{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:37:12Z","timestamp":1787027832795,"version":"build-2736575974"},"reference-count":53,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2019,6,20]],"date-time":"2019-06-20T00:00:00Z","timestamp":1560988800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Familie Mehdorn Stiftung","award":["Q-026. 0089"],"award-info":[{"award-number":["Q-026. 0089"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>Gap junctions have recently been shown to interconnect glioblastoma cells to a multicellular syncytial network, thereby allowing intercellular communication over long distances as well as enabling glioblastoma cells to form routes for brain microinvasion. Against this backdrop gap junction-targeted therapies might provide for an essential contribution to isolate cancer cells within the brain, thus increasing the tumor cells\u2019 vulnerability to the standard chemotherapeutic agent temozolomide. By utilizing INI-0602\u2014a novel gap junction inhibitor optimized for crossing the blood brain barrier\u2014in an oncological setting, the present study was aimed at evaluating the potential of gap junction-targeted therapy on primary human glioblastoma cell populations. Pharmacological inhibition of gap junctions profoundly sensitized primary glioblastoma cells to temozolomide-mediated cell death. On the molecular level, gap junction inhibition was associated with elevated activity of the JNK signaling pathway. With the use of a novel gap junction inhibitor capable of crossing the blood\u2013brain barrier\u2014thus constituting an auspicious drug for clinical applicability\u2014these results may constitute a promising new therapeutic strategy in the field of current translational glioblastoma research.<\/jats:p>","DOI":"10.3390\/cancers11060858","type":"journal-article","created":{"date-parts":[[2019,6,20]],"date-time":"2019-06-20T10:49:59Z","timestamp":1561027799000},"page":"858","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Inhibition of Gap Junctions Sensitizes Primary Glioblastoma Cells for Temozolomide"],"prefix":"10.3390","volume":"11","author":[{"given":"Anna-Laura","family":"Potthoff","sequence":"first","affiliation":[{"name":"Department of Neurosurgery, Rheinische Friedrich-Wilhelms-University Hospital, Sigmund-Freud-Strasse 25, 53127 Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9258-3033","authenticated-orcid":false,"given":"Dieter Henrik","family":"Heiland","sequence":"additional","affiliation":[{"name":"Translational NeuroOncology Research Group, University of Freiburg, Breisacher Strasse 64, 79106 Freiburg im Breisgau, Germany"},{"name":"Faculty of Medicine, University of Freiburg, Breisacher Strasse 64, 79106 Freiburg im Breisgau, Germany"},{"name":"Department of Neurosurgery, University of Freiburg, Breisacher Strasse 64, 79106 Freiburg im Breisgau, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernd O.","family":"Evert","sequence":"additional","affiliation":[{"name":"Department of Neurology, Rheinische Friedrich-Wilhelms-University Hospital, Sigmund-Freud-Strasse 25, 53127 Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filipe Rodrigues","family":"Almeida","sequence":"additional","affiliation":[{"name":"Department of Neuropathology, Rheinische Friedrich-Wilhelms-University Hospital, Sigmund-Freud-Strasse 25, 53127 Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon P.","family":"Behringer","sequence":"additional","affiliation":[{"name":"Translational NeuroOncology Research Group, University of Freiburg, Breisacher Strasse 64, 79106 Freiburg im Breisgau, Germany"},{"name":"Faculty of Medicine, University of Freiburg, Breisacher Strasse 64, 79106 Freiburg im Breisgau, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Dolf","sequence":"additional","affiliation":[{"name":"Institute of Experimental Immunology, Rheinische Friedrich-Wilhelms-University Hospital, Sigmund-Freud-Strasse 25, 53127 Bonn, 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