"Int. J. Oncol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"2393","DOI":"10.1101\/gad.813200","article-title":"The Rb\/E2F pathway: Expanding roles and emerging paradigms","volume":"14","author":"Harbour","year":"2000","journal-title":"Genes Dev."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"4424","DOI":"10.1038\/onc.2013.394","article-title":"FOXO3a loss is a frequent early event in high-grade pelvic serous carcinogenesis","volume":"33","author":"Levanon","year":"2014","journal-title":"Oncogene"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1002\/path.4649","article-title":"In vitro and in vivo correlates of physiological and neoplastic human Fallopian tube stem cells","volume":"238","author":"Yamamoto","year":"2016","journal-title":"J. Pathol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"956","DOI":"10.1038\/onc.2016.262","article-title":"FOXM1 allows human keratinocytes to bypass the oncogene-induced differentiation checkpoint in response to gain of MYC or loss of p53","volume":"36","author":"Molinuevo","year":"2017","journal-title":"Oncogene"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1016\/j.molcel.2014.02.001","article-title":"Uncovering SUMOylation dynamics during cell-cycle progression reveals FoxM1 as a key mitotic SUMO target protein","volume":"53","author":"Schimmel","year":"2014","journal-title":"Mol. Cell"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"3076","DOI":"10.1128\/MCB.01710-07","article-title":"Activation of FoxM1 during G2 requires cyclin A\/Cdk-dependent relief of autorepression by the FoxM1 N-terminal domain","volume":"28","author":"Laoukili","year":"2008","journal-title":"Mol. Cell. Biol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1186\/s13059-015-0696-z","article-title":"FOXM1 binds directly to non-consensus sequences in the human genome","volume":"16","author":"Sanders","year":"2015","journal-title":"Genome Biol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"pl1","DOI":"10.1126\/scisignal.2004088","article-title":"Integrative analysis of complex cancer genomics and clinical profiles using the cBioPortal","volume":"6","author":"Gao","year":"2013","journal-title":"Sci. Signal."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1046","DOI":"10.1038\/nmeth.2650","article-title":"TCPA: A resource for cancer functional proteomics data","volume":"10","author":"Li","year":"2013","journal-title":"Nat. Methods"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"e51","DOI":"10.1158\/0008-5472.CAN-17-0369","article-title":"Explore, Visualize, and Analyze Functional Cancer Proteomic Data Using the Cancer Proteome Atlas","volume":"77","author":"Li","year":"2017","journal-title":"Cancer Res."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"3887","DOI":"10.1038\/ncomms4887","article-title":"A pan-cancer proteomic perspective on The Cancer Genome Atlas","volume":"5","author":"Akbani","year":"2014","journal-title":"Nat. Commun."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1126","DOI":"10.1038\/s41467-017-01153-8","article-title":"Gene isoforms as expression-based biomarkers predictive of drug response in vitro","volume":"8","author":"Safikhani","year":"2017","journal-title":"Nat. Commun."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1008","DOI":"10.4161\/cbt.9.12.11710","article-title":"Negative regulation of the oncogenic transcription factor FoxM1 by thiazolidinediones and mithramycin","volume":"9","author":"Petrovic","year":"2010","journal-title":"Cancer Biol. Ther."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"2281","DOI":"10.1038\/nprot.2013.143","article-title":"Genome engineering using the CRISPR-Cas9 system","volume":"8","author":"Ran","year":"2013","journal-title":"Nat. Protoc."}],"container-title":["Cancers"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6694\/11\/2\/251\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T12:33:56Z","timestamp":1760186036000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6694\/11\/2\/251"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,2,21]]},"references-count":71,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2019,2]]}},"alternative-id":["cancers11020251"],"URL":"https:\/\/doi.org\/10.3390\/cancers11020251","relation":{},"ISSN":["2072-6694"],"issn-type":[{"value":"2072-6694","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,2,21]]}}}