{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T02:43:11Z","timestamp":1760236991878,"version":"build-2065373602"},"reference-count":24,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2020,2,4]],"date-time":"2020-02-04T00:00:00Z","timestamp":1580774400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>Background: Rathke\u2019s Cleft Cysts (RCCs) are rare epithelial cysts arising from remnants of the Rathke pouch in the pituitary gland. A subset of these lesions enlarge and produce a mass effect with consequent hypopituitarism, and may result in visual loss. Moreover, some RCCs with a high intra-cystic protein content may mimic cystic pituitary adenoma, which makes their differential diagnosis ambiguous. Currently, medical professionals have no definitive way to distinguish RCCs from pituitary adenomas. Therefore, preoperative confirmation of RCCs would be of help to medical professionals for the management and proper surgical decision making. The goal of this study is to identify molecular markers in RCCs. Methods: We characterized aqueous and chloroform extracts of surgically resected RCCs and pituitary adenomas using ex vivo 1H NMR spectroscopy. Results: All RCCs exclusively showed the presence of mucopolysaccharides which are glycosaminoglycans (GAGs) made up of disaccharides of aminosugars and uronic sugars. Conclusion: GAGs can be used as metabolite marker for the detection of RCCs and this knowledge will lay the groundwork for the development of a non-invasive, in vivo magnetic resonance spectroscopy methodology for the differential diagnosis of RCCs and pituitary adenomas using clinical MRI scanners.<\/jats:p>","DOI":"10.3390\/cancers12020360","type":"journal-article","created":{"date-parts":[[2020,2,6]],"date-time":"2020-02-06T02:59:18Z","timestamp":1580957958000},"page":"360","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Proton Magnetic Resonance Spectroscopy Characterization of Rathke\u2019s Cleft Cysts (RCCs): Relevance to the Differential Diagnosis of Pituitary Adenomas and RCCs"],"prefix":"10.3390","volume":"12","author":[{"given":"Omkar B.","family":"Ijare","sequence":"first","affiliation":[{"name":"Kenneth R. Peak Brain and Pituitary Tumor Treatment Center, Department of Neurosurgery, Houston Methodist Neurological Institute, Houston Methodist Hospital and Research Institute, Houston, TX 77030, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martyn A.","family":"Sharpe","sequence":"additional","affiliation":[{"name":"Kenneth R. Peak Brain and Pituitary Tumor Treatment Center, Department of Neurosurgery, Houston Methodist Neurological Institute, Houston Methodist Hospital and Research Institute, Houston, TX 77030, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David S.","family":"Baskin","sequence":"additional","affiliation":[{"name":"Kenneth R. Peak Brain and Pituitary Tumor Treatment Center, Department of Neurosurgery, Houston Methodist Neurological Institute, Houston Methodist Hospital and Research Institute, Houston, TX 77030, USA"},{"name":"Department of Neurological Surgery, Weill Cornell Medical College, New York, NY 10065, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kumar","family":"Pichumani","sequence":"additional","affiliation":[{"name":"Kenneth R. Peak Brain and Pituitary Tumor Treatment Center, Department of Neurosurgery, Houston Methodist Neurological Institute, Houston Methodist Hospital and Research Institute, Houston, TX 77030, USA"},{"name":"Department of Neurological Surgery, Weill Cornell Medical College, New York, NY 10065, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,2,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/B978-0-444-59602-4.00017-4","article-title":"Rathke\u2019s cleft cyst","volume":"124","author":"Larkin","year":"2014","journal-title":"Handb. Clin. Neurol."},{"unstructured":"Feingold, K.R., Anawalt, B., Boyce, A., Chrousos, G., Dungan, K., Grossman, A., Hershman, J.M., Kaltsas, G., Koch, C., and Kopp, P. (2000). Pathology and Pathogenesis of Pituitary Adenomas and Other Sellar Lesions, MDText.com, Inc.. Endotext [Internet].","key":"ref_2"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1227\/00006123-199202000-00004","article-title":"Radiologic characteristics and results of surgical management of Rathke\u2019s cysts in 43 patients","volume":"30","author":"Ross","year":"1992","journal-title":"Neurosurgery"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"E3","DOI":"10.3171\/2011.5.FOCUS1178","article-title":"Rathke cleft cysts in pediatric patients: presentation, surgical management, and postoperative outcomes","volume":"3","author":"Jahangiri","year":"2011","journal-title":"Neurosurg Focus."},{"key":"ref_5","first-page":"485","article-title":"MR Imaging Findings of Rathke\u2019s Cleft Cysts: Significance of Intracystic Nodules","volume":"21","author":"Byun","year":"2000","journal-title":"Am. J. Neuroradiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.ijsu.2015.05.001","article-title":"Diagnosis of concomitant pituitary adenoma and Rathke\u2019s cleft cyst with magnetic resonance imaging","volume":"18","author":"Guo","year":"2015","journal-title":"Int. J. Surg."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1097\/00004728-199901000-00008","article-title":"Rathke cleft cyst: MR and biomedical analysis of cyst content","volume":"23","author":"Hayashi","year":"1999","journal-title":"J. Comput Assist Tomogr."