{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T12:36:12Z","timestamp":1769603772550,"version":"3.49.0"},"reference-count":43,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2019,8,22]],"date-time":"2019-08-22T00:00:00Z","timestamp":1566432000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["P 25599"],"award-info":[{"award-number":["P 25599"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["P25925"],"award-info":[{"award-number":["P25925"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["P26908"],"award-info":[{"award-number":["P26908"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["P29222"],"award-info":[{"award-number":["P29222"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>Genetically-engineered mouse models (GEMMs) lacking diseased-associated gene(s) globally or in a tissue-specific manner represent an attractive tool with which to assess the efficacy and toxicity of targeted pharmacological inhibitors. Stat3 and Stat5a\/b transcription factors have been implicated in several pathophysiological conditions, and pharmacological inhibition of both transcription factors has been proposed to treat certain diseases, such as malignancies. To model combined inhibition of Stat3 and Stat5a\/b we have developed a GEMM harboring a flox Stat3-Stat5a\/b allele (Stat5\/3loxP\/loxP mice) and generated mice lacking Stat3 and Stat5a\/b in hepatocytes (Stat5\/3\u0394hep\/\u0394hep). Stat5\/3\u0394hep\/\u0394hep mice exhibited a marked reduction of STAT3, STAT5A and STAT5B proteins in the liver and developed steatosis, a phenotype that resembles mice lacking Stat5a\/b in hepatocytes. In addition, embryonic deletion of Stat3 and Stat5a\/b (Stat5\/3\u0394\/\u0394 mice) resulted in lethality, similar to Stat3\u0394\/\u0394 mice. This data illustrates that Stat5\/3loxP\/loxP mice are functional and can be used as a valuable tool to model the combined inhibition of Stat3 and Stat5a\/b in tumorigenesis and other diseases.<\/jats:p>","DOI":"10.3390\/cancers11091226","type":"journal-article","created":{"date-parts":[[2019,8,23]],"date-time":"2019-08-23T10:15:07Z","timestamp":1566555307000},"page":"1226","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A Mouse Model to Assess STAT3 and STAT5A\/B Combined Inhibition in Health and Disease Conditions"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6438-9068","authenticated-orcid":false,"given":"Herwig P.","family":"Moll","sequence":"first","affiliation":[{"name":"Department of Physiology, Center of Physiology and Pharmacology, Comprehensive Cancer Center (CCC), Medical University of Vienna, 1090 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julian","family":"Mohrherr","sequence":"additional","affiliation":[{"name":"Ludwig Boltzmann Institute for Cancer Research (LBI-CR), 1090 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leander","family":"Blaas","sequence":"additional","affiliation":[{"name":"Ludwig Boltzmann Institute for Cancer Research (LBI-CR), 1090 Vienna, Austria"},{"name":"Department of Biosciences and Nutrition, Center for Innovative Medicine, Karolinska Institutet, Novum, 14183 Huddinge, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monica","family":"Musteanu","sequence":"additional","affiliation":[{"name":"Ludwig Boltzmann Institute for Cancer Research (LBI-CR), 1090 Vienna, Austria"},{"name":"CNIO (Spanish National Cancer Research Centre), E-28029 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia","family":"Stiedl","sequence":"additional","affiliation":[{"name":"Ludwig Boltzmann Institute for Cancer Research (LBI-CR), 1090 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatrice","family":"Grabner","sequence":"additional","affiliation":[{"name":"Ludwig Boltzmann Institute for Cancer Research (LBI-CR), 1090 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katalin","family":"Zboray","sequence":"additional","affiliation":[{"name":"Ludwig Boltzmann Institute for Cancer Research (LBI-CR), 1090 Vienna, Austria"},{"name":"Plant Protection Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, 2462 Martonv\u00e1s\u00e1r, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margit","family":"K\u00f6nig","sequence":"additional","affiliation":[{"name":"Children\u2019s Cancer Research Institute, St. Anna Kinderkrebsforschung, 1090 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8824-0767","authenticated-orcid":false,"given":"Dagmar","family":"Stoiber","sequence":"additional","affiliation":[{"name":"Ludwig Boltzmann Institute for Cancer Research (LBI-CR), 1090 Vienna, Austria"},{"name":"Department of Pharmacology, Center of Physiology and Pharmacology, Comprehensive Cancer Center (CCC), Medical University of Vienna, 1090 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"R\u00fclicke","sequence":"additional","affiliation":[{"name":"Institute of Laboratory Animal, Science, University of Veterinary Medicine Vienna, 1210 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0179-0628","authenticated-orcid":false,"given":"Sabine","family":"Strehl","sequence":"additional","affiliation":[{"name":"Children\u2019s Cancer Research Institute, St. Anna Kinderkrebsforschung, 1090 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Eferl","sequence":"additional","affiliation":[{"name":"Institute of Cancer Research, Medical University Vienna &amp; Comprehensive Cancer Center (CCC), 1090 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7992-5361","authenticated-orcid":false,"given":"Emilio","family":"Casanova","sequence":"additional","affiliation":[{"name":"Department of Physiology, Center of Physiology and Pharmacology, Comprehensive Cancer Center (CCC), Medical University of Vienna, 1090 Vienna, Austria"},{"name":"Ludwig Boltzmann Institute for Cancer Research (LBI-CR), 1090 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,8,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"267","DOI":"10.4062\/biomolther.2014.074","article-title":"Genetically engineered mouse models for drug development and preclinical trials","volume":"22","author":"Lee","year":"2014","journal-title":"Biomol. 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