{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T18:16:07Z","timestamp":1776795367240,"version":"3.51.2"},"reference-count":72,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2019,10,17]],"date-time":"2019-10-17T00:00:00Z","timestamp":1571270400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003852","name":"REGIONE CAMPANIA","doi-asserted-by":"publisher","award":["PG\/2017\/0623667"],"award-info":[{"award-number":["PG\/2017\/0623667"]}],"id":[{"id":"10.13039\/501100003852","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>Oral (OSCC) and oropharyngeal (OPSCC) squamous cell carcinomas show high morbidity and mortality rates. We aimed to investigate the role of the \u201cChromatin Assembly Factor-1\u201d (CAF-1) p60 and p150 subunits, involved in DNA repair and replication, in OSCC and OPSCC progression and in response to Poly(ADP-ribose) polymerase (PARP)-inhibitors and exposure to ionizing radiation (IR). We immunostained tissue microarrays (TMAs), including 112 OSCC and 42 OPSCC, with anti-CAF-1\/p60 and anti-CAF-1\/p150 specific antibodies, correlating their expression with prognosis. Moreover, we assessed the sensitivity to PARP inhibitors and the double-strand breaks repair proficiency by cell viability and HR reporter assays, respectively, in HPV-positive and HPV-negative cell lines upon CAF-1\/p60 and CAF-1\/p150 depletion. The immunohistochemical analysis revealed a significant prognostic value of both tissue biomarkers combined expression in OSCC but not in OPSCC. In in vitro studies, the p60\/150 CAF-1 subunits\u2019 depletion impaired the proficiency of Homologous Recombination DNA damage repair, inducing sensitivity to the PARP-inhibitors, able to sensitize both the cell lines to IR. These results indicate that regardless of the prognostic meaning of p60\/p150 tissue expression, the pharmacological depletion of CAF-1 complex\u2019s function, combined to PARP-inhibitors and\/or IR treatment, could represent a valid therapeutic strategy for squamous cell carcinomas of head and neck region.<\/jats:p>","DOI":"10.3390\/cancers11101582","type":"journal-article","created":{"date-parts":[[2019,10,17]],"date-time":"2019-10-17T11:07:59Z","timestamp":1571310479000},"page":"1582","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["CAF-1 Subunits Levels Suggest Combined Treatments with PARP-Inhibitors and Ionizing Radiation in Advanced HNSCC"],"prefix":"10.3390","volume":"11","author":[{"given":"Francesco","family":"Morra","sequence":"first","affiliation":[{"name":"Institute for the Experimental Endocrinology and Oncology, National Research Council, 80131 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0711-6738","authenticated-orcid":false,"given":"Francesco","family":"Merolla","sequence":"additional","affiliation":[{"name":"Department of Advanced Biomedical Sciences, University \u201cFederico II\u201d, Surgical Pathology Section, 80131 Naples, Italy"},{"name":"Department of Medicine and Health Sciences \u201cV. Tiberio\u201d, University of Molise, 86100 Campobasso, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ida","family":"Picardi","sequence":"additional","affiliation":[{"name":"Institute for the Experimental Endocrinology and Oncology, National Research Council, 80131 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela","family":"Russo","sequence":"additional","affiliation":[{"name":"Department of Advanced Biomedical Sciences, University \u201cFederico II\u201d, Surgical Pathology Section, 80131 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9354-4087","authenticated-orcid":false,"given":"Gennaro","family":"Ilardi","sequence":"additional","affiliation":[{"name":"Department of Advanced Biomedical Sciences, University \u201cFederico II\u201d, Surgical Pathology Section, 80131 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Varricchio","sequence":"additional","affiliation":[{"name":"Department of Advanced Biomedical Sciences, University \u201cFederico II\u201d, Surgical Pathology Section, 80131 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Federica","family":"Liotti","sequence":"additional","affiliation":[{"name":"Institute for the Experimental Endocrinology and Oncology, National Research Council, 80131 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto","family":"Pacelli","sequence":"additional","affiliation":[{"name":"Department of Advanced Biomedical Sciences, University \u201cFederico II\u201d, Surgical Pathology Section, 80131 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5556-5549","authenticated-orcid":false,"given":"Luca","family":"Palazzo","sequence":"additional","affiliation":[{"name":"Institute for the Experimental Endocrinology and Oncology, National Research Council, 80131 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2576-220X","authenticated-orcid":false,"given":"Massimo","family":"Mascolo","sequence":"additional","affiliation":[{"name":"Department of Advanced Biomedical Sciences, University \u201cFederico II\u201d, Surgical Pathology Section, 80131 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5166-3507","authenticated-orcid":false,"given":"Angela","family":"Celetti","sequence":"additional","affiliation":[{"name":"Institute for the Experimental Endocrinology and Oncology, National Research Council, 80131 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefania","family":"Staibano","sequence":"additional","affiliation":[{"name":"Department of Advanced Biomedical Sciences, University \u201cFederico II\u201d, Surgical Pathology Section, 80131 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2019,10,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"7","DOI":"10.3322\/caac.21551","article-title":"Cancer statistics, 2019","volume":"69","author":"Siegel","year":"2019","journal-title":"CA Cancer J. 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