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"183","DOI":"10.4103\/1793-5482.125662","article-title":"Symptomatic Rathke\u2019s cleft cyst with a co-existing pituitary tumor; Brief review of the literature","volume":"8","author":"Babu","year":"2013","journal-title":"Asian J. Neurosurg."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1007\/s00701-007-1234-x","article-title":"Rathke\u2019s cleft cysts: differentiation from other cystic lesions in the pituitary fossa by use of single-shot fast spin-echo diffusion-weighted MR imaging","volume":"149","author":"Kunii","year":"2007","journal-title":"Acta Neurochir. (Wien)"},{"doi-asserted-by":"crossref","unstructured":"Tamura, R., Takahashi, S., Emoto, K., Nagashima, H., Toda, M., and Yoshida, K. (2015). GH-producing pituitary adenoma and concomitant Rathke\u2019s cleft cyst: A case report and short review. Case Rep. Neurol. Med., 948025.","key":"ref_10","DOI":"10.1155\/2015\/948025"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1866","DOI":"10.3174\/ajnr.A4387","article-title":"Differentiation between Cystic Pituitary Adenomas and Rathke Cleft Cysts: A Diagnostic Model Using MRI","volume":"36","author":"Park","year":"2015","journal-title":"AJNR Am. J. Neuroradiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"310","DOI":"10.3171\/2010.7.JNS091793","article-title":"Transsphenoidal resection of 82 Rathke cleft cysts: limited value of alcohol cauterization in reducing recurrence rates","volume":"114","author":"Lillehei","year":"2011","journal-title":"J. Neurosurg."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"3007","DOI":"10.1038\/s41598-019-38542-6","article-title":"Ex Vivo 1H NMR study of pituitary adenomas to differentiate various immunohistochemical subtypes","volume":"9","author":"Ijare","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"14","DOI":"10.6000\/1927-7229.2012.01.01.3","article-title":"Proton magnetic resonance spectroscopy of sputum for the non-invasive diagnosis of lung cancer: Preliminary findings","volume":"1","author":"Bezabeh","year":"2012","journal-title":"J. Anal. Oncol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"9839","DOI":"10.1021\/bi101305b","article-title":"Glycosaminoglycans of the porcine central nervous system","volume":"49","author":"Liu","year":"2010","journal-title":"Biochemistry"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"664","DOI":"10.1016\/0002-9343(69)90162-4","article-title":"Biochemistry and biology of mucopolysaccharides","volume":"47","author":"Meyer","year":"1969","journal-title":"Am. J. Med."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1007\/978-1-4939-1154-7_5","article-title":"Chemistry and function of glycosaminoglycans in the nervous system","volume":"9","author":"Schwartz","year":"2014","journal-title":"Adv. Neurobiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1021\/ac401791h","article-title":"NMR chemical shifts in structural biology of glycosaminoglycans","volume":"86","author":"Pomin","year":"2014","journal-title":"Anal. Chem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"46337","DOI":"10.1074\/jbc.M302529200","article-title":"Evidence of regio-specific glycosylation in human intestinal mucins: Presence of an acidic gradient along the intestinal tract","volume":"278","author":"Robbe","year":"2003","journal-title":"J. Biol. Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/0090-3019(95)00144-1","article-title":"The pathologic, surgical, and MR spectrum of Rathke cleft cysts","volume":"44","author":"Lillehei","year":"1995","journal-title":"Surg. Neurol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"810","DOI":"10.1016\/j.clinimag.2012.01.021","article-title":"Complementary effect of H MRS in diagnosis of suprasellar tumors","volume":"36","author":"Faghih","year":"2012","journal-title":"Clin. Imaging."},{"key":"ref_22","first-page":"TC01","article-title":"Clinical Relevance of Single-Voxel (1)H MRS Metabolites in Discriminating Suprasellar Tumors","volume":"10","author":"Einstien","year":"2016","journal-title":"J. Clin. Diagn. Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1002\/mrm.1160","article-title":"Localized two-dimensional shift correlated MR spectroscopy of human brain","volume":"46","author":"Thomas","year":"2001","journal-title":"Magn. Reson. Med."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"S208","DOI":"10.21037\/gs.2019.06.08","article-title":"High-resolution magnetic resonance imaging at 3T of pituitary gland: advantages and pitfalls","volume":"8","author":"Varrassi","year":"2019","journal-title":"Gland Surg."}],"container-title":["Cancers"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6694\/12\/2\/360\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T08:54:36Z","timestamp":1760172876000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6694\/12\/2\/360"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2,4]]},"references-count":24,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2020,2]]}},"alternative-id":["cancers12020360"],"URL":"https:\/\/doi.org\/10.3390\/cancers12020360","relation":{},"ISSN":["2072-6694"],"issn-type":[{"type":"electronic","value":"2072-6694"}],"subject":[],"published":{"date-parts":[[2020,2,4]]}}}